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As a result of increasingly stringent power supply requirements and diverse application scenarios, the development of hybrid nanogenerators that can combine multiple power generation modes has gradually become a hot research topic. In this work, the PR-HNG, a piezo/triboelectric hybrid nanogenerator based on a pitched roof structure, is proposed and is used efficiently to perform discrete mechanical energy harvesting. Using the flexible connection of its separated roof-like trigger mechanism, the PR-HNG operates through inertia under vibration conditions and achieves a satisfactory working state with very low mechanical loss. At 10 Hz, the instantaneous open-circuit voltage V OC and short-circuit current I SC of the PR-HNG can reach 800 V and 9.6 mA (1.60 A/m2), respectively, and no obvious attenuation occurs during high-intensity and long-term operation. After connection to a load via a rectifier bridge, the PR-HNG has a maximum power output of 575 μW/cm2 and can charge a 2 mF capacitor up to more than 2.5 V in only 30 s; these two performance parameters are better than the reported values for other similar types of HNG. In practical applications, this PR-HNG can easily drive more than 1440 commercial light-emitting diodes and can drive small electronic devices in real time. Therefore, the PR-HNG has been demonstrated to provide a credible and promising solution for fabrication of self-powered monitoring systems for use in industrial environments. [Display omitted] • A pitched roof-like HNG effectively inherits the performance of TENG and PENG. • The HNG also has credible output performance even at different inclinations. • The power density of HNG is up to 5.75 W/m2 and 145.3 W/m3. • The HNG has strong charging ability, which assists monitoring system in IIoT. [ABSTRACT FROM AUTHOR]

[Display omitted] • An HPLC-MS/MS method to detect the subunits of glycinin and β-conglycinin was developed using without protein standards. • Quantitative peptides of each subunit of glycinin and β-conglycinin were screened. • Stable isotope-labelled internal standard peptides were used for quantification. • Glycinin and β-conglycinin in processed soybean products were quantified. Glycinin and β-conglycinin are the two main allergic proteins in soybean. Due to their complex structures and lack of protein standards, it is difficult to achieve quantitative determination of these proteins in soybeans. In this study, an HPLC-MS/MS method was developed for the simultaneous determination of five subunits of glycinin (G1, G2, G3, G4, and G5) and three subunits of β-conglycinin (α, α', and β) in processed soybean products based on 8 specific peptides and their stable isotope-labeled peptides. Here, each specific peptide was derived from one of the above 8 subunits. When soy protein was extracted and digested with trypsin, 8 specific peptides, and corresponding stable isotope-labeled peptides were analyzed by HPLC-MS/MS. The linear range for the specific peptides was between 3.2 and 1000 ng/mL (R2 > 0.9955). The recoveries of added peptides ranged from 83.4% to 117.8%, and the intra-day precisions (% CV) were below 17.4%. The limit of quantification of each subunit of glycinin and β-conglycinin in processed soybean products (in terms of protein amount) was between 15.1 and 156.1 g/g. This method was successfully applied to the analysis of 8 subunits of glycinin and β-conglycinin in 68 different processed soybean products, which provides technical support for processed product quality evaluation and monitoring soybean processing technology. [ABSTRACT FROM AUTHOR]

In postconfluent human pulmonary microvascular endothelial cell (HPMEC)s, NEU1 sialidase associates with and desialylates the src family kinase (SFK) substrate, CD31, and disrupts angiogenesis. We asked whether the NEU1-CD31 interaction might be SFK-driven. We found that normalized phospho-SFK (PY416) signal is increased in postconfluent HPMECs compared to subconfluent cells and prior SFK inhibition with PP2 or SU6656 completely blocked NEU1 association with and desialylation of CD31. Prior silencing of each of the four SFKs expressed in HPMECs, as well as CD31, dramatically reduced confluence-induced SFK activation. No increases in tyrosine phosphorylation of NEU1 or CD31 were detected. However, in postconfluent cells, we found increased tyrosine phosphorylation of a 120 kDa protein that was identified as p120 catenin (p120ctn). Prior silencing of c-src, fyn, or yes each reduced p120ctn phosphorylation. Prior knockdown of p120ctn prevented NEU1-CD31 association in both co-immunoprecipitation and pull-down assays. In these same assays, p120ctn associated with each of the four HPMEC-expressed SFKs as well as CD31 and NEU1. The CD31-p120ctn interaction was SFK-dependent whereas the NEU1-p120ctn interaction was not. Using purified recombinant binding partners in a cell-free system, direct protein-protein interactions between NEU1, CD31, and p120ctn were detected. Our combined data indicate that as HPMECs achieve confluence and CD31 ectodomains become homophilically engaged, multiple SFKs are activated to increase tyrosine phosphorylation of p120ctn, which in turn, functions as a cross-bridging adaptor molecule that physically couples NEU1 to CD31, permitting NEU1-mediated desialylation of CD31. These findings establish a SFK-driven, p120ctn-dependent mechanism for NEU1 recruitment to CD31. [ABSTRACT FROM AUTHOR]

