24 results on '"Li, Guoxi"'
Search Results
2. LncHLEF promotes hepatic lipid synthesis through miR-2188-3p/GATA6 axis and encoding peptides and enhances intramuscular fat deposition via exosome
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Guo, Yulong, Tian, Weihua, Wang, Dandan, Yang, Liyu, Wang, Zhang, Wu, Xing, Zhi, Yihao, Zhang, Ke, Wang, Yangyang, Li, Zhuanjian, Jiang, Ruirui, Sun, Guirong, Li, Guoxi, Tian, Yadong, Wang, Hongjun, Kang, Xiangtao, Liu, Xiaojun, and Li, Hong
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- 2023
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3. Nafion assisted preparation of prussian blue nanoparticles and its application in electrochemical analysis of l-ascorbic acid
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Li, Yuqiong, Pan, Fei, Yin, Shengyan, Tong, Chunyi, Zhu, Rilong, and Li, Guoxi
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- 2022
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4. Determination of manganese in submerged steel using Fraunhofer-type line generated by long-short double-pulse laser-induced breakdown spectroscopy
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Cui, Minchao, Deguchi, Yoshihiro, Li, Guoxi, Wang, Zhenzhen, Guo, Haorong, Qin, Zixiong, Yao, Changfeng, and Zhang, Dinghua
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- 2021
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5. Investigation of the correlation between the electrochemical noise energy and the deposit structure
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Liu, Jiao, Li, Guoxi, Zhu, Benfeng, Du, Xiaoqing, Yang, Yumeng, and Zhang, Zhao
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- 2021
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6. Calculation method for fractal characteristics of machining topography surface based on wavelet transform
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Li, Guoxi, Zhang, Kai, Gong, Jingzhong, and Jin, Xin
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- 2019
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7. Research on Assembly Data Mining Technology of Complex Mechanical System
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Zhang, Kai, Li, Guoxi, Gong, JingZhong, and Zhang, Meng
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- 2016
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8. Fabrication of polyaniline–silver nanocomposites by chronopotentiometry in different ionic liquid microemulsion systems
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Zhou, Zhou, He, Deliang, Guo, Yanni, Cui, Zhengdan, Wang, Minghao, Li, Guoxi, and Yang, Ronghua
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- 2009
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9. Mechanical properties of the composite lattice structure with variable density and multi-configuration.
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Zhang, Meng, Zhao, Cun, Li, Guoxi, and Luo, Kai
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COMPOSITE structures , *IMPACT loads , *MECHANICAL behavior of materials , *SELECTIVE laser sintering , *IMPACT testing , *STRENGTH of materials - Abstract
• A new composite lattice structure with variable density and multi-configuration arrangement. • Lattice cells with different configurations are arranged in different regions. • The thin rods of cells were improved by cell replacement. • The composite arrangement further enhances the mechanical properties of lattice materials. A new composite lattice structure is proposed by combining variable density and multi-configuration arrangement. The parameter mapping model is established, and three composite lattice structure models are presented. The samples are prepared by the additive manufacturing technology SLS (Selective Laser Sintering) with Nylon PA6. The samples are tested by the universal testing machine and drop weight impact testing system. The load-deformation, equivalent impact load and failure mode are analyzed. The results show that compared with the variable density lattice structure, composite model 1 (GBCC cells in the low-density region are replaced by ECC cells) improves the thin rods in the low-density region, composite model 2 (the cell sizes in the high-density region are increased while GBCC cells in the low-density region are replaced by ECC cells) increases the equivalent bending stiffness by 8%, enhances the bending and impact deformation resistance of materials, and composite model 3 (GBCC cells in the high-density region are replaced by BCCxy cells) increases the failure load by 77%, reduces the equivalent impact load by 47%, and improves the toughness and cushioning performance of materials. This work finds that the composite lattice structure with variable density and multi-configuration further improves the mechanical property of lattice materials. [ABSTRACT FROM AUTHOR]
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- 2023
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10. Simvastatin enhances NMDA receptor GluN2B expression and phosphorylation of GluN2B and GluN2A through increased histone acetylation and Src signaling in hippocampal CA1 neurons.
