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We tested whether glutamate and/or testosterone administration to pubertal Dorper rams would increase their apptetitive sexual behavior (ASB) and consummatory sexual behavior (CSB). We then evaluated their ability to induce the sexual response of nulliparous-anestrous ewes during early spring (26° N). Dorper rams (n = 20, 11 months old) were distributed in four groups homogeneous regarding live weight ([LW], 55 ± 0.09 kg), body condition score (3.2 ± 0.1 units), and scrotal circumference (30 ± 0.3 cm) and received the following treatments of: 1) intramuscular glutamate (GG); 7 mg kg−1 LW of glutamate (every 3 days × 28 days; iv), 2) testosterone treated (GT); 1 ml of saline (every 3 days × 15 days; iv) + 25 mg of testosterone (every 3 days × 15 days; im), 3) glutamate + testosterone (GGT); 7 mg kg−1 LW of glutamate (every 3 days × 28 days; iv) + 25 mg testosterone (every 3 days × 15 days; im), and 4) GC; 1 mL of saline (every 3 days × 28 days; iv). Thereafter, nulliparous-anestrus Dorper ewes (n = 80; LW 42.2 ± 2.3 kg, body condition score 3 ± 0.09 units) were exposed to these treated males. On male-to-female contact, a sexual behavioral test (2 hours × 2 days) was conducted to evaluate the ASB and CSB frequencies. Afterward, the female reproductive performance (interval to estrus, estrus response, ovulation response, ovulation rate, and pregnancy rate) was quantified. Although the GGT-males had the best ASB and ASB+CSB (P < 0.05), the GT-males had the best CSB (P < 0.05). The estrus response, ovulation response, and ovulation rate did not differ (P < 0.05) among groups, yet, interval to estrus and pregnancy rate favored (P < 0.05) to the ewes-GGT rams. Our results revealed that the ASB and CSB of young Dorper rams were improved by frequent GGT administration. Moreover, the shortest onset to estrus and the largest pregnancy rate were observed in those ewes exposed to the GGT-treated pubertal rams. Such results are encouraging from a comparative sexual behavior standpoint, while may hold potential translational applications. [ABSTRACT FROM AUTHOR]

The aim of this study was to determine if exogenous administration of glutamate and (or) testosterone to male rams during the season of reproductive arrest is able to re-activate male sexual behavior and, later on, to promote through the male effect, both sexual and reproductive competence of anovulatory nulliparous ewes. Therefore, an experiment was performed under long-day photoperiods (spring; photo-reproductive arrest, 26°N). Dorper rams were randomly divided into four homogeneous experimental groups (n = 5 males each) regarding live weight (LW), body condition score (BCS), scrotal circumference (SC) and odor intensity (OI). Then, groups were treated with: i) GG (7 mg kg −1 LW of glutamate, every 4d × 30d, im.), ii) GGT (7 mg kg −1 LW of glutamate every 4d × 30d im + 25 mg of testosterone propionate, every 3d × 15d, im.), iii) GT (25 mg of testosterone propionate every 3d × 15d, im, and iv) GC (1 mL of saline, every 4d × 30d, im.). Thereafter, Dorper rams, (n = 4 per group) were selected and exposed to Dorper anovulatory-nulliparous ewes divided in four groups (n = 14 ewes each), and all the appetitive (ASB) and consummatory (CSB) sexual behaviors and indicators of sexual rest (ISR) were registered during the first 48 h of this male-to-females contact. Thereafter, males continued the male-to-female contact for another 8 d, in order to quantify the ewe's sexual and reproductive response through the male effect. During the sexual behavior tests, the GGT rams showed the highest ASB + CSB frequencies (P < 0.05) followed by the GG-rams with the lowest frequencies showed by the GC and GT groups. While the highest ISR behaviors were shown by the GG and GGT groups (P < 0.05) followed by the GC and GT-rams, no differences occurred regarding LW, BCS, and SC along the experimental breeding, with the largest (P < 0.05) OI shown by the GGT-rams and the lowest value observed in the control rams. Regarding the reproductive response of the nulliparous-anovulatory ewes exposed to treated rams, the glutamate + testosterone treated males induced not only an increased (P < 0.05) ovulatory activity and faster (P < 0.05) estrus response but an augmented (P < 0.05) pregnancy rate of the previously anovulatory ewes. To conclude, whereas the GG and GGT treatments generated encouraging sexual and reproductive outcomes, our results are also thought-provoking from a comparative sexual behavior perspective while may embrace potential applications to other animal industries. [ABSTRACT FROM AUTHOR]

