7 results on '"Langlois, V.S."'
Search Results
2. Polycyclic aromatic compounds (PACs) in the Canadian environment: The challenges of ecological risk assessments.
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Hodson, P.V., Wallace, S.J., de Solla, S.R., Head, S.J., Hepditch, S.L.J., Parrott, J.L., Thomas, P.J., Berthiaume, A., and Langlois, V.S.
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ECOLOGICAL risk assessment ,POLYCYCLIC aromatic compounds ,TOXAPHENE ,HAZARDOUS waste sites ,ENVIRONMENTAL quality ,CYTOCHROME P-450 - Abstract
Ecological risk assessments (ERAs) of polycyclic aromatic compounds (PACs), as single congeners or in mixtures, present technical challenges that raise concerns about their accuracy and validity for Canadian environments. Of more than 100,000 possible PAC structures, the toxicity of fewer than 1% have been tested as individual compounds, limiting the assessment of complex mixtures. Because of the diversity in modes of PAC action, the additivity of mixtures cannot be assumed, and mixture compositions change rapidly with weathering. In vertebrates, PACs are rapidly oxygenated by cytochrome P450 enzymes, often to metabolites that are more toxic than the parent compound. The ability to predict the ecological fate, distribution and effects of PACs is limited by toxicity data derived from tests of a few responses with a limited array of test species, under optimal laboratory conditions. Although several models are available to predict PAC toxicity and rank species sensitivity, they were developed with data biased by test methods, and the reported toxicities of many PACs exceed their solubility limits. As a result, Canadian Environmental Quality Guidelines for a few individual PACs provide little support for ERAs of complex mixtures in emissions and at contaminated sites. These issues are illustrated by reviews of three case studies of PAC-contaminated sites relevant to Canadian ecosystems. Interactions among ecosystem characteristics, the behaviour, fate and distribution of PACs, and non-chemical stresses on PAC-exposed species prevented clear associations between cause and effect. The uncertainties of ERAs can only be reduced by estimating the toxicity of a wider array of PACs to species typical of Canada's diverse geography and environmental conditions. Improvements are needed to models that predict toxicity, and more field studies of contaminated sites in Canada are needed to understand the ecological effects of PAC mixtures. Image 1 • ERAs for PACS in Canada are hampered by a lack of relevant data. • ERAs must deal with a diverse array of >100,000 PACs; the toxicity of <1% is known. • The sensitivity to PACs has been assessed for <1% of global species. • PACs occur in complex mixtures of compounds with multiple modes of action. • Research is needed on MOAs, mixture interactions and models of PAC toxicity. This review assesses the challenges of ecological risk assessments for polycyclic aromatic compounds due to complex interactions among a diversity of PAC structures, exposures, and environmental receptors in Canadian ecosystems. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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3. Polycyclic aromatic compounds (PACs) in the Canadian environment: Exposure and effects on wildlife.
- Author
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Wallace, S.J., de Solla, S.R., Head, J.A., Hodson, P.V., Parrott, J.L., Thomas, P.J., Berthiaume, A., and Langlois, V.S.
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POLYCYCLIC aromatic compounds ,REPTILES ,AQUATIC mammals ,POLYCYCLIC aromatic hydrocarbons ,ANIMALS ,OIL sands ,INDUSTRIAL sites - Abstract
Polycyclic aromatic compounds (PACs) are ubiquitous in the environment. Wildlife (including fish) are chronically exposed to PACs through air, water, sediment, soil, and/or dietary routes. Exposures are highest near industrial or urban sites, such as aluminum smelters and oil sands mines, or near natural sources such as forest fires. This review assesses the exposure and toxicity of PACs to wildlife, with a focus on the Canadian environment. Most published field studies measured PAC concentrations in tissues of invertebrates, fish, and birds, with fewer studies of amphibians and mammals. In general, PAC concentrations measured in Canadian wildlife tissues were under the benzo[a]pyrene (BaP) guideline for human consumption. Health effects of PAC exposure include embryotoxicity, deformities, cardiotoxicity, DNA damage, changes to DNA methylation, oxidative stress, endocrine disruption, and impaired reproduction. Much of the toxicity of PACs can be attributed to their bioavailability, and the extent to which certain PACs are transformed into more toxic metabolites by cytochrome P450 enzymes. As most mechanistic studies are limited to individual polycyclic aromatic hydrocarbons (PAHs), particularly BaP, research on other PACs and PAC-containing complex mixtures is required to understand the environmental significance of PAC exposure and toxicity. Additional work on responses to PACs in amphibians, reptiles, and semi-aquatic mammals, and development of molecular markers for early detection of biological responses to PACs would provide a stronger biological and ecological justification for regulating PAC emissions to protect Canadian wildlife. Image 1 • Most studies measured polycyclic aromatic compounds (PAC) in invertebrates and fish. • In general, PAC concentrations in Canadian wildlife tissue were below guidelines. • Mechanisms of PAC toxicity are similar among species but some unique effects exist. • Best to measure biological effects with environmental and tissue PAC exposure. This paper reviews literature on the exposure and effects of PACs on wildlife and ecosystems, with a special focus on Canadian invertebrates, fish, amphibians, reptiles, birds, and small mammals. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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4. Sub-lethal effects of calcium dinonylnaphthalenesulfonate on Western clawed frog embryos.