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Chen, Tingting, Zhang, Baofeng, Li, Guoxi, Chen, Lei, and Chen, Ling
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SIMVASTATIN , *METHYL aspartate receptors , *HISTONE acetylation , *ALZHEIMER'S disease , *FARNESOL - Abstract
Simvastatin (SV) can improve cognitive deficits in Alzheimer's disease patients and mice. Herein, we report that the administration of SV (20 mg/kg) for 5 days in mice (SV-mice) or the treatment of slices with SV (10 μM) for 4 h (SV-slices) could increase the density of NMDA-evoked inward currents ( I NMDA ) in hippocampal CA1 pyramidal cells, which were blocked by farnesol (FOH) that converts farnesyl pyrophosphate (FPP), but not geranylgeraniol (GGOH) that increases geranylgeranylpyrophosphate (GGPP). Sensitivity of I NMDA to ifenprodil in SV-mice or SV-slices was significantly increased. The levels of hippocampal GluN2B and GluN2A or Src phosphorylation in SV-mice or SV-slices were higher than controls, which were sensitive to FOH. The Src inhibitor PP2 could inhibit the SV-enhanced phosphorylation of GluN2B and GluN2A and SV-augmented I NMDA , but PI3K inhibitor LY294002 did not. The levels of GluN2B mRNA and protein were elevated in SV-mice, which was abolished by FOH, but not by GGOH or PP2. Furthermore, the histone H3K9 and H3K27 acetylation of GluN2B promoter was increased in SV-mice, which was suppressed by FOH rather than GGOH or PP2. In control mice and slices, the reduction of FPP by farnesyl transferase inhibitor could increase the levels of GluN2B expression, the histone H3K9 and H3K27 acetylation and enhance the phosphorylation of GluN2B, GluN2A and Src. The findings indicate that the administration of SV can enhance GluN2B expression and GluN2B and GluN2A phosphorylation leading to augmentation of NMDAR activity through reducing FPP to increase histone acetylation of GluN2B and Src signaling. [ABSTRACT FROM AUTHOR]
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- 2016
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11. Simvastatin prevents β-amyloid25–35-impaired neurogenesis in hippocampal dentate gyrus through α7nAChR-dependent cascading PI3K-Akt and increasing BDNF via reduction of farnesyl pyrophosphate.
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Wang, Conghui, Chen, Tingting, Li, Guoxi, Zhou, Libin, Sha, Sha, and Chen, Ling
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SIMVASTATIN , *ALZHEIMER'S disease treatment , *DEVELOPMENTAL neurobiology , *COGNITION disorders in children , *FARNESYL compounds , *PYROPHOSPHATES , *THERAPEUTICS - Abstract
Simvastatin (SV) is reported to improve cognition and slow progression of Alzheimer's disease (AD), however underlying mechanism still remains unclear. In hippocampal dentate gyrus (DG), β-amyloid (Aβ) selectively impairs survival and neurite growth of newborn neurons in the 2 nd week after birth. The aim of this study was to examine the effects of SV on the impairment of neurogenesis and the spatial cognitive deficits in Aβ 25–35 (3 nmol)-injected (i.c.v.) mice (Aβ 25–35 -mice). Herein, we reported that the SV-treatment (20 mg/kg) on days 2–14 after BrdU-injection could dose-dependently protect the survival and neurite growth of newborn neurons, which was blocked by the α7nAChR antagonist MLA or the farnesol (FOH) that can convert to farnesyl pyrophosphate (FPP), but not the α4β2nAChR antagonist DHβE. The SV-treatment in Aβ 25–35 -mice rescued the decline of Akt phosphorylation and increased the ERK1/2 phosphorylation in hippocampus, which was sensitive to MLA and FOH. The PI3K inhibitor LY294002 could abolish the SV-protected neurogenesis in Aβ 25–35 -mice, but the MEK inhibitor U0126 had no effects. The SV-treatment could correct the decline of hippocampal BDNF concentration in Aβ 25–35 -mice, which was blocked by MLA and FOH. Using Morris water maze and Y-maze tasks, we further observed that the SV-treatment in Aβ 25–35 -mice could improve their spatial cognitive deficits, which was sensitive to the application of FOH. The results indicate that the SV-treatment in Aβ 25–35 -mice via reduction of FPP can protect neurogenesis through α7nAChR-cascading PI3K-Akt and increasing BDNF, which may improve spatial cognitive function. [ABSTRACT FROM AUTHOR]
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- 2015
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12. Estrogen promotes gonadotropin-releasing hormone expression by regulating tachykinin 3 and prodynorphin systems in chicken.
