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In this study, hydrogen production via microwave plasma is investigated, analyzed and simulated in a novel way for practical applications. The water vapor when in proximity of a tungsten electrode is modeled for the generation of hydrogen gas. A numerical simulation study is performed using plasma and electromagnetic wave COMSOL modules to analyze the plasmolysis of water vapor within a vacuum concealed reaction vessel entailing a tungsten electrode. A kinetic model is therefore developed to represent the reaction mechanisms and interactions between the species within the plasmolysis reactor. The dynamic results of electron density, electron temperature, plasma rate, and species interactions are extracted through the kinetic model. Within the time domain of 10−16 to 10−14 s, the hydrogen concentration is found to increase to 4.5815 × 10−11 mol/m3 with a corresponding decrease in water vapor concentration of 1.782 × 10−8 mol/m3, respectively. The dynamic variations in the concentrations of other dissociated species are investigated across the geometry of the reaction domain studied, and it is therefore concluded that the tip of the electrode entails the highest species concentrations. • Modeling of microwave hydrogen production via water vapor in COMSOL Multiphysics. • Plasma and electromagnetic frequency domain COMSOL modules utilized. • Hydrogen concentration of 4.5815 × 10−11 mol/m3 achieved within 10−16 to 10−14 s. • Corresponding water vapor concentration reduces by 1.782 × 10−8 mol/m3. [ABSTRACT FROM AUTHOR]

While it is well known that the nature of the applied electric field and the heterogeneity of the tissue can influence the impact of PEF treatment on the plant tissues found in plant-based foods, few studies have investigated the influence of PEF on plant structures that are made up of multiple structurally similar organs. The aim of this study was to understand the effect of pulsed electric fields (PEF), at different electric field strengths (0, 0.3, 0.7 and 1.2 kV/cm) and specific energy (7, 21 and 52 kJ/kg), on a multilayered plant material, with bunching onion bulb tissues being used as a model system. The present study found that carbohydrates leakage was an appropriate index to assess PEF induced damage and that plasmolysis of epidermal cells was a good indicator of plasma membrane integrity after PEF. In addition, electric field strength had a greater impact on the cell integrity than specific energy applied. While other studies have shown that different cell types have different sensitivities to PEF, using plasmolysis as an indicator of cell damage, this study clearly showed that the same PEF treatment conditions had a greater effect on the epidermal cells of the outer scales compared to the inner scales. Hence, while different plant cell types vary in their sensitivities to PEF the spatial location of the same cell type within a complex plant material made up of multiple similar organs, i.e. an onion bulb, can also influence how cells respond to the PEF treatment. Despite PEF induced disruption at the cellular level being detected by carbohydrate leakage, the epidermal cell plasmolysis test and by cryo-scanning electromicroscopy (cryo-SEM), no gross structural changes at the organ level were observed using cryo-SEM or fluorescence microscopy. This study also reports for the first time that PEF treatment can enhance fructan leakage from onion bulbs, which means that PEF treatments have the potential to manipulate the fructan contents of some plant-based foods. Unlabelled Image • Electric field strength had a greater impact on cell membrane permeability than specific energy input. • The effect of PEF treatment on epidermal cells increased from the outer to inner scale leaves. • The effect of PEF on the same tissue type depended on the location of the tissue within the same bulb. • Increasing the electric field strength increased fructan leakage. [ABSTRACT FROM AUTHOR]

It was recently suggested to end the usage of the term cell wall in microbiology (including prokaryotes and fungi) because it represented an inappropriate and potentially misleading metaphor (Casadevall and Gow, 2022). The analysis of the arguments for such a move from the viewpoint of the plant physiologist indicates that the suggestion is based on misunderstandings, first, of the early history of cell biology and its terminology; second, of the development of modern concepts of cell wall function since the late 19th century; and third, of the nature of metaphors and their role in scientific communication. We conclude that misconceptions concerning cell walls may arise due to pedagogical shortcomings in introducing students to our technical terminology rather than from the fact that part of this terminology originated from metaphors. [ABSTRACT FROM AUTHOR]

