1. Hyper-thermophilic anaerobic pretreatment enhances the removal of transferable oxazolidinone and phenicol cross-resistance gene optrA in enterococci.
- Author
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Tian, Tiantian, Yang, Xiaoxiao, Liu, Shihai, Han, Ziming, Qiao, Wei, Li, Jiuyi, Yang, Min, and Zhang, Yu
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ENTEROCOCCUS , *ANIMAL waste , *ENTEROCOCCUS faecalis , *DRUG resistance in bacteria , *POULTRY industry , *ANAEROBIC digestion - Abstract
[Display omitted] • Enterococci with optrA increased in mesophilic and thermophilic anaerobic digestion. • IS 1216E in plasmid plays key role in horizontal transfer of optrA. • Chromosomal optrA linked to Tn 554 spread vertically in dominate E. faecalis ST631. • Hyper-thermophilic anaerobic pretreatment reduced plasmid-borne optrA in enterococci. The extensive use of florfenicol in poultry industry results in the emergence of optrA gene, which also confers resistance to clinically important antibiotic linezolid. This study investigated the occurrence, genetic environments, and removal of optrA in enterococci in mesophilic (37 °C) and thermophilic (55 °C) anaerobic digestion systems, and a hyper-thermophilic (70 °C) anaerobic pretreatment system for chicken waste. A total of 331 enterococci were isolated and analyzed for antibiotic resistance against linezolid and florfenicol. The optrA gene was frequently detected in enterococci from chicken waste (42.7%) and effluents from mesophilic (72%) and thermophilic (56.8%) reactors, but rarely detected in the hyper-thermophilic (5.8%) effluent. Whole-genome sequencing revealed that optrA -carrying Enterococcus faecalis sequence type (ST) 368 and ST631 were the dominant clones in chicken waste, and they remained dominant in mesophilic and thermophilic effluents, respectively. The plasmid-borne IS 1216E - fexA - optrA - erm (A)-IS 1216E was the core genetic element for optrA in ST368, whereas chromosomal Tn 554 - fexA - optrA was the key one in ST631. IS 1216E might play a key role in horizontal transfer of optrA due to its presence in different clones. Hyper-thermophilic pretreatment removed enterococci with plasmid-borne IS 1216E - fexA - optrA - erm (A)-IS 1216E. A hyper-thermophilic pretreatment is recommended for chicken waste to mitigate dissemination of optrA from animal waste to the environment. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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