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Abstract: San Daniele dry-cured hams (also known as prosciutto) are produced in the Northeastern region of Italy. This high value product requires high quality fresh meat to avoid processing problems. The Sterol Regulatory Element Binding Protein-1 (SREBF1) is a transcription factor involved in the regulation of fatty acid synthesis in muscle and adipose tissues. The SREBF1 gene, its regulating genes SCAP and MBTPS1, and one of its target genes, SCD, were investigated for associations with several meat quality traits of San Daniele hams. Significant associations of some gene markers were found with carcass weight, lean percentage, backfat thickness, ham green weight, ham fat cover thickness, shear force (WBSF), salting losses and instrumental colour of both lean and fat. These findings provide initial evidences that SNPs in SREBF1, SCAP, MBTPS1 and SCD are associated with San Daniele ham quality and may be considered as markers for selective breeding programs. [Copyright &y& Elsevier]

Abstract: The aim of this study was to investigate the effect of Montbéliarde (Mb) gene frequency on fatty acid composition and sensory properties of Italian Simmental (IS) steaks (longissimus thoracis m.). Twenty-seven bulls belonging to three strains with different percentages of Mb genes: traditional (ISt), without Mb ascendants (ISt=0% Mb genes), cross-strain (ISmt=37.5–50% Mb genes), Montbéliarde strain (ISm=87.5–100% Mb genes) and balanced for stearoyl Co-A desaturase genotype were considered. ISt has the highest C20:4 n−6 (P <0.01), C22:4 n−6 (P <0.05) and total PUFA n−3 level (P <0.01), while ISt and ISmt have higher C18:3 n−3 (P <0.05) and slightly lower MUFA (P =0.08) than ISm. Sensory tests indicated that the three experimental groups can be differentiated; moreover, ISmt meat is perceived as less hard (P <0.01), less chewable (P <0.01) and less fibrous (P <0.05) than ISt meat. [Copyright &y& Elsevier]

The effectiveness of the analysis of stable isotope ratios (13C/12C and 15N/14N) in fractions of lamb meat, measured by isotope ratio mass spectrometry, was evaluated as a method of feeding and geographical origin authentication. Analyses were carried out on meat from 12 lamb types, produced in couples in six European countries (country of origin, CO) and divided in three groups according to the feeding regime during their finishing period: suckled milk only, pasture without any solid supplementation and supplementation containing maize grain (feeding regime, FR). These analyses were made on two samples of longissimus thoracis muscle, taken from the 13th rib section of the left side of two different lambs, randomly chosen between the 120 selected to represent each lamb type. δ13C values varied significantly in different meat fractions, the difference being higher in protein than in fat (average difference 5.0‰). However, the pairs δ13C values of crude fat and protein were highly correlated (r=0.976) and affected by lamb type in a similar fashion, mainly reflecting animals’ feeding regime. Even δ15N values of meat protein fraction showed significant differences between lamb types, not dependant on the feeding regime. In fact, lambs fed on similar diets, but in different countries, gave meat with different 15N relative abundances. These findings provide the possibility of discriminating lamb types within the same feeding regime. Canonical discriminant analysis was carried out to evaluate whether lamb meat from different CO or FR or CO×FR interaction could be mathematically distinguished by its stable isotope ratios. On the basis of CO, the corrected empirical allocation of 79.2% of the initial observations and the corrected cross-validation of two thirds of the individual meat samples was obtained. FR gave better results: 91.7% of the individual meat samples was both correctly allocated and cross-validated, indicating the high potential of stable isotope ratio analysis as a tool for lamb diet characterisation. The most satisfactory classification attained, using K-means clustering technique and canonical discriminant analysis, enabled a good resolution of six CO×FR groups of lamb types: Icelandic fed on pasture; British and French grazing; Italian; suckled and Karagouniko concentrates finished; French Lacaune; Ternasco de Aragon. It was concluded that multielement stable isotope analysis may be considered promising for the reliable evaluation of lamb meat authenticity, in the same way as for wine, fruit juice, honey and dairy products. [Copyright &y& Elsevier]

