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The ability of plants to tolerate stress is determined in part by the carbon allocated to their reserves. We studied two common urban tree species in northeastern North America, Acer saccharinum (Silver maple, native) and Acer platanoides (Norway maple, exotic), to assess the dynamics of non-structural carbohydrate (NSC) concentrations immediately following a maintenance pruning of 30% of the tree crown. NSC concentrations were measured by high-performance liquid chromatography in branches, main stems, and root tissues for both pruned and un-pruned trees at three intervals during the growing season. NSC concentrations in tree organs of A. platanoides were 75% higher than in A. saccharinum . Maintenance pruning did not have any significant depletion effect on carbohydrate concentrations in the tissues of either species. Yet, there was a significant increase in the NSC concentrations in un-pruned branches of pruned trees of A. platanoides at the end of the growing season, but no effect was observed in A. saccharinum . Higher levels of carbohydrates after pruning in woody plant tissues suggested that A. platanoides may have compensatory mechanisms that allow this species to respond better to maintenance pruning than A. saccharinum . [ABSTRACT FROM AUTHOR]

Highlights: [•] OPCs continue to give rise to myelinating oligodendrocytes in adulthood. [•] OPCs are activated by the inflammatory response to myelin debris. [•] OPCs follow the sequence: activation, proliferation, and differentiation. [•] Similar processes steer adult remyelination and white matter plasticity. [ABSTRACT FROM AUTHOR]

Candida albicans distinguishing features such as dimorphism and biofilm formation are thought to play a key role in oral tissue invasion and resistance to host defences and antifungal agents. In this study, we investigated the effect of 4-hydroxycordoin, a natural isopentenyloxychalcone, on growth, biofilm formation and yeast-hyphal transition of C. albicans. Serial dilutions of 4-hydroxycordoin in YNB medium were prepared in microplates to determine minimal inhibitory concentrations (MIC) and effects on biofilm formation for two strains of C. albicans. 4-Hydroxycordoin at up to 200[mu]g/ml had no effect on growth of C. albicans. Biofilm formation was strongly inhibited (>85%) by 4-hydroxycordoin at 20[mu]g/ml, while concentrations ranging from 50 to 200[mu]g/ml caused a significant inhibition of yeast-hyphal transition, as determined by microscopic observation. In conclusion, 4-hydroxycordoin exerts inhibitory effects on two important virulence factors of C. albicans: biofilm formation or yeast-hyphal transition. This suggests that 4-hydroxycordoin may have a therapeutic potential for C. albicans infections. [ABSTRACT FROM AUTHOR]

Abstract: Food restriction has been shown to be beneficial for a number of brain processes. In the current study, we characterized the impact of food restriction on hippocampal damage 70 days following ischemia. We assessed memory and cognitive flexibility of ad libitum fed (AL) and food-restricted (FR) animals using complex delayed non-matching- and matching-to-sample tasks in the radial arm maze. Our findings demonstrate that food restriction led to significant improvement of ischemia-induced memory impairments. FR ischemic animals rapidly reached comparable performance as both AL and FR sham animals in delayed-non-matching (win-shift) and matching (win-stay) radial arm maze tasks. They also made considerably fewer microchoices in the retention trials than AL ischemic animals. In contrast, AL ischemic rats showed persistent spatial memory impairments in the same paradigms. Assessment of basal and stress-induced corticosterone (CORT) secretion revealed no significant differences in baseline levels in AL and FR rats prior to or following global ischemia. However, FR animals showed a more pronounced attenuation of CORT secretion 45 min following restraint. Both FR and AL ischemic rats had comparable cell loss within CA1 and CA3 subfields of Ammon''s horn (CA1 and CA3) at 70 days following reperfusion, although a trend toward increased CA3 cell survival was observed in FR ischemic rats. The functional sparing in the FR ischemic animals in the face of equivalent hippocampal cell loss suggests that food restriction somehow enhanced the efficacy of remaining hippocampal or extrahippocampal neurons following ischemia. In the current study, this phenomenon was not associated with diet- and or ischemia-related alterations of vesicular glutamate transporter 1 expression in various hippocampal regions although lower vesicular GABA transporter immunostaining was present in the CA1 stratum oriens and the CA3 stratum radiatum in FR sham and ischemic rats. [Copyright &y& Elsevier]

