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[Display omitted] Using bio-Strips and cytotoxicity potentials, the least and most harmful substances involved in the synthesis of 4-methoxy-4 ' -nitro-1,1 ' -biphenyl from 4-methoxyphenyl- boronic acid and aryl halide were identified. A total of 36 synthetic routes were analyzed. [ABSTRACT FROM AUTHOR]

Immunoregulation mediated bone tissue engineering (BTE) has demonstrated huge potential in promoting repair of critical-size bone defects (CSBDs). The trade-off between stable immunoregulation function and extended immunoregulation period has posed a great challenge to this strategy. Here, we reported a 3D porous biodegradable Poly(HEMA- co -3APBA)/LUT scaffold, in which reversible boronic acid ester bond was formed between the 3APBA moiety and the catechol moiety of luteolin (LUT). The boronic acid ester bond not only protected the bioactivity of LUT but also extended the release period of LUT. The rationale behind the phenomenon of sustained LUT release was explained using a classical transition state theory. In vitro/in vivo assays proved the immunoregulation function of the scaffold in inducing M 2 polarization of both M 0 and M 1 M φ. The crosstalk between the scaffold treated Raw 264.7 and BMSCs were also investigated through the in vitro co-culture assay. The results demonstrated that the scaffold could induce immunoregulation mediated osteogenic differentiation of BMSCs. In addition, CSBDs model of SD rats was also applied, and the corresponding data proved that the scaffold could accelerate new bone formation, therefore promoting repair of CSBDs. The as-prepared scaffold might be a promising candidate for repair of CSBDs in the future. [Display omitted] • Reversible bond mediated bioactivity protection and sustained release of luteolin. • Immunoregulation based on inducing in situ M2 polarization of macrophages. • Immunoregulation mediated repair of critical size bone defects. [ABSTRACT FROM AUTHOR]

Dendritic cells (DCs), the primary antigen-presenting cells in the immune system, play a critical role in regulating tumor immune responses. However, the tumor immunosuppressive microenvironment severely impedes the process of antigen-presenting and DC maturation, thereby limiting the efficacy of cancer immunotherapy. In this work, a pH-responsive polymer nanocarrier (PAG) modified with aminoguanidine (AG) was constructed for the efficient delivery of bortezomib (BTZ) through bidentate hydrogen bonds and electrostatic adsorption formed between guanidine groups of PAG and boronic acid groups of BTZ. The obtained PAG/BTZ nanoparticles exhibited pH-responsive release of BTZ and AG in the acidic tumor microenvironment. On the one hand, BTZ induced potent immune activation by eliciting immunogenic cell death (ICD) and releasing damage-associated molecular patterns. On the other hand, the cationic AG significantly promoted antigen uptake by DCs and activated DC maturation. As a result, PAG/BTZ significantly stimulated tumoral infiltration of cytotoxic T lymphocytes (CTLs) and triggered robust antitumor immune responses. Thus, it showed potent antitumor efficacy when synergizing with an immune checkpoint-blocking antibody. A pH-responsive aminoguanidine (AG)-modified nanoparticle (PAG/BTZ) for tumoral delivery of bortezomib (BTZ) was fabricated successfully to improve the chemo-immunotherapy efficacy by eliciting immunogenic cell death (ICD), promoting dendritic cells (DCs) maturation, and synergizing with an immune checkpoint-blocking antibody (α-PDL1). [Display omitted] • The PAG/BTZ nanoparticles induced strong tumor immunogenic cell death to increase the immunogenicity. • The PAG/BTZ nanoparticles improved chemo-immunotherapy efficacy by activating DCs and promoting their maturation. • The PAG/BTZ showed potent antitumor efficacy when synergizing with an immune checkpoint-blocking antibody (αPD-L1). [ABSTRACT FROM AUTHOR]