Abstract: The aim was to investigate the effect of the genetic polymorphisms of leptin (LEP) and stearoyl-CoA desaturase (SCD1) genes on the fatty acid (FA) composition of the muscle of 103 Simmental bulls. Ten single nucleotide polymorphisms (SNP) were detected in exons 2 and 3 of the LEP gene, two of them encoding non-synonymous mutations. Allelic substitution effects of all the SNP on 28 single fatty acids, monounsaturated (MUFA) and polyunsaturated (PUFA) and desaturation indexes were estimated. Both the SCD1 SNP, as well as three SNP of the leptin gene, affected, to different extents, the desaturation of FA into MUFA. Because it was previously proposed that leptin''s metabolic action involves down-regulation of SCD1, it is possible that, beyond the mere additive effect of SCD1 gene on FA desaturation, the non-synonymous mutations in the leptin gene also contribute to the variability of FA composition in muscle fat. [Copyright &y& Elsevier]

Abstract: The present study was aimed to assess the effect of information about organic production on beef liking and consumer willingness to pay. Mean scores of perceived liking were higher for organic beef (OB) as compared to conventional beef (CB). Expected liking scores were higher for OB than for CB. For OB the expected liking was significantly higher than the perceived liking expressed in blind conditions (negative disconfirmation), whereas for CB no difference was observed. Consumers completely assimilated their liking for OB in the direction of expectations. Consumers showed a willingness to pay for OB higher than the suggested price (P <0.001), the latter corresponding to the local commercial value for organic beef. We conclude that the information about organic farming can be a major determinant of beef liking, thus providing a potential tool for meat differentiation to traditional farms. [Copyright &y& Elsevier]

The aim of the present study is to explore the association between food neophobia and chemosensory responsiveness and to determine whether this association translates into different food liking and preference patterns. Data were collected on 1225 respondents (61% females, age 20–60 years) as part of the Italian Taste project. Respondents completed the Food Neophobia Scale (FNS) as well as a food preference and familiarity questionnaire for a number of foods and beverages categorized as mild or strong tasting. Moreover, they evaluated attribute intensity and liking of an actual food (dark chocolate pudding) varying in the level of sweetness, bitterness and astringency. Taste function was evaluated by measuring fungiform papillae density (FPD), responsiveness to PROP (6-n-propylthiouracil) and to water solutions representing various oro-sensory qualities. High, medium and low neophobic subjects did not differ for FPD and chemosensory responsiveness. Reported liking was significantly lower for high neophobics than low neophobics mainly for those vegetables and beverages characterized by high levels of warning stimuli (i.e. bitterness, sourness, astringency and alcohol), whereas almost no differences were found for the bland versions of food items. High and medium neophobics rated astringency and, to a lesser extent, bitterness of the dark chocolate pudding, as more intense than low neophobics and liked the most bitter and astringent variants significantly less than low neophobics. Differences in liking, however, do not seem to be mediated by high food neophobics’ superior taste functioning but rather by higher levels of arousal when eating food and/or drinking beverages that are perceived as unpleasant and potentially dangerous. Finally, the effect of food neophobia was evident not only for unusual items in the Italian food context, but even for items that might be considered highly familiar. [ABSTRACT FROM AUTHOR]

Dietary and organoleptic qualities of pork products are largely influenced by the profiles of fatty acids (FAs) of meat and fat. The objective of this work was to investigate the potential associations between FA profile in subcutaneous adipose (back fat and leg fat) and muscular ( longissimus dorsi and biceps femoris ) tissues in heavy pigs (n = 129, 9-month-old) with single nucleotide polymorphisms (SNPs) in six candidate genes involved in fat metabolism: Stearoyl-CoA desaturase ( SCD ), Diacylglycerol acyltransferase 1 and 2 ( DGAT1 & DGAT2 ), Microsomal Triglyceride Transfer Protein ( MTTP ), Fatty Acid Synthase ( FASN ) and Heart- fatty acid binding protein ( H- FABP). Preliminary results suggest a putative association between MTTP , DGAT2 and FASN and the FA content in both fat and meat, whereas between DGAT1 , SCD and H-FABP the association was found in adipose tissue only. However, the effect of the analyzed genes, needs to be verified in a larger and better characterized pig population to support the hypothesized associations with FA content. [ABSTRACT FROM AUTHOR]

