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Your search keyword '"Xin, Dong"' showing total 198 results
Start Over Author "Xin, Dong" Publisher elsevier b.v.
198 results on '"Xin, Dong"'

38. Isolation, antibacterial characterization, and alternating tangential flow–based preparation of viable cells of Lacticaseibacillus paracasei XLK 401: Potential application in milk preservation.

Author

Xin, Wei-Gang, Li, Xin-Dong, Zhou, Huan-Yu, Li, Xin, Liu, Wei-Xin, Lin, Lian-Bing, and Wang, Feng

Subjects
*ESCHERICHIA coli O157:H7, *LIQUID chromatography-mass spectrometry, *ESCHERICHIA coli, *GRAM-negative bacteria, *SHIGELLA flexneri, *FLUID intelligence, *CHEMOTAXONOMY, *SOCIAL isolation
Abstract

It is desirable to obtain high levels of viable Lacticaseibacillus paracasei , a widely used food probiotic whose antibacterial activity and potential application in milk remain largely uninvestigated. Here, we isolated and purified the L. paracasei strain XLK 401 from food-grade blueberry ferments and found that it exhibited strong antibacterial activity against both gram-positive and gram-negative foodborne pathogens, including Staphylococcus aureus , Salmonella paratyphi B, Escherichia coli O157, and Shigella flexneri. Then, we applied alternating tangential flow (ATF) technology to produce viable L. paracasei XLK 401 cells and its cell-free supernatant (CFS). Compared with the conventional fed-batch method, 22 h of ATF-based processing markedly increased the number of viable cells of L. paracasei XLK 401 to 12.14 ± 0.13 log cfu/mL. Additionally, the CFS exhibited good thermal stability and pH tolerance, inhibiting biofilm formation in the abovementioned foodborne pathogens. According to liquid chromatography-mass spectrometry analysis, organic acids were the main antibacterial components of XLK 401 CFS, accounting for its inhibition activity. Moreover, the CFS of L. paracasei XLK 401 effectively inhibited the growth of multidrug-resistant gram-positive Staph. aureus and gram-negative E. coli O157 pathogens in milk, and caused a reduction in the pathogenic cell counts by 6 to 7 log cfu/mL compared with untreated control, thus considerably maintaining the safety of milk samples. For the first time to our knowledge, ATF-based technology was employed to obtain viable L. paracasei on a large scale, and its CFS could serve as a broad-spectrum biopreservative for potential application against foodborne pathogens in milk products. [ABSTRACT FROM AUTHOR]

Published
2024
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40. Functional insulin aspart/insulin degludec-based microneedles for promoting postprandial glycemic control.

Author

Chen, Bo Zhi, Li, Wen Xuan, Feng, Yun Hao, Zhang, Xiao Peng, Jiao, Jie, Li, Zhuo Lin, Nosrati-Siahmazgi, Vahideh, Shahbazi, Mohammad-Ali, and Guo, Xin Dong

Subjects
INSULIN aspart, INSULIN, GLYCEMIC control, MOLECULAR dynamics, DRUG stability, INSULIN therapy, PATIENT compliance, BONE conduction, HYPERGLYCEMIA
Abstract

Insulin aspart (IAsp) and insulin degludec (IDeg), as the third generation of insulin, have a faster onset time or a more durable action period, which may simulate the secretion of insulin under physiological conditions. Microneedles (MNs) are transdermal delivery devices that may allow diabetic patients to easily deploy transdermal insulin therapy while considerably reducing injection pain. In this study, we investigated the combination of dissolving MNs with IAsp or IDeg therapy as an alternative to daily multiple insulin injections, aiming to improve glycemic control and patient compliance. Mechanical properties of the MNs, structural stability of insulin encapsulated in the MNs, and transdermal application characteristics were studied to assess the practicality of insulin-loaded MNs for diabetes therapy. In vivo experiments conducted on diabetic rats demonstrated that the IAsp- and IDeg-loaded MNs have comparable blood glucose control abilities to that of subcutaneous injections. In addition, the therapeutic properties of insulin-loaded MNs under diverse dietary conditions and application strategies were further investigated to provide new information to support future clinical trials. Taken together, the proposed MNs have the potential to improve balances between glycemic control, hypoglycemia risk, and convenience, providing patients with simpler regimens. 1. The fabricated functional insulin-loaded dissolving microneedles closely matched the glucose rise that occurs in response to meals, demonstrating promising alternatives for multiple daily insulin injections. 2. The hypoglycemic properties of insulin microneedles were investigated under diverse dietary conditions and application strategies, yielding new information to support future clinical trials. 3. Molecular dynamics simulations were utilized to study the interactions between the insulin and microneedle matrix materials, providing a strategy for theoretically understanding drug stability as well as the release mechanism of drug-loaded microneedles. [Display omitted] [ABSTRACT FROM AUTHOR]

Published
2023
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