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To observe the clinical therapeutic effect of laser acupuncture on type 2 diabetes mellitus (T2DM) of qi and yin deficiency type. Laser acupuncture was used to treat 32 patients of T2DM. Acupoint selection: Fèishū (肺俞BL13), Píshū (脾俞BL20), Shènshū (肾俞BL23), Wǎnxiàshū (胃脘下俞EX-B3), Sānyīnjiāo (三阴交SP6) and Zúsānlĭ 足三里(ST36). It took 20 min in each treatment, five treatments a week. There were 2 days of interval in 2 weeks of treatment. Before and after treatment, the changes in fasting plasma glucose (FPG), postprandial 2 h glucose (2hPG), fasting insulin (FINS), glycosylated albumin (GA), insulin resistance index (HOMA-IR) and the symptom score of traditional Chinese medicine (TCM) were observed in the patients. The therapeutic effect was evaluated. Of 32 cases, 8 cases were remarkably effective, 18 cases effective and 6 cases failed. The total effective rate was 81.3%. At the end of treatment course, the level of every indicator after treatment was significantly lower than that before treatment (all P < 0.01). Laser acupuncture achieves the significant therapeutic effect on T2DM of qi and yin deficiency and this therapy deserves to be promoted in clinical application. [ABSTRACT FROM AUTHOR]