1. Neutrophil Elastase in the capacity of the “H2A-specific protease”.
- Author
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Dhaenens, M., Glibert, P., Lambrecht, S., Vossaert, L., Van Steendam, K., Elewaut, D., and Deforce, D.
- Subjects
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LEUCOCYTE elastase , *PROTEOLYTIC enzymes , *HISTONES , *CHROMATIN , *MONOCYTES , *MASS spectrometry , *EMBRYONIC stem cells - Abstract
Highlights: [•] The H2A specific protease (H2Asp) clips histone H2A at V114 but was never identified since its discovery over 35 years ago. [•] We developed an AQUA approach for high throughput quantitation of V114 clipping. [•] We show that H2Asp actually is Neutrophil Elastase (NE). [•] NE associates strongly with nucleosomes in differential gradient experiments. [•] We emphasize the potential implications of H2AV114 clipping in vitro and in vivo. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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