- Author
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Wallace, S.J., Leclerc, A.J.A., Prosser, R., de Solla, S.R., Balakrishnan, V., and Langlois, V.S.
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GLUTATHIONE peroxidase ,ESSENTIAL amino acids ,AMINO acid metabolism ,FROGS ,EMBRYOS ,GLUTATHIONE reductase - Abstract
Naphthalene sulfonic acids (NSAs) are used as additives in lubricants, dyes, and greases and commonly act as surfactants in many industrial processes. The calcium salt of dinonyl NSA (calcium dinonylnaphthalenesulfonate; CaDNS) is listed among thousands of chemicals identified as priorities for assessment by the Government of Canada's Chemical Management Plan due to the limited toxicity data. The purpose of this study was two-fold: 1) to establish the toxicity of CaDNS to Western clawed frog (Silurana tropicalis) embryos and 2) to assess the sub-lethal effects and mechanisms of toxicity of CaDNS in amphibians through targeted gene expression and metabolite analyses. Frog embryos were exposed to water overlying sand spiked with a range of concentrations of CaDNS (17–1393 μg/g) over a 72-h period. Results indicated significantly higher mortality and presence of malformations in frog larvae exposed to over 672 μg/g CaDNS in the sand (14 ng/mL CaDNS in the water) compared to control treatments. An overall decrease in the glutathione redox cycle was observed, including decreases in relative mRNA levels of enzymes (glutathione S-transferase (gst), glutathione reductase (gsr), glutathione peroxidase (gpx)) and decreases in the glutathione (GSH) and glutathione disulfide (GSSG) metabolite concentrations. In addition, transcript levels of genes involved in antioxidant capacity and essential amino acid metabolites decreased significantly in embryos exposed to low levels of CaDNS. This is the first study to assess the toxicity of NSAs in amphibians, contributing important data to aid in the assessment of NSAs. Unlabelled Image • First study of toxicity of naphthalene sulfonic acid, calcium salt in amphibians • Frog embryos exposed to low levels are developmentally impaired and malformed. • Overall decrease in gene expression and metabolites in the glutathione redox cycle • Decrease in antioxidant capacity and essential amino acid metabolism [ABSTRACT FROM AUTHOR]
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- 2020
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5. Aquatic toxicity and chemical fate of diluted bitumen spills in freshwater under natural weathering.
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Hepditch, S.L.J., Gutierrez-Villagomez, J.M., To, T.A., Larocque, E., Xin, Q., Heshka, N., Vander Meulen, I., Headley, J.V., Dettman, H.D., Triffault-Bouchet, G., Ahad, J.M.E., and Langlois, V.S.
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HEAVY oil , *ORGANIC acids , *ARYL hydrocarbon receptors , *POLYCYCLIC aromatic compounds , *AQUATIC resources - Abstract
Increasing global demands for oils are fueling the production of diluted bitumen (DB) from Canada's oil sands region. More weathered than conventional crude (CC) oils, Alberta bitumen is often diluted with lighter petroleum oils to reduce density and viscosity to meet pipeline specifications for transportation. Being a heavy oil product that is transported in large volumes across Canada and the USA, there has been interest to compare its behavior and toxicity characteristics when spilled to those of CC. To determine the influence of environmental weathering upon DB following a freshwater spill, we conducted separate controlled spills of Cold Lake Blend DB and Mixed Sweet Blend light CC oil in a mesocosm spill-tank system at 24 °C with wave-action for 56 days. DB-contaminated waters remained acutely lethal for a period of 14 days to early life stage fathead minnows (Pimephales promelas) exposed during embryologic development, while CC was lethal for 1 day. However, concentrations of mono- and polycyclic aromatic compounds, often claimed to be principally responsible for the acute and chronic toxicity of crude oils, were consistently higher in CC water compared to DB. Elevated aromatic concentrations in CC water correlated with higher prevalences of developmental malformations, reduced heart and growth rates, and impacts on the aryl hydrocarbon receptor pathway. Organic acids were measured over the course of the studies and O 2 containing naphthenic acids were present at greater relative abundances in DB- compared to CC-contaminated water, with their attenuation correlating with reduced acute and sublethal toxicity. Furthermore, organic acid degradation products accumulated with time and likely contributed to the consistently sublethal toxicity of the weathered oils throughout the experiment. Improved characterization of the fractions including organic acids and those organic compounds found within the unresolved complex mixture of fresh and weathered crude oils is necessary to adequately understand and prepare for the risks that accidental petroleum spills pose to aquatic resources. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
6. Diluted bitumen causes deformities and molecular responses indicative of oxidative stress in Japanese medaka embryos.
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Madison, Barry N., Hodson, P.V., and Langlois, V.S.
