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Background: In recent years, various definitions of "added sugars" have emerged across jurisdictions. Although it is clear how these definitions differ, there is limited understanding of the policy implications associated with these variations. Objective: To test the potential policy implications of different definitions of "added sugars" on the Australian packaged food supply, we developed a method to estimate the content of "added sugars" in packaged foods and applied this to 3 different definitions of "added sugars": (i) United States Food and Drug Administration (US FDA) added sugar definition, (ii) the World Health Organization (WHO) free sugar definition, and (iii) a comprehensive definition that was developed from a review of the evidence on "added sugars." Methods: Using a representative sample of 25,323 Australian packaged foods, the "added sugar" content and proportion of products that contain "added sugar" under the 3 definitions were estimated. In addition, a comparative analysis exploring the impact of the US FDA definition (least comprehensive) vs. the comprehensive definition was conducted to understand potential implications of adopting different regulatory definitions in Australia. Results: The US FDA definition identified the lowest number and proportion of products with any "added sugars" at 14,380 products (representing 56.8% of all products), followed by the WHO free sugar definition at 15,168 products (59.9%) and the comprehensive definition at 16,260 products (64.2%). The mean estimates for "added sugars" were 8.5 g/100 g, 8.7 g/100 g, and 9.6 g/100 g for the US FDA, WHO, and comprehensive definitions, respectively. Compared with the US FDA definition, the comprehensive definition captured an additional 7.4% of products, largely driven by nonalcoholic beverages, special foods and fruit, vegetables, nuts, and legumes. Conclusions: Despite small variations in different "added sugars" definitions, their application has some significant policy implications. Findings highlight the importance of applying a comprehensive regulatory definition that adequately captures all sugars that have been linked to poor health. [ABSTRACT FROM AUTHOR]

In 2020, Google took voluntary action to restrict food and beverage advertising through its online channels in the European Union/United Kingdom using Google's own nutrient profiling model to identify products eligible to be marketed to children through its Google Display Network. The objective of this study was to evaluate the potential impact of the Google policy, if applied to the U.S. market, on restricting online advertising of the top-selling packaged foods and beverages in the U.S. The top 25 U.S. food and beverage manufacturers were identified. Nutrient data for products from these manufacturers were sourced from Label Insight (a Nielsen IQ company) in 2021. Each product was examined against four nutrient profiling models: the Google nutrient profiling model, the WHO Europe nutrient profiling model, the Pan American Health Organization nutrient profiling model, and the Chilean government nutrient profiling model. Under Google's nutrient profiling model, 18% of 14,188 products were eligible to be advertised to children, representing $44 billion in revenue for the top 25 U.S. manufacturers of the >$240 billion generated annually. The Google nutrient profiling model permitted the most products to be advertised to children of all four nutrient profiling models examined. U.S. children engage extensively with online media. In place of government regulation, the Google advertising policy and related nutrient profiling model would limit online advertising of the most unhealthful products to children, if the policy were to be applied to the U.S. market. The effectiveness of the policy would be strengthened by refining the Google nutrient profiling model to better align with nutrient profiling models developed by authoritative health agencies, including the WHO. [ABSTRACT FROM AUTHOR]

Zebra finch songs were studied with regard to interobserver reliability of scoring sonagrams by eye.

Plants detect potential pathogens by sensing microbe-associated molecular patterns via pattern recognition receptors. In the dicot model plant Arabidopsis, the lysin motif (LysM)-containing chitin elicitor receptor kinase 1 (CERK1) has been shown to be essential for perception of the fungal cell wall component chitin and for resistance to fungal pathogens. Recent in vitro studies with CERK1 protein expressed heterologously in yeast suggested direct chitin binding activity. Here we show in an affinity purification approach that CERK1 is a major chitin-binding protein of Arabidopsis cells, along with several known and putative chitinases. The ectodomain of CERK1 harbors three distinct LysM domains with potential ligand binding capacity. We demonstrate that the CERK1 ectodomain binds chitin and partially deacetylated chitosan directly without any requirement for interacting proteins and that all three LysM domains are necessary for chitin binding. Ligand-induced phosphorylation events are a general feature of animal and plant signal transduction pathways. Our studies show that chitin, chitin oligomers, and chitosan rapidly induce in vivo phosphorylation of CERK1 at multiple residues in the juxtamembrane and kinase domain. Functional analyses with a kinase dead variant provide evidence that kinase activity of CERK1 is required for its chitin-dependent in vivo phosphorylation, as well as for early defense responses and downstream signaling. Collectively, our data suggest that in Arabidopsis, CERK1 is a major chitin, chitosan, and chito-oligomer binding component and that chitin signaling depends on CERK1 post-translational modification and kinase activity. [ABSTRACT FROM AUTHOR]

