10 results on '"Ruppert, Clemens"'
Search Results
2. Impact of surface tension on the conversion rate of large to small surfactant aggregates
- Author
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Ruppert, Clemens, Pucker, Carolin, Markart, Philipp, Seibold, Kerstin, Bagheri, Ariane, Grimminger, Fritz, Seeger, Werner, and Günther, Andreas
- Published
- 2003
- Full Text
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3. Prevention of Lung Fibrosis in a Rabbit Model of Bleomycin-Induced Lung Fibrosis Upon Aerosol Application of Heparin or Urokinase(*)
- Author
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Gunther, Andreas, Lubke, Norbert, Ruppert, Clemens, Weissman, Norbert, Grimminger, Friedrich, and Seeger, Werner
- Subjects
Pulmonary fibrosis -- Research ,Health ,Research - Abstract
(CHEST 2001; 120:4S-5S) Persistent alveolar fibrin formation may promote the development of lung fibrosis. To investigate this issue, pulmonary fibrosis was induced in New Zealand rabbits by aerosol application of [...]
- Published
- 2001
4. Biophysical inhibition of pulmonary surfactant function by polymeric nanoparticles: Role of surfactant protein B and C.
- Author
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Beck-Broichsitter, Moritz, Ruppert, Clemens, Schmehl, Thomas, Günther, Andreas, and Seeger, Werner
- Subjects
PULMONARY surfactant ,NANOPARTICLES ,PULMONARY surfactant-associated protein B ,BIOPHYSICS ,PHOSPHOLIPIDS - Abstract
The current study investigated the mechanisms involved in the process of biophysical inhibition of pulmonary surfactant by polymeric nanoparticles (NP). The minimal surface tension of diverse synthetic surfactants was monitored in the presence of bare and surface-decorated (i.e. poloxamer 407) sub-100 nm poly(lactide) NP. Moreover, the influence of NP on surfactant composition (i.e. surfactant protein (SP) content) was studied. Dose-elevations of SP advanced the biophysical activity of the tested surfactant preparation. Surfactant-associated protein C supplemented phospholipid mixtures (PLM-C) were shown to be more susceptible to biophysical inactivation by bare NP than phospholipid mixture supplemented with surfactant protein B (PLM-B) and PLM-B/C. Surfactant function was hindered owing to a drastic depletion of the SP content upon contact with bare NP. By contrast, surface-modified NP were capable of circumventing unwanted surfactant inhibition. Surfactant constitution influences the extent of biophysical inhibition by polymeric NP. Steric shielding of the NP surface minimizes unwanted NP–surfactant interactions, which represents an option for the development of surfactant-compatible nanomedicines. [ABSTRACT FROM AUTHOR]
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- 2014
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- View/download PDF
5. Biophysical investigation of pulmonary surfactant surface properties upon contact with polymeric nanoparticles in vitro.
- Author
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Beck-Broichsitter, Moritz, Ruppert, Clemens, Schmehl, Thomas, Guenther, Andreas, Betz, Thomas, Bakowsky, Udo, Seeger, Werner, Kissel, Thomas, and Gessler, Tobias
- Subjects
BIOPHYSICS ,PULMONARY surfactant ,NANOPARTICLES ,DRUG carriers ,RESPIRATORY therapy ,STYRENE ,DRUG delivery systems ,SURFACE tension - Abstract
Abstract: Nanoparticulate drug carriers have been proposed for the targeted and controlled release of pharmaceuticals to the lung. However, inhaled particles may adversely affect the biophysical properties of pulmonary surfactant. This study examines the influence of polymeric nanoparticles with distinct physicochemical properties on the adsorption and dynamic surface tension lowering properties of pulmonary surfactant. Nanoparticles had a mean size of 100 nm with narrow size distributions. Although poly(styrene) and poly(D,L-lactide-co-glycolide) nanoparticles revealed a dose-dependent influence on biophysics of pulmonary surfactant, positively-charged nanoparticles made from poly(butyl methacrylate-co-(2-dimethylaminoethyl) methacrylate-co-methyl methacrylate) showed no effect. This behavior is attributed to the differences in ζ-potential and surface hydrophobicity, which in turn involves an altered adsorption pattern of the positively charged surfactant proteins to the nanoparticles. This study suggests that polymeric nanoparticles do not substantially affect the biophysical properties of pulmonary surfactant and may be a viable drug-delivery vehicle for the inhalative treatment of respiratory diseases. From the Clinical Editor: Inhaled nanoparticulate drug carriers may adversely affect the biophysical properties of pulmonary surfactant. In this study the influence of polymeric nanoparticles was characterized from this standpoint, with the conclusion that polymeric nanoparticles do not substantially affect the biophysical properties of pulmonary surfactant and may be viable drug-delivery vehicles for inhalational treatment. [Copyright &y& Elsevier]
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- 2011
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6. Cell-Specific Nitric Oxide Synthase-Isoenzyme Expression and Regulation in Response to Endotoxin in Intact Rat Lungs.
