4 results on '"McCallion, Andrew S."'
Search Results
2. Screening non-MAPT genes of the Chr17q21 H1 haplotype in Parkinson's disease.
- Author
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Soto-Beasley, Alexandra I., Walton, Ronald L., Valentino, Rebecca R., Hook, Paul W., Labbé, Catherine, Heckman, Michael G., Johnson, Patrick W., Goff, Loyal A., Uitti, Ryan J., McLean, Pamela J., Springer, Wolfdieter, McCallion, Andrew S., Wszolek, Zbigniew K., and Ross, Owen A.
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PARKINSON'S disease , *DNA copy number variations , *LINKAGE disequilibrium , *MICROTUBULE-associated proteins , *GENES , *BIOLOGICAL variation - Abstract
The microtubule-associated protein tau (MAPT) gene is considered a strong genetic risk factor for Parkinson's disease (PD) in Caucasians. MAPT is located within an inversion region of high linkage disequilibrium designated as H1 and H2 haplotype, and contains eight other genes which have been implicated in neurodegeneration. The aim of the current study was to identify common coding variants in strong linkage disequilibrium (LD) within the associated loci on chr17q21 harboring MAPT. Sanger sequencing of coding exons in 90 Caucasian late-onset PD (LOPD) patients was performed. Specific gene sequencing for LRRC37A , LRRC37A2 , ARL17A and ARL17B was not possible given the high homology, presence of pseudogenes and copy number variants that are in the region, and therefore four genes (NSF , KANSL1 , SPPL2C , and CRHR1) were included in the analysis. Coding variants from these four genes that did not perfectly tag (r2 = 1) the MAPT H1/H2 haplotype were genotyped in an independent replication series of Caucasian PD cases (N = 851) and controls (N = 730). In the 90 LOPD cases we identified 30 coding variants. Eleven non-synonymous variants tagged the MAPT H1/H2 haplotype, including two SPPL2C variants (rs12185233 and rs12373123) that had high pathogenic combined annotation dependent depletion (CADD) scores of >20. In the replication series, the non-synonymous KANSL1 rs17585974 variant was in very strong LD with MAPT H1/H2 and had a high CADD score of 24.7. We have identified several non-synonymous variants across neighboring genes of MAPT that may warrant further genetic and functional investigation within the biological etiology of PD. • MAPT H1 is a consistent Parkinson's disease association locus on chr17q21. • Chr17q21H1 extended haplotype is a complex genetic region of inversion. • Other genes/variants in complete linkage disequilibrium may be responsible. • Potentially damaging variants in KANSL1 , NSF and SPPL2C. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
3. Carbamate nerve agent prophylatics exhibit distinct toxicological effects in the zebrafish embryo model.
- Author
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Fischer, Audrey, Wolman, Marc, Granato, Michael, Parsons, Michael, McCallion, Andrew S., Proescher, Jody, and English, Emily
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CARBAMATES , *PYRIDOSTIGMINE bromide , *MYASTHENIA gravis treatment , *ACETYLCHOLINESTERASE inhibitors , *LABORATORY zebrafish - Abstract
Pyridostigmine bromide (PB) is an FDA-approved drug for the treatment of myasthenia gravis and a prophylactic pre-treatment for organophosphate nerve agent poisoning. Current methods for evaluating nerve agent treatments include enzymatic studies and mammalian models. Rapid whole animal screening tools for assessing the effects of nerve agent pre-treatment and post-exposure drugs represent an underdeveloped area of research. We used zebrafish as a model for acute and chronic developmental exposure to PB and two related carbamate acetylcholinesterase (AChE) inhibitors, neostigmine bromide (NB) and physostigmine (PS). Lethal doses and gross morphological phenotypes resulting from exposure to sub-lethal doses of these compounds were determined. Quantitative analyses of motility impairment and AChE enzyme inhibition were used to determine optimal dosing conditions for evaluation of the effects of carbamate exposures on neuronal development; ~ 50% impairment of response to startle stimuli and > 50% inhibition of AChE activity were observed at 80 mM PB, 20 mM NB and 0.1 mM PS. PB induced stunted somite length, but no other phenotypic effects were observed. In contrast, NB and PS induced more severe phenotypic morphological defects than PB as well as neurite outgrowth mislocalization. Additionally, NB induced mislocalization of nicotinic acetylcholine receptors, resulting in impaired synapse formation. Taken together, these data suggest that altered patterns of neuronal connectivity contribute to the developmental neurotoxicity of carbamates and demonstrate the utility of the zebrafish model for distinguishing subtle structure-based differential effects of AChE inhibitors, which include nerve agents, pesticides and drugs. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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4. A zebrafish SKIV2L2-enhancer trap line provides a useful tool for the study of peripheral sensory circuit development
- Author
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Cox, Jane A., McAdow, Anthony R., Dinitz, Amy E., McCallion, Andrew S., Johnson, Stephen L., and Voigt, Mark M.
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ZEBRA danio , *LATERAL line organs , *CELLULAR mechanics , *PERIPHERAL nervous system , *GREEN fluorescent protein , *GENE expression , *NEURAL circuitry - Abstract
Abstract: The zebrafish is an ideal model for elucidating the cellular and molecular mechanisms that underlie development of the peripheral nervous system. A transgenic line that selectively labels all the sensory circuits would be a valuable tool for such investigations. In this study, we describe such a line: the enhancer trap zebrafish line Tg(SKIV2L2:gfp) j1775 which expresses green fluorescent protein (gfp) in the peripheral sensory ganglia. We show that this transgene marks all peripheral ganglia and sensory nerves, beginning at the time when the neurons are first extending their processes, but does not label the efferent nerves. The trapped reporter is inserted just upstream of a previously poorly described gene: lhfpl4 on LG6. The expression pattern of this gene by in situ hybridization reveals a different, but overlapping, pattern of expression compared to that of the transgene. This pattern also does not mimic that of the gene (skiv2l2), which provided the promoter element in the construct. These findings indicate that reporter expression is not dictated by an endogenous enhancer element, but instead arises through an unknown mechanism. Regardless, this reporter line should prove to be a valuable tool in the investigation of peripheral nervous system formation in the zebrafish. [Copyright &y& Elsevier]
- Published
- 2011
- Full Text
- View/download PDF
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