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BITUMEN , *OXIDATIVE stress , *ORYZIAS latipes , *FISH embryos , *MESSENGER RNA , *XENOBIOTICS , *FISHES - Abstract
This study characterized the toxicity and physiological effects of unweathered diluted bitumen (Access Western Blend dilbit; AWB) to fish. Embryos of Japanese medaka ( Oryzias latipes ) were exposed for 17 days to dilutions of physically-dispersed (water accommodated fraction; WAF) and chemically-dispersed (chemically-enhanced WAF; CEWAF) dilbit. AWB dilbit exposure was not lethal to medaka, but resulted in a high prevalence of blue sac disease (BSD), impaired development, and abnormal or un-inflated swim bladders at hatch. Physiological effects were indicated by the relative mRNA levels of key genes associated with, among others, cell cycling and the response to mutations ( p53 ), xenobiotic metabolism ( ahr , arnt2 ), phase I ( cyp1a ) and II processes associated with oxidative stress ( cat, g6pdh, hsp70, gst, gpx, gsr, nfe2, and sod ). AWB dilbit treatment increased p53 and cyp1a transcript levels (1.5-fold and >15-fold, respectively), with significant, but less pronounced changes in indicators of oxidative stress and metabolism. The exposure-related changes in embryotoxicity and mRNA synthesis were consistent with metabolism of polycyclic aromatic hydrocarbons (PAHs) to reactive and toxic metabolites. Medaka embryos responded similarly to WAF and CEWAF treatments, but CEWAF was about 100 times more efficient in delivering toxic concentrations of PAHs. The toxicity of chemically-dispersed nujol, a non-toxic mineral oil used as an experimental control, suggested that a portion of the observed effects of AWB could be attributed to excess dispersant in solution. This first study of the physiological effects of dilbit toxicity to fish embryos provides a baseline to compare toxicity between dilbit and conventional crude oils, and the groundwork for the development of molecular biomarkers of the sensitivity and level of risk of native Canadian fish species to dilbit exposure. [ABSTRACT FROM AUTHOR]
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- 2015
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7. Morphological and molecular effects of two diluted bitumens on developing fathead minnow (Pimephales promelas).
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Alsaadi, F.M., Madison, B.N., Brown, R.S., Hodson, P.V., and Langlois, V.S.
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FATHEAD minnow , *POLYCYCLIC aromatic compounds , *BITUMEN , *OIL sands , *ZEBRA danio embryos , *NATIVE fishes , *FELIDAE - Abstract
Abstract Canada has experienced a significant increase in the transport of diluted bitumen (dilbit), a predominant oil sands product that combines bitumen with diluents derived from oil-gas condensates and other proprietary compounds. The toxicity of dilbit to fish embryos, which are immobile and thus at a high risk of exposure to oil in the event of a spill, remains largely unknown for most species. This study assessed the toxicity of water accommodated fractions (WAF) and chemically enhanced water accommodated fractions (CEWAF) of two winter dilbit blends, Access Western Blend (AWB) and Cold Lake Blend (CLB), to fathead minnow (Pimephales promelas) embryos. The TPH-F EC50s for malformations were 834 and 1058 μg/L for AWB WAF and CEWAF, respectively, and 500 and 715 μg/L for CLB WAF and CEWAF, respectively. Levels of cyp1a mRNA increased up to 46- and 69-fold, respectively, reflecting increasing exposure to polycyclic aromatic compounds (PACs) in AWB and CLB. Similarly, levels of gst mRNA were elevated up to 3.8-fold and 2.7-fold with increasing total concentrations of PACs in AWB and CLB, respectively. However, there were no significant changes in mRNA levels of p53 , sod, cat , and gsr. These results suggest that the expression of cyp1a and gst may serve as biomarkers for dilbit exposure in fathead minnow, furthering our understanding of dilbit-responsive indicators of toxicity in fish species native to North America. This study is important as it utilizes the same exposure methodology to examine the toxicity of two commonly used Canadian dilbits, facilitating comparison of dilbit toxicity. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
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