28 results on '"Su, Shanshan"'
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2. Discovery and structure-activity relationship studies of novel tetrahydro-β-carboline derivatives as apoptosis initiators for treating bacterial infections
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Su, Shanshan, Liu, Hongwu, Zhang, Junrong, Qi, Puying, Ding, Yue, Zhang, Ling, Yang, Linli, Liu, Liwei, Zhou, Xiang, and Yang, Song
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- 2024
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Catalog
3. Impact of childhood trauma on the abnormal functional connectivity of brain regions in the fear network model of panic disorder
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Hong, Ang, Zhou, Shuangyi, Yang, Chen, Liu, Xitong, Su, Shanshan, and Wang, Zhen
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- 2023
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4. A novel spectral index for estimating fractional cover of non-photosynthetic vegetation using near-infrared bands of Sentinel satellite
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Tian, Jia, Su, Shanshan, Tian, Qingjiu, Zhan, Wenfeng, Xi, Yanbiao, and Wang, Ning
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- 2021
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5. A novel graphene oxide-carbon nanotubes anchored α-FeOOH hybrid activated persulfate system for enhanced degradation of Orange II
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Su, Shanshan, Liu, Yuyang, He, Wei, Tang, Xianchun, Jin, Wei, and Zhao, Yaping
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- 2019
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6. Efficient transformation and elimination of roxarsone and its metabolites by a new α-FeOOH@GCA activating persulfate system under UV irradiation with subsequent As(V) recovery
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Su, Shanshan, Cao, Chengjin, Zhao, Yaping, and Dionysiou, Dionysios D.
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- 2019
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7. Association between polyunsaturated fatty acids and progression among patients with diabetic kidney disease.
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Liu, Wu, Liu, Shiyi, Ren, Qiuyue, Yang, Ronglu, Su, Shanshan, and Jiang, Xiaoyu
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Diabetic kidney disease (DKD) is the major complication of diabetes mellitus (DM) and one of the leading causes of end-stage renal disease. Early detection and treatment are contributing to delay the progression of DKD. Dietary management has potential benefits for DKD, especially the intake of polyunsaturated fatty acids (PUFAs). However, there is a lack of sufficient evidence, so we aimed to explore the association between PUFAs intake and DKD progression. In the National Heath and Nutrition Examination Survey (NHANES) between 2011–2018, a cross-sectional study was conducted among adults with T2DM. DKD was diagnosed with urine albumin to creatinine ratio (ACR) ≥ 30 mg/g or estimated glomerular filtration rate (eGFR) <60 ml/min/1.73 m
2 . Using Survey package of R to arrange the collected PUFAs intake data in order from small to large and divide them into four equal parts, which were expressed as Q1, Q2, Q3 and Q4 respectively. To investigate the association between PUFAs intake and DKD, a weighted univariate logistic regression analysis was performed and the odds ratio (OR) and 95% confidence interval (CI) were calculated for the association with DKD and PUFAs quartiles. The study involved 3287 participants with T2DM, including 2043 non-DKD and 1244 DKD patients. The results showed that the intake of PUFAs was a protective factor for DKD (p = 0.022), and with the increase of the PUFAs, renal function improved in DKD patients, the adjusted mean of eGFR and Scr changing from 57 (41, 86) in Q1 to 71 (55, 101) ml/min in Q4 (p 0.001), 103 (73, 131) in Q1 to 90 (68, 117) in Q4 (p = 0.031), respectively. Our study indicated that intake of more PUFAs may contribute to delay DKD progression, while different n-6/n-3 ratios need to be explored to protect the kidney. • Dietary management is a potential therapeutic strategy for diabetic kidney disease. • Polyunsaturated fatty acid intake may delay the progression of diabetic kidney disease. • Exploring the appropriate ratio of n-6/n3 PUFAs is also an important part of dietary management. [ABSTRACT FROM AUTHOR] more...- Published
- 2024
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8. Association of abnormal white matter integrity in the acute phase of motor vehicle accidents with post-traumatic stress disorder
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Hu, Hao, Zhou, Yan, Wang, Qian, Su, Shanshan, Qiu, Yongming, Ge, Jianwei, Wang, Zhen, and Xiao, Zeping
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- 2016
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9. Luteolin ameliorates hypoxic pulmonary vascular remodeling in rat via upregulating KV1.5 of pulmonary artery smooth muscle cells.
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Zhang, Zhaoxia, Chen, Ju, Su, Shanshan, Xie, Xin, Ji, Lei, Li, Zhanqiang, and Lu, Dianxiang
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• Luteolin increased Kv1.5 in lung tissues of HPH rat and PASMCs under hypoxia. • Kv1.5 was involved in regulation of [Ca
2+ ] cyt , proliferation, apoptosis-related protein. • Luteolin ameliorated HPVR via upregulating Kv1.5 of PASMCs. • Luteolin dilated pulmonary artery by activating Kv and K Ca in smooth muscle. Hypoxic pulmonary vascular remodeling (HPVR) is a key pathological feature of hypoxic pulmonary hypertension (HPH). Oxygen-sensitive potassium (K+ ) channels in pulmonary artery smooth muscle cells (PASMCs) play a crucial role in HPVR. Luteolin (Lut) is a plant-derived flavonoid compound with variety of pharmacological actions. Our previous study found Lut alleviated HPVR in HPH rat. To elucidate the mechanism by which Lut mitigated HPVR, focusing on oxygen-sensitive voltage-dependent potassium channel 1.5 (Kv1.5). HPH rat model was established using hypobaric chamber to simulate 5000 m altitude. Isolated perfused/ventilated rat lung, isolated pulmonary arteriole ring was utilized to investigate the impact of Lut on K+ channels activity. Kv1.5 level in lung tissue and pulmonary arteriole of HPH rat was assessed. CyclinD1, CDK4, PCNA, Bax, Bcl-2, cleaved caspase-3 levels in lung tissue of HPH rat were tested. The effect of Lut on Kv1.5, cytoplasmic free calcium concentration ([Ca2+ ] cyt), CyclinD1, CDK4, PCNA, Bax/Bcl-2 was examined in PASMCs under hypoxia, with DPO-1 as a Kv1.5 specific inhibitor. The binding affinity between Lut and Kv1.5 in PASMCs was detected by drug affinity responsive target stability (DARTS). The overexpression of KCNA5 gene (encoding Kv1.5) in HEK293T cells was utilized to confirm the interaction between Lut and Kv1.5. Furthermore, the impact of Lut on mitochondrial structure, SOD, GSH, GSH-Px, MDA and HIF-1α levels were evaluated in lung tissue of HPH rat and PASMCs under hypoxia. Lut dilated pulmonary artery by directly activating Kv and Ca2+ -activated K+ channels (K Ca) in smooth muscle. Kv1.5 level in lung tissue and pulmonary arteriole of HPH rat was upregulated by Lut. Lut downregulated CyclinD1, CDK4, PCNA while upregulating Bax/Bcl-2/caspase-3 axis in lung tissue of HPH rat. Lut decreased [Ca2+ ] cyt , reduced CDK4, CyclinD1, PCNA, increased Bax/Bcl-2 ratio, in PASMCs under hypoxia, by upregulating Kv1.5. The binding affinity and the interaction between Lut and Kv1.5 was verified in PASMCs and in HEK293T cells. Lut also decreased [Ca2+ ] cyt and inhibited proliferation via targeting Kv1.5 of HEK293T cells under hypoxia. Furthermore, Lut protected mitochondrial structure, increased SOD, GSH, GSH-Px, decreased MDA, in lung tissue of HPH rat. Lut downregulated HIF-1α level in both lung tissue of HPH rat and PASMCs under hypoxia. Lut alleviated HPVR by promoting vasodilation of pulmonary artery, reducing cellular proliferation, and inducing apoptosis through upregulating of Kv1.5 in PASMCs. [Display omitted] [ABSTRACT FROM AUTHOR] more...- Published
- 2024
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10. Srolo Bzhtang, a traditional Tibetan medicine formula, inhibits cigarette smoke induced airway inflammation and muc5ac hypersecretion via suppressing IL-13/STAT6 signaling pathway in rats.