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Wu, Xing, Zhang, Zihao, Li, Yijie, Zhao, Yudian, Ren, Yangguang, Tian, Yixiang, Hou, Meng, Guo, Yulong, Li, Qi, Tian, Weihua, Jiang, Ruirui, Zhang, Yanhua, Gong, Yujie, Li, Hong, Li, Guoxi, Liu, Xiaojun, Kang, Xiangtao, Li, Donghua, and Tian, Yadong
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GENE expression , *GONADOTROPIN releasing hormone , *CHICKENS , *ESTROGEN receptors , *KISSPEPTINS , *PRECOCIOUS puberty - Abstract
The "KNDy neurons" located in the hypothalamic arcuate nucleus (ARC) of mammals are known to co-express kisspeptin, neurokinin B (NKB), and dynorphin (DYN), and have been identified as key mediators of the feedback regulation of steroid hormones on gonadotropin-releasing hormone (GnRH). However, in birds, the genes encoding kisspeptin and its receptor GPR54 are genomic lost, leaving unclear mechanisms for feedback regulation of GnRH by steroid hormones. Here, the genes tachykinin 3 ( TAC3 ) and prodynorphin ( PDYN ) encoding chicken NKB and DYN neuropeptides were successfully cloned. Temporal expression profiling indicated that TAC3, PDYN and their receptor genes (TACR3, OPRK1) were mainly expressed in the hypothalamus, with significantly higher expression at 30W than at 15W. Furthermore, overexpression or interference of TAC3 and PDYN can regulate the GnRH mRNA expression. In addition, in vivo and in vitro assays showed that estrogen (E2) could promote the mRNA expression of TAC3, PDYN , and GnRH , as well as the secretion of GnRH/LH. Mechanistically, E2 could dimerize the nuclear estrogen receptor 1 (ESR1) to regulate the expression of TAC3 and PDYN , which promoted the mRNA and protein expression of GnRH gene as well as the secretion of GnRH. In conclusion, these results revealed that E2 could regulate the GnRH expression through TAC3 and PDYN systems, providing novel insights for reproductive regulation in chickens. [ABSTRACT FROM AUTHOR]
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- 2024
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13. Adiponectin inhibits GnRH secretion via activating AMPK and PI3K signaling pathways in chicken hypothalamic neuron cells.
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Wu, Xing, Tao, Yiqing, Ren, Yangguang, Zhang, Zihao, Zhao, Yudian, Tian, Yixiang, Li, Yijie, Hou, Meng, Guo, Yulong, Gong, Yujie, Zhang, Yanhua, Li, Donghua, Li, Hong, Jiang, Ruirui, Li, Guoxi, Liu, Xiaojun, Kang, Xiangtao, and Tian, Yadong
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AMP-activated protein kinases , *GONADOTROPIN releasing hormone , *HYPOTHALAMUS , *HYPOTHALAMIC hormones , *CHICKENS , *PHOSPHATIDYLINOSITOL 3-kinases - Abstract
It has been reported that adiponectin (AdipoQ), an adipokine secreted by white adipose tissue, plays an important role in the control of animal reproduction in addition to its function in energy homeostasis by binding to its receptors AdipoR1/2. However, the molecular mechanisms of AdipoQ in the regulation of animal reproduction remain elusive. In this study, we investigated the effects of AdipoQ on hypothalamic reproductive hormone (GnRH) secretion and reproduction-related receptor gene (estrogen receptor [ ER ] and progesterone receptor [ PR ]) expression in hypothalamic neuronal cells (HNCs) of chickens by using real-time fluorescent quantitative PCR (RT-qPCR), enzyme-linked immunosorbent assay (ELISA), Western blot (WB) and cell counting kit-8 (CCK-8) assays and found that overexpression of AdipoQ could increase the expression levels of AdipoR1/2 and reproduction-related receptor genes (P < 0.05) while decreasing the expression level of GnRH. In contrast, interference with AdipoQ mRNA showed the opposite results in HNCs. Furthermore, we demonstrated that AdipoQ exerts its functions through the AMPK and PI3K signaling pathways. Finally, our in vitro experiments found that AdipoRon (a synthetic substitute for AdipoQ) treatment and AdipoR1/2 RNAi interference co-treatment resulted in no effect on GnRH secretion, suggesting that the inhibition of GnRH secretion by AdipoQ is mediated by the AdipoR1/2 signaling axis. In summary, we uncovered, for the first time, the molecular mechanism of AdipoQ in the regulation of reproductive hormone secretion in hypothalamic neurons in chickens. [ABSTRACT FROM AUTHOR]
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- 2023
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14. Effect of solvents on the preparation and corrosion protection of polypyrrole.
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Yan, Qingyu, Pan, Wenyan, Zhong, Shasha, Zhu, Rilong, and Li, Guoxi
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POLYPYRROLE , *CORROSION & anti-corrosives , *LEWIS basicity , *SOLVENTS , *SCANNING electron microscopy , *STAINLESS steel - Abstract
• The DN value of solvent has effect on PPy's preparation. • The lower the DN value, the less structural defects of PPy. • The lower the DN value, the higher conductivity of PPy. • PPy prepared in nitromethane solutions own the best corrosion protection performances. To clarify the effect of solvents with different donor number (DN) on the preparation and corrosion protection ability of polypyrrole (PPy) films, PPy were firstly synthesized in water or acetonitrile or nitromethane solutions containing NaClO 4 + pyrrole by a potentiostatic method. The corrosion protection performances of these films with stainless steel as a substrate were investigated by open circuit potential, potentiodynamic polarization and electrochemical impedance spectroscopy in 1 M H 2 SO 4. Results indicated that all PPy films showed spherical structure in scanning electron microscopy photograph and corrosion protection ability. Particularly, PPy prepared in nitromethane solutions performed the best due to the lowest DN value. Nitromethane had the weakest interaction with the intermediates of pyrrole polymerization which results in the smallest particles, the most compact accumulation, the highest conductivity and the longest conjugated chain. [ABSTRACT FROM AUTHOR]
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- 2019
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15. Chicken ZNF764L gene: mRNA expression profile, alternative splicing analysis and association analysis between first exon indel mutation and economic traits.
