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[Display omitted] • Oleaginous yeast Apiotrichum brassicae is a good producer of food lipid alternatives. • The fatty acid and TAG composition of A. brassicae lipids resembles cocoa butter. • The crystallisation and melting behaviour and solid fat content, were also analysed. • A pilot-scale process can make lipids similar to cocoa butter, palm oil or beef fat. Oleaginous yeasts offer a promising sustainable alternative for producing edible lipids, potentially replacing animal and unsustainable plant fats and oils. In this study, we screened 11 oleaginous yeast species for their lipid profiles and identified Apiotrichum brassicae as the most promising candidate due to its versatility across different growth media. A. brassicae grown in a dairy side stream produced lipids with a composition most similar to cocoa butter, but the stearic acid and linoleic acid content varied greatly when grown on different substrates. We visualised the formation of lipid droplets by digital holotomography. Pilot-scale production was followed by enzymatic and ultrasonic treatment of biomass and heptane/ethanol extraction. The fatty acid (FA) and triacylglycerol (TAG) composition, thermal behaviour, and solid fat content of A. brassicae lipids was compared to benchmarks such as beef fat, cocoa butter, palm oil and milk fat. The FA profile of the A. brassicae lipids shares the same types of fatty acids with cocoa butter, beef fat and palm oil, however concentrations differ resulting in a lower content of saturated FAs. This increased the proportion of unsaturated TAGs, reducing the melting and crystallisation temperatures and the solid fat content. The microbial lipids contained the major TAGs of cocoa butter at similar ratios, resulting in a comparable melting peak and crystallisation peaks similar to the low-melting groups of beef fat and palm oil. Fractionation has the potential to produce beef fat, cocoa butter or palm oil equivalents with desired techno-functional properties. This study demonstrates the potential of A. brassicae to produce tailored lipid profiles for various food applications through strain and process engineering or downstream processing. [ABSTRACT FROM AUTHOR]

[Display omitted] • Whey protein cryogel particles were obtained via hydrogel grinding and freeze-drying. • Hydrogel pH affects cryogel particle density, porosity and dimension. • Cryogel particles produced at the isoelectric pH (pI) form a network in oil. • Spreadable oleogels are obtained from cryogel particles produced at the pI. • Cryogel particles produced at the pI can be used in low-saturated-fat cocoa spreads. Cryogel particles were obtained by freeze-drying and grinding hydrogel monoliths made from 20 % (w/w) whey protein isolate (WP) suspensions prepared at different pH (pH 4.8, 5.7, and 7.0). The microstructure, porosity, and density of the cryogels were strongly affected by the starting pH of the suspension. At pH 4.8, corresponding to the isoelectric point, proteins assumed a globular form leading to a cryogel with the highest porosity and lowest density compared to those formed at higher pH values (5.7 and 7.0). Such morphological differences accounted for different oil structuring capabilities. When mixed with oil, the cryogel particles formed at the pI were capable of entrapping larger quantities of oil (∼63 % w/w) than those obtained distant from the pI (∼47 %, w/w), forming a spreadable material. In this system, as confirmed by confocal microscopy, WP particles were evenly distributed in oil forming a network connected by capillary bridges and surface hydrophilic interactions. Thus, the mixture of sunflower oil with cryogel particles formed at the pI allowed to obtain an oleogel, exploitable for fat replacement, as confirmed by the preparation of a cocoa spread prototype. Results highlighted the critical impact of protein hydrogel structure in determining the ability of the cryogel particles thereof to entrap oil and tune the oleogel characteristics. The potentialities of this innovative material as ingredient of low saturated fat food products were also demonstrated. [ABSTRACT FROM AUTHOR]