6 results on '"Petit, Aurélie"'
Search Results
2. Club cell secretory protein serum concentration is a surrogate marker of small-airway involvement in asthmatic patients.
- Author
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Bommart, Sebastien, Marin, Grégory, Molinari, Nicolas, Knabe, Lucie, Petit, Aurélie, Chanez, Pascal, Gamez, Anne Sophie, Devautour, Catherine, Vachier, Isabelle, and Bourdin, Arnaud
- Published
- 2017
- Full Text
- View/download PDF
3. The HDAC inhibitor SAHA does not rescue CFTR membrane expression in Cystic Fibrosis.
- Author
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Bergougnoux, Anne, Petit, Aurélie, Knabe, Lucie, Bribes, Estelle, Chiron, Raphaël, De Sario, Albertina, Claustres, Mireille, Molinari, Nicolas, Vachier, Isabelle, Taulan-Cadars, Magali, and Bourdin, Arnaud
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CYSTIC fibrosis treatment , *CYSTIC fibrosis transmembrane conductance regulator genetics , *HYDROXAMIC acids , *HISTONE deacetylase , *METAPLASIA - Abstract
The development of suitable Cystic Fibrosis (CF) models for preclinical bench tests of therapeutic candidates is challenging. Indeed, the validation of molecules to rescue the p.Phe508del-CFTR channel (encoded by the Cystic Fibrosis Transmembrane conductance Regulator gene carrying the p.Phe508del mutation) requires taking into account their overall effects on the epithelium. Suberoylanilide Hydroxamic Acid (SAHA), a histone deacetylase inhibitor (HDACi), was previously shown to be a CFTR corrector via proteostasis modulation in CFTR-deficient immortalized cells. Here, we tested SAHA effects on goblet cell metaplasia using an ex vivo model based on the air-liquid interface (ALI) culture of differentiated airway epithelial cells obtained by nasal scraping from CF patients and healthy controls. Ex vivo epithelium grew successfully in ALI cultures with significant rise in the expression of CFTR and of markers of airway epithelial differentiation compared to monolayer cell culture. SAHA decreased CFTR transcript and protein levels in CF and non-CF epithelia. Whereas SAHA induced lysine hyperacetylation, it did not change histone modifications at the CFTR promoter. SAHA reduced MUC5AC and MUC5 B expression and inhibited goblet epithelial cell differentiation. Similar effects were obtained in CF and non-CF epithelia. All the effects were fully reversible within five days from SAHA withdrawal. We conclude that, ex vivo, SAHA modulate the structure of airway epithelia without specific effect on CFTR gene and protein suggesting that HDACi cannot be useful for CF treatment. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
4. Lung development, regeneration and plasticity: From disease physiopathology to drug design using induced pluripotent stem cells.
- Author
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Ahmed, Engi, Sansac, Caroline, Assou, Said, Gras, Delphine, Petit, Aurélie, Vachier, Isabelle, Chanez, Pascal, De Vos, John, and Bourdin, Arnaud
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LUNG development , *PLURIPOTENT stem cells , *DRUG design , *EPITHELIAL cells , *FETAL development - Abstract
Lungs have a complex structure composed of different cell types that form approximately 17 million airway branches of gas-delivering bronchioles connected to 500 million gas-exchanging alveoli. Airways and alveoli are lined by epithelial cells that display a low rate of turnover at steady-state, but can regenerate the epithelium in response to injuries. Here, we review the key points of lung development, homeostasis and epithelial cell plasticity in response to injury and disease, because this knowledge is required to develop new lung disease treatments. Of note, canonical signaling pathways that are essential for proper lung development during embryogenesis are also involved in the pathophysiology of most chronic airway diseases. Moreover, the perfect control of these interconnected pathways is needed for the successful differentiation of induced pluripotent stem cells (iPSC) into lung cells. Indeed, differentiation of iPSC into airway epithelium and alveoli is based on the use of biomimetics of normal embryonic and fetal lung development. In vitro iPSC-based models of lung diseases can help us to better understand the impaired lung repair capacity and to identify new therapeutic targets and new approaches, such as lung cell therapy. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
