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Large organizations respond to huge volumes of Request for Proposals (RFP)/ Request for Information (RFI) every year. The process of developing a proposal for an RFP/ RFI is completely manual and time-, effort-, and intellect-intensive. While Model Driven Engineering (MDE) approaches have been popular in downstream Software Development Lifecycle (SDLC) phases to transform the design models into code, there has been a gap in leveraging model-based techniques in document-centric phases of proposal development, requirements analysis, etc. This paper presents an automated proposal development approach for a client-supplied RFP/RFI using a combination of model-based and AI-enabled techniques and describes the case study of its successful deployment to hundreds of presales users across multiple geographies. We explain the Proposal system and report on the experience of deploying the system in the industry, bring out its efficacy, and user feedback, and discuss the lessons learnt. [ABSTRACT FROM AUTHOR]

The Reversed Field Pinch (RFP) configuration looks to be an attractive option for fusion-fission hybrid reactors: the toroidal magnetic systems would be made of copper coils instead of more expensive superconductive magnets; fusion conditions could be reached by ohmic heating only, therefore additional heating systems would not be required; the fission blanket could be located in the most external part of the torus thus facilitating maintenance operations. The paper aims at assessing the potentialities, such as fuel fertilization and/or nuclear waste transmutation and electricity production of a hybrid reactor with a RFP fusion core (R = 6 m, a = 1) whose conceptual design and plasma performances are based on RFX-mod, the largest RFP experiment currently in operation. Fusion conditions can be reached by heating a D-T plasma up to 9.6 keV by ohmic heating, generated by a 20 MA plasma current induced and sustained by flux swing only. The neutron flux (2.1 × 1013 fast neutron/cm2/s) is used to breed tritium in both the inner and outer blanket sections and induce fission reactions in dedicated areas in the external blanket section where Pu + MA (60%)-Zr (40%) rods are located. Both neutronic and safety analyses corroborate the viability of a FFH reactor with a RFP core. [ABSTRACT FROM AUTHOR]

A major modification of the RFX-mod toroidal load assembly has been decided in order to improve passive MHD control and to minimize the braking torque on the plasma, thus extending the operational space in both RFP and Tokamak configurations. With the removal of the vacuum vessel, the support structure will be modified in order to obtain a new vacuum-tight chamber and the first wall tiles will be directly in front of the passive stabilizing shell inside of it, so increasing both the poloidal cross section and the plasma-shell proximity. This implies the design of a new vacuum fit electromagnetic measurement system. The new local probes will be installed in vacuum onto the copper shell, behind the graphite tiles, and shall operate up to a maximum temperature of 180 °C to allow for baking cycles for first wall conditioning. Because of the reduced room available, tri-axial pickup probes have been designed, with the additional advantage of allowing the minimization of alignment errors. The paper describes the detailed design of the new probe set, in particular highlighting advantages and effectiveness of different probe solutions. Preliminary tests carried out on local probe prototypes to characterize their electromagnetic behaviour are also reported. [ABSTRACT FROM AUTHOR]

A major refurbishment of the toroidal complex of the RFX-mod device is in progress and will include the removal of the Inconel vacuum vessel and a modification of the stainless steel supporting structure to be made vacuum tight. The plasma facing graphite tiles will be mounted onto the inner surface of the copper shell so as to increase the plasma proximity factor. New operation regimes are expected to provide a significant reduction of the amplitude of RFP tearing modes with magnetic chaos mitigation and confinement improvement. On the other hand, due to the shorter distance from the passive structures, the plasma is expected to be even more sensitive to magnetic field errors at the plasma boundary, produced by the induced currents near the cuts of the same structures. In preparation to the new RFX-mod2 experiments, a thorough revision of the conditions triggering the error fields due to the eddy currents was undertaken. An analysis of the static and dynamic equilibrium magnetic field has been carried out in RFX-mod and RFX-mod2 to estimate the magnetic field driving the shell eddy currents. A lower equilibrium magnetic field should be required in RFX-mod2. Analyses of different configurations of the poloidal gap were also carried out by a specialized computational tool. A solution with a spaced poloidal gap and a more extended overlapping was envisaged capable of maintaining the same error fields at the closer plasma boundary with the same forcing field and meeting the more stringent insulation and assembly requirements of RFX-mod2. [ABSTRACT FROM AUTHOR]