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Han, Ruili, Wang, Xiangnan, Wang, Xinlei, Guo, Yaping, Li, Donghua, Li, Guoxi, Wang, Yanbin, Kang, Xiangtao, and Li, Zhuanjian
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CHICKENS , *MESSENGER RNA , *GENE expression , *RNA splicing , *GENETIC mutation , *EXONS (Genetics) - Abstract
Abstract Zinc finger proteins are a class of transcription factors with finger-like domains and have diverse uses in biological processes, including development, differentiation, and metabolism. In this study, we identified the absence of the 24 bp sequence in the third exon of the zinc finger protein 764-like (ZNF764L) gene that lead to the production of two new transcripts, ZNF764L-SV1 and ZNF764L-SV2 , and the sum of the expression levels of the two transcripts is approximately equal the total RNA expression level. Temporal and spatial expression showed that ZNF764L had higher expression during the embryonic stage. Moreover, the research study revealed a 22-bp indel mutation in the first exon region of ZNF764L gene. Statistically significant results (P < 0.05) were encountered for this indel for chicken growth and carcass traits, which include birth weight, chest breadth and body slanting length at 4 weeks of age and subcutaneous fat weight and others. Genetic parameter analysis showed that D is the predominant allele in the commercial chicken population. Gene expression for each genotype showed that birds carrying the II allele had a higher expression level than the other genotypes. These findings enrich the understanding of ZNF764L gene function and enhance reproduction in the chicken industry. Highlights • The absence of the third exon of the ZNF764L gene led to two new transcripts. • The ZNF764L have higher expression during embryonic stage. • A new 22-bp insertion variant sequence on the first exon of the ZNF764L gene contains a stop codon (TAG). [ABSTRACT FROM AUTHOR]
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- 2019
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16. multiallelic indel in the promoter region of the Cyclin-dependent kinase inhibitor 3 gene is significantly associated with body weight and carcass traits in chickens.
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Li, Wenya, Liu, Danli, Tang, Shuqi, Li, Donghua, Han, Ruili, Tian, Yadong, Li, Hong, Li, Guoxi, Li, Wenting, Liu, Xiaojun, Kang, Xiangtao, and Li, Zhuanjian
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ANIMAL carcasses , *KINASE inhibitors , *CHICKEN breeds , *BODY weight , *LIVESTOCK growth - Abstract
Many studies have reported that cyclin-dependent kinase inhibitor 3 (CDKN3) is involved in the cell cycle. However, the function of CDKN3 has not been well elucidated in organisms. In this study, a multiallelic indel caused by a 19-bp fragment and a 2 × 19 bp fragment was shown for the first time to be inserted into the promoter of the CDKN3 gene in 1994 chickens from 9 different breeds. In addition, 6 genotypes (C5C5, C4C4, C3C3, C4C5, C3C4, and C3C5) were observed (C3C3, C4C4, C5C5 have 3 × 19 bp, 4 × 19 bp, and 5 × 19 bp, respectively). Among these genotypes, the C4C4 genotype was the most dominant genotype in 9 breeds. The results of χ2 analysis of CDKN3 gene in different breeds showed that there were significant differences in the distribution of genotypes among different cultivars (P < 0.01). In addition, association study with F2 chicken resource population which produced by Anka and Gushi chickens showed that the C3C4 genotypes had the greatest semi-evisceration weight (SEW, 1163.94 ± 46.84), evisceration weight (EW, 964.15 ± 41.16), head weight (HW, 45.55 ± 1.43), claw weight (CW, 63.42±2.86), wing weight (WW, 129.15±5.48), liver weight (LW, 29.96±1.27), carcass weight (cW, 1286.96±49.53), weight at 10 (1190.68±45.68) and 12 (1430.65±54.45) wk, followed by C3C3, C4C4, C5C5, C4C5, whereas C3C5 genotypes having the lowest SEW (989.21±47.71), EW (841.38±40.55), HW (41.03±1.46), CW (54.36±2.81), WW (116.31±5.39), LW (27.31±1.25), cW (1093.29±49.99), weight at 10 (1036.10±44.99) and 12 (1246.28±53.59) wk. Expression levels of CDKN3 in breast muscle of chickens with C4C4 (0.72±0.02), C3C3 (0.95±0.41), and C4C5 (0.74±0.13) genotypes were significantly lower than those with C5C5 (1.80±0.01) and C3C5 (2.14±0.17) genotypes (P < 0.05). In conclusion, we investigated the effect of a multiallelic indel in the CDKN3 gene on the economic traits of chickens, and this indel was significantly associated with growth and carcass traits in chickens. Collectively, our findings provide useful information about the repeat sequence indel in the promoter region of the CDKN3 gene as a potential molecular marker for chicken breeding. [ABSTRACT FROM AUTHOR]
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- 2019
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17. Carcass composition, meat quality, leg muscle status, and its mRNA expression profile in broilers affected by valgus-varus deformity.