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Jing, Linde, Su, Shanshan, Zhang, Dejun, Li, Zhanqiang, Lu, Dianxiang, and Ge, Rili
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ANIMAL experimentation , *BENZOPYRANS , *BRONCHOALVEOLAR lavage , *CELLULAR signal transduction , *CYTOKINES , *ENZYME-linked immunosorbent assay , *GENE expression , *GLYCOPROTEINS , *IMMUNOHISTOCHEMISTRY , *INFLAMMATORY mediators , *INTERLEUKINS , *LUNGS , *MEDICINAL plants , *TIBETAN medicine , *MESSENGER RNA , *MUCUS , *ORAL drug administration , *POLYMERASE chain reaction , *RATS , *SMOKING , *STAINS & staining (Microscopy) , *TRANSCRIPTION factors , *TUMOR necrosis factors , *WESTERN immunoblotting , *PLANT extracts , *REVERSE transcriptase polymerase chain reaction , *DEXAMETHASONE , *INDOLE compounds , *FLUORESCENT dyes , *CHRONIC bronchitis , *PHARMACODYNAMICS - Abstract
Abstract Ethnopharmacological relevance Srolo Bzhtang (SBT), a traditional Tibetan medicine formula, was composed of three herbs, Solms-Laubachia eurycarpa , Bergenia purpurascens , Glycyrrhiza uralensis , and one lac, and was first documented in the ancient Tibetan medical work Four Medical Tantras (rGyud-bzhi) in the eighth century AD. It has been widely used to treat lung "phlegm-heat" syndromes such as chronic bronchitis and chronic obstructive pulmonary disease (COPD). Objective The aim of this study was to evaluate the potential influences of aqueous extract of SBT on airway inflammation and mucus secretion and to reveal the underlying mechanism in a rat model of cigarette smoke (CS)-induced chronic bronchitis (CB). Materials and methods Sixty male Sprague-Dawley rats were randomly divided to six groups: control (room air exposure), model (CS exposure), DEX (CS exposure and 0.2 mg/kg/day dexamethasone), and three SBT (CS exposure and 1.67, 2.50, and 3.34 g/kg/day SBT) groups. DEX and the three doses of SBT were administered by oral gavage every day for eight weeks. Pathological changes and mucus expression in the lung tissue were determined by hematoxylin and eosin (H&E), Alcian blue-periodic acid-Schiff (AB-PAS) and immunohistochemical staining. The levels of cytokines in bronchoalveolar lavage fluid (BALF) were assessed by ELISA. Western blot analysis and qRT-PCR were performed to explore the effects of SBT on the expression of IL-13, STAT6 and MUC5AC. Results Pretreatment with SBT attenuated the TNF-α, IL-8, IL-13 expression levels in BALF and the inflammatory cell infiltration in bronchial walls and peribronchial lung tissue. SBT exhibited a dose-dependent downregulation of MUC5AC expression as assessed by AB-PAS and immunohistochemical staining. The protein and mRNA levels of IL-13, STAT6/p-STAT6 and MUC5AC were also downregulated by SBT preconditioning. Conclusion These results for the first time demonstrated that SBT exhibited protective effects on CS-induced airway inflammation and MUC5AC hypersecretion, which might be related to the downregulation of the IL-13/STAT6 signaling pathway. Graphical abstract fx1 [ABSTRACT FROM AUTHOR] more...
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- 2019
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11. Transformation pathway and degradation mechanism of methylene blue through β-FeOOH@GO catalyzed photo-Fenton-like system.
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Su, Shanshan, Liu, Yuyang, Liu, Xuemin, Jin, Wei, and Zhao, Yaping
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METHYLENE blue , *CHEMICAL decomposition , *GRAPHENE oxide , *CATALYSIS , *NANOPARTICLES , *HYDROLYSIS - Abstract
Abstract To enhance the catalytic and separation properties of akaganéite nanoparticles, rice spike-like akaganéite impregnated graphene oxide (β-FeOOH@GO) nanocomposite was fabricated through facile hydrolysis. The apparent first-order decolorization rate of methylene blue (MB) in β-FeOOH@GO catalyzed photo Fenton-like system was 0.6322 min−1 about 3 folds that of prinstine β-FeOOH nanoparticles. The degradation intermediates of MB adsorbed on the solid surface of β-FeOOH@GO were comprehensively identified with time of flight-secondary ion mass spectroscopy (TOF-SIMS) for the first time. Newly identified sulfoxide intermediates, sulphone intermediates and desulfurization intermediates and N-demethylaton or dedimethamine intermediates were reported for the first time. The proposed degradation pathway of MB predominantly proceeded with the rupture of phenothiazine rings oxided with OH, Image 1 and singlet oxygen (1O 2) radicals, which fully extending the reaction pathways proposed in previous work in literature. The enhanced catalytic activity of β-FeOOH@GO was ascribed to the formation of heterojunctions confirmed by the presence of Fe O C chemical bonds through X-ray photoelectron spectroscopy (XPS). The complete elimination of MB and its acute toxicity to Luminous bacteria showed that β-FeOOH@GO would be served as a highly efficient Fenton-like catalyst for treatment of high concentration refractory organic contaminant. Highlights • β-FeOOH@GO greatly enhanced Fenton oxidation efficiency. • OH, HO 2 − and 1O 2 radicals were involved this system. • Degradation intermediates on catalyst surface was identified with TOF-SIMS. • A series of newly identified intermediates were reported first time. • Synergistic catalytic mechanism of β-FeOOH@GO was elucidated. [ABSTRACT FROM AUTHOR] more...