We developed a novel assay system to quantitatively detect amber codon suppression by tRNAs expressed in plant cells. The assay was based on recovery of the expression of the green fluorescent protein (GFP) as a reporter, in which a fourth Lys codon (AAG) was changed to a premature amber codon TAG, designated as GFP/amber. Plasmids carrying GFP/amber, suppressor tRNA, and red fluorescent protein (RFF) as an internal control, respectively, were introduced into onion epidermal cells to monitor cell numbers with GFP and RFP fluorescence. First, an amber suppressor tRNASer from tobacco (NtS2) to suppress a TAG codon in GFP mRNA was examined, leading to the recovery of GFP fluorescence. Second, we used two different tRNAs (i.e. , AtY3II-am and AtY3II-amiG7), both of which are intron-containing amber suppressor tRNAsTyr, the former impaired precursor-tRNA splicing but the latter did not, as confirmed previously using two different approaches (Szeykowska-Kulinska and Beier, 1991; Akama and Beier, 2003). As expected, coexpression of GFP/amber with AtY3II-am gave no green fluorescence, but significant fluorescence was observed with AtY3II-amiG7. Then, we applied this system for the analysis of 5′-regulatory sequences of the tRNAGln gene family from Arabidopsis. A 5′-flanking sequence of each of the 17 tRNAGln genes was fused to a coding region of an amber suppressor tRNASer gene (NtS2/amber) and its 3′-flanking sequence. Chimeric tRNASer gene, GFP/amber, and RFP were coexpressed, and the GFP or RFP fluorescence intensity was determined in cells using laser-scanning microscopy. In parallel, 17 kinds of original Arabidopsis tRNAGln genes and their chimeric genes with NtS2/amber were all analyzed in cell-free nuclear extract (Yukawa et al., 1997). Comparison of in vitro and in vivo expression of these chimeric tRNA genes displayed generally similar results, accompanied by a wide range of variance in the expression of each gene. Nevertheless, the expression patterns of several genes were clearly the opposite of each other comparing between the two different system, demonstrating the importance of in vivo systems in the study on tRNA expression in plants. • Development of a novel assay system for tRNA expressed in plant cells • The assay based on recovery of the expression of GFP as a reporter • Monitoring the fluorescence of RFP for the normalization of transformation efficiency • Rapid laser-scanning microscopy of GFP or RFP fluorescence for quantitative determination • Its application to determine the expression of 17 kinds of tRNAGln genes from Arabidopsis [ABSTRACT FROM AUTHOR]

The great diversity of wood anatomical features found in trees worldwide results in a broad variety of growth-ring boundary types that are not always easy to recognize, especially in tropical woods. However, the presence of clearly visible limits between tree rings is essential for any tree-ring studies. Here, we propose the use of autofluorescence of wood in order to enhance tree-ring visualization. The multispectral light emitted from the fluorescence stereomicroscope can be filtered in specific wavelengths to improve the visualization of wood anatomical features. To evaluate the effectiveness of this technique, we compared visualization under natural light, GFP (green fluorescent protein) filter, RFP (red fluorescent protein) filter and UV filter. We tested this technique with a set of 38 tree species with different types of growth-ring boundaries. Although results are species-specific, fluorescence has been shown to improve the visualization of growth-ring boundaries by enhancing the contrast among cell types. It may highlight fibrous zones (e.g. Cavanillesia arborea , Aspidosperma polyneuron), different porosity patterns (e.g. Myracrodruon urundeuva), secretory canals (e.g. Copaifera langsdorffii), and parenchyma bands (e.g. Tipuana tipu). Fluorescence allows the visualization of growth-ring boundaries in species that were previously described as having indistinct growth rings under natural light. For species with clear tree-ring boundaries such as Cedrela fissilis and Hymenaea courbaril , this approach aids the identification of false rings. In addition to the visualization of growth-ring boundaries, autofluorescence may be useful for other qualitative and quantitative analyses of wood anatomy, such as wood identification and automated measurements of anatomical features. Scientists struggling with tree-ring counting and cross-dating due to difficult tree-ring visualization may find fluorescence useful. It may also aid to identify new species suitable for tree-ring studies. [ABSTRACT FROM AUTHOR]

