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• Cleaning methods for RO membranes were optimized for full-scale applications. • The optimized cleaning method removes more inorganic elements and organic matter. • NaCl can disrupt the complex structure of organic matter by ion exchange. • The optimized cleaning method can change the microbial community structure. • The optimized cleaning method is 1.65 times more efficient than regular methods. Membrane fouling has always been a critical constraint in the operation of the reverse osmosis (RO) process, and chemical cleaning is essential for mitigating membrane fouling and ensuring smooth operation of the membrane system. This paper presents an optimized chemical cleaning method for the efficient cleaning of RO membranes in full-scale applications. Compared to the regular cleaning method (cleaning with 0.1 % NaOH + 1 % ethylenediaminetetraacetic acid + 0.025 % sodium dodecyl benzene sulfonate followed by 0.2 % HCl), the optimized cleaning method improves the cleaning efficiency by adding sodium chloride to the alkaline cleaning solution and citric acid to the acid cleaning solution. Notably, the membrane flux recovery rate with the optimized cleaning method is 45.74 %, and it improves the cleaning efficiency by 1.65 times compared to the regular cleaning method. Additionally, the optimized cleaning method removes 30.46 % of total foulants (organic and inorganic), which is 2.11 times higher than the regular cleaning method. The removal of inorganic ions such as Fe, Ca, and Mg is significantly improved with the optimized cleaning method. For organic matter removal, the optimized cleaning method effectively removes more polysaccharides, proteins, and microbial metabolites by disrupting the complex structures of organic matter. Furthermore, it also changes the microbial community structure on the RO membrane surface by eliminating microorganisms that cannot withstand strong acids, bases, and high salt environments. However, Mycobacterium can adapt to these harsh conditions, showing a relative abundance of up to 84.13 % after cleaning. Overall, our results provide a new chemical cleaning method for RO membranes in full-scale applications. This method effectively removes membrane foulants and enhances the understanding of the removal characteristics of foulants on RO membrane surfaces by chemical cleaning. [ABSTRACT FROM AUTHOR]

[Display omitted] Histone acetylation is one of the most essential parts of epigenetic modification, mediating a variety of complex biological functions. In these procedure, p300/CBP could catalyze the acetylation of lysine 27 on histone 3 (H3K27ac), and had been reported to mediate tumorigenesis and development in a variety of tumors by enhancing chromatin transcription activity. Ovarian cancer, as an extremely malignant tumor, has also been observed to undergo abnormal acetylation of histones. However, whether the treatment of ovarian cancer could be achieved by inhibiting the acetylation activity of p300/CBP on H3K27 has not been well investigated. In this article, we modified the structure of p300/CBP HAT domain inhibitor A-485 and obtained a highly active small molecule known as 13f , which has an IC 50 value of 0.49 nM for inhibiting the in vitro enzyme activity of p300, as well as the anti-proliferation IC 50 value on ovarian cancer cell line OVCAR-3 was 153 nM. In addition, 13f had strong acetylase family selectivity, good metabolic stability and promising in vivo anti-tumor activity in OVCAR-3 xenograft model. The discovery of 13f revealed a more active chemical entity of the HATs domain of p300/CBP and provided a novel idea for the application of epigenetic inhibitors in the treatment of ovarian cancer. [ABSTRACT FROM AUTHOR]