1. Colocalization and identification of interaction sites between IGFBP-3 and GalNAc-T14
- Author
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Wu, Chen, Ma, Si-Si, Ge, Jian-Feng, Wang, Yuan-Yuan, Tian, Huan-Na, Liu, Xiao-Bo, Zhang, Bo, Liu, Fang-Ming, Zhang, Xiao-Kang, and Li, Qin-Jian
- Subjects
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INSULIN-like growth factor-binding proteins , *GLYCOSYLTRANSFERASES , *CONFOCAL microscopy , *CANCER cell culture , *ESCHERICHIA coli , *GLUTATHIONE transferase , *BREAST cancer - Abstract
Abstract: GalNAc-T14 was identified as a novel IGFBP-3 binding partner in previous studies. Here, we furtherly confirmed the interaction between them by confocal microscopy, and identified the binding domain and probable interaction sites of GalNAc-T14 with IGFBP-3. The result of subcellular localization indicated that GalNAc-T14 was distributed in the cytosol, whereas IGFBP-3 existed in the cytosol and nucleolus. Confocal analyses demonstrated that IGFBP-3 and GalNAc-T14 colocalized in the cytosol. The result from yeast two hybrid assay showed that the C terminus of GalNAc-T14 (408–552aa) was essential for the interaction between GalNAc-T14 and IGFBP-3, especially Tyr408, Pro409, and Glu410 of GalNAc-T14 may play key roles in the interaction with IGFBP-3. In conclusion, these studies demonstrated that IGFBP-3 and GalNAc-T14 are colocalized in MCF-7 cells and confirmed the interaction between IGFBP-3 and GalNAc-T14. This interaction may play an important role in the functional regulation of IGFBP-3. [Copyright &y& Elsevier]
- Published
- 2012
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