Newcastle disease virus (NDV) has been used as a vector in the development of vaccines and gene therapy. A majority of these NDV vectors express only a single foreign gene through either an independent transcription unit (ITU) or an internal ribosomal entry site (IRES). In the present study, we combined the ITU and IRES methods to generate a novel NDV LaSota strain-based recombinant virus vectoring the red fluorescence protein (RFP) and the green fluorescence protein (GFP) genes. Biological assessments of the recombinant virus, rLS/IRES-RFP/GFP, showed that it was slightly attenuated in vivo , yet maintained similar growth dynamics and viral yields in vitro when compared to the parental LaSota virus. Expression of both the RFP and GFP was detected from the rLS/IRES-RFP/GFP virus-infected DF-1 cells by fluorescence microscopy. These data suggest that the rLS/IRES-RFP/GFP virus may be used as a multivalent vector for the development of vaccines and gene therapy agents. [ABSTRACT FROM AUTHOR]

A major refurbishment of the toroidal load assembly is under study to extend the operational space of RFX-mod in both RFP and Tokamak configurations. It includes the removal of the vacuum vessel to increase the plasma-shell proximity and modifications of the support structure to obtain a new vacuum-tight chamber. This entails the design of a new electromagnetic measurement system, taking into account the requirements of both the equilibrium and MHD control system and physics analyses requirements in both RFP and shaped Tokamak configurations. The spatial resolution is constrained by the number of graphite tiles, 28 (poloidal) x 72 (toroidal), which cover the inner surface of the toroidal vacuum chamber. Alternative layouts of 4 x 72 and 7 x 72 are being considered to assure the calculation of the MHD mode spectrum up to (m = 0,1,2 or 3; n = 0,…,±35). A substantial advantage in terms of harmonic spectrum reconstruction is expected also in case of sensor faults with respect to the present 4x48 arrangement. Moreover, 12 poloidal arrays will include 14 sensors to compute the harmonic content needed by the plasma boundary reconstruction algorithm (m = 0,…,6). Due to the reduced room between the copper shell and the graphite tiles, the design of triaxial, vacuum fit pickup probes have started also for their convenience in compensating alignment errors. [ABSTRACT FROM AUTHOR]

The mechanism of discontinuous transcription for the synthesis of a series of sub-genomic mRNAs to express the structural proteins of porcine reproductive and respiratory syndrome virus (PRRSV) potentially allows for the simultaneous expression of multiple foreign genes. This can occur by insertion of multiple novel independent transcription units between the ORF sequences of the PRRSV genome. Here, an expression cassette consisting of a red fluorescent protein (RFP) gene flanked at its 3′ end by transcription-regulating sequences (TRS) and an expression cassette consisting of an iLOV gene flanked at its 5′ end by TRS, was constructed. The resulting expression cassette containing a RFP and an iLOV gene were introduced between ORF1b and 2 as well as ORF7 and 3′UTR, respectively, in an infectious PRRSV cDNA clone. Transfection of the resulting clone (pGX-12RFP-73iLOV) into cells resulted in the recovery of a recombinant virus (rGX-12RFP-73iLOV). Simultaneous expression of RFP and iLOV was observed in MARC-145 cells infected with rGX-RFP-iLOV. To test the ability of the PRRSV genome to express all three reporter genes simultaneously, an expression cassette containing the Gluc gene and one containing the iLOV gene were also inserted in between ORF1b and 2 as well as ORF7 and 3′UTR, respectively. This was performed in a recently obtained infectious PRRSV cDNA clone carrying a RFP gene in nsp2. Transfection of the construct (pGX-R-Gluc-iLOV) carrying the three reporter genes into cells allowed the rescue of the recombinant reporter virus (rGX-R-Gluc-iLOV) which showed similar growth characteristics to the parental virus but yielded 100-fold less infectious viruses. Fluorescence microscopy of cells infected with rGX-R-Gluc-iLOV demonstrated the presence of both RFP and iLOV genes. Gluc activities in supernatants harvested at different time points from cells infected with recombinant viruses carrying Gluc showed increased levels of Gluc activity as the infection progressed. This indicated that Gluc gene as well as its activity were acceptable parameters to monitor viral propagation. Our results indicate that it is possible to introduce at least three foreign proteins simultaneously in a PRRSV-based vector and such studies will prove invaluable in our future understanding of these viruses. • Construction and characterization of a PRRSV expressing iLOV in an expression cassette inserted between ORF7 and 3′UTR. • Construction and recovery of full-length PRRSV cDNA clones expressing two reporter proteins. • Construction and recovery of a full-length PRRSV cDNA clone expressing three reporter proteins. [ABSTRACT FROM AUTHOR]

