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• A mitochondria-targetable "naked-eye" fluorescent probe RosPO(OH) 2 was obtained. • The first dual-function probe for simultaneous determination of HOCl and pH is reported. • Probe have rapid response, low detection limit and a wide range of pH application. • The fluorescent imaging demonstrated its value of practical application. A series of rosamine dyes were designed and synthesized through one-step reaction. Among them, bifunctional fluorescent probe RosPO(OH) 2 showed excellent water solubility and large molar extinction coefficient. RosPO(OH) 2 underwent a two-step ionization process as the pH value of the HEPES buffer increased, accompanied a blue shift of UV absorption and an enhancement of fluorescence intensity. This means it can be used as a pH sensor. After the addition of HOCl, the fluorescence of RosPO(OH) 2 was quenched and the color of the solution changed from pink to colorless which permitted "naked eye" detection. RosPO(OH) 2 could be used as a sensor for detecting HOCl with high selectivity, rapid response, low detection limit (52 nM) and a wide range of pH application (pH 1–10). Colocalization results showed that RosPO(OH) 2 could specifically stain in mitochondria. Due to the low toxicity and the excellent water solubility of RosPO(OH) 2 , it had been successfully applied to image pH and HOCl in living cells. [ABSTRACT FROM AUTHOR]

Graphical abstract Highlights • A new N-oxide based and turn on fluorescent probe for Fe2+ was developed. • The probe possessed a high selectivity for Fe2+ among various biologically relevant species and fast rapid response. • Monitoring Fe2+ with TPM fluorescence imaging for the first time. Abstract Iron is an essential element for human body, involved in important biochemical processes in the body, especially in biological oxidation. Misregulation of iron levels in the cell is concerned with serious diseases. Here, a novel two photon and turn on NIR-fluorescent probe based ICT for detection of Fe2+ is first reported. The probe exhibited brilliant selectivity and excellent sensitivity (DL = 4.5 μM), rapid response (15 min) and "naked-eye colorimetric sensor" for monitoring of Fe2+. More importantly, the probe was successfully applied to real-time monitor Fe2+ in living cell through two-photon microscopy fluorescence imaging with excitation 680 nm. [ABSTRACT FROM AUTHOR]

A ER-targetable probe R1 for HOCl was synthesized. R1 shows excellent selectivity and high sensitivity, excellent anti-interference, and can quickly reach the response platform. • A new ER-targetable ICT-based fluorescent probe R1 for HClO was developed. • Probe have rapid response (30 s), big Stock's shift and a wide range of pH(7–13). • The imaging in EC1 cells demonstrated its value of practical application. The endoplasmic reticulum is the largest organelle in the cell. Studies have shown that the concentration of HClO is related to many diseases. A new type of probe R1 has positioned to the endoplasmic reticulum. The probe shows good positioning effect, excellent selectivity and high sensitivity, excellent anti-interference, and can quickly reach the response platform. The obvious color change can be used for naked eye observation. Due to its low cytotoxicity, probe R1 was successfully used for EC1 cells imaging under a laser scanning confocal microscope. [ABSTRACT FROM AUTHOR]