It is common to improve the adaptability and contact effect by using elastomer materials to fabricate triboelectric nanogenerator (TENG). However, compared with the traditional TENG made of non-elastomer, the existing design still has the disadvantages of low output and cumbersome preparation. This study proposes a TENG based on stacking teflon (PTFE), fluoro rubber (FKM), and polyurethane sponge (PU), the TENG not only combines the advantages of an elastomer and non-elastomer but also integrates the triboelectric characteristics of three materials to achieve higher performance. The stacked PTFE/FKM/PU TENG in single-electrode mode achieves a maximum instantaneous open-circuit voltage of 1250 V and short-circuit current of 108.9 μA, driving more than 1000 LEDs. It can also effectively recognize motion of flapping, wiping, squeezing, scratching, clicking, or poking by specific output feedback. Therefore, the stacked PTFE/FKM/PU TENG is expected to contribute to collection of discrete energy and development of human sensing technology. [Display omitted] • A stacking structure makes the TENG obtain better triboelectric characteristics. • The TENG inherits the elastic deformation capacity of PU. • In single electrode mode, the TENG also has strong output and applicability. • Just in contact with human skin, more than 1000 LEDs can be driven by the TENG. • The TENG can produce obvious output feedback for different hand movements. [ABSTRACT FROM AUTHOR]

Gram-negative bacteria release lipopolysaccharide (LPS) into the bloodstream. Here, it engages Toll-like receptor (TLR) 4 expressed in human lung microvascular endothelia (HMVEC-Ls) to open the paracellular pathway through Src family kinase (SFK) activation. The signaling molecules that couple TLR4 to the SFK-driven barrier disruption are unknown. In HMVEC-Ls, siRNA-induced silencing of TIRAP/Mal and overexpression of dominant-negative TIRAP/Mal each blocked LPS-induced SFK activation and increases in transendothelial [14C]albumin flux, implicating the MyD88-dependent pathway. LPS increased TRAF6 autoubiquitination and binding to IRAK1. Silencing of TRAF6, TRAF6-dominant-negative overexpression, or preincubation of HMVEC-Ls with a cell-permeable TRAF6 decoy peptide decreased both LPS-induced SFK activation and barrier disruption. LPS increased binding of both c-Src and Fyn to GST-TRAF6 but not to a GST-TRAF6 mutant in which the three prolines in the putative Src homology 3 domain-binding motif (amino acids 461-469) were substituted with alanines. A cell-permeable decoy peptide corresponding to the same proline-rich motif reduced SFK binding to WT GST-TRAF6 compared with the Pro → Ala-substituted peptide. Finally, LPS increased binding of activated Tyr(P)416-SFK to GST-TRAF6, and preincubation of HMVEC-Ls with SFK-selective tyrosine kinase inhibitors, PP2 and SU6656, diminished TRAF6 binding to c-Src and Fyn. During the TRAF6-SFK association, TRAF6 catalyzed Lys63-linked ubiquitination of c-Src and Fyn, whereas SFK activation increased tyrosine phosphorylation of TRAF6. The TRAF6 decoy peptide blocked both LPS-induced SFK ubiquitination and TRAF6 phosphorylation. Together, these data indicate that the proline-rich Src homology 3 domain-binding motif in TRAF6 interacts directly with activated SFKs to couple LPS engagement of TLR4 to SFK activation and loss of barrier integrity in HMVEC-Ls. [ABSTRACT FROM AUTHOR]

