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As our closest living relatives, non-human primates uniquely enable explorations of human health, disease, development, and evolution. Considerable effort has thus been devoted to generating induced pluripotent stem cells (iPSCs) from multiple non-human primate species. Here, we establish improved culture methods for chimpanzee (Pan troglodytes) and pig-tailed macaque (Macaca nemestrina) iPSCs. Such iPSCs spontaneously differentiate in conventional culture conditions, but can be readily propagated by inhibiting endogenous WNT signaling. As a unique functional test of these iPSCs, we injected them into the pre-implantation embryos of another non-human species, rhesus macaques (Macaca mulatta). Ectopic expression of gene BCL2 enhances the survival and proliferation of chimpanzee and pig-tailed macaque iPSCs within the pre-implantation embryo, although the identity and long-term contribution of the transplanted cells warrants further investigation. In summary, we disclose transcriptomic and proteomic data, cell lines, and cell culture resources that may be broadly enabling for non-human primate iPSCs research. [Display omitted] • Inhibiting WNT signaling improves culture of chimp and pig-tailed macaque iPSCs • Endogenous high expression of BCL2 in the rhesus inner cell mass • Expression of BCL2 enhances proliferation of chimp iPSCs in rhesus early embryo • Co-culture of chimp and human iPSCs can differentiate into integrated cardiomyocytes Roodgar et al. demonstrate an improved culture of chimpanzee and pig-tailed macaque iPSCs. They show high expression of endogenous BCL2 in rhesus macaque ICM. Through over expression of BCL2 in chimpanzee iPSCs, they generate primate cross-species pre-implantation embryos composed of chimpanzee iPSCs and rhesus pre-implantation embryos. [ABSTRACT FROM AUTHOR]

Titanium-containing mesoporous molecular sieves with regular pore structure, high specific surface area, tunable pore size and suitable active sites have attracted great attention in the epoxidation of alkenes. Herein, we report the silylated Pd/Ti-MCM-41 (PdTM-ZS) catalysts prepared by treating the series Ti-MCM-41 samples (Si/Ti molar ratio = 10, 15, 20, 25 and 30) with palladium loading followed by surface silylation with trimethylchlorosilane. Reaction results show that the PdTM-ZS catalysts can be applied as efficient catalysts for the selective production of propylene oxide from the oxidation of propylene with cumene hydroperoxide as the oxidant. The PdTM-20S catalyst exhibits an excellent catalytic performance with the selectivity of propylene oxide approaching 100%, which can be attributed to the synergistic effect of abundant active sites, palladium loading and surface silylation. [ABSTRACT FROM AUTHOR]

Developing highly efficient and cost-effective catalyst with tuned microstructure holds great promise in the reduction of nitroaromatic compounds under mild reaction conditions. Herein, we report a new Co@NC-MF catalyst with a fascinating hierarchical flower-like architecture in situ assembled from uniform Co@NC nanoneedles, which can function as a favorable platform for the efficient reduction of nitroaromatic compounds in the presence of NaBH 4. In addition with the structural advantage, the characterization and experimental results demonstrate the enormous advantage of interfacial synergistic catalysis in enhancing the catalytic performance. The outside electron-rich N-doped carbon layer as Lewis basic sites and the inside Co nanoparticles are responsible for the adsorption of 4-nitrophenol (4-NP) and generation of active hydrogen species, respectively. This work contributes to the construction of well-integrated composites with well-balanced interface synergy to boost the catalytic performance in various heterogeneous reactions. ga1 • Co@NC-MF microflowers in situ assembled from Co@NC nanoneedles were constructed. • Co@NC-MF acts as a favorable platform for the reduction of nitroaromatic compounds. • A synergistic catalysis mechanism is proposed based on systematical analysis. • The structural advantage with rich interfacial synergy boosts the performance. [ABSTRACT FROM AUTHOR]