- Author
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Ermert, Monika, Ruppert, Clemens, Gunther, Andreas, Duncker, Hans-Rainer, Seeger, Werner, and Ermert, Leander
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- 2002
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- View/download PDF
7. The diverging roles of insulin-like growth factor binding proteins in pulmonary arterial hypertension.
- Author
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Schlueter, Beate Christiane, Quanz, Karin, Baldauf, Julia, Petrovic, Aleksandar, Ruppert, Clemens, Guenther, Andreas, Gall, Henning, Tello, Khodr, Grimminger, Friedrich, Ghofrani, Hossein-Ardeschir, Weissmann, Norbert, Seeger, Werner, Schermuly, Ralph Theo, and Weiss, Astrid
- Subjects
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INSULIN-like growth factor-binding proteins , *PULMONARY arterial hypertension , *SOMATOMEDIN C , *EPIDERMAL growth factor receptors , *PULMONARY hypertension , *ENDOTHELIN receptors , *BONE morphogenetic protein receptors - Abstract
Pulmonary hypertension (PH) is a progressive, severe and to date not curable disease of the pulmonary vasculature. Alterations of the insulin-like growth factor 1 (IGF-1) system are known to play a role in vascular pathologies and IGF-binding proteins (IGFBPs) are important regulators of the bioavailability and function of IGFs. In this study, we show that circulating plasma levels of IGFBP-1, IGFBP-2 and IGFBP-3 are increased in idiopathic pulmonary arterial hypertension (IPAH) patients compared to healthy individuals. These binding proteins inhibit the IGF-1 induced IGF-1 receptor (IGF1R) phosphorylation and exhibit diverging effects on the IGF-1 induced signaling pathways in human pulmonary arterial cells (i.e. healthy as well as IPAH-hPASMCs, and healthy hPAECs). Furthermore, IGFBPs are differentially expressed in an experimental mouse model of PH. In hypoxic mouse lungs, IGFBP-1 mRNA expression is decreased whereas the mRNA for IGFBP-2 is increased. In contrast to IGFBP-1, IGFBP-2 shows vaso-constrictive properties in the murine pulmonary vasculature. Our analyses show that IGFBP-1 and IGFBP-2 exhibit diverging effects on IGF-1 signaling and display a unique IGF1R-independent kinase activation pattern in human pulmonary arterial smooth muscle cells (hPASMCs), which represent a major contributor of PAH pathobiology. Furthermore, we could show that IGFBP-2, in contrast to IGFBP-1, induces epidermal growth factor receptor (EGFR) signaling, Stat-3 activation and expression of Stat-3 target genes. Based on our results, we conclude that the IGFBP family, especially IGFBP-1, IGFBP-2 and IGFBP-3, are deregulated in PAH, that they affect IGF signaling and thereby regulate the cellular phenotype in PH. [Display omitted] [ABSTRACT FROM AUTHOR]
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- 2024
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8. Ischemic and Endotoxin Pre-Conditioning Reduce Lung Reperfusion Injury-Induced Surfactant Alterations
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Markart, Philipp, Schmidt, Reinhold, Ruppert, Clemens, Höres, Christina, Silber, Rolf E., Börgermann, Jochen, Günther, Andreas, and Friedrich, Ivar
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ISCHEMIA , *REPERFUSION injury , *LUNG transplantation , *LUNG diseases , *ENDOTOXINS , *PULMONARY embolism - Abstract
Background: Pulmonary ischemia/reperfusion injury represents a common clinical phenomenon after lung transplantation, pulmonary embolism, and cardiac surgery with extracorporeal circulation. We investigated the influence of ischemic and endotoxin pre-conditioning on gas exchange and surfactant properties in a canine model of ischemia/reperfusion injury. Methods: Twenty-six foxhounds underwent 3 hours of warm ischemia of the left lung, followed by 8 hours of reperfusion. Ischemic pre-conditioning was performed for either 5 minutes (IPC-5) or by 2 10-minute ischemic periods (IPC-10), before ischemia. For endotoxin pre-conditioning, dogs were pre-treated by a daily intravenous application of increasing amounts of endotoxin for 6 days. No pre-conditioning was performed in the controls. Bronchoalveolar lavage was performed before ischemia/reperfusion injury (baseline) and after the 8-hour reperfusion period in the non-injured right and in the reperfused left lung. Bronchoalveolar lavage fluids were analyzed for the phospholipid-protein ratio, the content of large surfactant aggregates, the phospholipid and neutral lipid profile, the surfactant protein (SP) content, and for biophysical activity. Results: Severe surfactant alterations were observed in the ischemia/reperfusion-injured left lung, with increased protein concentrations and depressed concentrations of large surface aggregates, SP-B, dipalmitoylated phosphatidylcholine, and phosphatidylglycerol. Endotoxin pre-conditioning and IPC-5 were both capable of greatly preventing the ischemia/reperfusion injury-related deterioration of surfactant properties. IPC-10 exerted no effects. Endotoxin pre-conditioning and IPC-5, but not IPC-10, also prevented loss of gas exchange. Conclusions: Ischemic and endotoxin pre-conditioning may protect against impairment of gas exchange in ischemia/reperfusion injury by restoring physiological surfactant properties. [Copyright &y& Elsevier]
- Published
- 2005
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9. Loss of LRP1 promotes acquisition of contractile-myofibroblast phenotype and release of active TGF-β1 from ECM stores.