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Cai, Chunxia, Zhang, Lujie, Liu, Xinxin, Li, Jianzeng, Ma, Yanchao, Jiang, Ruirui, Li, Zhuanjian, Li, Guoxi, Tian, Yadong, Kang, Xiangtao, and Han, Ruili
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LEG muscles , *GENE expression , *MEAT quality , *MOLECULAR biology , *ERECTOR spinae muscles , *COLOR of meat - Abstract
Valgus-varus deformity (VVD) is a common leg disease in commercial broilers, which seriously affects animal welfare and causes economic losses. Up to now, most of the studies on VVD have been on skeleton, whereas there are fewer studies on VVD muscle. In this study, carcass composition and meat quality of 35-day-old normal and VVD Cobb broilers assess the effect of VVD on broiler growth. Molecular biology, morphology, and RNA sequencing (RNA-seq) were used to study the difference between normal and VVD gastrocnemius muscle. In comparison with the normal broilers, the breast muscle and leg muscle of the VVD broilers had lower shear force, notably lower crude protein, lower water content, cooking loss, and deeper meat color (P < 0.05). The morphological results showed that the weight of skeletal muscle was significantly higher in the normal broilers than that in the VVD broilers (P < 0.01), the diameter and area of myofibrils in the affected VVD were smaller than in the normal broilers (P < 0.01). Quantitative real-time PCR (qPCR) of gastrocnemius muscle revealed that the expression of myasthenic marker genes, fast myofiber marker genes, and apoptosis-related factors were significantly higher in the VVD broilers than in the normal broilers (P < 0.01). In total, 736 differentially expressed genes (DEGs) were identified firstly in the normal and VVD leg muscle by RNA-seq. Gene ontology (GO) enrichment indicated that these DEGs were mainly involved in the multicellular organismal process and anatomical structure development. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that DEGs are significantly enriched in proteasome. Protein interaction analysis obtained that DEGs with high interaction were proteasome-related coding genes and ubiquitin-related genes, these DEGs were closely associated with muscle atrophy. These show that VVD has an adverse effect on growth characteristics, slaughter characteristics, and meat quality in broilers, which may cause leg muscle atrophy. This study provides some reference values and basis for studying the pathogenesis of VVD in broilers. [ABSTRACT FROM AUTHOR]
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- 2023
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18. Research Note: Combined analysis of BSA-seq based mapping and RNA-seq reveals candidate genes associated with sub-Columbian plumage in H line chickens.
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Li, Ruiting, Wang, Xinlei, Wang, Yanxing, liu, Danli, Zhang, Yushi, Liu, Yang, Niu, Xinran, Han, Ruili, Li, Hong, Jiang, Ruirui, Sun, Guirong, Li, Guoxi, Tian, Yadong, Liu, Xiaojun, Kang, Xiangtao, and Li, Zhuanjian
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COLOR of birds , *POULTRY breeding , *FEATHERS , *CYCLIN-dependent kinase inhibitors , *RNA sequencing , *CHICKENS , *HAPLOTYPES - Abstract
Columbian coloration patterns in plumage are widespread phenomena in several standard breeds of poultry, such as the Columbian Plymouth Rock chicken. H line chicken plumage is generally a pure white except in the hackle, wing, and tail plumage, which coloration is very similar to the Columbian plumage pattern, but with the barring substituting for the black vertical striping. Thus, we refer to this plumage coloration as "sub-Columbian" pattern. However, the genetic basis of this phenotype remains unknown. Here, a F3 cross population between yellow plumage roosters and sub-Columbian plumage hens was constructed, for verifying sub-Columbian plumage was sex-linked dominant inheritance. To identify the candidate regions, F2 generation sub-Columbian plumage hens and yellow plumage hens with their parental lines were used for BSA-seq, and sub-Columbian plumage genes were mapped to a 10.46 Mb interval on chromosome Z. Remarkably, by transcriptome analysis of the neck and wing tip follicle tissues of the 2 plumage colors, we demonstrated that within the interval, only 1 gene, SLC45A2 expressed significant differently (P < 0.05). Through KASP, we identified L347M and A10331272T in solute carrier family 45 member 2 ( SLC45A2 ), and B2 haplotype of cyclin-dependent kinase inhibitor 2A ( CDKN2A ), showed near complete association with the phenotype. Eventually, we designed a hybridization experiment for verifying the locus of sub-Columbian plumage, which is inherited through Z-linked dominant inheritance and is controlled by SLC45A2 and CDKN2A. [ABSTRACT FROM AUTHOR]
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- 2023
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19. The adiponectin receptor agonist, AdipoRon, promotes reproductive hormone secretion and gonadal development via the hypothalamic-pituitary-gonadal axis in chickens.