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- 2019
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12. Tsantan Sumtang, a traditional Tibetan medicine, protects pulmonary vascular endothelial function of hypoxia-induced pulmonary hypertension rats through AKT/eNOS signaling pathway.
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Li, Na, Su, Shanshan, Xie, Xin, Yang, Zhanting, Li, Zhanqiang, and Lu, Dianxiang
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PULMONARY hypertension prevention , *ENDOTHELIUM physiology , *ENDOTHELIAL cells , *IN vitro studies , *ENDOTHELIUM , *BLOOD vessels , *MEDICINAL plants , *IN vivo studies , *HIGH performance liquid chromatography , *LUNGS , *ANIMAL experimentation , *BIOAVAILABILITY , *ORGANIC compounds , *TRADITIONAL medicine , *CELLULAR signal transduction , *RATS , *TRANSFERASES , *CELL proliferation , *MASS spectrometry , *PLANT extracts , *HEMODYNAMICS , *BIOLOGICAL assay , *HYPOXEMIA , *DISEASE complications - Abstract
Tsantan Sumtang (TS), originated from the Four Tantras , is an empirical Tibetan medicine prescription, which has been widely used for treating cardiovascular diseases in the clinic in Qinghai Province of China. Our previous studies found that TS alleviated hypoxia-induced pulmonary hypertension (HPH) in rats. However, the effect and bioactive fractions of TS on hypoxia-injured pulmonary vascular endothelium are unknown. To investigate the effect, bioactive fractions and pharmacological mechanism of TS on hypoxia-injured pulmonary vascular endothelium in vivo and in vitro. In vivo studies, HPH animal model was established, and TS was administrated for four weeks. Then, hemodynamic indexes, ex vivo pulmonary artery perfusion experiment, morphological characteristics, nitric oxide (NO) production, and the protein expression of protein kinase B (AKT)/endothelial nitric oxide synthase (eNOS) and AMP-activated protein kinase (AMPK)/eNOS signaling were determined. In vitro studies, 1% O 2 -induced pulmonary artery endothelial cells (PAECs) injury model was applied for screening bioactive fractions of TS by cell proliferation assay and NO production measurement. The associated proteins of AKT/eNOS signaling were further measured to elucidate underlying mechanism of bioactive fraction of TS via using phosphatidylinositol-3 kinase (PI3K) inhibitor LY294002. Ultra-high performance liquid chromatography with hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS) was used to reveal the chemical profile of bioactive fraction of TS. TS showed protective effect on the integrity of distal pulmonary arterial endothelium in HPH rats. Tsantan Sumtang dilated pulmonary arterial rings in HPH rats. TS enhanced NO bioavailability in lung tissue via regulating AKT/eNOS signaling. Furthermore, in the cellular level, cell viability as well as NO content of hypoxia-injured PAECs were elevated by fraction 17 of water extract of TS (WTS), through activating the AKT/eNOS signaling. Ellagic acid could be one of compositions in fraction 17 of WTS to produce NO in hypoxia-injured PAECs. TS restored pulmonary arterial endothelial function in HPH rats. The bioactive fraction 17 was screened, which protected hypoxia-injured PAECs via upregulating AKT/eNOS signaling. [Display omitted] • TS attenuated HPH by protecting pulmonary vascular endothelial function. • Fraction 17 of WTS elevated hypoxia-injured PAECs viability as well as NO content. • TS activated AKT/eNOS pathway in lung tissue of HPH rats and 1% O 2 -injured PAECs. • Ellagic acid identified in fraction 17 produced NO in 1% O 2 -injured PAECs. [ABSTRACT FROM AUTHOR] more...
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- 2024
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13. Bioactive fraction of Rhodiola algida against chronic hypoxia-induced pulmonary arterial hypertension and its anti-proliferation mechanism in rats.
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Nan, Xingmei, Su, Shanshan, Ma, Ke, Ma, Xiaodong, Wang, Ximeng, Zhaxi, Dongzhu, Ge, Rili, Li, Zhanqiang, and Lu, Dianxiang
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CELL proliferation , *ANIMAL experimentation , *HYPOXEMIA , *BLOOD pressure , *GENE expression , *RIGHT heart ventricle , *HEMATOCRIT , *HYPERTROPHY , *LIQUID chromatography , *MASS spectrometry , *TIBETAN medicine , *POLYMERASE chain reaction , *PULMONARY artery , *PULMONARY hypertension , *RATS , *WESTERN immunoblotting , *PLANT extracts , *NUCLEAR proteins , *CELL cycle proteins , *DISEASE complications - Abstract
Ethnopharmacological relevance Rhodiola algida var. tangutica (Maxim.) S.H. Fu is a perennial plant of the Crassulaceae family that grows in the mountainous regions of Asia. The rhizome and roots of this plant have been long used as Tibetan folk medicine for preventing high latitude sickness. Aim of the study The aim of this study was to determine the effect of bioactive fraction from R. algida (ACRT) on chronic hypoxia-induced pulmonary arterial hypertension (HPAH) and to understand the possible mechanism of its pharmacodynamic actions. Materials and methods Male Sprague-Dawley rats were separated into five groups: control group, hypoxia group, and hypoxia+ACRT groups (62.5, 125, and 250 mg/kg/day of ACRT). The chronic hypoxic environment was created in a hypobaric chamber by adjusting the inner pressure and oxygen content for 4 weeks. After 4 weeks, major physiological parameters of pulmonary arterial hypertension such as mPAP, right ventricle index (RV/LV+S, RVHI), hematocrit (Hct) levels and the medial vessel thickness (wt%) were measured. Protein and mRNA expression levels of proliferating cell nuclear antigen (PCNA), cyclin D1, p27Kip1 and cyclin-dependent kinase 4 (CDK4)) were detected by western blotting and real time PCR respectively. Chemical profile of ACRT was revealed by ultra performance liquid chromatography coupled with quadrupole time of flight mass spectrometry (UHPLC-Q-TOF-MS/MS). Results The results showed that a successful HPAH rat model was established in a hypobaric chamber for 4 weeks, as indicated by the significant increase in mPAP, RV/LV+S, RV/BW and wt%. Compared with the normal group, administration of ACRT reduced mPAP, right ventricular hypertrophy, pulmonary small artery wall thickness, and damage in ultrastructure induced by hypoxia in rats. PCNA, cyclin D1, and CDK4 expression was reduced ( p <0.05), and p27Kip1 expression increased ( p <0.05) in hypoxia+ACRT groups compared to hypoxia. 38 constituents in bioactive fraction were identified by UHPLC-Q-TOF-MS/MS. Conclusion Our results suggest that ACRT could alleviate chronic hypoxia-induced pulmonary arterial hypertension. And its anti-proliferation mechanism in rats based on decreasing PCNA, cyclin D1, CDK4 expression level and inhibiting p27Kip1 degradation. [ABSTRACT FROM AUTHOR] more...
