7 results on '"Kim, Tae-Don"'
Search Results
2. The SpACE-CCM: A facile and versatile cell culture medium-based biosensor for detection of SARS-CoV-2 spike-ACE2 interaction.
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Ham, Youngwook, Cho, Nam-Chul, Kim, Daeyong, Kim, Jung-Hee, Jo, Min Ju, Jeong, Min Seon, Pak, Bo-Yeong, Lee, Sanghyeok, Lee, Mi-Kyung, Chi, Seung-Wook, Kim, Tae-Don, Jeong, Nak Cheol, and Cho, Sungchan
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SARS-CoV-2 , *LUCIFERASES , *BIOSENSORS , *CELL culture - Abstract
The COVID-19 pandemic is an ongoing global public health threat. COVID-19 is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, and binding of the SARS-CoV-2 spike to its receptor, angiotensin-converting enzyme 2 (ACE2), on host cells is critical for viral infection. Here, we developed a luminescent biosensor that readily detects interactions of the sp ike receptor-binding domain (RBD) and ACE 2 in c ell c ulture m edium ('SpACE-CCM'), which was based on bimolecular complementation of the split nanoluciferase-fused spike RBD and ectodomain of ACE2 and further engineered to be efficiently secreted from cells by adding a heterologous secretory signal peptide (SSP). Screening of various SSPs identified 'interferon-α+alanine-aspartate' as the SSP that induced the highest activity. The SpACE-CCM biosensor was validated by observing a marked reduction of the activity caused by interaction-defective mutations or in the presence of neutralizing antibodies, recombinant decoy proteins, or peptides. Importantly, the SpACE-CCM biosensor responded well in assay-validating conditions compared with conventional cell lysate-based NanoLuc Binary Technology, indicating its advantage. We further demonstrated the biosensor's versatility by quantitatively detecting neutralizing activity in blood samples from COVID-19 patients and vaccinated individuals, discovering a small molecule interfering with the spike RBD-ACE2 interaction through high-throughput screening, and assessing the cross-reactivity of neutralizing antibodies against SARS-CoV-2 variants. Because the SpACE-CCM is a facile and rapid one-step reaction biosensor that aptly recapitulates the native spike-ACE2 interaction, it would be advantageous in many experimental and clinical applications associated with this interaction. [ABSTRACT FROM AUTHOR]
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- 2023
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3. Macrophage migration inhibitory factor interacts with thioredoxin-interacting protein and induces NF-κB activity.
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Kim, Mi Jeong, Kim, Won Sam, Kim, Dong Oh, Byun, Jae-Eun, Huy, Hangsak, Lee, Soo Yun, Song, Hae Young, Park, Young-Jun, Kim, Tae-Don, Yoon, Suk Ran, Choi, Eun-Ji, Ha, Hyunjung, Jung, Haiyoung, and Choi, Inpyo
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CANCER cell migration , *MACROPHAGES , *THIOREDOXIN-interacting protein , *NF-kappa B , *NEOPLASTIC cell transformation - Abstract
The nuclear factor kappa B (NF-κB) pathway is pivotal in controlling survival and apoptosis of cancer cells. Macrophage migration inhibitory factor (MIF), a cytokine that regulates the immune response and tumorigenesis under inflammatory conditions, is upregulated in various tumors. However, the intracellular functions of MIF are unclear. In this study, we found that MIF directly interacted with thioredoxin-interacting protein (TXNIP), a tumor suppressor and known inhibitor of NF-κB activity, and MIF significantly induced NF-κB activation. MIF competed with TXNIP for NF-κB activation, and the intracellular MIF induced NF-κB target genes, including c -IAP2, Bcl-xL, ICAM-1, MMP2 and uPA, by inhibiting the interactions between TXNIP and HDACs or p65. Furthermore, we identified the interaction motifs between MIF and TXNIP via site-directed mutagenesis of their cysteine (Cys) residues. Cys 57 and Cys 81 of MIF and Cys 36 and Cys 120 of TXNIP were responsible for the interaction. MIF reversed the TXNIP-induced suppression of cell proliferation and migration. Overall, we suggest that MIF induces NF-κB activity by counter acting the inhibitory effect of TXNIP on the NF-κB pathway via direct interaction with TXNIP. These findings reveal a novel intracellular function of MIF in the progression of cancer. [ABSTRACT FROM AUTHOR]
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- 2017
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4. Ginsenoside 20(R)-Rg3 enhances natural killer cell activity by increasing activating receptor expression through the MAPK/ERK signaling pathway.