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Li, Chong, Cao, Yanfang, Ren, Yangguang, Zhao, Yudian, Wu, Xing, Si, Sujin, Li, Jing, Li, Qi, Zhang, Na, Li, Donghua, Li, Guoxi, Liu, Xiaojun, Kang, Xiangtao, Jiang, Ruirui, and Tian, Yadong
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HYPOTHALAMIC-pituitary-gonadal axis , *ADIPONECTIN , *SECRETION , *ADIPOSE tissues , *HENS , *PRECOCIOUS puberty - Abstract
Adiponectin is a key hormone secreted by fat tissues that has multiple biological functions, including regulating the energy balance and reproductive system by binding to its receptors AdipoR1 and AdipoR2. This study investigated the correlation between the levels of adiponectin and reproductive hormones in the hypothalamic-pituitary-ovarian (HPO) axis of laying hens at 4 different developmental stages (15, 20, 30, and 68 wk) and explored the effects of AdipoRon (an activator of adiponectin receptors) on the hypothalamic-pituitary-gonadal (HPG) axis and follicle and testicular Leydig cells in vitro and in vivo. The results demonstrated that the adiponectin level was significantly correlated with that of reproductive hormones in the HPO axis (e.g., GnRH, FSH, LH, and E2) in laying hens at 4 different ages. Moreover, AdipoRon could promote the expression of AdipoR1 and AdipoR2 and the secretion of reproductive hormones in the HPG axis, including GnRH, FSH, LH, P4, and T. AdipoRon could also upregulate the expression of genes related to follicular steroidogenesis (STAR, CYP19A1, CYP17A1, and CYP11A1), hepatic lipid synthesis (OVR, MTP), follicular lipid uptake (PPAR-g), and follicular angiogenesis (VEGFA1, VEGFA2, VEGFR1, ANGPT1, ANGPT2, TEK) in the oviposition period, and all of these findings were consistent with the results obtained from in vitro experiments after the transfection of small white follicles (SWFs) with AdipoRon. Furthermore, the results suggest that AdipoRon increases the diameter of testicular seminiferous tubules, the number of spermatogenic cells and sperm production in vivo and enhances the expression of AdipoR1, AdipoR2 and steroid hormones in vitro. Collectively, the findings suggest that AdipoRon could facilitate the expression and secretion of reproductive hormones in the HPG axis by activating its receptors and then improve the growth and development of follicles and testes in chickens. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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20. GPNMB promotes abdominal fat deposition in chickens: genetic variation, expressional profile, biological function, and transcriptional regulation.
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Wang, Dandan, Teng, Menglin, Wang, Yangyang, Cao, Yuzhu, Tian, Weihua, Wang, Zhang, Guo, Yulong, Li, Hong, Li, Zhuanjian, Jiang, Ruirui, Li, Guoxi, Tian, Yadong, and Liu, Xiaojun
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ABDOMINAL adipose tissue , *ESTROGEN receptors , *GENETIC variation , *GENETIC transcription regulation , *GENE expression , *PEROXISOME proliferator-activated receptors , *ADIPOSE tissues - Abstract
Glycoprotein nonmetastatic melanoma protein B (GPNMB) is a vital secreted factor that promotes the occurrence of obesity in mammals. However, the effects of GPNMB on abdominal fat deposition is still unknown in chickens. In this study, we looked into the genetic and expression association of GPNMB gene with abdominal fat traits in chicken, and found that a genetic variation rs31126482 in GPNMB promoter was significantly associated with abdominal fat weight (AFW , P < 0.05) and abdominal fat percentage (AFP , P < 0.01). Express profile analysis of the GPNMB indicated that the gene was mainly expressed in abdominal fat tissue, and its expression level was strongly positively correlated with AFW (R2 = 0.6356, P = 4.10E−05) and AFP (R2 = 0.6450, P = 2.90E−05). We then investigated biological function of GPNMB on adipogenesis in chicken, and found that GPNMB could inhibit abdominal preadipocyte proliferation, but promote abdominal preadipocyte differentiation and lipid deposition. Furthermore, we explored regulatory mechanism of GPNMB gene in chicken, and detected one nonclassical estrogen regulatory element (AP1) and one peroxisome proliferator-activated receptor α (PPARα) responsive element in the 2 kb promoter region of GPNMB gene, and demonstrated that estrogen could up-regulate GPNMB mRNA expression in adipose tissue and primary abdominal preadipocytes, while PPARα could down-regulate GPNMB expression in primary preadipocytes. Taken together, this study brings new insights into understanding the function and transcriptional control of GPNMB gene, and provides genetic markers for breeding selection to improve abdominal fat traits in chicken. [ABSTRACT FROM AUTHOR]
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- 2022
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21. Effect of polymorphism within miRNA-1606 gene on growth and carcass traits in chicken.
