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• The fluorescence model has a ratiometric fluorescence signal to improve accuracy. • The product DAP has a yellow color can be directly observed by the naked eye. • This dual-model immunoassay shows high sensitivity and selectivity for AFB1 detection. In this work, a dual-model immunoassay for detecting Aflatoxin B1 (AFB1) was developed based on 2,3-diaminophenazine (DAP) and carbon dots (CDs). Under the catalysis of horseradish peroxidase (HRP), the o -phthalylenediamine (OPD) was oxidized to DAP which had a yellow color and intense fluorescence. The color changes form colorless to yellow was used to design absorbance model immunoassay. Meanwhile, the absorption spectrum of DAP overlapped with the emission spectrum of CDs which caused the fluorescence of CDs to be quenched. The fluorescence changes of DAP and CDs were used to develop ratiometric fluorescence immunoassay. The dual-model immunoassay showed excellent sensitivity with the limits of detection (LODs) of 0.013 ng/mL for fluorescence mode and 0.062 ng/mL for absorbance mode. Meanwhile, both models exhibited great selectivity for AFB1. Additionally, the recovery rates suggested the proposed dual-model immunoassay had great potential in actual samples detection. [ABSTRACT FROM AUTHOR]

This work reports the use of monolayer colloidal crystal of polystyrene spheres as a template combined with electroless deposition to fabricate Ag through-sphere segment void (SSV) arrays. Experimental and theoretical (finite-difference time-domain) results reveal that the structured Ag through-SSV arrays create extraordinary optical transmission (EOT) and largely enhanced localized fields, thereby enabling EOT-based plasmonic sensing with a sensitivity of 295.38 nm/RIU (RIU = refractive index unit) and reproducible enhanced Raman signal with an enhancement factor of about 10 4 , respectively. These results suggest a low cost, feasible way to integrate plasmonic sensing and molecule-specified Raman detection on a single biochip. [ABSTRACT FROM AUTHOR]

To comprehensively assess the association of husband smoking with wives' thyrotropin abnormality. This population-based retrospective cohort study included 2 406 090 Chinese reproductive-aged women who had participated twice in the National Free Pre-pregnancy Checkups Project between 2010 and 2020. Multivariate-adjusted odds ratios and 95% confidence intervals for subnormal and supranormal thyrotropin were estimated according to the husband's smoking status. Husband smoking at the first visit was associated with a 17% (15%–20%) and 26% (24%–28%) increased odds of subnormal thyrotropin and supranormal thyrotropin respectively compared to participants in neither-smoker group. In non-smoking participants with normal thyrotropin levels at the first visit, the corresponding increased risk of subnormal thyrotropin and supranormal thyrotropin at the second visit were 15% (12%–18%) and 19% (16%–21%) in contrast to participants without husband-smoking exposure. In non-smoking participants with abnormal thyrotropin levels at their first visit, husband smoking cessation was associated with 27% (17%–35%) and 36% (31%–40%) reduced odds of subnormal thyrotropin and supranormal thyrotropin at the second visit compared with the participants whose husband still smoking at the second visit. Husband smoking was associated with wives' subnormal thyrotropin and supranormal thyrotropin, and cessation of husband smoking could reduce the odds of thyrotropin abnormality. Couple-focused smoking intervention should be developed to reduce the burden of asymptomatic thyroid disease in females. • 1. Husband smoking was associated with both supranormal thyrotropin and subnormal thyrotropin. • 2. Husband smoking cessation decrease the odds of thyrotropin abnormality. • 3. We call couple-focused smoking cessation to reduce subclinical thyroid disease. [ABSTRACT FROM AUTHOR]

Cardiovascular diseases, including hypertension, have posed a serious threat to human health in recent decades. Family-centered health promotion and disease control for the management of hypertension is gaining attention. In this study, we assessed the association between spousal educational attainment (SEA) and hypertension prevalence, awareness, and control, intending to provide new directions for family health care. A total of 71 211 191 reproductive-aged participants from the National Free NFPCP during 2013–2019 were included in the current study. Inverse probability weighting (IPW) via propensity models were used to adjust for the imbalance by SEA. Both multivariable-adjusted ORs and inverse-probability-weighted ORs were used to assess the association between SEA and the prevalence, awareness, and control of hypertension. ORs of prevalence, awareness, and control of hypertension with SEA stratified by sex, age, and residency type were also reported. Compared with participants with SEA of compulsory education, the inverse-probability-weighted ORs for hypertension were 0.97 (0.96–0.97), 0.99 (0.98–1.00), and 0.91 (0.88–0.93) for participants with SEA of senior high, college, and postgraduate, respectively. The corresponding ORs for hypertension awareness were 1.12 (1.10–1.13), 1.15 (1.13–1.16), and 1.38 (1.34–1.41). The increment of hypertension control associated with SEA was only identified in urban areas. Modification analyses revealed that urban participants were observed to have more healthy benefits associated with SEA; additional decreased prevalent hypertension and increased hypertension awareness associated with SEA were observed in wives and husbands respectively. Thus, SEA was associated with decreased prevalent hypertension and increased awareness and control of hypertension. Our findings call for increased participation of spouses in family-centered healthcare, with consideration of modified effects by gender, age, and residency type, to improve chronic disease prevention and control including hypertension. • Educated spouse improves prevalence, awareness, and control of hypertension. • Educated husband reduces more wife's hypertension prevalence. • Husband has more increased hypertension awareness associated with educated wife. • Urban people have more health benefits associated with higher spousal education. • We call for increased participation of spouses to prevent and control hypertension. [ABSTRACT FROM AUTHOR]