A data acquisition and service system based on HDF5 has been designed. It includes four components: data acquisition console, data acquisition subsystem, data archive system and data service. The data acquisition console manages all DAQ information and controls the acquisition process. The data acquisition subsystem supports continuous data acquisition with different sampling rates which can be divided into low, medium and high level. All experimental data will be remotely transferred to the data archive system. It adopts HDF5 as its low-level data storage format. The hierarchical data structure of HDF5 is useful for efficiently managing the experimental data and allows users to define special data types and compression filter which can be useful to deal with special signals. Several data service tools have also been developed so that users can get data service via Client/Server or Brower/Server. The system will be demonstrated on Keda Torus eXperiment (KTX) device, which is a new Reversed Field Pinch (RFP) magnetic confinement device. The details are presented in the paper. [ABSTRACT FROM AUTHOR]

The conductive shell in reversed field pinch devices plays an important role in controlling plasma and in suppressing MHD instabilities. The shell in the Keda torus experiment reversed field pinch (KTX-RFP) device includes a 6-mm stainless steel vacuum chamber and a 1.5-mm stabilizing copper shell. This stabilizing shell has both poloidal and toroidal gaps to allow a coupling of electromagnetic energy to the plasma. Nevertheless, any gaps in this shell generate error fields. A 3D electromagnetic field model has been used to study effects of the gap on the shell. Using off-center current filaments instead of the distributed plasma current density, numerical analyses render the induced current distribution on the stabilizing shell and the resultant error field distribution at a specific frequency. From the analyses and comparisons of different configurations for the stabilizing shell, a suitable shell design is chosen consisting of three sections: one primary shell, two poloidal shield shells, and two toroidal shield shells. Moreover, the time evolution of the magnetic field inside and outside the stabilizing shell was obtained for this design and the dependence of the magnetic field penetration time on mode number and location has been investigated. [ABSTRACT FROM AUTHOR]

In magnetic confinement fusion devices, close-fitting structures surrounding the plasma are needed to slow down the growth rate of MHD unstable modes to time scales accessible to real time feedback control systems. However, error fields are generated as the response of the passive structures to the dynamic equilibrium field configuration, due to the presence of the cuts needed to allow the penetration of electric and magnetic fields. Considerable improvements have been achieved in RFX-mod after starting operation of the active MHD control system, allowing the successful simultaneous feedback control of a wide spectrum of MHD instabilities. In this paper, a feasibility study of a new local active correction system of magnetic field errors is presented, together with a comparison of three different configurations of the passive structure cuts. [ABSTRACT FROM AUTHOR]

Abstract: Exosomes play an important role in cell-to-cell communication to promote tumor metastasis. In order to image the fate of cancer-cell-derived exosomes in orthotopic nude mouse models of breast cancer, we used green fluorescent protein (GFP)-tagged CD63, which is a general marker of exosomes. Breast cancer cells transferred their own exosomes to other cancer cells and normal lung tissue cells in culture. In orthotopic nude-mouse models, breast cancer cells secreted exosomes into the tumor microenvironment. Tumor-derived exosomes were incorporated into tumor-associated cells as well as circulating in the blood of mice with breast cancer metastases. These results suggest that tumor-derived exosomes may contribute to forming a niche to promote tumor growth and metastasis. Our results demonstrate the usefulness of GFP imaging to investigate the role of exosomes in cancer metastasis. [Copyright &y& Elsevier]

