Search

Your search keyword '"Maugé D"' showing total 1 results
Start Over Author "Maugé D" Publisher elsevier b.v.
1 results on '"Maugé D"'

1. Electrochemical DNA-biosensors based on long-range electron transfer: optimization of the amperometric detection in the femtomolar range using two-electrode setup and ultramicroelectrode.

Author

Nassi, A., Guillon, F.-X., Amar, A., Hainque, B., Amriche, S., Maugé, D., Markova, E., Tsé, C., Bigey, P., Lazerges, M., and Bedioui, F.

Subjects
*AMPEROMETRIC sensors, *CHARGE exchange, *BIOSENSORS, *ULTRAMICROELECTRODES, *STRUCTURAL optimization, *ELECTROCHEMICAL analysis
Abstract

We report on the design of electrochemical DNA-biosensors made from 15, 25 and 50 μm diameter gold ultramicroelectrodes (UMEs) in a two-electrode setup. The biosensors were prepared by chemical adsorption of 22-base thiol-labeled DNA-probes in a self-assembled monolayer configuration onto the surface of the gold UME. Hybridization events were transduced by Fe III /Fe II redox process electro-mediated by DNA-redox intercalated mediators. The optimization of the DNA-biosensors was achieved by using different Fe III /Fe II ligands (cyanide, cefadroxil, deferoxamine) and intercalators (methylene blue, proflavine). Optimized biosensors allowed direct and selective amperometric detection in the femtomolar range and of single mismatch within a 35 minutes protocol. [ABSTRACT FROM AUTHOR]

Published
2016
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results