[Display omitted] • PLA-based nanofibrous wound dressings were developed with organomodified nanoclay (OMMT) and various boron compounds. • Unlike the literature, different boron compounds, boron nitride, zinc borate and phenyl boronic acid were used. • The addition of OMMT and B compounds enhanced the thermal stability, antibacterial effect, and hydrolytic degradation rate of the PLA. • ATR-FTIR analysis supported the intercalated composite formation. • Matrices including zinc borate and phenyl boronic acid demonstrated both bacteriostatic and bactericidal effects. In this study, nanofibrous polymeric matrices were successfully developed with nanoclay, montmorillonite (MMT) and various boron (B) compounds, which were known to have positive effects on the wound healing with elevated antibacterial properties. For this purpose, MMT was modified with quaternary ammonium salt, trimethyl octadecyl ammonium bromide (TMOD), and boron compounds, boron nitride (BN), zinc borate (ZB), or phenylboronic acid (PBA) were adsorbed on organomodified MMT (OMMT). Then, poly (lactic acid) (PLA) based nanofibrous PLA-OMMT/B composites were fabricated via electrospinning. Modification of MMT nanoparticles with TMOD occurred through ion-exchange reaction and led to better homogenous fibrous structures which exhibited dramatic inhibition for gram-positive bacteria. Moreover, composites with ZB and PBA demonstrated both bacteriostatic and bactericidal effects for gram-positive and gram-negative bacteria. The chemical structures of the matrices were evaluated through ATR-FTIR and supported the intercalated composite formation. The thermal and mechanical stabilities of PLA matrices were also enhanced after OMMT and B incorporation. The lowest breaking strain value was recorded for PLA-OMMT/PBA composite compared to other B composites. The 100% and 50% extracts of the PLA-OMMT matrices showed modest cytotoxic effect on the human dermal fibroblasts (NHDF) on the second day culture that probably originated from TMOD. These results demonstrated that PLA-OMMT/B matrices, especially PBA including matrices, can be used as replaceable wound dressings that have limited interaction with cells but exhibit antibacterial activity and support the early stages of wound healing both morphologically and chemically. [ABSTRACT FROM AUTHOR]

Rational design of efficient cytosolic protein delivery carriers holds enormous promise for biotherapeutics development. Several delivery systems have been developed during the past decades, while tailoring the balance between extracellular protein binding and intracellular cargo release is still challenging. In this study, we synthesized a series of oxygen-sensitive reactive polymers, rich in boron, by radical polymerization and post-modification for cytosolic protein delivery in vitro and in vivo. The introduction of boronate building blocks into the polymer scaffold significantly enhanced its protein binding affinity, and the polymer/protein complexes with high stability were obtained by tailoring the molecular ratios between the boronate ligands and the amine groups. The lead material screened from the polymer library exhibited efficient protein delivery efficacy that can release cargo proteins in cytosol in a reactive oxygen species responsive manner, which enables intracellular delivery of proteins with maintained bioactivity. In addition, the polymer-based nanoformulations efficiently delivered saporin, a toxin protein, into osteosarcoma cells and tumor tissues, and exhibited high therapeutic efficacy in an osteosarcoma mouse model. The synthesized polymer in this study can be developed as a promising nanocarrier for cytosolic delivery of protein therapeutics to treat a variety of diseases. ROS-responsive boronic acid-rich polymer achieves efficient intracellular protein delivery in vitro and in vivo. [Display omitted] • A boronate-rich polymer exhibits efficient protein binding and ROS-responsive protein release. • Protein delivery efficacy can be tailored by the boronate ratios and the amine structures. • Efficient in vivo cancer therapy was achieved via tumor targeted protein delivery. [ABSTRACT FROM AUTHOR]

An enzyme-based fluorescent sensor was developed by adapting carbon dots derived from coffee waste to detect inhibitory neurotransmitters such as γ-aminobutyric acid (GABA). In this study, CDs were synthesized from biomass-derived coffee waste by the facile hydrothermal reaction (i.e., C-CDs). A C-CDs-based enzyme-based sensor was then developed using 3-aminophenyl boronic acid (APBA) and nicotinamide adenine dinucleotide phosphate (NADP+) cofactors (i.e., C-CANs). As a result of the addition of the GABase enzyme, the resulting blue-fluorescent C-CANs were effective in detecting GABA. This sensor is capable of sensing GABA in the range of 0–20 μM with a detection limit of 95.09 nM. A distinguished fluorescence quenching was observed in human neuroblastoma (SH-SY5Y) cells where probe (C-CANs-GABase) was able to detect GABA intracellularly. Zebrafish larvae were used to study the sensing potentials of the developed probe against GABA at different concentrations (10 and 20 μM). A validation study was conducted on real samples such as human serum, which showed high recovery values between 97 and 105.6%. [Display omitted] [ABSTRACT FROM AUTHOR]

