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Salinization of land is globally increasing due to climate change, and salinity stress is an important abiotic stressor that adversely affects agricultural productivity. In this study, we assessed a halotolerant endophytic bacterium, Pseudoxanthomonas sp. JBR18, for its potential as a plant growth-promoting agent with multiple beneficial properties. The strain exhibited tolerance to sodium chloride concentration of up to 7.5 % in the R2A medium. In vitro evaluation revealed that strain JBR18 possessed proteolytic, protease (EC 3.4), and cellulase (EC 3.2.1.4) activities, as well as the ability to produce indole-acetic acid, proline, and exopolysaccharides. Compared with the controls, co-cultivation of Arabidopsis seedlings with the strain JBR18 improved plant growth, rosette size, shoot and root fresh weight, and chlorophyll content under salinity stress. Moreover, JBR18-inoculated seedlings showed lower levels of malondialdehyde, reactive oxygen species, and Na+ uptake into plant cells under salt stress but higher levels of K+. Additionally, seedlings inoculated with JBR18 exhibited a delayed response time and quantity of salt-responsive genes RD29A , RD29B , RD20 , RD22 , and KIN1 under salt stress. These multiple effects suggest that Pseudoxanthomonas sp. JBR18 is a promising candidate for mitigating the negative impacts of salinity stress on plant growth. Our findings may assist in future efforts to develop eco-friendly strategies for managing abiotic stress and enhancing plant tolerance to salt stress. [Display omitted] • JBR18 significantly increased Arabidopsis biomass under salt and non-salt stress. • JBR18 inoculation stimulated indole-3-acetic acid production in Arabidopsis roots. • JBR18 decreased ROS leves and Na + ion uptake into plant cells under salt stress. • JBR18 reduced the salt stress response gene levels in Arabidopsis under salt stress. • JBR18 is a promising microbial agent that can mitigate the negative effects of salinity stress on plant growth. [ABSTRACT FROM AUTHOR]

Abstract: Trypanosoma brucei has a multifunctional RNA polymerase (pol) I that transcribes ribosomal gene units (RRNA) and units encoding its major cell surface proteins variant surface glycoprotein (VSG) and procyclin. Previous analysis of tandem affinity-purified, transcriptionally active RNA pol I identified ten subunits including an apparently trypanosomatid-specific protein termed RPA31. Another ortholog was identified in silico. No orthologs of the yeast subunit doublet RPA43/RPA14 have been identified yet. Instead, a recent report presented evidence that RPB7, the RNA pol II paralog of RPA43, is an RNA pol I subunit and essential for RRNA and VSG transcription in bloodstream form trypanosomes . Revisiting this attractive hypothesis, we were unable to detect a stable interaction between RPB7 and RNA pol I in either reciprocal co-immunoprecipitation or tandem affinity purification. Furthermore, immunodepletion of RPB7 from extract virtually abolished RNA pol II transcription in vitro but had no effect on RRNA or VSG ES promoter transcription in the same reactions. Accordingly, chromatin immunoprecipitation analysis revealed cross-linking of RPB7 to known RNA pol II transcription units but not to the VSG ES promoter or to the 18S rRNA coding region. Interestingly, RPB7 did crosslink to the RRNA promoter but so did the RNA pol II-specific subunit RPB9 suggesting that RNA pol II is recruited to this promoter. Overall, our data led to the conclusion that RNA pol I transcription in T. brucei does not require the RNA pol II subunit RPB7. [Copyright &y& Elsevier]