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- 2018
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14. Computational method for rapid screening of the metabolites of Pulsatilla chinensis in rats using UHPLC-Q-TOF/MS combined with mass spectrum-based orthogonal projection.
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Su, Shanshan, Xue, Guiren, Shang, Jiawei, Yan, Pengfei, Wang, Jianxin, Yan, Chengye, Li, Jiaxi, Xiong, Xue, and Xu, Huijun
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ORTHOGRAPHIC projection , *TIME-of-flight mass spectrometry , *MEDICAL screening , *METABOLITES , *DEHYDRATION reactions , *BILE , *HIGH performance liquid chromatography - Abstract
Screening metabolites in vivo can be challenging due to the complexity of traditional Chinese medicine (TCM) and the ambiguous intracorporal process. To resolve this problem, we established the mass spectrum-based orthogonal projection (MSOP) method to differentiate prototype compounds from metabolites in vivo and applied it to the study of metabolites of Pulsatilla chinensis (PC). Initially, the validity and feasibility of the MSOP method were verified by using the ultra- high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) data of reference solution. Then, the MSOP method was applied to screen the metabolites of PC. A total of 63 metabolites were identified in vivo (urine, feces, bile, and plasma samples) and in vitro (intestinal bacteria biological sample). The results indicated that the main metabolic pathways of pentacyclic triterpenoids were demethylation, oxidation, dehydration, sulfation, and glucuronidation reactions. This study contributes to developing an integrated strategy based on chemometrics to characterize and classify the metabolism feature of pentacyclic triterpenoids of PC. This will support the scientific and rational application of PC in the clinic. The MSOP method based on the orthogonality of MS signals was used to differentiate the prototype compounds from metabolites in vivo. The method provides scientific and reliable support for fully understanding the metabolic fate of TCM. [Display omitted] • The orthogonal projection was used to screen the metabolites rapidly. • Analyzing the metabolic feature by using UHPLC-Q-TOF/MS and orthogonal projection. • A total of 20 prototype components and 63 metabolites were identified. • The study affords support for understanding the metabolic fate of Chinese medicine. [ABSTRACT FROM AUTHOR] more...
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- 2023
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15. In vitro characterization of a (E)-β-farnesene synthase from Matricaria recutita L. and its up-regulation by methyl jasmonate.
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Su, Shanshan, Liu, Xueyan, Pan, Guifang, Hou, Xiaojuan, Zhang, Huimin, and Yuan, Yi
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FARNESENE , *GERMAN chamomile , *SEMIOCHEMICALS , *SESQUITERPENES , *CHINESE cabbage , *IN vitro studies ,APHID control - Abstract
(E)-β-farnesene is a sesquiterpene semiochemical that is used extensively by both plants and animals for communication. This acyclic olefin is found in the essential oil of chamomile ( Matricaria recutita ) and was demonstrated that it could attract natural enemies to reduce cabbage aphids in the Chinese cabbage fields. However, little is known regarding the sequence and function of (E)-β-farnesene synthase in M. recutita . In this study, we reported a new full-length cDNA encoding (E)-β-farnesene synthase from M. recutita ( Mr - βFS ). The cDNA of Mr - βFS consisted of 2010 bp including 1725 bp of coding sequence encoding a protein of 574 amino acids with a molecular weight of 67 kDa. The deduced amino acid sequence exhibits a considerably higher homology to βFS from Artemisia annua (about 92% identity) than to βFSs from other plants (about 20–40% identity). The recombinant enzyme, produced in Escherichia coli , catalyzed the formation of a single product, (E)-β-farnesene, from farnesyl diphosphate. Real-time quantitative PCR (qRT-PCR) analysis showed that Mr - βFS expression was highest in leaves and lowest in disk florets. The treatment of M. recutita with methyl jasmonate (MeJA) significantly enhanced the transcriptional level of βFS gene and the content of (E)-β-farnesene in M. recutita . The transcriptional level of βFS gene was approximately 11.5-fold higher than the control sample and the (E)-β-farnesene emission level ranged from approximately from 0.082 to 0.695 μg/g after 24 h induction. Our results laid a solid foundation for later improving crop aphid resistance by transgenic technology and provided an important basic data for the regulation of valuable products from M. recutita . [ABSTRACT FROM AUTHOR] more...
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- 2015
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16. Plasma brain-derived neurotrophic factor levels in patients suffering from post-traumatic stress disorder.
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Su, Shanshan, Xiao, Zeping, Lin, Zhiguang, Qiu, Yongming, Jin, Yichao, and Wang, Zhen
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PLASMA gases , *BRAIN-derived neurotrophic factor , *TREATMENT of post-traumatic stress disorder , *TRAFFIC accidents -- Psychological aspects , *NEUROPSYCHIATRY , *FOLLOW-up studies (Medicine) - Abstract
A number of studies have been done to investigate the role of brain-derived neurotrophic factor (BDNF) in patients with post-traumatic stress disorder (PTSD). In this study we aimed to test the relationship between plasma BDNF levels and PTSD. We solicited 65 subjects having recently experienced road traffic accidents (RTA) conforming to screening criteria. They were given follow-up examinations after one month, three months, and six months. PTSD was diagnosed according to the Diagnostic and Statistical Manual of Mental Disorders (DSM-IV-R-TR, American Psychiatric Association, 2000) using the Mini International Neuropsychiatric Interview (MINI). All participants were divided into two groups: a group with PTSD and a group without PTSD. There were no significant differences in plasma BDNF levels between the two groups at either the 48 h or six-month examination. Within the PTSD group, no significant differences were found in plasma BDNF levels between the two examinations. BDNF levels in those without PTSD showed a higher trend over time after trauma. Higher BDNF levels may be an important protective factor for the prevention of traumatized subjects from developing PTSD. [ABSTRACT FROM AUTHOR] more...
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- 2015
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17. Srolo Bzhtang reduces inflammation and vascular remodeling via suppression of the MAPK/NF-κB signaling pathway in rats with pulmonary arterial hypertension.
