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Human telomerase reverse transcriptase (hTERT) is an attractive target for anti-cancer therapies. We developed an effective method for generating hTERT-specific CD8+ T cells (hTERT-induced natural T cells [TERTiNTs]) using peripheral blood mononuclear cells (PBMCs) from patients with solid cancers and investigated their feasibility and safety. This was a single-center phase 1 trial using a 3 + 3 dose escalation design to evaluate six dose levels of TERTiNTs. PBMCs from each patient were screened using an hTERT peptide panel to select those that stimulated CD8+ T cells. The four most stimulatory peptides were used to produce autologous CD8+ T cells from patients refractory or intolerant to standard therapies. Eligible patients received a single intravenous infusion of TERTiNTs at different dose levels (4 × 108 cells/m2, 8 × 108 cells/m2 and 16 × 108 cells/m2). Pre-conditioning chemotherapy, including cyclophosphamide alone or in combination with fludarabine, was administered to induce lymphodepletion. From January 2014 to October 2019, a total of 24 patients with a median of three prior lines of therapy were enrolled. The most common adverse events were lymphopenia (79.2%), nausea (58.3%) and neutropenia (54.2%), mostly caused by pre-conditioning chemotherapy. The TERTiNT infusion was well tolerated, and dose-limiting toxicities were not observed. None of the patients showed objective responses. Seven patients (30.4%) achieved stable disease with a median progression-free survival of 3.9 months (range, 3.2–11.3). At the highest dose level (16 × 108 cells/m2), four of five patients showed disease stabilization. The generation of TERTiNTs was feasible and safe and provided an interesting disease control rate in heavily pre-treated cancer patients. [ABSTRACT FROM AUTHOR]

The article discusses a case of a 43-year-old man who was presented to a hospital with generalized skin pruriginous and papulonodular eruptions. It say that a skin biopsy was performed and found symptoms of hyperkeratosis, pseudo-epitheliomatous acanthosis, and vascular hyperplasia of the upper dermis that are characteristics of prurigo nodularis. It states that tapere 16 milligram (mg) dose of oral methylprednisolone, antibiotics, antihistamines, ad cyrotherapy was treated to the patient.