Thrombospondin (TSP) 1 is a trimeric multidomain protein that contains motifs that recognize distinct host cell receptors coupled to multiple signaling pathways. Selected TSP1-induced cellular responses are tyrosine kinasedependent, and TSP! contains epidermal growth factor (EGF)-like repeats. Specific receptor interactions or functions for the EGF-like repeats have not been identified. We asked whether one or more biological responses to TSP! might be explained through EGF receptor (EGFR) activation. In A431 cells, TSP! increased autophosphorylation of Tyr1068 of EGFR in a doseand time-dependent manner. The ability of TSP1 to activate EGFR was replicated by the tandem EGF-like repeats as a recombinant protein. The three EGFlike repeats alone produced a high level of Tyr-1068 phosphorylation. EGF-like repeats from TSP2 and TSP4 also activated EGFR. Tyr-1068 phosphorylation was less when individual EGF-like repeats were tested or flanking sequences were added to the three EGF-like repeats. TSP1 and its EGF-like repeats also increased phosphorylation of EGFR Tyr-845, Tyr-992, Tyr-1045, Tyr-1086, and Tyr-1173, activated phospholipase Cγ, and increased cell migration. No evidence was found for binding of the EGF-like repeats to EGFR. Instead, EGFR activation in response to TSP1 or its EGF-like repeats required matrix metalloprotease activity, including activity of matrix metalloprotease 9. Access to the ligand-binding portion of the EGFR ectodomain was also required. These findings suggest release of an endogenous EGFR ligand in response to ligation of a second unknown receptor by the TSPs. [ABSTRACT FROM AUTHOR]

• A label-free, enzyme-free biosensor was developed using aptamer-regulated DNA-silver nanoclusters (AgNCs). • AgNCs emitting strong red fluorescence was successfully synthesized via template screening. • The fluorescence of AgNCs can be regulated by OTA-aptamer and OTA due to conformational switch. • The point-of-care testing of OTA was realized in 45 min with a LOD of 1.3 nM. Mycotoxin contamination in cereal is a global concern, threatening food safety and human health, necessitating the development of rapid on-site methods. Here, a label- and enzyme-free biosensor was developed based on aptamer-regulated DNA-silver nanoclusters (AgNCs) for rapid detection of ochratoxin A (OTA). A novel DNA-templated AgNCs emitting strong red fluorescence was designed and synthesized in this study. The partial sequence of the DNA template was selected from the complementary OTA aptamer (Apt-OTA) sequence, which can quench fluorescence from the AgNCs via hybridization in the absence of OTA. In the presence of OTA, the high OTA-Aptamer affinity prevented the Apt-OTA from quenching the AgNCs, resulting in "turn on" of the fluorescence. This biosensor eliminated the use of costly reagents, complex pretreatments, and sophisticated equipment, which could realize the point-of-care testing (POCT) of OTA with a limit of detection (LOD) of 1.3 nM and a detection time of 45 min. [ABSTRACT FROM AUTHOR]

• Dual quantum dot nanobeads were applied in fluorescence-linked immunosorbent assay (QBs-FLISA). • Two target mycotoxins can be detected simultaneously by QBs-FLISA. • Compared with ELISA, detection sensitivity of QBs-FLISA was increased and detection duration was decreased. • QBs-FLISA was successfully applied in feedstuff analysis. A novel dual quantum dot nanobeads-based fluorescence-linked immunosorbent assay (QBs-FLISA) was successfully developed for simultaneously detecting aflatoxin B1 (AFB1) and zearalenone (ZEN) in feedstuffs. Dual CdSe/ZnS quantum dot nanobeads with different diameters that emit red and green fluorescence were conjugated with anti-AFB1 and anti-ZEN monoclonal antibodies to prepare fluorescent probes, which greatly enhance analytical performance. Under the optimal conditions, the limits of detection for AFB1 and ZEN were 9.3 and 102.1 pg mL−1, respectively. The recoveries ranged from 82.50% to 116.21% with relative standard deviation less than 11.3%. Compared with traditional enzyme-linked immunosorbent assay, detection sensitivities of AFB1 and ZEN using QBs-FLISA were increased 20 and 5 folds, respectively. In addition, results of feedstuff samples analyzed by QBs-FLISA and liquid chromatography tandem mass spectrometry showed a good agreement (R2 = 0.99). [ABSTRACT FROM AUTHOR]