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Chen, Tingting, Su, Shanshan, Yang, Zhanting, Zhang, Dejun, Li, Zhanqiang, and Lu, Dianxiang
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INFLAMMATION prevention , *CYTOKINES , *INTERLEUKINS , *ANIMAL experimentation , *WESTERN immunoblotting , *HEALTH outcome assessment , *CELLULAR signal transduction , *RATS , *GENE expression , *TUMOR necrosis factors , *DESCRIPTIVE statistics , *PLANT extracts , *MITOGEN-activated protein kinases , *VASCULAR remodeling , *HYPOXEMIA ,THERAPEUTIC use of plant extracts - Abstract
Srolo Bzhtang (SBT), which consists of Solms-laubachia eurycarpa , Bergenia purpurascens , Glycyrrhiza uralensis , and lac secreted by Laccifer lacca Kerr (Lacciferidae Cockerell), is a well-known traditional Tibetan medicinal formula and was documented to cure "lung-heat" syndrome by eliminating "chiba" in the ancient Tibetan medical work Four Medical Tantras (Rgyud bzhi). Clinically, it is a therapy for pulmonary inflammatory disorders, such as pneumonia, chronic bronchitis, and chronic obstructive pulmonary disease. However, whether and how SBT participates in pulmonary arterial hypertension (PAH) is still unclear. We aimed to determine the role of SBT in attenuating pulmonary arterial pressure and vascular remodeling caused by monocrotaline (MCT) and hypoxia. To elucidate the potential mechanism underlying SBT-mediated PAH, we investigated the changes in inflammatory cytokines and mitogen-activated protein kinase (MAPK)/nuclear factor-kappa B (NF-κB) signaling pathway. MCT- and hypoxia-induced PAH rat models were used. After administering SBT for four weeks, the rats were tested for hemodynamic indicators, hematological changes, pulmonary arterial morphological changes, and the levels of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in serum and lung tissues. Protein expression of the MAPK/NF-κB signaling pathway was determined using western blotting. SBT reduced pulmonary arterial pressure, vascular remodeling, and the levels of inflammatory cytokines induced by MCT and hypoxia in rats. Furthermore, SBT significantly suppressed the MAPK/NF-κB signaling pathway. To our knowledge, this is the first study to demonstrate that SBT alleviates MCT- and hypoxia-induced PAH in rats, which is related to its anti-inflammatory actions involving inhibition of the MAPK/NF-κB signaling pathway. [Display omitted] [ABSTRACT FROM AUTHOR] more...
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- 2022
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18. Integrative analyses of widely targeted metabolomic profiling and derivatization-based LC-MS/MS reveals metabolic changes of Zingiberis Rhizoma and its processed products.
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Xue, Guiren, Su, Shanshan, Yan, Pengfei, Shang, Jiawei, Wang, Jianxin, Yan, Chengye, Li, Jiaxi, Wang, Qiao, Xiong, Xue, and Xu, Huijun
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METABOLOMICS , *LIQUID chromatography-mass spectrometry , *DAUGHTER ions , *NUTRITIONAL value , *METABOLITES - Abstract
• Widely targeted metabolomic technology was applied for differences in ZR, ZRP and CG. • Chemical derivatization was used to extract the different submetabolomes. • Most metabolites contributed to a downward trend after processing of ZR. • The maximum content of flavanones, phenylpropanes and polyphenols appeared in ZRP. • Alcohols represented the maximum content in CG comparing with ZR and ZRP. Zingiberis Rhizoma (ZR) has nutritional value and application potentiality, while Zingiberis Rhizoma Praeparatum (ZRP) and Carbonised Ginger (CG) are two main processed products of ZR based on different methods. Here, we performed a widely targeted metabolomics method with Sequential Windowed Acquisition of all Theoretical fragment ions (SWATH) mode to analyze differential metabolites in ZR, ZRP and CG. Additionally, the chemical derivatization was applied to characterize different submetabolomes and improve the separation effect and MS response of metabolites. In total, 369 metabolites were identified and divided into 14 categories, 104 of which were differential metabolites. Our results suggest that carbohydrates, nucleotides, organic acids, vitamins, lipids, indoles, alkaloids, and terpenes contributed to a downward trend after processing, but the maximum content of flavanones, phenylpropanes and polyphenols appeared in ZRP, and that of alcohols appeared in CG. These findings serve as promising perspectives for developing functional food in ZR, ZRP and CG. [ABSTRACT FROM AUTHOR] more...
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- 2022
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19. Luteolin ameliorates hypoxia-induced pulmonary hypertension via regulating HIF-2α-Arg-NO axis and PI3K-AKT-eNOS-NO signaling pathway.
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Ji, Lei, Su, Shanshan, Xin, Mingyuan, Zhang, Zhaoxia, Nan, Xingmei, Li, Zhanqiang, and Lu, Dianxiang
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Background: Pulmonary hypertension (PH) is a devastating disease with poor prognosis and high mortality. Hypoxia induced pulmonary hypertension (HPH) is a persistent threat to human health, especially to people who live on high altitude plateau. Pulmonary vascular endothelial cell is involved in numerous pathophysiological processes, including in vasoconstriction, oxidative stress, cell growth and differentiation. Endothelial cells (ECs) are the first layer to be exposed to changed oxygen levels and hypoxia could lead to ECs dysfunction. Endothelial-derived nitric oxide (NO) is the most important bioactive molecule, which could regulate endothelial homeostasis. PH pathophysiology has been linked to the disruption of NO pathways.Purpose: Luteolin is a kind of plant active ingredient with multiple pharmacological activities. The purpose of this study is to detect the effect of luteolin on HPH with in vivo, ex vivo and in vitro analyses and to further elucidate luteolin's pharmaceutical mechanism with NO related signaling pathway regulation.Methods: Hypobaric chamber was used to establish HPH animal model. Rats were intragastrically administrated luteolin for 28 days. Then hemodynamic indexes, histopathological changes, pulmonary artery endothelial function, NO content and arginase activity in lung tissue, NO related pathway proteins expression were measured to evaluate the effect of luteolin on HPH. PAECs were treated with 1% O2 and incubated with or without luteolin. PAECs vitality, NO content in cells supernatant, and NO related pathway proteins expression were tested to reveal the protective mechanism of luteolin.Results: Luteolin decreased mean pulmonary hypertension of HPH rats, alleviated right ventricular and pulmonary vascular remodeling. Immunofluorescence staining (vWF), isolated perfused/ventilated rat lung experiment indicated that luteolin protected pulmonary vascular endothelial function of HPH rats. Luteolin increased NO content in PAECs supernatant while decreased NO level in lung tissues of HPH rats. Further, it was demonstrated that luteolin inhibited HIF-2α-Arg axis in PAECs and HPH rats. PI3K-AKT-eNOS signaling pathway was upregulated in PAECs, but which was downregulated in lung tissues of HPH rats. Pharmacological effect of luteolin was equivalent or better than sildenafil.Conclusion: Luteolin ameliorated HPH in rats by protecting pulmonary vascular endothelial function via regulating HIF-2α-Arg-NO axis and PI3K-AKT-eNOS-NO signaling pathway. This study may provide a novel perspective and approach to alleviate the devastating disease of HPH. [ABSTRACT FROM AUTHOR] more...- Published
- 2022
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20. Quality control of Zingiberis Rhizoma and its processed products by UHPLC-Q-TOF/MS-based non-targeted metabonomics combining with SIBDV method.
