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System for Applied Behavioral Analysis (SYSABA) is a freeware web-based software developed in Poland that aids therapists who work with developmentally disordered children. A pioneer in Poland application is adapted to Applied Behavior Analysis (ABA) methodology and is compliant with the Polish Regulation of the Ministry of Education. A part of SYSABA is a module that helps compose a compliant with Polish regulations and ABA methodology, the Individual Education-and-Therapeutic Program (pol. IPET). This development process is guided by an expert system, including specialists' knowledge of the subject. The IPET composition is demanding and time-consuming, and the decisions made by the therapist will influence the treatment results. Because there are many paths to the final curriculum, the decision-making module in SYSABA was designed to improve and standardize the process. The paper provides details of the IPETs expert module design in SYSABA. The expert knowledge of long-term supervisors was expressed in a set of regulations and constraints supplying a rule-based expert system. The unstructured expertise was translated into a logical SYSABA two-level framework. Thirty-six rules guiding skill and behavior curricula were created. The effectiveness of the expert system was confirmed by a survey after one year of SYSABA usage. [ABSTRACT FROM AUTHOR]

• GABRG3 gene rs140679 polymorphism is important risk factor in the pathophysiology of ASD. • There is role of the HTR2A gene rs6313 variant C allele in the pathophysiology of ASD. • GABRG3 gene rs140679 polymorphism was researched for the first time in a Turkish population. Autism spectrum disorder (ASD) is a neurodevelopmental and neurobehavioral disorder characterized by impaired social communication, repetitive and restricted patterns of behavior, activity, or interest, and altered emotional processing. Reported prevalence is 4 times higher in men and it has increased in recent years. Immunological, environmental, epigenetic, and genetic factors play a role in the pathophysiology of autism. Many neurochemical pathways and neuroanatomical events are effective in determining the disease. It is still unclear how the main symptoms of autism occur because of this complex and heterogeneous situation. In this study, we focused on gamma amino butyric acid (GABA) and serotonin, which are thought to contribute to the etiology of autism; it is aimed to elucidate the mechanism of the disease by investigating variant changes in the GABA receptor subunit genes GABRB3, GABRG3 and the HTR2A gene, which encodes one of the serotonin receptors. 200 patients with ASD between the ages of 3–9 and 100 healthy volunteers were included in the study. Genomic DNA isolation was performed from peripheral blood samples taken from volunteers. Genotyping was performed using the RFLP method with PCR specific for specific variants. Data were analyzed with SPSS v25.0 program. According to the data obtained in our study; In terms of HTR2A (rs6313 T102C) genotypes, the homozygous C genotype carrying frequency in the patient group and the homozygous T genotype carrying frequency in the GABRG3 (rs140679 C/T) genotypes were found to be significantly higher in the patient group compared to the control group (*p: 0.0001, p: 0.0001). It was determined that the frequency of individuals with homozygous genotype was significantly higher in the patient group compared to the control group and having homozygous genotypes increased the disease risk approximately 1.8 times. In terms of GABRB3 (rs2081648 T/C) genotypes, it was determined that there was no statistically significant difference in the frequency of carrying homozygous C genotype in the patient group compared to the control group (p: 0.36). According to the results of our study, we think that the HTR2A (rs6313 T102C) polymorphism is effective in modulating the empathic and autistic characteristics of individuals, and that the HTR2A (rs6313 T102C) polymorphism is more distributed in the post-synaptic membranes in individuals with a higher number of C alleles. We believe that this situation can be attributed to the spontaneous stimulatory distribution of the HTR2A gene in the postsynaptic membranes because of T102C transformation. In genetically based autism cases, carrying the point mutation in the rs6313 variant of the HTR2A gene and the C allele and the point mutation in the rs140679 variant of the GABRG3 gene and accordingly carrying the T allele provide a predisposition to the disease. [ABSTRACT FROM AUTHOR]