Abstract: A closed-loop control system of the reversal parameter has been implemented in the RFX-mod machine on the basis of a model of the toroidal field system including the toroidal power supply circuit, with a description of the coupling with the plasma current and the local control of the power section. In the paper, after a description of the power supply model and the implemented closed-loop control, the outcome of the work will be reported by showing some experimental results on RFX-mod high current dedicated campaigns, with the feedback control system in operation. [Copyright &y& Elsevier]

Abstract: The paper presents an overview on the power supply (PS) systems for plasma instabilities control in fusion experiments, based on active control coils. First, the MHD instabilities and the approach to their control in Tokamaks and Reversed Field Pinches (RFPs) are described. Then, the features of MHD modes controls presently used in fusion experiments are reviewed. For the control systems based on active coils fed by fast power supplies, the typical requirements in terms of power, dynamics, accuracy and delay are summarized and discussed. Then, a survey on the technology available to design these types of PSs is given, together with the most suitable circuit topologies and guidelines for the design, on the basis of solutions adopted in existing experiments. [Copyright &y& Elsevier]

Abstract: The double-cassette expression vector strategy is valuable for many studies, including comparative analysis of the function of promoters and expression of genes in different compartments. In this study, we report co-expression of enhanced yellow fluorescent protein (EYFP) and red fluorescent protein (RFP) in Eimeria tenella transfected with two double-cassette expression vectors, pMIC-EYFP/ACT-RFP and pMIC-EYFP/ACTss-RFP. The results showed that under regulation of the mic1 promoter, EYFP was expressed in sporulated oocysts but not in unsporulated ones, while under regulation of the actin promoter RFP was expressed in both forms. We found that the signal peptide of Toxoplasma gondii dense granule protein 8 (GRA8) located the RFP expression to the parasitophorous vacuoles of the parasites, the margins of the unsporulated oocysts and the cavities of the sporocysts. The feasibility of co-expression of exogenous proteins in E. tenella is important for the development of transgenic E. tenella as a novel vaccine vector. [Copyright &y& Elsevier]

Abstract: A synthetic regulatory construct based on a two-stage amplifying promoter cascade is applied to whole-cell biosensing. Green fluorescent protein (GFP) and red fluorescent protein (RFP) enable two-component tracking of the response event, enabling the system to exhibit increased sensitivity, a lower limit of detection, and a unique optical density-free assessment mode. Specifically, the recA and tac promoters are linked by the LacI repressor in Escherichia coli, where DNA-damage activates the recA promoter and the up-regulation of GFP and LacI proteins. LacI represses the tac promoter, down-regulating the otherwise constitutive mCherry transcription. The response of the construct was compared with two singly tagged, conventional recA promoter-reporter constructs: recA::gfpmut3.1 and recA::mCherry. Using a miniature LED-based flow-through optical detector developed for remote sensing applications, limits of detection for the dual reporter construct reached as low as 0.1nM MMC. By comparison, single-ended reporters recA::mCherry and recA::gfpmut3.1 achieved best limits of detection of 0.25nM and 2.0nM, respectively. An approach to three-component optical analysis, based on a system of detectors with coupled calibration equations enables accurate assessments of the red fluorescence, green fluorescence, and biomass of sensor cell suspensions. The system approach is effective at overcoming interferences caused by optically dense samples and overlapping fluorescence spectra. Such a technique may be useful in studying the biological mechanisms which underlie the synthetic regulatory device of this work and others. [Copyright &y& Elsevier]