The National Languages Strategy, published in the UK in 2002 offers all primary school children an entitlement to learn a Modern Foreign Language (MFL) by 2010. The supply of adequately trained teachers is one of the key determinants of the success of this initiative. This paper presents findings from a study involving 18 teacher education institutions offering a primary MFL specialism, focusing particularly on the preservice teachers’ confidence in their own subject knowledge. A compulsory component of the specialism is a month of practicum abroad and this article explores the potential benefits and difficulties associated with this experience, as reported by the preservice teachers through questionnaires and focus groups. [Copyright &y& Elsevier]

Alterations in the proteome of Arabidopsis thaliana leaves during early responses to challenge by Pseudomonas syringae pv. tomato DC3000 (DC3000) were analysed using two-dimensional (2D) gel electrophoresis. Protein changes characteristic of the establishment of basal resistance and R-gene mediated resistance were examined by comparing responses to DC3000, a hrp mutant and DC3000 expressing avrRpm1 respectively. The abundance of selected transcripts was also analysed in GeneChip experiments. Here we present data from the soluble fraction of leaf protein, highlighting changes in two antioxidant enzyme groups; the glutathione S-transferases (GSTs F2, F6, F7 and F8) and peroxiredoxins (PrxA, B and IIE). Members of both enzyme groups showed signs of specific post-translational modifications, represented by multiple spots on gels. We suggest that oxidation of specific residues is responsible for some of the spot shifts. All forms of the GST proteins identified here increased following inoculation with bacteria. GSTF8 showed particularly dynamic responses to pathogen challenge, the corresponding transcript was significantly up-regulated by 2 h after inoculation, and the protein showed post-translational modifications specific to an incompatible interaction. Differential changes were observed with the peroxiredoxin proteins; PrxIIE and to a lesser extent PrxB, no change was observed with PrxA, but a truncated form PrxA-L was greatly reduced in abundance following bacterial challenges. Our data suggest that bacterial challenge generally induces Prxs and the antioxidants GSTs, however individual members of these families may be specifically modified dependent upon the virulence of the DC3000 strain and outcome of the interaction. Finally, proteomic and transcriptomic data derived from the same inoculation system are compared and the advantages offered by 2D gel analysis discussed in light of our results. [Copyright &y& Elsevier]

During carcinoma progression, tumor cells often undergo changes similar (but not identical) to epithelial. mesenchymal transitions in embryonic development. In this study, we demonstrate that experimental stimulation of hyaluronan synthesis in normal epithelial cells is sufficient to induce mesenchymal and transformed characteristics. Using recombinant adenoviral expression of hyaluronan synthase-2, we show that increased hyaluronan production promotes anchorage-independent growth and invasiveness, induces gelatinase production, and stimulates phosphoinositide 3-kinase/Akt pathway activity in phenotypically normal MadinDarby canine kidney and MCF-10A human mammary epithelial cells. Cells infected with hyaluronan synthase-2 adenovirus also acquired mesenchymal characteristics, including up-regulation of vimentin, dispersion of cytokeratin, and loss of organized adhesion proteins at intercellular boundaries. Furthermore, we show that the transforming effects of two well described agents, hepatocyte growth factor (HGF) and β-catenin, are dependent on hyaluronan-cell interactions. Perturbation of endogenous hyaluronan polymer interactions by treatment with hyaluronan oligomers is shown here to reverse the transforming effects of HGF and β-catenin in Madin-Darby canine kidney and MCF-10A human mammary epithelial cells. Also, HGF and β-catenin induced assembly of hyaluronan-dependent pericellular matrices similar to those surrounding mesenchymal cells. Thus, increased expression of hyaluronan is sufficient to induce epithelial-mesenchymal transition and acquisition of transformed properties in phenotypically normal epithelial cells. [ABSTRACT FROM AUTHOR]

Autophagy is the degradation of cytoplasmic material through the lysosomal pathway. One of the most studied autophagy-related proteins is LC3. Despite growing evidence that LC3 is enriched in the nucleus, its nuclear role is poorly understood. Here, we show that Drosophila Atg8a protein, homologous to mammalian LC3, interacts with the transcription factor Sequoia in a LIR motif-dependent manner. We show that Sequoia depletion induces autophagy in nutrient-rich conditions through the enhanced expression of autophagy genes. We show that Atg8a interacts with YL-1, a component of a nuclear acetyltransferase complex, and that it is acetylated in nutrient-rich conditions. We also show that Atg8a interacts with the deacetylase Sir2, which deacetylates Atg8a during starvation to activate autophagy. Our results suggest a mechanism of regulation of the expression of autophagy genes by Atg8a, which is linked to its acetylation status and its interaction with Sequoia, YL-1, and Sir2. • Transcription factor Sequoia is a negative regulator of autophagy • Sequoia interacts with Atg8a via a LIR motif • Atg8a interacts with YL-1, a subunit of a nuclear acetyltransferase complex • Sir2 interacts with and deacetylates Atg8a during starvation Jacomin et al. show that Atg8a interacts with transcription factor Sequoia. Depletion of Sequoia induces autophagy through enhanced expression of autophagy genes. Atg8a also interacts with YL-1, a component of a nuclear acetyltransferase complex, and deacetylase Sir2. Sir2 deacetylates Atg8a during starvation to activate autophagy. [ABSTRACT FROM AUTHOR]

