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[Display omitted] Ubiquitin-fold modifier 1 (UFM1) is a ubiquitin-like protein, which is involved in many critical cellular processes. In this work, we report an efficient intein-based strategy to semi-synthesize UFM1-PA and UFM1-AMC through a hydrozinolysis-aminolysis reaction. A prominent advantage of this method is that UFM1 derivatives can be readily prepared from recombinant expressed materials in ca. 2 weeks. We anticipate that these UFM1 derivatives hold promise for UFM1-related biochemical studies. [ABSTRACT FROM AUTHOR]