Abstract The aim of this study was to evaluate the effect of the stage of seasonal anestrus and breed on ovarian response in non-estrous cycling goats using a progesterone (P4) injection plus human chorionic gonadotropin (hCG)-based protocol. In Experiment 1, non-estrous cycling local Mexican goats were treated with 20 mg of P4 plus 100 IU of hCG injections 24 h apart during April (early anestrus, n = 13) or June (late anestrus, n = 12). The estrous response, interval from hCG-to-estrus, and interval to ovulation were not affected by season (P > 0.05). In addition, the size of the follicle from which ovulation occurred and the size of the corpus luteum were not different between the two stages of seasonal anestrus (P > 0.05). In Experiment 2, the estrous response was compared between multiparous non-estrous cycling local Mexican (n = 18) and Alpine (n = 19) goats in which stage of the estrous cycle was synchronized using the same P4+hCG protocol as in Experiment 1. Neither the onset of estrus nor the time of ovulation differed between breeds, and the estrus-to-ovulation interval also was similar for both breeds (P > 0.05). The diameter of the dominant follicle at the time of ovulation was similar between local and Alpine goats (P > 0.05). In addition, the pregnancy rate was not different for both local and Alpine goats (P > 0.05). In conclusion, results of this study indicate that the stage of seasonal anestrus or breed do not modify estrous and ovarian response in non-estrous cyclic goats synchronized with a P4 injection plus hCG-based protocol. [ABSTRACT FROM AUTHOR]

Abstract The objective of this study was to determine the estrous response and the moment of ovulation and fertility after a progesterone (P4) priming plus human chorionic gonadotropin (hCG) administration in multiparous and nulliparous goats. Therefore, two experiments were conducted during non-breeding season (April and May, 26° N) and all the animals received a single injection of 20 mg of P4 and 100 IU of hCG 24 h later. In Experiment 1, 13 multiparous and 9 nulliparous goats were subjected to estrus detection twice a day from P4 administration, and their ovaries were scanned by transrectal ultrasonography every 12 h from the onset of estrus to determine ovulation. The proportion of goats in estrus did not differ between multiparous and nulliparous females. The onset of estrus (60.5 ± 12.4 h vs. 52.0 ± 5.2 h after hCG administration) and the moment of ovulation (91.5 ± 10.3 h vs. 85.3 ± 11.5 h) were also similar in multiparous and nulliparous goats. In Experiment 2, a total of 299 multiparous and nulliparous goats managed under intensive (n = 112 and 41 goats, respectively) or extensive (n = 85 and 61 goats, respectively) production systems were divided to receive a fixed-time artificial insemination (FTAI) with fresh semen at 60 or 72 h after hCG administration. The pregnancy rate did not differ significantly between multiparous and nulliparous goats in both production systems. Nonetheless, in the intensive system pregnancy rate was affected by the moment of insemination (P < 0.05). In this system, the pregnancy rate was higher in goats inseminated at 60 h than in 72 h (55.6%, 44/79 vs. 35.1%, 26/74, respectively; P < 0.05). On the contrary, in the extensive system the pregnancy rate was not affected by the time of insemination (29.4%, 23/78 vs. 22.0%, 15/68). To conclude, both the ovulatory response and the pregnancy rate after a single P4 injection plus hCG was similar between multiparous and nulliparous goats during anovulatory season. Although the pregnancy rate was not affected by the time of insemination in the extensive production system, under intensive conditions FTAI should be performed at 60 h after hCG treatment. Highlights • A single P4 injection plus hCG-based protocol for FTAI in goats was evaluated. • The protocol was effective to control estrus and ovulation in both multiparous and nulliparous goats. • Pregnancy rate after FTAI was not affected by parity. • In the intensive production system, pregnancy rate was greater in goats inseminated at 60 h after hCG administration. • Greater fertility was obtained in intensive than extensive production system. [ABSTRACT FROM AUTHOR]