Abstract: RET finger protein (RFP), which belongs to the large B-box RING finger protein family, has been reported to be expressed in breast carcinoma cell lines. We conducted this study to evaluate the expression level of RFP in breast carcinomas. Tissue microarrays were constructed from 133 cases of invasive breast carcinoma. Sections obtained from tissue arrays and whole sections from 10 non-neoplastic breast tissues were stained for ER, PR, ErbB2, and RFP using immunohistochemistry, and ErbB2 gene status was evaluated by FISH. The correlation between various immunohistochemical markers was determined. We found nuclear RFP expression in 41.4% of invasive carcinomas and in none of the non-neoplastic breast tissues. We also stained whole sections of 29 cases of invasive breast carcinoma, which included the TMA study, and 10 cases of ductal carcinoma in situ (DCIS) by RFP. We observed that four (40%) of the DCIS cases were positive with RFP. In statistical analysis, there was a significant, positive association between RFP and ErbB2 status at the protein level (p=0.002) and the gene level (p=0.049). There was no statistically significant association between the expression of RFP and tumor size, LN status, ER, PR, and grade (p>0.05). However, we found a significant association between age and RFP expression. RFP expression was stronger in patients 50 years or older (p=0.048). In our study, the expression of RFP correlated strongly with ErbB2 status. Our study is the first in the literature to show expression of RFP in patients with breast carcinoma. However, the possible role of RFP in breast carcinogenesis needs to be investigated in more detailed clinical and experimental studies. [Copyright &y& Elsevier]

Abstract: Monodisperse poly(lactide-co-glycolide) (PLGA) microspheres containing rifampicin (RFP), anti-tubercle drug, as hydrophobic model drug were prepared by solvent evaporation method with a membrane emulsification technique using Shirasu Porous Glass (SPG) membranes. Five kinds of rifampicin-loaded PLGA (RFP/PLGA) microspheres with different sizes were prepared by changing pore size of the membranes. Effect of polyethylene glycol (PEG) added to polyvinyl alcohol (PVA) solution (continuous phase) upon the monodispersity of microspheres was studied. PEG was used as a stabilizer for microspheres dispersing in PVA solution. The most suitable molecular weight of PEG as a stabilizer was 20,000. RFP/PLGA microspheres prepared with PEG20000 were apparently more uniform than those prepared without PEG. The yield of RFP/PLGA microspheres was 100%. The initial burst observed in the release of RFP from RFP/PLGA microspheres was suppressed by the addition of PEG. [Copyright &y& Elsevier]

Abstract: Revealing the connections of neuronal systems is critical for understanding how they function. The vast majority of neurons in all cortical areas consist of excitatory cells whose activity is controlled by inhibitory cells. Distribution and projection patterns of inhibitory and excitatory cells are key information to understand the organization of the nervous system. To investigate axonal projections, we developed a method to uniquely distinguish excitatory axons from inhibitory ones in the cortex using transgenic mice expressing Cre recombinase in the Ca2+/calmodulin-dependent protein kinase IIα-containing neurons. These animals were injected by an adenoviral vector engineered so that it directs red fluorescent protein expression in non-Cre-expressing cells, and green fluorescent protein in Cre-positive neurons. We demonstrated in vitro and in vivo that GFP-expressing neurons are GABA-immunonegative (excitatory), while the RFP-expressing cells are either GABAergic neurons or glial cells. One week after the viral vector injection RFP and GFP signals overlapped in a subset of cells but after 1 month, the two signals showed total segregation. Six months post-inoculation, GFP-labelling was clearly visible in axons but RFP remained only in somata and proximal dendrites. This technique can thus be used to differentiate excitatory axonal projections from inhibitory ones, and represent a unique tool in neuronal circuit analysis. [Copyright &y& Elsevier]

Abstract: The recent modifications brought to the RFX experiment, now RFX-mod, pose a strong focus on the active control of the plasma magnetic configuration. The new machine layout features a thin stabilizing shell which makes active control feasible and necessary at the same time. The new set of actuators includes a complex saddle coils system and a redesigned power supply system driving the toroidal field coils. The new toroidal power supply system allows a great flexibility since each sector, composed by four coils, is driven by an independent d.c./d.c. switching converter, in series with the main power supply providing the bias current. The current control on the load, performed by the four quadrant fast switching converter, allows producing the desired waveforms of toroidal field. To fully exploit the capabilities of such a system two different controllers have been designed enabling to track automatically a selected evolution of either the toroidal field at the plasma edge or the reversal F parameter, respectively.The new control systems allow to perform experiments in specified plasma configurations targeting particular physics issues and improved the overall reproducibility of the RFX-mod discharges. [Copyright &y& Elsevier]