High meat quality is required for dry-cured ham production, which quality depends on meat fat quantity and composition. The aim was to study the polymorphisms of six genes involved in fat metabolism, namely, Stearoyl-CoA desaturase ( SCD ), Diacylglycerol acyltransferase 1 and 2 ( DGAT1 & DGAT2 ), Microsomal triglyceride transfer protein ( MTTP ), Fatty acid synthase ( FASN ) and Heart fatty acid binding protein ( H-FABP ) in two traditional (Large White or Duroc × (Landrace × Large White)) and two industrial hybrids (Goland and Danbred), which are used for dry-cured ham production. Significant associations of SCD and MTTP were found with carcass weight. DGAT2 was associated with back fat thickness and L* fat colour (objective white colour score). Several genes ( DGAT2 , MTTP & FASN ) were associated with weight loss during salting, first step in dry-cured ham production, affecting final yield. Finally, MTTP was associated with shear force. Our findings suggest that the SCD , DGAT2 , MTTP and FASN polymorphisms are associated with quality of heavy pig meat products. [ABSTRACT FROM AUTHOR]

The aim of this study was to analyze the volatile compounds, physicochemical characteristics, and sensory properties of Montasio, a semicooked pressed cheese, produced from the milk of the dual-purpose Italian Simmental cows grazing on alpine pastures. A total of 72 cows grazing on 2 pastures, which differed in botanical composition (nutrient-rich pasture vs. nutrient-poor pasture), received 2 different levels of supplementation (3.0 vs 1.5 kg/head per day). The experimental cheeses were produced from whole, raw milk and ripened for 60 d. Sixty-one volatile compounds, including alcohols (11), aldehydes (6), ketones (10), lactones (2), esters (6), hydrocarbons (3), carboxylic acids (6), phenolic compounds (4), monoterpenes (7), sesquiterpenes (1), sulfur compounds (4), and amines (1), were detected. The main families in terms of relative weight appeared to be carboxylic acids, esters, and alcohols. A panel of trained assessors described the experimental cheeses as having an intense color; small and evenly distributed eyes; an intense odor and flavor of milk-sour, milk, and cow; and a tender and creamy texture. The pasture type affected the volatile fraction, particularly ketones, phenolic compounds, and terpenes, which are overall higher in nutrient-poor pastures. A slight effect on the sensory analyses, in particular the effect of the cow attribute on odor and flavor, was perceived by the panelists. The cheeses produced on nutrient-rich pasture had higher b* (yellowness) index. These results were consistent with the color evaluation of the sensory panel. In addition, the pasture affected some textural attributes (adhesivity, creaminess, and granules) as perceived by the panelists. Concentrate supplementation, which is required to meet the feeding requirements of grazing cows, had no clear effect on either the volatile compounds or the sensory properties of the cheeses. Thus, at least within levels of integration adopted, it is expected not to alter the organoleptic characteristics of this product. [ABSTRACT FROM AUTHOR]

Abstract: Heavy pigs are used in Italy to produce fresh meat, dry-cured ham, salami and lard. Fatty acid profile determines both the dietary and organoleptic qualities of these products. The objective was to study the polymorphisms of two genes that code for enzymes of the fatty acid metabolism, namely, Stearoyl-CoA desaturase (SCD) and Fatty acid desaturase 2 (FADS2). We also investigated the polymorphism of the Sterol regulatory element binding protein gene (SREBF1) as it regulates SCD and FADS2 transcription. Significant associations of SCD were found with the ratio of oleic to stearic acid; with the concentration (g/100g of fat) of stearic and oleic acids. The concentrations of arachidonic and linoleic acids and the ratio of dihomo-gamma-linolenic to linoleic acid were significantly associated with FADS2 polymorphisms. SREBF1 polymorphism was associated with oleic acid concentrations and the ratio of arachidonic to linoleic acids. Our findings suggest that the SCD, FADS2 and SREBF1 polymorphisms are associated with dietary quality of heavy pig meat products. [Copyright &y& Elsevier]