Hydrogen peroxide, organic peroxides and hydroperoxides exhibit high reactivity and play a pivotal role in atmospheric chemistry. These compounds are formed during the oxidation of volatile organic compounds in both gaseous and aqueous phases, particularly under low NOx conditions. Their significant contribution to the mass of secondary organic aerosols (SOA) is well-documented, and they are believed to have significant health implications. Several spectrophotometric methods have been employed to measure SOA-bound peroxides in laboratory samples, but systematic comparisons are lacking. In this study, we have assessed the advantages and limitations of these methods, including the traditional and microwave-assisted iodometric methods, the 4-nitrophenyl boronic acid assay (NPBA), and the Fenton reaction-assisted ferrous-xylenol (FOX2) assay. Besides, a comprehensive evaluation of these methodologies was conducted for the first time across a substantial cohort of commercial peroxides and hydroperoxides, employing diverse solvents (namely, water, 1-propanol, acetonitrile, methanol and chloroform) to provide broader insights compared to previous work. Ultimately, the four methods were applied and compared for peroxide determination in laboratory-generated SOA resulting from gas-phase ozonolysis of α-pinene. This study opens new opportunities for future mechanistic investigation into SOA formation and reactivity. [Display omitted] • Evaluation of four assays for detecting peroxides in SOA. • Detailed comparison of detection limits. • Methods applicable to water and 1-propanol extracted samples. • Enhanced peroxide and hydroperoxide detection and quantification. • Improved understanding of SOA formation achievable combining different methods. [ABSTRACT FROM AUTHOR]

We developed a highly versatile transition metal-free Suzuki type cross-coupling protocol. Alkenyl/aryl boronic acids could couple smoothly with propargylic mesylates Csp3 electrophiles in the presence of mild bases. The counterion in the base plays a crucial role for the reactivity. Our metal-free condition is orthogonal towards the classic transition metal catalysed Suzuki reactions. Stereospecific nucleophilic nubstitution of alkenyl boronic acids with bulk sp3-Carbon electrophiles. [Display omitted] • Bulky sp3-carbon electrophiles could couple with alkenyl boronic acids under mild conditions. • Counterion (K+, Na+) assisted nucleophilic substitution is different from conventional nucleophilic substitution. • This metal-free coupling protocol is stereo-specific. [ABSTRACT FROM AUTHOR]

The β -arylated meso -tetra(p -tolyl) triphyrins[2.1.1] containing two, three, and four p -fluorophenyl groups were synthesized by reacting appropriate β -bromo triphyrin[2.1.1] with p -fluorophenyl boronic acid in toluene/THF/H 2 O (1:1:1) at 80 οC for 8 h–12 h and their properties were compared with our previously reported β -arylated triphyrins[2.1.1] containing one and six p -fluorophenyl groups. The increase of the number of p -fluorophenyl groups at the β -pyrrole carbons induces steric hindrance at the periphery of triphyrin[2.1.1] that results in the alteration of electronic properties due to distortion in the macrocycle which is reflected in their structure, spectral and redox properties. The X-ray structure analysis revealed that β -di(p -fluorophenyl) meso -tetra(p -tolyl) triphyrin[2.1.1] was almost planar like meso -tetra(phenyl) triphyrin[2.1.1] whereas the β -hexa(p -fluorophenyl) meso -tetra(p -tolyl) triphyrin[2.1.1] was significantly distorted. The absorption spectra of β -arylated triphyrins[2.1.1] showed bathochromically shifted Soret and Q-bands with reduction in absorption coefficients and increase in the bandwidth compared to the β -unsubstituted meso -tetra(p -tolyl) triphyrin[2.1.1] and the maximum effects were noted for β -hexa(p -fluorophenyl) meso -tetra(p -tolyl) triphyrin[2.1.1]. The β -arylated meso -tetra(p -tolyl) triphyrins[2.1.1] are stable under electrochemical conditions and DFT/TD-DFT studies are in agreement with the experimental observations. [Display omitted] • Synthesized β -arylated meso -tetra(p-tolyl) triphyrins by adding p -fluorophenyl groups. • Adding p -fluorophenyl groups at β -pyrrole carbons increases steric crowding and alters properties. • β-Arylated meso-tetra(p-tolyl) triphyrins are stable under electrochemical conditions. [ABSTRACT FROM AUTHOR]