Abstract: Ice storms are frequent natural disturbance events in hardwood forests of eastern Canada and the United States, but their effects on forest dynamics are not well understood. Our objectives were to characterize short- and long-term tree species dynamics after a severe ice storm, and to assess the influence of spatial distribution of trees on these dynamics. SORTIE, a spatially explicit individual tree-based forest model, was used to simulate the effects of a severe ice storm on 300 years old stands. Crown radius was reduced and tree mortality was increased for a 5-year period following the ice storm disturbance. To investigate the influence of the spatial distribution of trees, we repeated the same experiment in a uniformly distributed stand where we systematically assigned coordinates of all trees, saplings and seedlings before the ice storm was modeled. Our results showed that six types of dynamics can be adopted by a species following an ice storm and that spatial distribution of trees influenced the species responses. In summary, we found that a combination of factors, namely, species density and spatial distribution, shade tolerance, growth rate, extent of canopy openness and canopy loss resulting from the ice storm, determine how tree species respond to ice storm disturbance. [Copyright &y& Elsevier]

Investigates the effects of commercial thinning and fertilizer application six years after treatment on the structure, composition and nutrient concentrations of understorey vegetation in a Eucalyptus sieberi regrowth in East Gippsland. Effect of fertilizer application on species diversity and richness of the understorey; Implication of commercial thinning for growth of herbaceous species; Influence of phosphorus on the understorey.

• Trees vitality prior pruning influences epicormic branches development after pruning. • Discolored wood of the pruning cut is influenced by trees vitality prior pruning. • Less efficient compartmentalizing species have reduced stress-recovery resilience. • Tree vitality before pruning needs to be assessed to better predict the return pruning time. Reduction pruning of main tree stems is commonly performed in the maintenance of an electricity distribution network to encourage the growth of scaffold limbs away from the wires. Understanding both the initiation and growth of epicormic branch after reduction pruning interventions, as well as the area of discolored wood at the cutting point are important for optimizing the pruning cycle and maintaining safe trees. In this study, we investigated post-pruning traumatic responses in relation to tree vitality before pruning in 116 hackberries (Celtis occidentalis L.) and 86 green ash (Fraxinus pennsylvanica Marsh.) growing in a controlled environment of a nursery. Four years before pruning, each tree of both species was assigned a degree of damage commonly occurring in urban areas, including root injury, defoliation, and stem injury, for a total of 18 treatment combinations arranged in a random block design. In both species, trees that had lower vitality prior to pruning had a reduced number, height, and volume of the epicormic branches at the cutting point two years after main stem pruning. Interestingly and contrary to expectations, a positive relationship was found between the area of discolored wood at the cutting point and tree vitality prior to pruning. However, below a vitality threshold, the area of wood discoloration was exacerbated by crown dieback and retrenchment. Celtis showed a less efficient ability to compartmentalize and produced fewer epicormic branches compared to Fraxinus , and epicormic branches were of reduced height and volume when low growth was observed. From a management perspective, although both species can survive after several damage events and after reduction of the main stem, the results suggest to conduct an assessment of tree vitality before pruning to identify trees with lower vitality and better predict the return pruning time. [ABSTRACT FROM AUTHOR]

A family of seven facilitative glucose transporters (Glut1–5, 7 and 8) mediates the cellular uptake of glucose. In the brain, Glut2, Glut5 and Glut8 are found at relatively low levels whereas Glut1, Glut3 and Glut4 were reported in abundance in several brain regions. Using immunofluorescence, this study investigated, compared and quantified the localization of the brain major glucose transporters, Glut1, Glut3 and Glut4, in the different cerebral areas of CD1 mice. Most of the staining of Glut1, Glut3 and Glut4 in the mouse brain coincides with observations made in rats. The results confirm the cortical neuropil distribution of Glut3, the prominence of this transporter in the mossy fiber field of the hippocampus and the Glut3 and Glut4 immunostaining of the hippocampal pyramidal cell layer. The present study also reports novel localizations of the transporters such as the presence of Glut3 in neuronal perikarya, Glut4-labeled neurons in the CA3 of the hippocampus and the subiculum. In the cerebellum, Glut3 shows subcellular localization to the base of the Purkinje cell bodies near the axon hillock. Furthermore, an important population of Golgi cells was found to be strongly immunostained for Glut4 in the granular cell layer of the cerebellum. The quantification results suggest that the relative abundance of Glut1 in the frontal and motor cortices coincides well with the high-energy demands of these brain regions. However, the Glut4-selective abundance in cerebral motor areas supports its suggested role in providing the energy needed for the control of the motor activity. The reported neuropil distribution of Glut3 seems to uphold its suggested role in synaptic energy provision and neurotransmitter synthesis.We conclude that the cellular and regional distributions of the glucose transporters in the rodent brain seem to be relevant to their corresponding functions. [Copyright &y& Elsevier]