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Lee, Yunhee, Park, Arum, Park, Young-Jun, Jung, Haiyoung, Kim, Tae-Don, Noh, Ji-Yoon, Choi, Inpyo, Lee, Seungjin, and Ran Yoon, Suk
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KILLER cell receptors , *EXTRACELLULAR signal-regulated kinases , *GINSENOSIDES , *CELLULAR signal transduction , *MITOGEN-activated protein kinases , *KILLER cells , *CELL physiology - Abstract
• Ginsenoside Rg3 increasd NK cell cytolytic activity. • Ginsenoside Rg3 activated NK cells through the MAPK/ERK signaling pathway. • Ginsenoside Rg3 enhanced the functional maturation of CB-derived NK cells. • Ginsenoside 20(R)-Rg3 was effective at activating NK cells and was activated through the MAPK/ERK signaling pathway. Ginseng is one of the most widely used herbal remedies for various diseases worldwide. Ginsenoside Rg3 (G-Rg3), the main component of ginseng, possesses several pharmacological properties, including anti-inflammatory, anti-tumor, antioxidant, anti-obesity, and immunomodulatory activities. However, the effect of G-Rg3 on natural killer (NK) cells in humans is not fully understood. Here, we investigated the effect of G-Rg3 on NK cell function and differentiation and elucidated the underlying mechanism. G-Rg3 increased NK cell cytotoxicity and simultaneously increased the expression of NK-activating receptors, NKp44, NKp46, and NKp30. Additionally, G-Rg3 increased the mRNA expression of NK cytolytic molecules, granzyme B and perforin. The expression of CD107a, a marker of NK cell degranulation, also increased in G-Rg3–treated NK cells. We therefore proceeded to identify which MAPK signaling pathway was involved in G-Rg3–mediated cytolytic activity. Treatment with G-Rg3 increased the phosphorylation levels of extracellular signal–regulated kinase (ERK), whereas ERK inhibition eliminated G-Rg3–induced NK cell cytotoxicity, suggesting the involvement of the ERK pathway. G-Rg3 did not affect the rate of differentiation of human cord-blood–derived NK cells; however, it increased the functional maturation of differentiated NK cells and promoted their cytotoxicity. The G-Rg3 isomer, 20(R)-Rg3, effectively activated NK cells via the extracellular signal-regulated kinase (ERK) signaling pathway, whereas 20(S)-Rg3 had no effect on NK cell activity. Altogether, the results demonstrated that 20(R)-Rg3 promoted NK cell activity via activation of the MAPK/ERK pathway, suggesting that 20(R)-Rg3 may be used as an activator of NK cell cytotoxicity for the treatment of diverse types of cancers. [ABSTRACT FROM AUTHOR]
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- 2022
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5. TOX regulates the differentiation of human natural killer cells from hematopoietic stem cells in vitro
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Yun, Sohyun, Lee, Suk Hyung, Yoon, Suk-Ran, Kim, Mi Sun, Piao, Zheng-Hao, Myung, Pyung-Keun, Kim, Tae-Don, Jung, Haiyoung, and Choi, Inpyo
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UBIQUITIN , *KILLER cells , *T cell differentiation , *HEMATOPOIETIC stem cells , *NATURAL immunity , *CELL receptors , *BIOMARKERS - Abstract
Abstract: Natural killer (NK) cells act important roles in innate immunity and adaptive immunity. However, the mechanisms governing NK cell development have not been clearly elucidated. Previous studies have shown that an HMG (high-mobility group) protein, TOX, is important for regulating the differentiation program of developing T cells in mice. In this study, we examined the role of TOX in differentiation of human NK cells. Knockdown of TOX in differentiating cells decreased the NK cell population identified by expression of NK surface markers and receptors. In addition, over-expression of TOX enhanced the differentiation of NK cells which give rise to a population showing effector functions of mature NK cells. Moreover, TOX influenced expression of T-bet (T-box expressed in T cells, also as known as Tbx21) during NK cell development. Overall, these results suggest that TOX is required for IL-15-mediated NK cell differentiation and affected expression of T-bet that plays critical roles in NK differentiation and maturation. [Copyright &y& Elsevier]