Conventional chicken from a fast-growing strain (CC), organic chicken from a slow-growing strain (OSG), and organic chicken from a fast-growing strain (OFG) were used to assess descriptive sensory differences between organic and conventional breasts, to verify whether differences were perceived by consumers and to evaluate the effect of information about organic production on liking. A conventional quantitative-descriptive analysis was performed by a trained panel of 10 members on breast slices (1 cm thick) grilled at 300±C. A 150-member consumer panel (from southern, central, and northern Italy) rated CC, OSG, and OFG breasts according to 3 types of evaluation: tasting without information (perceived liking), information without tasting (expected liking), and tasting with information (actual liking). Breasts from different sources were clearly discriminated by the trained panel as meat from CC was perceived more tender than OFG (P <0.05) and OSG (P<0.001), more fibrous than OFG (P<0.05) and OSG (P< 0.001), and leaving more residue than OFG (P<0.05) and OSG (P<0.001), whereas OSG was assessed as less juicy before swallowing than OFG and CC (P<0.05) and less fibrous than OFG (P<0.05). No significant differences were observed by consumers for perceived liking. However, consumer expected liking scores were higher for organic than for conventional products (P<0.001) and actual liking of organic breasts moved toward the expectancy. In particular, actual liking scores were higher than perceived liking in blind conditions (P<0.001 and P<0.01 for OFG and OSG, respectively). We conclude that trained panelists were able to discriminate chicken breasts from different sources, whereas untrained consumers were not. However, consumer liking was markedly affected by the information given on the organic production system, thus providing a tool to differentiate the product in an increasingly competitive market. [ABSTRACT FROM AUTHOR]

Abstract: The effect of long term dietary linseed addition on performance, carcass characteristics and meat quality of Italian Simmental (IS) and Italian Holstein (HI) young bulls was investigated. Thirty-two animals were assigned to 4 groups following a factorial design: 2 breeds – IS and IH – ×2 diets – containing whole ground linseed (5–8% of DM) and control. IS had greater in vivo performance and carcass characteristics than IH. IS muscle had lower C14:0, C16:0, SFA, higher C18:2n-6 cis, PUFAn-6, PUFA and PUFA/SFA proportion than IH in phospholipids (PL) fraction. Linseed inclusion did not affect animal''s performance and carcass characteristics. In muscle PL, linseed increased C20:0, C22:0, C23:0, C20:5n-3 and decreased C20:4n-6, PUFAn-6/n-3, PUFAn-6 concentration. Linseed decreased C14:0, C16:0 proportion in neutral lipids (NL) and increased total PUFAn-3, C18:3n-3 proportions both in NL and PL fraction. However, these differences were relatively low from a quantitative point of view. [Copyright &y& Elsevier]

Abstract: This paper describes the responses of consumers in six European countries (Greece, Italy, Spain, France, Iceland and United Kingdom) tasting meat from twelve different local types of lambs produced in those same six countries. Animals represented 10 breeds and crossbreeds, three sexes, several diets composed of either milk, concentrates and various forages as main ingredients and different slaughter ages, from 1 and 12 months, and carcass weights, from 5.5 to 30.4kg. Tests were conducted by 36 volunteer families in each of the six countries involved in the study. Families were asked to roast the joints using their own cooking criteria, evaluating (from “dislike extremely” to “like extremely”) flavour, tenderness, juiciness and overall liking. Also the cook was asked to rate the odour during cooking. Country and lamb type and their interaction were statistically significant for all the variables analysed. Results suggest a link between the assessments of a given lamb type and the consumers’ culinary background, showing clear associations between country and lamb type preferences. It was possible to separate, independently of the country, different groups of families with similar preferences. Five family groups, which included 88 families (40.74%), had a clear Mediterranean origin and preferred types of lamb fed either milk or mainly concentrate diets. Seven family groups, which included 93 families (43.06%) with a clear northern origin, preferred types reared on grass or with grass included in the diet. The rest of the groups (four) that included 35 families (16.20%) had no clear composition (northern or Mediterranean), and they had a wider taste preference. It can clearly be seen that there are two categories of consumers of lamb in the analysed European market: those who prefer “milk or concentrate taste” and those who prefer “grass taste”. [Copyright &y& Elsevier]