Abstract: K-ATP channels formed of the Sur and Kir subunits are widely distributed in the brain. Sur1–Kir6.2 is the most common combination of K-ATP channel subunits in the brain and Kir6.2 plays an important role in glucose metabolism through pancreatic insulin secretion or hypothalamic glucose sensing. K-ATP channels have also been reported to play a role in memory processing. Therefore, the aim of the present experiment is to assess the gene and protein expression of GLUT1, GLUT3 and GLUT4 in various brain regions of Kir6.2−/− K-ATP knockout mice and to test their working memory performance. GLUT4 was measured using two antibodies, one recognizing an intracellular epitope and the other, an extracellular epitope. Relative to their corresponding wild type, semi-quantitative immunohistochemistry showed that GLUT4 protein expression as measured by a GLUT4 antibody recognizing an extracellular epitope was increased in the Kir6.2−/− K-ATP mice. However, there was only a small increase in GLUT4 labeling using the GLUT4 antibody recognizing the intracellular epitope. These results suggest a compensatory higher GLUT4 inclusion at the cellular neuronal membrane in the cerebral cortex, hippocampus and cerebellum of the Kir6.2−/− K-ATP knockout mice. However, there was no change in GLUT4 gene expression assessed by TaqMan PCR except for a decrease in the cerebellum of these mice. Working memory performance of the Kir6.2−/− K-ATP mice was disrupted at age of 12 weeks but not at 5 weeks. The mild glucose intolerance that is observed in the Kir6.2 knockout mice is unlikely to have created the memory deficits observed. Rather, in light of the effects of K-ATP channel modulators on memory, the memory deficits in the Kir6.2−/− K-ATP mice are more likely due to the absence of the Kir6.2 and possible disruption of the GLUT4 activity in the brain. [Copyright &y& Elsevier]

Abstract: Various types of learning, including operant conditioning, induce an increase in cellular activation concomitant with an increase in local cerebral glucose utilization (LCGU). This increase is mediated by increased cerebral blood flow or changes in brain capillary density and diameter. Because glucose transporters are ultimately responsible for glucose uptake, we examined their plastic expression in response to cellular activation. In vitro and in vivo studies have demonstrated that cerebral glucose transporter 1 (GLUT1) expression consistently parallels changes in LCGU. The present study is the first to investigate the effect of memory processing on glucose transporters expression. Changes in GLUT expression produced by training in an operant conditioning task were measured in the brain of CD1 mice. Using semi-quantitative immunohistochemistry, Western blot and real time RT-PCR the cerebral GLUT1 and GLUT3 expression was quantified immediately, 220 min and 24 h following training. Relative to sham-trained and naive controls, operant conditioning training induced an immediate increase in GLUT1 immunoreactivity level in the hippocampus CA1 pyramidal cells as well as in the sensorimotor cortex. At longer post-learning delays, GLUT1 immunoreactivity decreased in the sensorimotor cortex and putamen. Parallel to the changes in protein levels, hippocampus GLUT1 mRNA level also increased immediately following learning. No effect of learning was found on hippocampal GLUT3 protein or mRNA expression. Measures of changes in glucose transporters expression present a link between cellular activation and glucose metabolism. The learning-induced localized increases in GLUT1 protein as well as mRNA levels observed in the present study confirm the previous findings that GLUT1 expression is plastic and respond to changes in cellular metabolic demands. [Copyright &y& Elsevier]