- Author
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Schnieder, Jennifer, Mamazhakypov, Argen, Birnhuber, Anna, Wilhelm, Jochen, Kwapiszewska, Grazyna, Ruppert, Clemens, Markart, Philipp, Wujak, Lukasz, Rubio, Karla, Barreto, Guillermo, Schaefer, Liliana, and Wygrecka, Malgorzata
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MYOFIBROBLASTS , *TRANSFORMING growth factors , *INTEGRINS , *GENE expression profiling , *EXTRACELLULAR matrix , *TRANSGENIC mice , *FIBROBLASTS - Abstract
In healing tissue, fibroblasts differentiate to α-smooth muscle actin (SMA)-expressing contractile-myofibroblasts, which pull the wound edges together ensuring proper tissue repair. Uncontrolled expansion of the myofibroblast population may, however, lead to excessive tissue scarring and finally to organ dysfunction. Here, we demonstrate that the loss of low-density lipoprotein receptor-related protein (LRP) 1 overactivates the JNK1/2- c -Jun-Fra-2 signaling pathway leading to the induction of α-SMA and periostin expression in human lung fibroblasts (hLF). These changes are accompanied by increased contractility of the cells and the integrin- and protease-dependent release of active transforming growth factor (TGF)-β1 from the extracellular matrix (ECM) stores. Liberation of active TGF-β1 from the ECM further enhances α-SMA and periostin expression thus accelerating the phenotypic switch of hLF. Global gene expression profiling of LRP1-depleted hLF revealed that the loss of LRP1 affects cytoskeleton reorganization, cell-ECM contacts, and ECM production. In line with these findings, fibrotic changes in the skin and lung of Fra-2 transgenic mice were associated with LRP1 depletion and c -Jun overexpression. Altogether, our results suggest that dysregulation of LRP1 expression in fibroblasts in healing tissue may lead to the unrestrained expansion of contractile myofibroblasts and thereby to fibrosis development. Further studies identifying molecules, which regulate LRP1 expression, may provide new therapeutic options for largely untreatable human fibrotic diseases. • Loss of LRP1 in fibroblasts induces α-SMA expression in a JNK/AP-1-dependent manner leading to increased cell contractility. • Elevated contractility of LRP1-deficient cells promotes activation of latent TGF-β1 from the ECM stores. • Augmented TGF-β1 levels accelerate phenotypic switch of LRP1-depleted fibroblasts. • Global gene profiling indicates changes in cytoskeleton reorganization and ECM production in LRP1-deficient fibroblasts. • Fibrotic changes in the skin and lung of Fra-2 transgenic mice are associated with LRP1 loss. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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10. Potential Applications of Flat-Panel Volumetric CT in Morphologic and Functional Small Animal Imaging.
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Greschus, Susanne, Kiessling, Fabian, Lichy, Matthias P., Moll, Jens, Mueller, Margareta M., Savai, Rajkumar, Rose, Frank, Ruppert, Clemens, Günther, Andreas, Luecke, Marcus, Fusenig, Norbert E., Semmler, Wolfhard, and Traupe, Horst
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DIAGNOSTIC imaging , *LUNG cancer , *TOMOGRAPHY , *PULMONARY fibrosis , *PERFUSION , *NONINVASIVE diagnostic tests - Abstract
Noninvasive radiologic imaging has recently gained considerable interest in basic and preclinical research for monitoring disease progression and therapeutic efficacy. In this report, we introduce flat-panel volumetric computed tomography (fpVCT) as a powerful new tool for noninvasive imaging of different organ systems in preclinical research. The three-dimensional visualization that is achieved by isotropic high-resolution datasets is illustrated for the skeleton, chest, abdominal organs, and brain of mice. The high image quality of chest scans enables the visualization of small lung nodules in an orthotopic lung cancer model and the reliable imaging of therapy side effects such as lung fibrosis. Using contrast-enhanced scans, fpVCT displayed the vascular trees of the brain, liver, and kidney down to the subsegmental level. Functional application of fpVCT in dynamic contrast-enhanced scans of the rat brain delivered physiologically reliable data of perfusion and tissue blood volume. Beyond scanning of small animal models as demonstrated here, fpVCT provides the ability to image animals up to the size of primates. [ABSTRACT FROM AUTHOR]
- Published
- 2005
- Full Text
- View/download PDF
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