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Li, Hong, Wang, Shanhe, Yan, Fengbin, Liu, Xiaojun, Jiang, Ruirui, Han, Ruili, Li, Zhuanjian, Li, Guoxi, Tian, Yadong, Kang, Xiangtao, and Sun, Guirong
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SINGLE nucleotide polymorphisms , *MICRORNA , *ANIMAL genetics , *CHICKENS , *PHENOTYPES , *MESSENGER RNA , *BODY weight - Abstract
Genetic variations in microRNAs (miRNAs) including primary miRNAs, precursor miRNAs and mature miRNAs can lead to phenotypic variation by altering the biogenesis of miRNAs and/or their binding to target mRNAs. Increasing functional studies suggest that polymorphisms occurring in miRNAs can lead to phenotypic variation in farm animal. Here, we identified a single nucleotide polymorphism (SNP) located in the precursor of chicken miRNA-1606 gene. The association study on body indexes, body weight at different growth stages, and carcass traits was performed in a Gushi–Anka F 2 population resource. The SNP was not only significantly associated with body weight at 10 and 12 weeks, respectively, but also with chicken shank length, chest depth and body slanting length at 8 weeks; shank length, pectoral angle, body slanting length and pelvis breadth at 12 weeks, respectively. And the polymorphism was also significantly associated with carcass traits including semi-evisceration weight, evisceration weight, breast muscle weight, leg weight and carcass weight as well. The observed values of individuals with CA genotype were significantly higher than CC genotype both in body weight at different stages and carcass traits. This SNP altered the predicted second structure of pre-mir-1606, with the altering of the free energy values. And the relative expression level of mature miRNA between CA and AA was significantly changed in leg muscle. Our data suggested that miRNA-1606 may be a candidate gene associated with chicken growth traits. [ABSTRACT FROM AUTHOR]
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- 2015
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22. MiR-29b-1-5p regulates the proliferation and differentiation of chicken primary myoblasts and analysis of its effective targets.
- Author
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Li, Yuanfang, Zhai, Bin, Yuan, Pengtao, Fan, Shengxin, Jin, Wenjiao, Li, Wenting, Sun, Guirong, Tian, Yadong, Liu, Xiaojun, Kang, Xiangtao, and Li, Guoxi
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MUSCLE growth , *STRIATED muscle , *CHICKENS , *LACTATE dehydrogenase , *MORPHOGENESIS , *MYOBLASTS - Abstract
Several recent studies investigated the role of the miR-29 family in muscle development. However, only a few studies focused on chicken skeletal muscle. In the present study, cell cycle, 5-ethynyl-2'-deoxyuridine (EdU), cell counting kit-8 (CCK-8), and other assays indicated that miR-29b-1-5p can inhibit the proliferation of chicken primary myoblasts (CPMs); the western blot assay and immunofluorescence detection of MYHC demonstrated that miR-29b-1-5p can promote the differentiation of myoblasts. The functional enrichment analysis revealed that the target genes of miR-29b-1-5p may be involved in muscle tissue development, muscle organ development, and striated muscle tissue development, which are biological processes related to muscle development. The correlation analysis showed that these 6 genes, that is, ankyrin repeat domain 9 ( ANKRD9 ), lactate dehydrogenase A ( LDHA ), transcription factor 12 ( TCF12 ), FAT atypical cadherin 1 ( FAT1 ), lin-9 homolog ( LIN9 ), and integrin beta 3 binding protein ( ITGB3BP ), can be used as effective candidate target genes of miR-29b-1-5p. Moreover, miR-29b-1-5p inhibits the expression of ANKRD9 by directly binding the 3′UTR of ANKRD9. Overall, these data indicate that miR-29b-1-5p inhibits the proliferation of primary chicken myoblasts, stimulates their differentiation, and is involved in the process of muscle development and that its effective target gene is ANKRD9. This study identified the molecular mechanism of miR-29b-1-5p in chicken muscle development. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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23. Comparative analysis of hypothalamus transcriptome between laying hens with different egg-laying rates.