Natural nanoscale polysaccharide and its application have attracted much attention in recent years. In this study, we report for the first time that a novel naturally occurring capsular polysaccharide (CPS-605) from Lactobacillus plantarum LCC-605, which can self-assemble into spherical nanoparticles with an average diameter of 65.7 nm. To endow CPS-605 with more functionalities, we develop amikacin-functionalized capsular polysaccharide (CPS) nanoparticles (termed CPS-AM NPs) with improved antibacterial and antibiofilm activities against both Escherichia coli and Pseudomonas aeruginosa. They also exhibit faster bactericidal activity than AM alone. The high local positive charge density of CPS-AM NPs facilitates the interaction between the NPs and bacteria, leading to extraordinary bactericidal efficiencies (99.9 % and 100 % for E. coli and P. aeruginosa, respectively, within 30 min) by damaging the cell wall. Very interestingly, CPS-AM NPs exhibit an unconventional antibacterial mechanism against P. aeruginosa , that is, they can induce plasmolysis, along with bacterial cell surface disruption, cell inclusion release and cell death. In addition, CPS-AM NPs exhibit low cytotoxicity and negligible hemolytic activity, showing excellent biocompatibility. The CPS-AM NPs provide a new strategy for the design of next-generation antimicrobial agents that can reduce the working concentration of antibiotics to fight against bacterial resistance. • A novel capsule polysaccharide (CPS-605) is obtained from Lactobacillus plantarum LCC-605. • AM is successfully conjugated with CPS-605 to generate CPS-AM NPs. • CPS-AM NPs significantly reduce the working concentration of amikacin. • CPS-AM NPs exhibit excellent antibacterial activity against Gram-negative strains. • CPS-AM NPs show great biofilm inhibition and disruption abilities. [ABSTRACT FROM AUTHOR]

Numerous prokaryotes accumulate polyhydroxybutyrate (PHB) intracellularly as a storage material. It has also been proposed that PHB accumulation improves bacterial stress resistance. Cupriavidus necator and its PHB non-accumulating mutant were employed to investigate the protective role of PHB under hypertonic conditions. The presence of PHB granules enhanced survival of the bacteria after exposure to hypertonic conditions. Surprisingly, when coping with such conditions, the bacteria did not utilize PHB to harvest carbon or energy, suggesting that, in the osmotic upshock of C. necator , the protective mechanism of PHB granules is not associated with their hydrolysis. The presence of PHB granules influenced the overall properties of the cells, since challenged PHB-free cells underwent massive plasmolysis accompanied by damage to the cell membrane and the leakage of cytoplasm content, while no such effects were observed in PHB containing bacteria. Moreover, PHB granules demonstrated “liquid-like” properties indicating that they can partially repair and stabilize cell membranes by plugging small gaps formed during plasmolysis. In addition, the level of dehydration and changes in intracellular pH in osmotically challenged cells were less pronounced for PHB-containing cultures, demonstrating the important role of PHB for bacterial survival under hyperosmotic conditions. [ABSTRACT FROM AUTHOR]

This work analyzes the effect of Pulsed Electric Fields (PEF) as a pre-treatment of the osmotic dehydration (OD) of kiwifruit ( Actinidia deliciosa cv Hayward ) in the internal structure and in the internal water transport. PEF pre-treatments were done using three PEF intensities (100, 250 and 400 V/cm) and analyzed by TD-NMR. The OD was carried out by immersing the samples in 61.5% sucrose solution at 25 °C. The application of a PEF pre-treatment before the OD produces a process of plasmolysis proportional to the electric field applied. It is because the PEF removes the mobile charges of the medium, such as electrolytes, organic acids, amino acids; Ca + 2 is the major culprit of the plasmolysis because it fixes some of the junctions of the microtubules between the cell wall and the membrane. Therefore, a previous plasmolysis produces an increase in the apoplastic transport increasing the rate of dehydration. Industrial relevance This research describes the effect of the PEF pre-treatment of osmotic dehydration in the microstructure and in the internal water liquid phases transports of the kiwifruit tissue by using TD-NMR. The results of this research have demonstrated that the application of an electric field prior to the osmotic dehydration produces a process of plasmolysis more the greater the electric field applied, due to the elimination of the mobile charges of the medium and mainly affecting the internal transports. Therefore, PEF pre-treatment accelerate the OD treatment preserving the structure and consequently remaining the life cycles, necessaries to obtain a long shelf life of the product. It has been demonstrated the useful of the TD-NMR as a technique able to analyzed the effect of PEF at a microstructural degree. [ABSTRACT FROM AUTHOR]