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Xue, Guiren, Su, Shanshan, Yan, Pengfei, Shang, Jiawei, Wang, Jianxin, Yan, Chengye, Li, Jiaxi, Wang, Qiao, Du, Yingfeng, Cao, Liang, and Xu, Huijun
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QUADRUPOLE ion trap mass spectrometry , *QUALITY control , *TIME-of-flight mass spectrometry , *AMINO acid metabolism , *DISCRIMINANT analysis , *PRINCIPAL components analysis , *MULTIVARIATE analysis , *DIETARY supplements - Abstract
[Display omitted] • A novel non-targeted metabonomics method was applyed in discrimination of ZR, ZRP and CG. • A comprehensive SIBDV method was firstly proposed to characterize and screening gingerols in ZR, ZRP and CG. • A total of 59 gingerols were identified, which was the first comprehensive and systematic presentation of gingerols. • PCA and OPLS-DA showed differences, and 33 differential components were characterized as potentially chemical markers. • These findings provides promising perspectives in the quality control of ZR, ZRP and CG. Zingiberis Rhizoma (ZR) is a homologous plant with pungent tastes and aromas, which has unique nutritional value and tremendous application potentiality. Zingiberis Rhizoma Praeparatum (ZRP) and Carbonised Ginger (CG) are processed products of ZR through different processing methods, and they are commonly used ingredients in food supplements. This study used ZR, ZRP and CG from different batches to further understand composition differences after processing. Additionally, we performed non-targeted metabolomics-based profiling of gingerols by ultra-high-performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) in combination with multivariate analysis and compounds identification. In which, we developed a comprehensive SWATH-IDA bi-directionally verified (SIBDV) method integrating the advantages of Sequential Windowed Acquisition of all Theoretical fragment ions (SWATHTM) and traditional information-dependent acquisition (IDA) mode for characterization of gingerols. Potential chemical markers were selected by principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) of chemometrics methods. After that, the threshold variable importance in projection (VIP) value and P value were employed to screen the valuable MS features for discriminating ZR, ZRP and CG. In total, 59 gingerols in the different samples were structurally identified. Results allowed the selection of 33 gingerols, which are nominated as novel markers for materials authentication in ZR, ZRP and CG. The analysis of the study showed that the content of gingerols showed a downward trend after processing, but shogaols and gingerone compounds had an upward trend, resulting in differences in application and pharmacodynamic efficacy. These findings provide promising perspectives in the quality control of ZR, ZRP and CG, as well as for laying the foundation in food design and development. [ABSTRACT FROM AUTHOR] more...
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- 2022
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21. Tsantan Sumtang attenuated chronic hypoxia-induced right ventricular structure remodeling and fibrosis by equilibrating local ACE-AngII-AT1R/ACE2-Ang1-7-Mas axis in rat.
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Dang, Zhancui, Su, Shanshan, Jin, Guoen, Nan, Xingmei, Ma, Lan, Li, Zhanqiang, Lu, Dianxiang, and Ge, Rili
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ANGIOTENSIN converting enzyme , *ANIMAL experimentation , *BLOOD pressure , *PHYSICAL & theoretical chemistry , *ENZYME-linked immunosorbent assay , *GENE expression , *RIGHT heart ventricle , *HEART beat , *IMMUNOHISTOCHEMISTRY , *MESSENGER RNA , *PULMONARY fibrosis , *PULMONARY hypertension , *RATS , *STAINS & staining (Microscopy) , *TRADITIONAL medicine , *WESTERN immunoblotting , *PLANT extracts , *VENTRICULAR remodeling - Abstract
Tsantan Sumtang, which consists of Choerospondias axillaris (Roxb.) Burtt et Hill, Myristica fragrans Houtt and Santalum album L, is a traditional and common prescription of Tibetan medicine. Tsantan Sumtang originates from Four Tantra with properties of nourishing heart and has been used as a folk medicine for cardiovascular diseases and heart failure in Qinghai, Tibet and Inner Mongolia. Our previous studies found that Tsantan Sumtang showed beneficial effects on right ventricular structure in hypoxia rats, while the underling mechanism remains unclear. To elucidate the underlying mechanisms of Tsantan Sumtang attenuated right ventricular (RV) remodeling and fibrosis of chronic hypoxia-induced pulmonary arterial hypertension (HPAH) rats. Fifty male Sprague Dawley (SD) rats (170 ± 20 g) were randomly divided into control group, hypoxia group, and hypoxia + Tsantan Sumtang groups (1.0 g· kg−1·day−1, 1.25 g· kg−1·day−1, 1.5 g ·kg−1·day−1). Rats in the hypoxia group and hypoxia + Tsantan Sumtang groups were maintained in a hypobaric chamber by adjusting the inner pressure and oxygen content to simulate an altitude of 4500 m for 28 days. The mean pulmonary arterial pressure (mPAP), right ventricle hypertrophy index (RVHI), the ratio of RV weight to tibia length (TL) (RV/TL), heart rate (HR) and RV systolic pressure (RVSP) was determined. Histomorphological assay of RV structure was evaluated by hematoxylin and eosin (HE) staining. RV tissue fibrosis was assessed by collagen proportion area (CPA), collagen I, collagen III and hydroxyproline content. CPA was obtained by picro-sirius red staining (PSR). The expression of collagen I and collagen III were detected by immunohistochemistry and western blotting. The hydroxyproline content was detected by alkaline hydrolysis. In addition, the level of angiotensin II (AngII) and angiotensin 1-7 (Ang1-7) in RV tissue was tested by enzyme-linked immune sorbent assay (ELISA). Protein expression of angiotensin-converting enzyme (ACE), AngII, AngII type 1 receptor (AT1R), angiotensin-converting enzyme 2 (ACE2), Mas receptor (Mas) were determined by immunohistochemistry and western blotting. mRNA level of ACE, AT1R, ACE2, Mas were tested by qPCR. The chemical profile of Tsantan Sumtang was revealed by UHPLC-Q-Exactive hybrid quadrupole-orbitrap mass analysis. Our results showed that RVHI, RV/TL and RVSP were significantly increased in HPAH rat. Furthermore, levels of collagen I, collagen III and hydroxyproline were up-regulated in RV tissue under hypoxia. We found that RV hypertrophy and fibrosis were associated with increased expression of ACE, AngII, AT1R as well as decreased expression of ACE2, Ang1-7 and Mas. RV remodeling and fibrosis were attenuated after Tsantan Sumtang administration by up-regulating ACE2 and Mas level as well as down-regulating ACE, AngII and AT1R levels in RV tissue. 35 constituents in Tsantan Sumtang were identified. Tsantan Sumtang attenuated RV remodeling and fibrosis in rat exposed to chronic hypoxia. The pharmacological effect of Tsantan Sumtang was based on equilibrating ACE-AngII-AT1R and ACE2-Ang1-7-Mas axis of RV tissue in HPAH rat. Image 1 [ABSTRACT FROM AUTHOR] more...