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Ma, Zheng, Jiang, Keren, Wang, Dandan, Wang, Zhang, Gu, Zhenzhen, Li, Guoxi, Jiang, Ruirui, Tian, Yadong, Kang, Xiangtao, Li, Hong, and Liu, Xiaojun
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HENS , *POULTRY breeding , *TRANSCRIPTOMES , *CELLULAR signal transduction , *CHICKEN breeds , *AGRICULTURAL egg production , *HYPOTHALAMUS , *EGGS - Abstract
Egg-laying performance is one of the most important economic traits in the poultry industry. Commercial layers can lay one egg almost every day during their peak-laying period. However, many Chinese indigenous chicken breeds show a relatively low egg-laying rate, even during their peak-laying period. To understand what makes the difference in egg production, we compared the hypothalamus transcriptome profiles of Lushi blue-shelled-egg chickens (LBS), a Chinese indigenous breed with low egg-laying rate and Rhode Island Red chickens (RIR), a commercial layer with relatively high egg-laying rate using RNA-seq. A total of 753 differentially expressed genes (DEGs) were obtained. Of these DEGs, 38 genes were enriched in 2 Gene Ontology (GO) terms, namely reproduction term and the reproductive process term, and 6 KEGG pathways, namely Wnt signaling pathway, Oocyte meiosis, GnRH signaling pathway, Thyroid hormone signaling pathway, Thyroid hormone synthesis and MAPK signaling pathway, which have been long known to be involved in egg production regulation. To further determine the core genes from the 38 DEGs, protein-protein interaction (PPI) network, co-expression network and transcriptional regulatory network analyses were carried out. After integrated analysis and experimental validation, 4 core genes including RAC1, MRE11A, MAP7 and SOX5 were identified as the potential core genes that are responsible for the laying-rate difference between the 2 breeds. These findings paved the way for future investigating the mechanism of egg-laying regulation and enriched the chicken reproductive regulation theory. [ABSTRACT FROM AUTHOR]
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- 2021
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24. Molecular characterization and a duplicated 31-bp indel within the LDB2 gene and its associations with production performance in chickens.
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Wei, Chengjie, Hou, Dan, Feng, Yi, Li, Tong, Jing, Zhenzhu, Li, Wenya, Han, Ruili, Li, Guoxi, Sun, Guirong, Tian, Yadong, Liu, Xiaojun, Kang, Xiangtao, and Li, Zhuanjian
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LEG muscles , *REGULATOR genes , *CHICKEN breeds , *CHICKENS , *GENE expression , *ERECTOR spinae muscles , *PECTORALIS muscle , *ANIMAL carcasses - Abstract
• A 31-bp indel located in LDB2 gene was detected in chicken for the first time. • The indel cause significant differences in growth and carcass traits of F2. • The expression of LDB2 gene in breast muscle was higher than that in other tissues. • In the breast muscle, it reached the highest at the age of 6 weeks. • The 31-bp indel can be used as a genetic marker in chicken molecular breeding. Many studies have shown that the LDB2 gene plays a regulatory role in retinal development and the cell cycle, but its biological role remains unclear. In this study, a 31-bp indel in the LDB2 gene was found for the first time on the basis of 2797 individuals from 10 different breeds, which led to different genotypes among individuals (II, ID and DD). Among these genotypes, DD was the most dominant. Association analysis of an F 2 resource population crossed with the Gushi (GS) chicken and Anka chicken showed that the DD genotype conferred a significantly greater semi-evisceration weight (SEW, 1108.665 g ± 6.263), evisceration weight (EW, 927.455 g ± 5.424), carcass weight (CW, 1197.306 g ± 6.443), breast muscle weight (BMW, 71.05 g ± 0.574), and leg muscle weight (LMW, 100.303 g ± 0.677) than the ID genotype (SEW, 1059.079 g ± 16.86; EW, 879.459 g ± 14.446; CW, 1141.821 g ± 17.176; BMW, 67.164 g ± 1.523; and LMW, 96.163 g ± 1.823). In addition, LDB2 gene expression in different breeds was significantly higher in the breast muscles and leg muscles than in other tissues. The expression level in the breast muscle differed significantly among stages of GS chicken development, with the highest expression observed at 6 weeks. The expression levels in the pectoral muscles differed significantly among Ross 308 genotypes. In summary, we studied the relationships between a 31-bp indel in the LDB2 gene and economic traits in chickens. The indel was significantly correlated with multiple growth and carcass traits in the F 2 resource population and affected the expression of the LDB2 gene in muscle tissue. In short, our study revealed that the LDB2 gene 31-bp indel can be used as a potential genetic marker for molecular breeding. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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