1. A rapid and sensitive time-resolved fluorescence microsphere immunochromatographic test strip for bongkrekic acid detection in edible fungi.
- Author
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Lin, Wanxin, Sun, Jiadi, Zhang, Ting, Xu, Jia, Huang, Weitao, and Sun, Xiulan
- Subjects
TOXINS ,EDIBLE fungi ,COLLOIDAL gold ,FLUORESCENT probes ,DETECTION limit - Abstract
Bongkrekic acid (BA) is a prevalent biological toxin in black fungus, tremella and fermented rice flour food. BA usually causes acute poisoning. Developing a rapid on-site detection method for BA is essential for timely prevention. This work proposed a time-resolved fluorescent microsphere immunochromatographic test strip (TRFM-ICTS) for the quantitative determination of BA in edible fungi. The process involved synthesizing BA artificial antigens and fluorescent probes. An independent quality control system was implemented. This system ensured the integrity of the control line (C line) even when the test line (T line) was present. Under the optimal conditions, the linear range of TRFM-ICTS was 0.1–10 ng/mL, and the half inhibitory concentration (IC 50) was 0.264 ng/mL. The detection limit of the developed test strip (0.282 ng/mL) was 20 times lower than that of colloidal gold immunochromatographic test strip (5.7 ng/mL). Moreover, TRFM-ICTS showed good recovery in spiked samples, ranging from 81.22% to 119.48%, and the coefficient of variation (CV) was less than 7.76%. In comparison to conventional instrumental techniques, the implemented TRFM-ICTS demonstrated a highly sensitive, straightforward, expeditious, and feasible approach for the quantification of BA in food. • The TRFM-ICTS was designed for BA rapid detection. • The TRFM-ICTS guarantees the control line independent of the test line. • The detection limit of the developed test strip was 0.282 ng/mL. • Excellent performance of strong specificity, high sensitivity and stability. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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