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- 2020
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22. Corrigendum to "Risk factors of lymph node metastasis in the diffuse sclerosing variant of papillary thyroid carcinoma compared with conventional papillary thyroid carcinoma in pediatric populations" [160 (2025) 107120/Article:OO_107120].
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Liu, Yanan, Fu, Nantao, Liu, Haitao, Su, Shanshan, Yang, Tingting, Long, Ping, Zhong, Wei, and Min, Xiang
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CHILD patients , *PAPILLARY carcinoma , *THYROID cancer , *LYMPHATIC metastasis , *SCHOLARLY periodical corrections - Published
- 2025
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23. Risk factors of lymph node metastasis in the diffuse sclerosing variant of papillary thyroid carcinoma compared with conventional papillary thyroid carcinoma in pediatric populations.
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Liu, Yanan, Fu, Nantao, Liu, Haitao, Su, Shanshan, Yang, Tingting, Long, Ping, Zhong, Wei, and Min, Xiang
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RECURRENT laryngeal nerve , *CHILD patients , *LYMPHATIC metastasis , *PAPILLARY carcinoma , *THYROID cancer , *THYROIDECTOMY - Abstract
• The first to analyze data retrospectively from pediatric PTC patients in a large medical unit with a particular focus on LNM patterns. • Comparative analysis between DSV-PTC and C-PTC focus on pediatric population for the first time. • Explored the risk factors associated with LNM in C-PTC, providing valuable insights for clinical practice. To analyze the clinicopathological features and predictors of lymph node metastasis (LNM) in the diffuse sclerosing variant of papillary thyroid carcinoma (DSV-PTC) and compare these findings with conventional papillary thyroid carcinoma (C-PTC) in pediatric populations. Patients aged 18 years or younger who were preoperatively diagnosed with PTC and underwent thyroidectomy at the First Affiliated Hospitals of Nanchang University from January 2017 to May 2024 were included in this study. Demographic and clinicopathological characteristics were retrospectively analyzed. Univariate and multivariate analyses were performed to identify risk factors for lymph node metastasis. Clinical outcomes, including nerve injury, hypocalcemia, and recurrence, were also recorded and evaluated. A total of 119 children were enrolled in this study, with a median age of 15.6 years. Of these, 89 (74.8 %) were female, and 18 (15.1 %) were diagnosed with DSV-PTC. Central lymph node metastasis (CLNM) was observed in 94 (79 %) patients, while 60 (50.4 %) had lateral lymph node metastasis (LLNM). DSV-PTC was associated with a higher incidence of CLNM and LLNM compared to C-PTC (p = 0.039, p < 0.001). Multifocality and bilateral tumors were also more common in DSV-PTC (p < 0.001). Cox regression analysis identified tumor size as an independent predictive factor for CLNM in pediatric C-PTC patients. Tumor size, ETE and the presence of CLNM were independent predictive factors for LLNM. After a mean follow-up of 32.8 months, no patient died from the disease, but four C-PTC patients (4.0 %) developed local recurrences, which were managed surgically. Lung metastasis occurred in 2 (11.1 %) DSV-PTC and 1(1.0 %)C-PTC patients, respectively. Permanent injuries included one case of unilateral recurrent laryngeal nerve(RLN) injury and two cases of bilateral RLN injury. Four patients (4.0 %) experienced temporary hypocalcemia, which resolved after 6 months of calcium therapy. DSV-PTC presents unique characteristics of lymph node metastasis and may be more aggressive than conventional PTC in pediatric populations. The presence of CLNM, tumor size and ETE were identified as independent predictors of LLNM in pediatric patients with conventional PTC. Evaluating these prognostic factors may help in customizing individualized surgical approaches for children, thereby reducing unnecessary surgical complications. [ABSTRACT FROM AUTHOR] more...
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- 2025
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24. Effect of 7-ketocholesterol incorporation on substrate binding affinity and turnover rate of the organic cation transporter 2 (OCT2).
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Xiu, Fangrui, Console, Lara, Indiveri, Cesare, Su, Shanshan, Wang, Tong, and Visentin, Michele
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ORGANIC cation transporters , *LIPID rafts , *HYDROXYCHOLESTEROLS , *CELL membranes , *MOLECULAR docking , *BINDING energy , *PHYTOSTEROLS - Abstract
[Display omitted] The organic cation transporter 2 (OCT2) is pivotal in the renal elimination of several positively charged molecules. OCT2 mode of transport is profoundly influenced by the level of membrane cholesterol. The aim of this study was to investigate the effect of oxidized cholesterol on OCT2 transport activity in human embryonic kidney 293 cells stably transfected with OCT2 (OCT2-HEK293) and in primary renal proximal tubular epithelial cells (RPTEC). Cholesterol was exchanged with 7-ketocholesterol, the main product of cholesterol auto-oxidation, by exposing cells to sterol-saturated methyl-β-cyclodextrin (mβcd). After a 30 min-exposure, approximately 50% of the endogenous cholesterol was replaced by 7-ketocholesterol without significant changes in total sterol level. In the presence of 7-ketocholesterol, [3H]1-methyl-4-phenylpyridinium (MPP+) uptake was significantly reduced in both cell lines. 7-ketocholesterol incorporation did not affect lipid raft integrity, nor OCT2 surface expression and spatial organization. The inhibitory effect of 7-ketocholesterol on MPP+ uptake was abolished by the presence of MPP+ in the trans -compartment. In the presence of 7-ketocholesterol, both K t and V max of MPP+ influx decreased. Molecular docking using OCT2 structure in outward occluded conformation showed overlapping poses and similar binding energies between cholesterol and 7-ketocholesterol. The thermal stability of OCT2 was not changed when cholesterol was replaced with 7-ketocholesterol. We conclude that 7-ketocholesterol confers a higher rigidity to the carrier by reducing its conformational entropy, arguably as a result of changes in plasma membrane physical properties, thereby facilitating the achievement of a higher affinity state at the expense of the mobility and overall cycling rate of the transporter. [ABSTRACT FROM AUTHOR] more...
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- 2024
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25. Commensurate lattice constant dependent thermal conductivity of misoriented bilayer graphene.
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Li, Chenyang, Debnath, Bishwajit, Tan, Xiaojian, Su, Shanshan, Xu, Kui, Ge, Supeng, Neupane, Mahesh R., and Lake, Roger K.