Plasma technology is gaining increased interest for the reduction of carbon dioxide (CO 2 ) to carbon monoxide (CO) and oxygen (O 2 ). CO is a reaction partner with hydrogen (H 2 ) to produce gas and fuel. This paper elaborates an improved method to produce methane (CH 4 ) by plasma induced dissociation of carbon dioxide and H 2 in one single setup. A low pressure glow discharge plasma with an integrated zirconia based ion conductor transports oxygen ions (O 2− ) in or out of the reaction chamber. Transporting oxygen ions into the reaction chamber is a way to have a controlled amount of water vapor for the conducted experiments. In contrast, leads the reduced amount of O 2 ions to a theoretical decrease in the recombination of CO and O 2 to CO 2 in the ionized gas and therefore increases the CO 2 conversion rate. The applied voltage to the ion conductor for the outward ion transportation and the power of the plasma source current have a major impact on the amount of extracted O 2− ions. The effect of these parameters on the CO production rate and the formation of methane are investigated. A positive effect of the plasma current on the amount of CO and CH 4 is detected. The influence of the temperature increase from the heated ion conductor relates to an undesired increase of the recombination rate of CO and O 2 to CO 2 , but has a positive effect on the methanation. [ABSTRACT FROM AUTHOR]

[Display omitted] • Microplasmas can reduce energy requirements of water plasmolysis on par with electrolysis. • Hydrogen producing technologies could produce maximum 25-30 g/kWh energy yield. • The optimized packed bed microplasma reactor in current study produces 23.9 g/kWh energy yield. • Microplasma reactor produces 16.31% more hydrogen energy yield in comparison to electrolysis. • Microplasma reactor can be used for decentralized power system for rural areas. Hydrogen as secondary energy carrier is one of the promising routes to meet renewable and sustainable energy demand. Hydrogen production using water plasmolysis, so far, has been considered as energy intensive. However, recent developments in the field of microplasmas has reduced energy requirement of water plasmolysis on par with electrolysis. However, there's still room for improvement as water vapors dissociation efficiencies are less than thermodynamic and kinetic limits. One of the ways to do that is to split microchannel in plasma into sub-micron channels by packing beads inside the reactor. The current study investigates the plasmolysis of water vapours (steam) and argon gas with (1) empty channel plasma reactor and (2) glass beads packed plasma reactor. A custom-made Corona-DBD hybrid microplasma reactor has been designed and employed for water steam plasmolysis. This study aims to investigate the feasibility of water vapors dissociating into hydrogen at a relatively small interelectrode gap at atmospheric pressure. The minimum breakdown voltage has been found to be 3.7 kV pk-pk at 5 mA current. The maximum hydrogen production rate was observed 23.9 g/kWh at optimized conditions for the glass beads packed plasma reactor. So, in current study glass beads packed plasma microreactor yielded 23.9g of H 2 /kWh which is significantly higher than any previously published study [ABSTRACT FROM AUTHOR]

Some species of the genus Passiflora (sect. Xerogona ) have flowers that exhibit a mechanically-induced movement of the androgynophore that is probably involved in pollination. Despite the many reports of touch-induced movements of floral parts, the studies concerning anatomical, ultrastructural and molecular aspects of the plant movements are restricted to the vegetative parts. Rapid plant movements are highly dependent on turgor changes of a particular flexible tissue formed by specialized cells capable of losing and gaining water rapidly. Thigmotropic androgynophores of four species of Passiflora from section Xerogona were analyzed at cellular and subcellular levels. Our results show that the movement is due to a vacuolar remodelling in a group of parenchymatous cells at the base of the androgynophore. After the movement, plasmolyzed and multivacuolated cells are present at the stimulated side and turgid and univacuolated cells at the opposite side. The results suggest that the mechanisms leading to the androgynophore movement in Passiflora are, in general, analogous to those reported for the movements of legume pulvinus, motile stamen filaments and guard cells of stomata, pointing to conserved cellular mechanisms of plant movement. [ABSTRACT FROM AUTHOR]