• The PdNPs-DDIL@PS catalyst was created by immobilizing PdNPs on DABCO-functionalized ionic liquid supported on Merrifield resin. • The catalyst was examined using SEM, TEM, TGA-DTA, and XPS. • It is highly effective in catalyzing the Suzuki coupling reaction of various aryl halides in 70 % aqueous ethanol at room temperature. Additionally, the protocol was applied to Heck coupling reactions in DMF at 80 °C. • For the Suzuki and Heck coupling reactions, the catalyst achieved a good TON and TOF. • The catalyst can be recycled at least four times for the Suzuki coupling reaction. A catalyst named PdNPs-DDIL@PS has been synthesized by immobilizing PdNPs on a DABCO Dicationic Ionic Liquid (DDIL) supported on Merrifield Resin (PS). The PdNPs (∼4.16 nm size) generated in situ were fully characterized using different techniques such as SEM, TEM, TGA-DTA, and XPS. It has been found that the catalyst is highly active in the Suzuki coupling reaction of various aryl bromides and aryl boronic acids in 70 % aqueous ethanol at room temperature. The catalyst produced the desired Suzuki coupling products in good to excellent yields. Additionally, the protocol was extended for the Heck coupling reactions in DMF at 80 °C with good to excellent yields. The catalyst attained a good turnover number (TON) of 160.00–189.92 and a turnover frequency (TOF) of 8.493–18.992 min−1 for the Suzuki and TON of 173.33–189.77 and TOF of 3.952–6.325 min−1 for the Heck coupling reactions. The catalyst displayed at least four times recyclability for the Suzuki coupling without a substantial decrease in product yields. Additionally, it boosts impressive environmentally-friendly credentials in Suzuki coupling reactions. [Display omitted] [ABSTRACT FROM AUTHOR]

• Boronate-based fluorescence probes (L1-L3) were developed for imaging viscosity and H 2 O 2. • N-hydroxyethyl substitution has almost no effect on the TICT property for establishing fluorescent probes. • L3 exhibited good response to viscosity and H 2 O 2 with high sensitivity and selectivity. • L3 was successfully applied to monitor viscosity and H 2 O 2 in living HepG2 cells. A complicated relationship exists between viscosity and hydrogen peroxide (H 2 O 2) in biological systems, whose changes are important indication of many diseases. To provide efficient tools for exploring this, we devise and synthesize three boronate-based fluorescence probes (L1 – L3) by incorporating different N-hydroxyethyl substituted aminobenzaldehydes with boronated pyridinium group respectively. L3 with good properties and water solubility has been chosen for further study. L3 exhibits good response to viscosity and H 2 O 2 with excellent sensitivity and selectivity. The reaction mechanism has been proposed as the restriction of twisted intramolecular charge transfer (TICT) to high viscosity resulting in strong red fluorescence, while boronic acid moiety is cleaved to release by H 2 O 2 causing green fluorescence due to intramolecular charge transfer (ICT). Moreover, L3 has been successfully applied to monitor viscosity and H 2 O 2 in living cells. This work demonstrates that N-hydroxyethyl substitution does not alter the electron donating property of the aminobenzene group, which provides an ideal example to develop promising tools for monitoring bioactive processes/species in living systems. [ABSTRACT FROM AUTHOR]

