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Your search keyword '"Morais, Daniel Kumazawa"' showing total 2 results
Start Over Author "Morais, Daniel Kumazawa" Publisher elsevier b.v.
2 results on '"Morais, Daniel Kumazawa"'

2. Misguided phylogenetic comparisons using DGGE excised bands may contaminate public sequence databases.

Author

Pylro, Victor Satler, Morais, Daniel Kumazawa, Kalks, Karlos Henrique Martins, Roesch, Luiz Fernando Wurdig, Hirsch, Penny R., Tótola, Marcos Rogério, and Yotoko, Karla

Subjects
*PHYLOGENY, *MICROBIAL ecology, *NUCLEOTIDE sequence, *GENE amplification, *GEL electrophoresis, *COMPARATIVE studies
Abstract

Controversy surrounding bacterial phylogenies has become one of the most important challenges for microbial ecology. Comparative analyses with nucleotide databases and phylogenetic reconstruction of the amplified 16S rRNA genes from DGGE (Denaturing Gradient Gel Electrophoresis) excised bands have been used by several researchers for the identification of organisms in complex samples. Here, we individually analyzed DGGE-excised 16S rRNA gene bands from 10 certified bacterial strains of different species, and demonstrated that this kind of approach can deliver erroneous outcomes to researchers, besides causing/emphasizing errors in public databases. [ABSTRACT FROM AUTHOR]

Published
2016
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