Abstract: Aiming at predictive simulation of the plasma magnetic parameters during the RFX-mod discharges, a model was designed to simulate the poloidal field power supply system, detailing each individual switching unit and the single ac–dc converter with its local control circuit, together with the magnet system and the complete multi-variable feedback system devoted to the plasma shape and position control. The model was realized by coupling a linear model of the axi-symmetric evolution of the plasma with a detailed implementation of the RFX-mod circuit developed by means of the SimPowerSystem Toolbox in the framework of the Matlab-Simulink™ package. In order to evolve the plasma current, a Bessel Function Model (BFM) is adopted to implement the energy transfer from the toroidal to the poloidal field. The axi-symmetric controller is implemented to close the loop and is the same running on the VME nodes of the real control system. The model was successfully tested against experimental data, showing good agreement between measured and calculated waveforms. [Copyright &y& Elsevier]

Abstract: In the Reversed Field Pinch eXperiment RFX-mod a Gas Puffing Imaging Diagnostic (GPID) is used to investigate the dynamical structure of plasma edge turbulence. The GPID measures the radiation emitted from He gas puffed in H discharges and allows the investigation of edge plasma properties with high time and space resolution at high plasma current during the entire discharge. The characterization of edge turbulence has been carried out in terms of power spectrum, cross-correlation and toroidal velocity of structures. The scaling of such velocity with the density corresponds to different values of the dimensionless ratio ν ci/ν ei (ion cyclotron frequency/electron–ion collision frequency) ranging from below to above unity. The toroidal characteristic length of intermittent structures at different time scales has been determined by applying the conditional average technique on the structures identified by the continuous wavelet transform. These lengths are in the range 15–45mm, while the number of structures increases with the electron density, the propagation velocity, opposite to the plasma current, decreases with the density and saturates at −20km/s. [Copyright &y& Elsevier]

Abstract: Inorganic arsenic has strong human carcinogenic potential, but the availability of an animal model to study toxicity is extremely limited. Here, we used the transgenic zebrafish line Tg(k18(2.9):RFP) as an animal model to study arsenite toxicity. This line was chosen because the red fluorescent protein (RFP) is expressed in stratified epithelia (including skin), due to the RFP reporter driven by the promoter of the zebrafish keratin 18 gene. We titrated doses of inorganic arsenite for zebrafish embryos and found that arsenite exposure at 50μM for 120h was suitable for mimicking a long-term, chronic effect. When embryos derived from Tg(k18(2.9):RFP) adults were treated with this arsenite dose and time of exposure, abnormal phenotypes were not noticeable under the light microscope. However, arsenic keratosis was visible in the epithelial cells under the fluorescent microscope. Morphological defects became more severe with increased dose and exposure duration, suggesting that the severity of skin lesions was dose- and time-dependent. Histochemical examination of keratosis after 4′,6′-diamidino-2-phenylindole hydrochloride (DAPI) staining showed that the epithelial cells overproliferated after treatment with arsenite. Therefore, this Tg(k18(2.9):RFP) zebrafish line is an excellent model for studying toxicity induced by inorganic arsenite and may have potential for studying other environmental pollutants. [Copyright &y& Elsevier]

Abstract: Human CstF-77 is one of the three subunits of cleavage stimulation factor (CstF) that is essential for mRNA polyadenylation. Its Drosophila homologue, suppressor of forked [su(f)], contains an intronic poly(A) site, which can lead to a short transcript without a stop codon. By both bioinformatic searches and validation with molecular biology experiments, we found that human and mouse CstF-77 genes also contain an intronic poly(A) site, which can be utilized to produce short CstF-77 transcripts lacking sequences encoding domains that are involved in many of the CstF-77 functions. The genomic sequence surrounding the poly(A) site is highly conserved among all vertebrates, but is not present in non-vertebrate species. Using public Serial Analysis of Gene Expression (SAGE) data, we found that the intronic poly(A) site is utilized in a wide range of tissues. This finding indicates that vertebrates may employ a similar alternative polyadenylation mechanism to modulate CstF-77, highlighting the importance of the regulation of CstF-77 in various species. [Copyright &y& Elsevier]