[Display omitted] • Hydrogen production pathways via renewable and non-renewable sources. • Renewable energy sources gaining potential as clean energy source to produce H 2. • Plasmolysis is competitive to electrolysis for H 2 production. • Plasmolysis is beneficial for its low power consumption and reduced equipment size. • Solar integrated plasmolysis with zero carbon footprints is the proposed strategy. Hydrogen as an energy carrier can provide a long term solution to the problem of sustainable supply of cleaner and environmentally friendly fuel. Hydrogen finds extensive use s in petroleum, chemical synthesis and treated as a zero-emission fuel for transportation as well. It could also be used to produce power. Especially, in the remote areas away from main cities where electrification cost would be significantly higher. A hydrogen based decenteralized system could be developed where the "surplus" power generated by a renewable source could be stored as chemical energy in the form of hydrogen. 80% of the whole hydrogen produced is by steam methan reforming at an energy efficiency of 74–85%. However, steam methane reforming and other fossil fuel based technologies are neither green nor sustainable. Hydrogen, could only be counted as a renewable and clean fuel if the required power to produce hydrogen comes from a renewable source such as wind or solar power. Using a renewable source, hydrogen could be produced by electrolysis, biohydrogen, thermochemical cycles, photocatalysis, and plasmolysis. Amongst hydrogen production technologies, electrolysis contributes the highest 4% of the total world's energy demand. The production cost and energy efficiency estimated for electrolysis are 10.3 $/kg and 52%, respectively. Electrolysis, an energy-intensive process for hydrogen production, is still confronting challenges to manifest itself economically. While, the production rate of 20 g/kWh with predicated cost and efficiency 0.09 €/kWh or 6.36 $/kg and 79.2%, respectively, has been reported that depicts plasmolysis competitive on par with electrolysis with the advantage of low power consumption, reduced equipment size and principle cost. This review highlights the current status, potential, and challenges of both renewable and non-renewable hydrogen production. A new strategy for simultaneous hydrogen production and separation by microplasmas and microbubble mediated mass transfer has been proposed. A decenterlaized system for hydrogen generation by combining the proposed strategy with solar energy has been suggested to reduce the carbon footprints. [ABSTRACT FROM AUTHOR]

Copyright of Revista Iberoamericana de Micologia is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Revista Iberoamericana de Micologia is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Abstract: Curcumin was successfully encapsulated in yeast cells of Saccharomyces cerevisiae as confirmed by fluorescence microscopy, Differential Scanning Calorimetry (DSC) and Fourier Transform Infrared Spectroscopy (FT-IR). Emphasis was given on the encapsulation parameters including temperature, plasmolysis of yeast cells, presence of ethanol and mass ratio curcumin:cells that affected the amount of curcumin finally encapsulated, as expressed by the %Encapsulation Yield (%EY) and %Encapsulation Efficiency (%EE). Encapsulation was favoured at temperatures above 35°C and preparation of microcapsules in water instead of 50% v/v ethanol increased the %EY and %EE values by at least 2-fold. Although plasmolysis of yeast cells modified membrane’s fluidity and cell wall’s composition, the microcapsules prepared with plasmolysed cells did not differ in their curcumin content when compared to those prepared with non-plasmolysed cells. Proper combination of the abovementioned parameters resulted in microcapsules that contained up to 35.8±0.86% w/w curcumin. In all microcapsules prepared curcumin was integrated in the plasma membrane bilayer but also interacted with constituents of the cell wall network. [Copyright &y& Elsevier]

Surplus renewable electricity used to convert CO 2 into CO, the building block of liquid fuels, advances the energy transition by enabling large-scale, long-term energy storage and the synthesis of fuel for long-haul transportation. Among the various technologies developed, renewable electricity driven conversion of CO 2 by high-temperature electrolysis and by plasmolysis offer a tantalising potential. High-temperature electrolysis is characterized by high-yield and energy-efficiency and the direct separation of the CO 2 dissociation products CO and O 2. However, the difficulty to break the carbon-oxygen double bond poses challenging requirements on electrode materials. CO 2 plasmolysis on the other hand, offers a similar energy efficiency, does not employ scarce materials, is easy to upscale, but requires efficient gas separation and recuperation because the produced CO remains mixed with O 2 and residual CO 2. Here, we demonstrate that the coupling of the two processes leads to a renewable-electricity-driven route for producing CO from CO 2 , overcoming the main bottleneck of CO 2 plasmolysis. A simulated CO 2 plasmolysis gas mixture is supplied to a high-temperature electrolyser to separate the product gases electrochemically. Our results show that the product stream of the coupled-process contains 91% less oxygen and 138% more CO compared with the bare plasmolysis process. Apart from upgrading the produced gas mixture, this coupled approach benefits from material stability. Durability tests (~100 h) show better stability in coupled operation when compared with conventional CO 2 electrolysis. Synergy between plasmolysis and electrolysis opens up a novel route to efficient CO 2 conversion into valuable CO feedstock for the synthesis of long-chain hydrocarbons. • CO 2 conversion via coupled plasma-electrolysis process is demonstrated. • CO 2 plasmolysis gas mixture was upgraded by electrochemical oxygen separation. • Coupled-process outlet contains 91% less O 2 and 138% more CO vs bare plasmolysis. • Better stability was achieved in coupled operation vs conventional CO 2 electrolysis. • Synergy between plasmolysis and electrolysis opens up a novel CO 2 conversion route. [ABSTRACT FROM AUTHOR]