[Display omitted] • First PISA of N -phenylacrylamide and copolymerization with boronic acid derivative. • Copolymerization stabilizes boronic acid/boroxine-containing nanoparticles. • Stimuli-response is obtained from free boronic acid PISA dispersions. • Boroxine NP hydrolysis leads to sophisticated morphologies. • PISA dispersions give smaller nanoparticles with reaction of base and glucose. Boronic acid (BA) moieties confer a variety of stimuli-response properties upon polymers. PISA is the most efficient technique for the preparation of core–shell nanoparticles (NPs), however aqueous dispersion PISA of free BA-containing monomers is complicated by the formation of the boroxine anhydride at the hydrophobic core. In the present work, dispersion reversible addition-fragmentation chain transfer (RAFT) copolymerization of a free BA-containing derivative of N -phenylacrylamide yields NP morphologies which are different to those formed from the equivalent PISA homopolymerization without BA. Depending on conditions, PISA results in spheres, rods, and worms with colloidal stability improved by using a higher fraction of the non-stimuli responsive monomer to give large compound micelles. Post-polymerization stimuli-response from the resultant PISA dispersion through hydrolysis of boroxine-rich copolymer NPs by dilution with the aqueous dispersion solvent or with water yields micron-sized worms, lamellae, and vesicles. Self-assembly to higher order morphologies is driven by the extent of hydrolysis. The resultant free BA-containing NPs becoming smaller and spherical upon basification and glucose addition. [ABSTRACT FROM AUTHOR]

Simply prepared emulsion imprinted microspheres with specific recognition cavities for efficient separation and purification of boronate based metal organic frameworks (MIL-100-B). The obtained high-performance MIL-100-B (H-MIL-100-B) were further analyzed the adsorption performance of cis -diol containing molecules Naringin (NRG). [Display omitted] • A dynamic MOF-imprinted purification system based on emulsion microreactor were designed. • The purified H-MIL-100-B possessed a large adsorption amounts of 43.69μmol/g. • Purified NRG purity is 93.93% with a high biological activity. • A high score (0.75) was predicted via AGREE approaches, suggesting environmentally friendly separation process. The boronic acid suspended Cr based MIL-100 (MIL-100-B) sorbents have been widely used to separation of cis -diol containing molecules. However, it's still challenging in fabrication of high performance metal organic frameworks (MOFs) due to its irregular size and low content of functional groups. For improving identification efficiency, the emulsion microreactor were firstly designed to purify MIL-100-B prior to their use. Herein, a highly ordered MOFs-imprinted polymer microsphere was synthesized by O/W Pickering emulsion template, which can highly selective separation of MIL-100-B via the synergistic effect of boronic acid sites and imprinted cavities. The obtained purified H-MIL-100-B exhibited the uniform size and abundant boronic acid groups (B elements ratio up to 27.37 %). The emulsion imprinted microsphere (EIMs) showed the good selectivity towards MIL-100-B. Additionally, the obtained MOF-imprinted microsphere exhibited green, convenient, sustainable separation process towards the purification and recycle of MIL-100-B. The purified MOFs (H-MIL-100-B) were used to specific bind with cis -diol flavoniods naringin (NRG). Satisfactorily, H-MIL-100-B show high adsorption amount (43.69μmol/g at 308 K), fast capture kinetics (160 min), high selectivity and excellent regenerability (up to seven cycles). Remarkably, the purified NRG solutions displayed lasting antibacterial activity against Staphylococcus aureus (S. aureus) with inhibition zone of 13.47 mm. More importantly, the H-MIL-100-B could effectively promote the specific recognition ability of NRG by reducing matrix interference and improving purification efficiency (93.93 %) from complex natural extracts. A high score (0.75) was predicted via analytical GREEnness (AGREE) approaches, demonstrating environmentally friendly and greenness of separation process. The MOFs-imprinted emulsion microspheres possessed dynamic recognition properties towards target MOFs, thus opening new direction for the development of convenient and efficient microreactor for the purification of other functional MOFs. [ABSTRACT FROM AUTHOR]