Nutritional requirements of sheep during late gestation increase as a consequence of high fetal growth, mammary tissue development and colostrum synthesis. While prepartum energy supplementation is a nutritional strategy to improve lamb postnatal performance in thermoneutral environments, this has not been studied under heat stress. This study aimed to evaluate effects of maternal energy supplementation during the last third of pregnancy on post-weaning feedlot performance and thermoregulation capacity of heat-stressed male lambs born from multiple births. Twenty Dorper x Pelibuey entire male lambs with initial body weight of 18.2 ± 0.4 kg, aged 2.7 mo (weaned) and born in multiple pregnancies were used in a 32 d feeding study. Treatments were based in the prenatal origin of the lambs: 1) ewes fed 100 ( n  = 10, Control) and 2) 125% ( n  = 10, Supplemented) of metabolizable energy requirements from day 100 of gestation to lambing. Lambs were housed outdoor in individual pens under summer environment conditions in an arid region (temperature = 36 ± 4.4 °C and temperature-humidity index = 81 ± 3.9 units). Overall feedlot performance was not affected by pre-partum maternal feeding, although lower (P = 0.04) growth rate and feed efficiency occurred during the last 8 d of the study in lambs born from supplemented ewes. Rectal temperature, respiratory rate and hair coat temperature during daytime were unaffected by prepartum supplementation. Serum concentrations of glucose, cholesterol, urea, total protein and thyroid hormones were similar between lambs born from control and supplemented ewes. It is concluded that, in hair sheep breeds, prepartum energy supplementation did not alter overall post-weaning productive performance and thermoregulation capacity of heat-stressed male lambs that were born in multiple pregnancies. [ABSTRACT FROM AUTHOR]

Rectal temperature (RT) is the foremost physiological variable indicating if an animal is suffering hyperthermia. However, this variable is traditionally measured by invasive methods, which may compromise animal welfare. Models to predict RT have been developed for growing pigs and lactating dairy cows, but not for pregnant heat-stressed ewes. Our aim was to develop a prediction equation for RT using non-invasive physiological variables in pregnant ewes under heat stress. A total of 192 records of respiratory frequency (RF) and hair coat temperature in various body regions (i.e., head, rump, flank, shoulder, and belly) obtained from 24 Katahdin×Pelibuey pregnant multiparous ewes were collected during the last third of gestation (i.e., d 100 to lambing) with a 15 d sampling interval. Hair coat temperatures were taken using infrared thermal imaging technology. Initially, a Pearson correlation analysis examined the relationship among variables, and then multiple linear regression analysis was used to develop the prediction equations. All predictor variables were positively correlated ( P <0.01; r =0.59–0.67) with RT. The adjusted equation which best predicted RT ( P <0.01; R adj 2 =56.15%; CV=0.65%) included as predictors RF and head and belly temperatures. Comparison of predicted and observed values for RT indicates a suitable agreement ( P <0.01) between them with moderate accuracy ( R adj 2 =56.15%) when RT was calculated with the adjusted equation. In general, the final equation does not violate any assumption of multiple regression analysis. The RT in heat-stressed pregnant ewes can be predicted with an adequate accuracy using non-invasive physiologic variables, and the final equation was: RT=35.57+0.004 (RF)+0.067 (heat temperature)+0.028 (belly temperature). [ABSTRACT FROM AUTHOR]