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Airborne bacteria and particulate matter (PM) emitted from animal houses may harm the environment and threaten on-farm and off-farm bio-security. Airborne transmission of PM and bacteria occurs more easily between double-tunnel ventilation layer hen houses with side-to-side arrangement due to the layout of inlet and outlet, and the accumulation of airborne contaminants in the intermediate region. This study practically applied shading nets for covering exhaust area and water disinfection for evaporative cooling system to prevent airborne transmission of PM and microbial aerosols between layer hen houses. Differences of PM and airborne culturable bacteria (CB) concentrations in control group and experimental group were used to evaluate the contaminants blocking effect of the treatment. PM and airborne microbial concentrations between houses were significantly higher than the upwind. Shading nets used as a barrier of PM and airborne bacteria had no significant effect on the reduction of inlet PM and airborne CB concentration of layer hen houses. The airborne CB concentration after evaporative cooling pads with water could be several times higher than that before. However, airborne CB concentration after the evaporative cooling pads with water disinfection was much lower than that before. Results indicated that shading net as a barrier of PM and airborne bacteria could hardly bring down contaminants concentrations near the inlet. Water disinfection for evaporative cooling system could largely reduce the inlet airborne CB concentration. Inlet air purification of animal houses is an effective way to prevent airborne microorganisms transmission. [ABSTRACT FROM AUTHOR]