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We have developed a new ratiometric fluorescence probe that was constructed by dansyl and rhodamine mimic and based on Förster resonance energy transfer (FRET) platform. The probe could fast monitor trace water in organic solvents with high sensitivity and reversibility. To the best of our knowledge, this is a first ratiometric fluorescence probe based on organic small molecule for trace water in organic solvents. Moreover, test strips were made and used to detect water in organic solvents easily. In addition, Gibbs free energy in different solvents was calculated. Image 1 • A ratiometric fluorescence probe (DRY) based on FRET mechanism is designed. • DRY has a high selectivity, sensitivity for trace water in organic solvents. • DRY showed satisfactory analytical performance in terms of reversibility. • The effect of water on DRY was explained by theoretical calculation method. [ABSTRACT FROM AUTHOR]

Two ratiometric fluorescence probes, RMC and RMD, were synthesized for detecting HSO 3 − in test tube and living cells. Probe RMC based on FRET mechanism exhibited excellent ratiometric signals, high sensitivity (1.08 nM), outstanding selectivity, fast response (within 22 s) to HSO 3 − in physiological pH. The satisfactory performance of RMC in cell imaging suggested that it could be applied to monitor endogenous and intracellular bisulfite with minimal cytotoxicity and excellent photostability. More importantly, probe RMC realized targeting for lipid droplets. In practice, probe RMC could be successfully applied to detect HSO 3 − in food sugar. Besides, cheap test strips were made and used handily for rapid and qualitative detection of HSO 3 − in water by naked eye as well. Therefore, probe RMC could be a potential biological tool to explore the roles of HSO 3 − under different physiological and pathological conditions. [Display omitted] • Two ratiometric fluorescence probe RMC and RMD based on FRET for bisulfite were developed. • Probe RMC could reveal a response in 22 s toward HSO 3 − with high selectivity and sensitivity. • Probe RMC could target lipid droplets in living cells. • Probe RMC could detect HSO 3 − in sugar solution. • The test strips could be made handily for rapid and qualitative detection. [ABSTRACT FROM AUTHOR]