Abstract: Calpain system (μ- and m-calpain, calpastatin) and myofibril fragmentation index (MFI) were analysed on muscle longissimus thoracis et lumborum of 16 male fallow deer, half 18- and half 30-month-old, slaughtered after a 4-month feeding trial on pasture alone or with concentrate supplement. Diet did not produce any difference on the measured variables. The muscle of the elder deer had a higher calpastatin level 6 h post-mortem (136.0 vs. 99.7 units/50 g, for the 30- and 18-months-old, respectively), and a lower MFI value, particularly 24 h post-mortem (71.1 vs. 89.1). This is in agreement with previous data showing tougher meat from 30-month-old animals. [Copyright &y& Elsevier]

Thirty-two male fallow deer, half 18- and half 30-month-old, were slaughtered after a 4-month feeding trial on pasture alone or with daily supplement of 500 g (dry matter) concentrate/head (eight each 18- and 30-month-old). Neither diet nor age produced significant effects on M. longissimus thoracis et lumborum (LM) and on M. semimembranosus (SM) pH and colour. Meat texture properties (Warner–Bratzler–WB Shear Force and compression), water loss and collagen content were unaffected by diet, while the older deer had higher LM WB peak force values (4.15 vs 4.88 kg) and lower collagen solubility (34.61 vs 22.58%). LM and M. semitendinosus (ST) of the supplemented deer had a higher content of fat (pasture vs concentrate feeding: 0.56 vs 0.72% in LM; 0.55 vs 0.78% in ST) and provided lower PUFA, particularly n-3 PUFA, and higher n-6/n-3 ratio (3.30 vs 4.76 in LM; 3.39 vs 4.63 in ST). Thirty-month-olds’ LM and ST were fatter than 18-month-olds’, and provided lower PUFA, both in the n-6 and n-3 fraction. [Copyright &y& Elsevier]

• Biodynamic and conventional wine were tested blind and labeled. • In blind condition, no differences in liking or willingness to pay were observed. • Pleasantness increased with information about biodynamic wine. • Assimilation of information for biodynamic wine was incomplete. • Information did not affect pleasantness for conventional wine. [ABSTRACT FROM AUTHOR]

• Individuals greatly vary in fungiform papillae pattern (density and size) on the tongue. • The FP density decreases in all diameter sizes with aging. • Limited gender effects were found on FP density for specific diameter sizes. • No association was found between PROP status and FP densities in different diameter sizes. • Uniform and small size FP were associated with increased responsiveness to oral stimuli. The association of fungiform papillae (FP) density with responsiveness to oral sensations is controversial. It has been speculated that FP size is a relevant feature for oral responsiveness, thus partially accounting for inconsistencies between FP density and oral responsiveness. This study aims to evaluate factors affecting the number of FP with different diameter sizes (age, gender, PROP status) and to explore the relevant associations with responsiveness to oral sensations in water solutions and food products. Three-hundred-fifty subjects participating in the Italian Taste project were involved, balanced for gender (53% females) and age class (18–30, 35%; 31–45, 35%; 46–60, 30%). The automated image analysis of tongue pictures was used to detect FP and count their frequencies, distinguishing 11 diameter size classes (range from 0.3 to 1.05 mm). Frequencies were converted into FP densities (FP/cm2) for each size. Taste responsiveness in water solutions (sour, bitter, salty, umami, sweet, astringent, pungent) and in three food products with varied intensity of target sensations (pear juice for sourness; bean purée for saltiness; tomato juice for pungency) was measured. Density of FP from all size classes decreased with age. Females showed higher FP density in size of 0.78–0.84 mm diameter, and males in the size of 0.44–0.49 mm. PROP status did not significantly affect the density of FP in any size class. Principal Component Analysis on individual FP density values of the 11 classes allowed identifying four subject groups with different FP patterns: high density and large diameter (HighLarge), high density and small diameter (HighSmall), low density and large diameter (LowLarge), low density and small diameter (LowSmall). FP pattern groups significantly affected oral responsiveness, the highest responsiveness was associated to the uniform pattern characterized by low density and small size FP. [ABSTRACT FROM AUTHOR]