Abstract: The influence of pulsed electric fields (PEF) and heating on the cell damage (plasmolysis) of sugarbeet tissue was studied. The PEF treatment was applied using bipolar pulses of near-rectangular shape in near-isothermal regime. The thermal treatment temperature interval was within T =20–70°C. The tissue damage degree was characterized by the electrical conductivity disintegration index Z. The Arrhenius form of the characteristic damage time – temperature dependences was observed both for the electrical and thermal processes. The corresponding activation energies W ≈149–166kJmol−1 were responsible for the damage of cell membranes. The PEF treatment noticeably accelerates the diffusion process, which requires ≈1h with mild heating at 40°C. The results shown open new prospects for low heating processing in sugar industry. [Copyright &y& Elsevier]

Abstract: The pulsed electric fields (PEF) enhanced expression of juice from slices of two different sizes was analysed for apple and carrot tissues. The experiments were carried out using a laboratory filter-press cell, equipped with PEF-treatment system operating at moderate electric field strength (250–400V/cm) and at constant pressure (P =5bar). In different experiments the PEF-treatment was applied at 30 and 1000s after the start of pressing. The expressed juice was collected in different containers and its mass, absorbance and soluble dry mater content (°Brix) were measured. It was shown that PEF-application for juice expression from soft plant tissues allows to enhance the juice yields and to regulate qualitative characteristics of the juice. Enhancing of the juice expression (Y) after PEF-application was accompanied with a noticeable decrease of absorbance (C) and increase of °Brix value of the juice for both apples and carrots. The average characteristics of the juice divided in two (initial and final) portions were analysed. The PEF-application allows to separate the second portion of juice with the lowest absorbance and highest °Brix values at yields ≈20–30% for apple and ≈20% for carrot. The time of PEF-application and the size of slices affected noticeably characteristics of both initial and final portions of the juice. [Copyright &y& Elsevier]

This paper reports an investigation of the release of organic and inorganic phosphorus from a riverine sediment subjected to salinity conditions typical of estuarine mixing. Freshwater sediment was mixed with filtered river water in a thermostatted reactor, and allowed to equilibrate under aerobic conditions for 3 days. Salinity was then increased in a stepwise manner by addition of filtered low-nutrient seawater over a period of 4 days. A control experiment was performed in a second reactor by substituting ultrapure water for seawater. Using a flow injection analysis method for measurement of filterable reactive phosphorous (FRP, the so-called inorganic fraction) and filterable organic phosphorous (FOP), it was found that rapid releases of both FOP and FRP occurred at salinities of ⩾10‰. Over the 4-day experimental period, sediment release increased the filterable P concentration by approx. 50 μg L−1, and of this, nearly half was initially present as FOP, which subsequently underwent rapid mineralisation to FRP. The observed behaviour may be explained by a combination of salinity induced plasmolysis of sediment bacteria and ion exchange by suspended sediment particles. [Copyright &y& Elsevier]

To test the hypothesis that xylogenesis is coupled to cell growth suppression, cell expansion in Zinnia elegans L. var. Envy mesophyll suspension cultures was manipulated by varying the extracellular osmolarity and the effect on xylogenesis was examined. Cell expansion and tracheary element differentiation were inversely related along a gradient of extracellular osmolarity ranging from 200 to 400 mOsm, supporting the hypothesis that tracheary element differentiation is coupled to cessation of cell expansion. Above 300 mOsm, reduction in the number of cells that differentiated into tracheary elements coincided with an increase in the number of plasmolyzed cells as extracellular osmolarity was increased, indicating that plasmolysis inhibits tracheary element differentiation, although not specifically. Using the plasmolysis method we showed that cellular osmolarity within populations of isolated Zinnia mesophyll cells ranges from 250 to 600 mOsm with a mean of 425 mOsm. The broad range in cellular osmolarity within Zinnia mesophyll cell populations, coupled with inhibition of differentiation in the low range due to cell expansion and in the high range due to plasmolysis, may help explain why tracheary element differentiation in Zinnia suspension cultures is never complete nor perfectly synchronous and enable further optimization of this culture system. [Copyright &y& Elsevier]