5. Bronchial epithelium as a target for innovative treatments in asthma.
- Author
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Gras, Delphine, Chanez, Pascal, Vachier, Isabelle, Petit, Aurélie, and Bourdin, Arnaud
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ASTHMA treatment , *EPITHELIUM , *MEDICAL innovations , *HOMEOSTASIS , *IMMUNE response , *ACETYLCHOLINE , *BRONCHOALVEOLAR lavage - Abstract
Abstract: Increasing evidence of a critical role played by the bronchial epithelium in airway homeostasis is opening new therapeutic avenues. Its unique situation at the interface with the environment suggests that the subtle regulation orchestrated by the epithelium between tolerance and specific immune response might be impaired in asthma. Airway mucus is acting as a physical and a biological fluid between the environment and the epithelium, synergistically moved by the cilia. In asthma, excessive mucus production is a hallmark of airway remodeling. Since many years we tried to therapeutically target mucus hypersecretion, but actually this option is still not achieved. The present review discusses the dynamic processes regulating airway mucus production. Airway inflammation is central in current asthma management. Understanding of how the airway epithelium influences the TH2 paradigm in response to deleterious agents is improving. The multiple receptors expressed by the airway epithelium are the transducers of the biological signals induced by various invasive agents to develop the most adapted response. Airway remodeling is observed in severe chronic airway diseases and may result from ongoing disturbance of signal transduction and epithelial renewal. Chronic airway diseases such as asthma will require assessment of these epithelial abnormalities to identify phenotypic characteristics associated with predicting a clinical benefit for epithelial-directed therapies. [Copyright &y& Elsevier]
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- 2013
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6. Combining gene expression and genetic analyses to identify candidate genes involved in cold responses in pea.
- Author
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Legrand, Sylvain, Marque, Gilles, Blassiau, Christelle, Bluteau, Aurélie, Canoy, Anne-Sophie, Fontaine, Véronique, Jaminon, Odile, Bahrman, Nasser, Mautord, Julie, Morin, Julie, Petit, Aurélie, Baranger, Alain, Rivière, Nathalie, Wilmer, Jeroen, Delbreil, Bruno, and Lejeune-Hénaut, Isabelle
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GENE expression in plants , *PLANT genes , *PEAS , *EFFECT of temperature on plants , *PLANT growth , *POLYMERASE chain reaction - Abstract
Abstract: Cold stress affects plant growth and development. In order to better understand the responses to cold (chilling or freezing tolerance), we used two contrasted pea lines. Following a chilling period, the Champagne line becomes tolerant to frost whereas the Terese line remains sensitive. Four suppression subtractive hybridisation libraries were obtained using mRNAs isolated from pea genotypes Champagne and Terese. Using quantitative polymerase chain reaction (qPCR) performed on 159 genes, 43 and 54 genes were identified as differentially expressed at the initial time point and during the time course study, respectively. Molecular markers were developed from the differentially expressed genes and were genotyped on a population of 164 RILs derived from a cross between Champagne and Terese. We identified 5 candidate genes colocalizing with 3 different frost damage quantitative trait loci (QTL) intervals and a protein quantity locus (PQL) rich region previously reported. This investigation revealed the role of constitutive differences between both genotypes in the cold responses, in particular with genes related to glycine degradation pathway that could confer to Champagne a better frost tolerance. We showed that freezing tolerance involves a decrease of expression of genes related to photosynthesis and the expression of a gene involved in the production of cysteine and methionine that could act as cryoprotectant molecules. Although it remains to be confirmed, this study could also reveal the involvement of the jasmonate pathway in the cold responses, since we observed that two genes related to this pathway were mapped in a frost damage QTL interval and in a PQL rich region interval, respectively. [Copyright &y& Elsevier]
- Published
- 2013
- Full Text
- View/download PDF
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