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DENSITY functional theory , *THERMAL conductivity , *PHONONS , *LATTICE constants , *BRILLOUIN zones - Abstract
Misorientation of two layers of bilayer graphene leaves distinct signatures in the electronic properties and the phonon modes. The effect on the thermal conductivity has received the least attention and is the least well understood. In this work, the in-plane thermal conductivity of misoriented bilayer graphene (m-BLG) is investigated as a function of temperature and interlayer misorientation angle using nonequilibrium molecular dynamics (NEMD). The central result is that the calculated thermal conductivities decrease approximately linearly with the increasing lattice constant of the commensurate m-BLG unit cell. Comparisons of the phonon dispersions show that misorientation has negligible affect on the low-energy phonon frequencies and velocities. However, the larger periodicity of m-BLG reduces the Brillouin zone size to the extent that the zone edge acoustic phonons are thermally populated. This allows Umklapp scattering to reduce the lifetimes of the phonons contributing to the thermal transport, and consequently, to reduce the thermal conductivity. This explanation is supported by direct calculation of reduced phonon lifetimes in m-BLG based on density functional theory (DFT). [ABSTRACT FROM AUTHOR] more...
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- 2018
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26. Metabolomics analysis reveals the physiological mechanism underlying growth restriction in maize roots under continuous negative pressure and stable water supply.
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Zhang, Jili, Wang, Peng, Long, Huaiyu, Su, Shanshan, Wu, Yige, and Wang, Hongrong
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PHYSIOLOGY , *WATER supply , *WATER pressure , *ORGANIC acids , *METABOLOMICS , *METABOLITES , *TREHALOSE , *CORNSTARCH - Abstract
Metabolomics analysis was used to elucidate the mechanism underlying response of the maize root system to continuous negative pressure and stable water supply. Results showed a significant reduction in continuous negative pressure and stable water supply (NPI) relative to intermittent positive pressure water supply (DI), maize root dry matter accumulation and root shoot ratio in NPI was 20.22%, 34.15% and lower than DI, respectively, yield was 44.10% higher than DI, and superoxide anion, malonic dialdehyde, osmotic adjustment substance content, and antioxidant enzyme activity was reduced. Next, we applied liquid chromatography-mass spectrometry, gas chromatography-mass spectrometry, and nuclear magnetic resonance to perform non-targeted metabolomics analysis with the aim of elucidating the physiological basis of root redundant growth restriction. Results revealed presence of 33 different metabolites in NPI, belonging to different categories, including organic acids and their derivatives, nucleosides, nucleotides and their corresponding analogues, lipids and lipid-like molecules, as well as organic oxygen compounds. Moreover, all these were enriched in 44 metabolic pathways, relative to DI. Notably, metabolic pathways, biosynthesis of secondary metabolites, starch and sucrose metabolism, plant hormones biosynthesis, and biosynthesis of plant secondary metabolites might restrict maize roots growth. In NPI was, Trehalose, 2'-deoxyadenosine, isoleucine and ajmaline were 0.34-, 0.05-, 0.004- and 0.17-fold lower than DI, whereas glutamic acid, glutamine and zeatin were 4.62,4.26,4.06 times higher than DI. This might inhibit root redundant growth. In summary, continuous negative pressure and stable water supply inhibited maize root redundant growth restriction by suppressing sugar metabolism and protein synthesis via genetic information transmission. • Root metabolic pathways changed. • Sugar and hormone change inhibit root growth. • Sucrose and zeatin is the key metabolites. [ABSTRACT FROM AUTHOR] more...
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- 2022
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27. Miniaturized solid-phase extraction using a mesoporous molecular sieve SBA-15 as sorbent for the determination of triterpenoid saponins from Pulsatilla chinensis by ultrahigh-performance liquid chromatography-charged aerosol detection.
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Jiang, Honghong, Zhang, Wendan, Yang, Jianxi, Xue, Guiren, Su, Shanshan, Li, Cheng, Wang, Qiao, Guo, Wei, and Xu, Huijun
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SOLID phase extraction , *TRITERPENOID saponins , *MOLECULAR sieves , *MESOPOROUS materials , *CHINESE medicine , *AEROSOLS , *COMPLEX compounds - Abstract
TOC The flowchart of the proposal method. • The solid phase extraction method was developed and optimized using molecular sieve SBA-15 as an effective sorbent. • The possible adsorption mechanism of triterpenoid saponins loading to SBA-15 was illuminated. • The proposed method was applied to the pre-concentration and determination of triterpenoid saponins for the first time. • The study provided theoretical basis and data support for the development of mesoporous material in the field of SPE. • This study provided a feasible method for studying complex Chinese medicines system with no UV activity. A simple, sensitive and efficient solid-phase extraction method, combined with ultrahigh-performance liquid chromatography-charged aerosol detection, was applied to the pre-concentration and determination of four triterpenoid saponins from Pulsatilla chinensis (P. chinensis) ultrasound extract samples. Mesoporous molecular sieve Santa Barbara Amorphous 15 (SBA-15) displayed higher selectivity compared to Mobil Composition of Matter 41 (MCM-41), NH 2 -SBA-15 and mesoporous carbon when it comes to being used in pretreatment methods. It was applied as an effective sorbent in the SPE for the enrichment of the target analytes. Additionally, several key experimental parameters including the kinds of sorbents, the amount of SBA-15, the elute pH and types of elution solvent were investigated in detail. Under the optimized conditions, the satisfactory linearity (r2 ≥ 0.9940) was acquired and the limits of detection reached 0.461−0.976 μg/mL for the target analytes. The recoveries ranged from 95.1%–103.2%. The experimental results showed that SBA-15 was a candidate material for the purification and concentration of target triterpenoid saponins from complex P. chinensis samples. The study provided theoretical support for the application of mesoporous materials in the field of drug separation and provided references for the extraction and determination of trace compounds in the complex systems of traditional Chinese medicine. [ABSTRACT FROM AUTHOR] more...
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- 2021
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28. A new phenylpropanoid glycoside from the bark of Streblus ilicifolius (Vidal) Corner.
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Zhang, Gaorong, Hao, Lili, Zhou, Dexiong, Liu, Wei, Li, Chenguo, Su, Shanshan, Xu, Xia, Huang, Xishan, and Li, Jun
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GLYCOSIDES , *BARK - Abstract
A new compound, pheglycoside A (1), along with four known aromatic glycosides (2-5) and three known lignan glycosides (6 – 8) were isolated from Streblus ilicifolius (Vidal) Corner. The structure of compound 1 was determined by spectral analyses, including HRESIMS, 1D, and 2D NMR (COSY, HSQC, and HMBC) experiments. The absolute configuration of compound 1 was determined using the CD spectrum and experiment data. From the present investigation, all these compounds were isolated for the first time from S. ilicifolius. It is interesting that phenylpropanoid glycoside and aromatic glycosides are reported for the first time in the genus Streblus. The chemotaxonomic significance of these compounds was summarized. Image 1 • A new phenylpropanoid glycoside was isolated from Streblus ilicifolius (Vidal) Corner. • All the compounds were isolated from S. ilicifolius for the first time. • New classes of chemical compounds were isolated from the genus Streblus for the first time. • The chemotaxonomic significance of the isolates was discussed in this study. [ABSTRACT FROM AUTHOR] more...
- Published
- 2019
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