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- 2011
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6. YC-1 enhances natural killer cell differentiation from hematopoietic stem cells
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Yun, Sohyun, Lee, Suk Hyung, Kang, Yun Hee, Jeong, Mira, Kim, Mi Jeong, Kim, Mi Sun, Piao, Zheng-Hao, Suh, Hyun-Woo, Kim, Tae-don, Myung, Pyung-Keun, Yoon, Suk-Ran, and Choi, Inpyo
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THERAPEUTIC use of nitric oxide , *KILLER cells , *CELL differentiation , *HEMATOPOIETIC stem cells , *NATURAL immunity , *CELL growth , *CELLULAR signal transduction - Abstract
Abstract: NK cells play crucial roles in innate immunity and adaptive immunity. The detailed mechanisms, however, governing NK cell development remains unclear. In this study, we report that YC-1 significantly enhances NK cell populations differentiated from human umbilical cord blood hematopoietic stem cells (HSCs). NK cells increased by YC-1 display both phenotypic and functional features of fully mature NK (mNK) cells, but YC-1 does not affect the activation of mNK cells. YC-1 did not affect cGMP production and phosphorylation of STAT-5 which is essential for IL-15R signaling. On the other hand, YC-1 increased p38 MAPK phosphorylation during NK cell differentiation. Furthermore, p38 inhibitor SB203580 inhibited the differentiation of NK cells enhanced by YC-1. Taken together, these data suggest that YC-1 enhances NK cell differentiation through the activation of p38 MAPK which is involved in NK cell differentiation. [Copyright &y& Elsevier]
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- 2010
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7. RNA demethylation by FTO stabilizes the FOXJ1 mRNA for proper motile ciliogenesis.
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Kim, Hyunjoon, Lee, Young-suk, Kim, Seok-Min, Jang, Soohyun, Choi, Hyunji, Lee, Jae-Won, Kim, Tae-Don, and Kim, V. Narry
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DEMETHYLATION , *MESSENGER RNA , *RNA , *XENOPUS laevis , *ALLERGENS , *EMBRYOLOGY , *SOX2 protein - Abstract
Adenosine N6-methylation (m6A) is one of the most pervasive mRNA modifications, and yet the physiological significance of m6A removal (demethylation) remains elusive. Here, we report that the m6A demethylase FTO functions as a conserved regulator of motile ciliogenesis. Mechanistically, FTO demethylates and thereby stabilizes the mRNA that encodes the master ciliary transcription factor FOXJ1. Depletion of Fto in Xenopus laevis embryos caused widespread motile cilia defects, and Foxj1 was identified as one of the major phenocritical targets. In primary human airway epithelium, FTO depletion also led to FOXJ1 mRNA destabilization and a severe loss of ciliated cells with an increase of neighboring goblet cells. Consistently, Fto knockout mice showed strong asthma-like phenotypes upon allergen challenge, a result owing to defective ciliated cells in the airway epithelium. Altogether, our study reveals a conserved role of the FTO-FOXJ1 axis in embryonic and homeostatic motile ciliogenesis. [Display omitted] • FTO is required during motile ciliogenesis in a m6A-dependent manner • FTO demethylates and stabilizes the mRNA of transcription factor FOXJ1 • FTO-FOXJ1 axis coordinates the formation of the mammalian airway epithelium • RNA demethylation is critical for motile ciliogenesis in development and homeostasis Kim, Lee et al. examine the physiological significance of RNA demethylation in embryonic development and tissue homeostasis. They show that the m6A demethylase FTO targets the FOXJ1 mRNA to enhance its stability. This posttranscriptional FTO-FOXJ1 axis is essential for proper motile ciliogenesis in frogs, mice, and humans. [ABSTRACT FROM AUTHOR]
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- 2021
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