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[Display omitted] • GUP suppressed the activation of multiple inflammasomes. • GUP inhibits inflammasome activation by preventing ASC oligomerization. • GUP inhibits neutrophil release in the LPS-induced acute lung injury model. • GUP may be a candidate drug for the treatment of inflammasome-mediated diseases. Glycyrrhiza uralensis polysaccharides (GUP) has biological activities such as immune regulation, anti-tumor, and liver protection, and has good development prospects in the fields of medicines and health products, but its specific mechanism of action is not yet clear. In our study, we confirmed that GUP can effectively inhibit the activation of the NLRP3 inflammasome. Mechanistically, GUP blocked the activation of the inflammasome by significantly inhibiting the oligomerization and speckle aggregation of the apoptosis-related protein ASC. In addition, GUP effectively ameliorated LPS-induced acute lung injury in mice. Our findings indicate that the Traditional Chinese Medicine (TCM) GUP has broad-spectrum anti-inflammatory effects and is a potential drug candidate for the treatment of inflammasome-related diseases. [ABSTRACT FROM AUTHOR]

In recent years, we have found that the dysregulation of the cyclic-GMP-AMP synthase (cGAS)‒stimulator of interferon genes (STING) pathway leads to the development of immune and inflammatory diseases, therefore, finding compounds that can specifically regulate this pathway is essential for effective regulation of the immune pathway for addressing inflammatory diseases. Licorice flavonoids (LFs), are active ingredients extracted from the Chinese herb licorice, which has been reported to have strong anti-inflammatory activity in previous studies. Here, we report that LFs inhibit the activation of the cGAS-STING pathway evidenced by the inhibition of the expression of type I interferons and related downstream genes such as interferon-stimulated gene 15 (ISG15) and C-X-C motif chemokine ligand 10 (CXCL10), as well as inflammatory cytokines such as interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). Notably, LFs markedly improve the LPS-induced acute lung injury by inhibiting the excessive activation of cGAS-STING signaling pathway. Mechanistically, LFs treatment leads to the blocking of 2′3′-cyclic GMP-AMP (cGAMP) synthesis resulting in an inhibition of the activation of the cGAS-STING pathway. Our results indicate that LFs is a specific inhibitor of the cGAS-STING pathway, which is suggested to be a potential candidate for the treatment of cGAS-STING pathway-mediated inflammatory diseases. [Display omitted] • Licorice flavonoids in licorice can specifically inhibit the cGAS-STING signaling pathway. • Licorice flavonoids inhibit the cGAS-STING pathway by reducing the synthesis of 2′3′-cGAMP. • Licorice flavonoids have ameliorative effects on acute lung injury in mice. [ABSTRACT FROM AUTHOR]

Currently, the number of new cancer cases and deaths worldwide is increasing year on year. In addition to the requirement for cancer prevention, the top priority is still to seek the effective cure of cancer. In over a half century of constant exploration, increasing attention has been paid to the excellent anticancer activity of natural products, with more and more natural products isolated, identified and detected. For this study, the focus lies the natural products of bisindole, where two indole molecules are indirectly linked or directly polymerized, developing the diversity of structure and mechanism, accompanied with the better anticancer activity than monomers. There has been a long history of applying indirubin and vincristine in cancer treatment, verifying the anticancer effect of bisindoles. Vincribine, midostaurin and other anticancer drugs have also been developed and commercialized. In this paper, a review regarding the potential therapeutic effect of bisindole alkaloids extracted from various natural products was carried out, in which the progress made in research of 242 bisindole alkaloids for cancer treatment was introduced. These compounds may be applicable as medicinal products for clinical research in the future. [Display omitted] • 242 bisindole natural products showed different degrees of anticancer activity. • The dimeric indole structure acts as the crux to bisindoles. • Bisindoles provide a variety of leading structures for novel anticancer drugs. [ABSTRACT FROM AUTHOR]