We propose a new cell biology where the behavior of cells can be visualized in the living animal. An example of the new cell biology is dual-color fluorescence imaging using red fluorescent protein (RFP)-expressing tumors transplanted in green fluorescent protein (GFP)-expressing transgenic mice. These models show with great clarity the details of tumor–stroma interactions and especially tumor-induced angiogenesis, tumor-infiltrating lymphocytes, stromal fibroblasts and macrophages. Another example is the color coding of cells with RFP or GFP such that both cell types can be simultaneously visualized in vivo. Stem cells can also be visualized and tracked in vivo. Mice in which the regulatory elements of the stem-cell marker nestin drive GFP enable nascent vasculature to be visualized interacting with transplanted RFP-expressing cancer cells. Nestin-driven GFP expression can also be used to visualize hair follicle stem cells. Dual-color cells expressing GFP in the nucleus and RFP in the cytoplasm enable real-time visualization of nuclear-cytoplasm dynamics including cell cycle events and apoptosis. Multiple-color labeling of cells will enable multiple events to be simultaneously visualized in vivo including gene expression, ion fluxes, protein and organelle trafficking, chromosome dynamics and numerous other processes currently still only studied in vitro. [Copyright &y& Elsevier]

The new RFX load assembly is characterized by a thin copper shell with a 50 ms time constant, for the vertical field penetration, and 192 active saddle coils all around the torus. The active control of resistive wall modes (RWM''s) is considered a basic issue to extend the pulse duration to its nominal value. An open loop state space model was developed in view of the simultaneous control of different harmonic components of the magnetic field. It integrates a lumped parameter electromagnetic model of the saddle coil system and a linear model of the plasma magneto-hydro-dynamic (MHD) evolution. Reduction techniques have already been tested in order to obtain a model suitable for the design of the control system of the plasma modes. [Copyright &y& Elsevier]

: ObjectivesWe sought to investigate whether pseudonormal (PN) filling was associated with death or hospital admission in patients with congestive heart failure (CHF).: BackgroundThe high mortality rate associated with CHF is related to many clinical and echocardiographic variables. In particular, a short mitral deceleration time and restrictive diastolic filling predict death and/or hospital admission. We hypothesized that differentiating patients with nonrestrictive filling might identify an intermediate PN group that may be associated with intermediate risk.: MethodsA total of 115 patients admitted to the hospital for exacerbation of CHF symptoms underwent pre-discharge Doppler echocardiography to determine mitral inflow (before and after preload reduction) and pulmonary venous return. Patients were followed up for one year, and all-cause mortality and re-admission data were analyzed.: ResultsThe classification of filling patterns was: abnormal relaxation (AR) in 46 (40%) patients, pseudonormal (PN) filling in 42 (36.5%) patients and restrictive filling pattern (RFP) in 27 (23.4%) patients. When comparing the RFP group with the AR group, all-cause mortality was higher (38.4% vs. 17.4%, p = 0.033), hospital admission was higher (70.3% vs. 54.3%, p = 0.073), death/hospital admission was higher (77.8% vs. 56.5%, p = 0.02), CHF hospital admission was higher (40.7% vs. 15.2%, p = 0.01) and death/CHF hospital admission was higher (62.9% vs. 26.1%, p = 0.0005). Mortality in the PN group was not significantly different from that in the two other groups, but re-admissions were higher than the AR group (76.2% vs. 54.3%, p = 0.006), as was death/re-admission (78.6% vs. 56.5%, p = 0.004) and death/CHF re-admission (47.6% vs. 26.1%, p = 0.03). Re-admissions in the PN and RFP groups were comparable.: ConclusionsIn a general hospital population of older patients with CHF, PN filling was associated with hospital admission rates similar to those seen with restrictive filling. The combined end point of death/CHF hospital admission was similar for restrictive filling and AR. Measurement of these variables is easy to add to routine clinical echocardiography and may provide important prognostic information in a wide range of patients with CHF. [Copyright &y& Elsevier]