Phenylboronic acid (PBA) is recognized as one of the most promising cancer cell binding modules attributed to its potential to form reversible and dynamic boronic ester covalent bonds. Exploring the advanced chemical versatility of PBA is crucial for developing new anticancer therapeutics. The presence of a specific Lewis acidic boron atom-based functional group and a Π-ring-connected ring has garnered increasing interest in the field of cancer immunotherapy. PBA-derivatized functional biomaterials can form reversible bonds with diols containing cell surface markers and proteins. This review primarily focuses on the following topics: (1) the importance and versatility of PBA, (2) different PBA derivatives with pKa values, (3) specific key features of PBA-mediated biomaterials, and (4) cell surface activity for cancer immunotherapy applications. Specific key features of PBA-mediated materials, including sensing, bioadhesion, and gelation, along with important synthesis strategies, are highlighted. The utilization of PBA-mediated biomaterials for cancer immunotherapy, especially the role of PBA-based nanoparticles and PBA-mediated cell-based therapeutics, is also discussed. Finally, a perspective on future research based on PBA-biomaterials for immunotherapy applications is presented. [Display omitted] • A compressive overview for phenylboronic acid (PBA) derivatives with pKa values. • Importance and specific key features of the PBA biomaterials are highlighted. • Elucidating the designing of PBA-biomaterials engineering toolsets. • Potential utilization of PBA-biomaterials for cancer immunotherapy. [ABSTRACT FROM AUTHOR]

[Display omitted] • Tb-MOG was synthesized by a simple solvothermal method. • Tb-MOG acts as a gel with more stable ECL signal and efficiency compared to other materials. • The boronic acid group undergoes metal transfer reaction with Hg2+ to enhance the ECL emission. • The proposed method is highly selective for electrochemiluminescence detection of Hg2+ with LOD as low as 0.53 μM. Recent advancements in electrochemiluminescence (ECL) have highlighted the potential of metal–organic gels (MOGs) as innovative luminescent materials for environmental monitoring. This study introduces a terbium-based Metal–Organic Gel (Tb-MOG) synthesized through a straightforward solvothermal method, exhibiting quantitative detection of mercury ions (Hg2+). The high mesoporosity of Tb-MOG facilitates co-reactant penetration and the gel does not come off easily when applied to the electrodes, enhancing the stability and efficiency of ECL signals. The organic ligand 5-borophenyl-1,3-dicarboxylic acid (5-bop) serves dual functions: it sensitizes Tb3+ luminescence and specifically recognizes Hg2+. Upon the interaction with Hg2+, the boronic acid group of 5-bop undergoes a metal transfer reaction, resulting in an enhanced 'antenna' effect, which enhances ECL emission. The linear range for Hg2+ of the proposed method was from 1.2 μM to 24.6 μM, with a detection of limit (LOD) of 0.53 μM. [ABSTRACT FROM AUTHOR]

With time, nickel complexes have emerged as effective alternative, as well as relatively inexpensive, catalyst for SMCCs. Nickel being a congener of palladium, is able catalyze the coupling reaction in a closely similar way as palladium. Several research groups around the globe have been successfully using nickel complexes as catalyst-precursor in SMCCs, and the present micro review article has showcased the recent developments in this area. [Display omitted] • Discussed the importance of nickel-catalyzed Suzuki-Miyaura C C cross-coupling as a pivotal tool in organic synthesis. • Covered recent advances in Ni-catalyzed SMCC using various coupling partners, viz. alkyl, heteroaryl aside from usual aryl substrates. • Sheds light on several heterogeneous nickel catalysts used in SMCCs. • Provides mechanistic insights into catalytic pathways. The palladium-catalyzed C C cross-coupling between aryl halides and aryl boronic acids was first reported by Prof. Akira Suzuki in 1979. Since then, such type of coupling reaction is known as Suzuki-Miyaura cross-coupling (SMCC) after the name of Prof. Suzuki and his associate Dr. Norio Miyaura. Gradually, complexes of other 4d and 3d metals were also found capable of catalyzing such reaction with different degrees of efficiency. With time, nickel complexes have emerged as effective alternative, as well as relatively inexpensive, catalyst for SMCCs. Nickel being a congener of palladium, is able catalyze the coupling reaction in a closely similar way as palladium. Several research groups around the globe have been successfully using nickel complexes as catalyst-precursor in SMCCs, and the present micro review article has showcased the recent developments spanning over last fourteen years in this area. [ABSTRACT FROM AUTHOR]