• Different front-of-pack food labels have varying ability to assist consumers. • Coloured, spectrum labels were found to be of most assistance in 18 countries. • These labels were best at identifying both the healthiest and least healthy options. • Colour appears to be a critical element of effective front-of-pack nutrition labels. Various front-of-pack food labels (FoPLs) are being introduced across the world, and discussion continues about the most effective label formats to improve consumers' (i) understanding of the relative healthiness of alternative products and (ii) product choices. Of increasing interest is the relative ability of different types of labels to steer consumers away from unhealthy options (aversion) versus steer them towards healthier options (attraction). The aim of this study was to assess aversion and attraction outcomes for five FoPLs (Health Star Rating, Multiple Traffic Lights, Nutri-Score, Reference Intakes, and Warning Label) across 18 countries (n = 18393). Descriptive analyses assessed improvements in consumer understanding and choice outcomes for each label across three different types of food products. Binary logistic regressions were used to compare the relative ability of the labels to improve respondents' understanding of product healthiness and their product choices, using the industry-developed Reference Intakes as the comparator. Aversion and attraction effects were assessed for each label/food type combination. Across the total sample, the Nutri-Score performed best in terms of both attraction and aversion results for understanding and simulated choice outcomes, followed by the Multiple Traffic Lights. The Reference Intakes exhibited the weakest performance overall, with the Warning Label and Health Star Rating falling in between. The most effective FoPLs featured a colour-coded spectrum design. The results indicate that front-of-pack labels that are effective in guiding consumers towards healthier food products can also be effective in steering them away from unhealthy options. [ABSTRACT FROM AUTHOR]

Focuses on the link of multidrug resistance in cancer cells due to the use of adenosine triphosphate-dependent efflux pumps. Effect of alteration in cell survival and apoptotic signaling pathways; Reduction of tumor growth by perturbation of hyaluronan-tumor cell interaction; Sensitization of tumor cells to chemotherapeutic drugs.

In parallel with growing consumer interest in reducing sugar intake, manufacturers have increased availability of food and beverage products containing nonnutritive sweeteners (NNSs). However, emerging evidence indicates that specific NNS types have differential effects on cardiometabolic health. This study examined overall changes in the presence of NNSs, sugar alcohols, and free sugars in the Australian food supply and the use of specific NNS types. Data for 21,051 products in 2015 and 21,366 products in 2019 were extracted from The George Institute's FoodSwitch database. The proportion of products containing NNSs, sugar alcohols, free sugars, and a combination of these, as well as proportion of products containing specific NNS types. Changes between 2015 and 2019 were examined using Pearson χ 2 tests. Between 2015 and 2019, there was a significant increase in the proportion of food and beverage products containing NNSs (from 3.8% to 4.3%; P <.001) and a significant decrease in products containing free sugars (from 62.7% to 59.9%; P <.001),) driven primarily by nondairy beverages. There were changes in the use of specific NNS types between 2015 and 2019, with a large increase in the use of steviol glycosides (from 33.7% to 50.2%) and a large decrease in the use of sucralose (from 42.4% to 30.5%), aspartame (from 21.0% to 14.4%), and acesulfame K (from 57.4% to 27.7%) (P <.05 for all). These findings on the use of different NNS, sugar alcohol, and free sugar ingredients and combinations provide important research insights and will be useful in informing government policies that address sugars and other sweeteners in Australian foods. [ABSTRACT FROM AUTHOR]

Recently, 3D-graphene infused polyimide (3DC/PI) films have shown to be an effective protection coating for electrostatic discharge in spacecraft. However, these films are not suitable for Low Earth Orbit (LEO) due to atomic oxygen (AO) erosion. Here, we used Polyhedral Oligomeric Silsesquioxane (POSS) to enhance the AO durability of 3D-C/PI films. Three different ways of adding POSS to the composite films were studied with ground-based AO exposure. For all infusion approaches, their electrical conductivity behaviour is well preserved and the presence of POSS results in reduced AO erosion yield. Of all the methods studied here, incorporating POSS directly into PI results in the lowest erosion yield of 4.67 × 10−25 cm3/O-atom (one order of magnitude lower than that of Kapton). Adding POSS to PI, extends the durability of the composite film beyond 10 years, making it an ideal protective material for long term mission in LEO. Image 1 • Comparison of AO erosion of 3D-C/PI films with POSS in 3D-C or PI or both. • POSS added to 3D-C alone has no effect on composite atomic oxygen erosion yield. • Adding POSS to PI, extends the lifetime of 3D-C/PI film beyond 10 years in LEO. • 5 wt% of POSS added to PI increases the Youngs's modulus of 3D-C/PI. • 3D-C/PI with 15 wt% of POSS has lower modulus than 3D-C/PI with 5 wt% POSS. [ABSTRACT FROM AUTHOR]