The expression behaviour of different soft vegetable tissues is compared. The detailed analysis of different consolidation stages is done for potato tissue. The primary consolidation Terzaghi’s coefficient Cv is estimated as Cv&ap;(2.0±0.6)×10−2 cm2/s. The logarithmic type of secondary consolidation is observed and the dependency of the consolidation processes on the applied pressure is discussed. The four main time constants that characterize the consolidation curve are defined: t0 is the time of pre-consolidation stage, t1 is the time of intensive liquid expression during the primary consolidation stage, t2 is the time of transition to the secondary consolidation, and td is the expected ‘theoretical’ time of complete liquid expression. At a pressure of P=5 bar, t0&ap;10 s, t1&ap;103 s, t2&ap;104 s, and td&ap;6×104 s (17 h). Consolidation behaviour after application of pulsed electric fields (PEF) is studied. At low degrees of PEF treatments (electric field intensity E<300 V/cm, total time of PEF treatment TPEF<0.1 s), unchanged pressing behaviour at the primary consolidation stage (t) and enhanced expression in the transitional time interval t1 was observed. It was concluded that substantial intensification of the pressing processes needs a rather high degree of PEF-induced tissue damage and low-level PEF-induced damages show notable effects only at large t>t1, which is disadvantageous from industrial point of view. It is shown that PEF should be applied after a pre-consolidation stage. But the time at which PEF treatment starts tPEF has no significant effect on the juice yield for the case when t0. An optimum value of PEF intensity E required for high intensification of juice expression at minimum electric power consumption lies in the interval of 400–600 V/cm. [Copyright &y& Elsevier]

The effect of the concentration of sucrose solutions on the cellular structure of potato tissue in equilibrium at 27 °C was studied. Two different methods of investigation were used to determine the volume of the different phases composing the cellular tissue of the potato when in equilibrium with the solutions, one based on data of the concentration itself and the overall volume of 2 mm slices after 48 h at equilibrium, and the other on microscopic images of cells in thin slices of fresh tissue stained with neutral red after an hour in equilibrium to show protoplasts, vacuoles and plasmolysis spaces. The results of these methods were compared with those obtained by a predictive thermodynamic approach considering the semipermeability of cell membranes. Phase volume data obtained from microscopic analysis were more similar to what was predicted by the theoretical model than those obtained by means of composition measurement, where the long equilibrium time apparently led to the loss of semipermeability of the cell membranes, since total volumes calculated without consideration of the cell membranes were similar to those measured. This suggests that the length of time of osmotic dehydration brings about a change in cell structure and the consequent involvement of a different mechanism in mass transfer. [ABSTRACT FROM AUTHOR]

[Display omitted] • Salt maceration and sugar addition resulted in plasmolysis of greengage skin cell. • Spontaneous fermentation was conducted by indigenous microbiota. • The genus of Pichia dominated the traditional fermentation of greengage wine. • Non- Saccharomyces yeast contributed to the evolution of terpenes and ethyl esters. • A bacteria genus of Gordonia correlated to the development of characteristic volatiles. Greengage wine is a popular drink in Southeast Asia. Salt maceration and sugar addition in traditional fermentation caused plasmolysis of greengage skin cell. In this case, the development of indigenous microbiota can use the nutrition of exosmosis of cell tissue fluid. The result of high-throughput sequencing technology indicated the non-Saccharomyces yeasts dominated the entire process of traditional fermentation. Key yeast genera, such as Gliocephalotrichum, Sordariales, Candida and Issatchenkia were identified, a dynamic non-Saccharomyces yeast community was spontaneously formed and highly correlated to the evolution of volatile compounds of greengage wine, such as monoterpenes, C13-norisoprenoids, ethyl esters and ethylphenols. Yeast glycosidases released nonvolatile aroma precursors into free form, which contributed to the aroma profile with strong flowery and fruity flavor in greengage wine. Moreover, a bacteria genus of Gordonia performed significant correlations to the development of characteristic volatiles at the beginning of primary fermentation. [ABSTRACT FROM AUTHOR]