[Display omitted] • Acid pretreatment of biomass to afford monosaccharides, predominantly D-xylose (>90 % selectivity, and ∼ 17–18 % yield). • High purity (>98 %) recovery of D-xylose in solid dry form (∼10 wt% yield with respect to SAW) from acid hydrolysate. • A rapid (∼10 min) chemical conversion of D-xylose to xylitol using NaBH 4 /wet-SiO 2 under solvent-free conditions at RT. • D-xylose to xylitol conversion could be realized without energy expenditure in terms of temperature and pressure. Xylitol is occurring naturally as a minor constituent in many fruits and vegetables. Commercially, xylitol is produced by chemical methods from D-xylose for application as an alternative sweetener in food and pharmaceutical products. Although biomass-derived xylose can be used as a potential substrate for xylitol production, its prerequisite purification before a catalytic hydrogenation directly impacts the production cost. In addition, the isolation of xylose falls under a routine pretreatment activity during conversion of biomass to platform chemicals. The resulting hydrolysate often exhibits a low concentration of xylose and other monosaccharides. Concentrating the hydrolysate to a desired level for conversion to xylitol under chemical/biological methods increases the expenditure cost. Many times, concentration by heating may cause the degradation of contained sugars in the hydrolysate. Therefore, this study has been carried out to obtain a mixture of C5/6 sugars, predominantly the D-xylose in a solid dry form (∼10 % yield, >98 % purity) directly from acid hydrolysate of spent aromatic waste. Biomass-derived D-xylose was further transformed to xylitol (100 % conversion) by a fast (10 min), efficient, and solvent-free chemical approach using a modified sodium borohydride (NaBH 4) catalyst on a wet silica (SiO 2) support at RT. All reagents and solvents used in the process were recovered and recycled, leading to the minimal waste generation. [ABSTRACT FROM AUTHOR]

[Display omitted] • Formation of 4-nitrobenzenethiol from 4-mercaptophenyl boronic acid. • Products of 4-mercaptophenyl boronic acid deboronation monitored by SERS. • Experimental conditions affect reactions of 4-mercaptophenyl boronic acid. Plasmon-driven reactions on plasmonic nanoparticles (NPs) occur under significantly different conditions from those of classical organic synthesis and provide a promising pathway for enhancing the efficiency of various chemical processes. However, these reactions can also have undesirable effects, such as 4-mercaptophenylboronic acid (MPBA) deboronation. MPBA chemisorbs well to Ag NPs through its thiol group and can subsequently bind to diols, enabling the detection of various biological structures by surface-enhanced Raman scattering (SERS), but not upon its deboronation. To avoid this reaction, we investigated the experimental conditions of MPBA deboronation on Ag NPs by SERS. Our results showed that the level of deboronation strongly depends on both the morphology of the system and the excitation laser wavelength and power. In addition, we detected not only the expected products, namely thiophenol and biphenyl-4,4-dithiol, but also 4-nitrothiophenol (NTP). The crucial reagent for NTP formation was an oxidation product of hydroxylamine hydrochloride, the reduction agent used in Ag NP synthesis. Ultimately, this reaction was replicated by adding NaNO 2 to the system, and its progress was monitored as a function of the laser power, thereby identifying a new reaction of plasmon-driven −B(OH) 2 substitution for –NO 2. [ABSTRACT FROM AUTHOR]

Water-compatible SMIMs based on boronate affinity and DA/PEI coating for efficient enrichment, separation and detection of capecitabine. [Display omitted] • Hydrophilic SMIMs were prepared using PDA/PEI coating and boronate affinity. • Uniform boronic acid microspheres were prepared via dispersion polymerization. • The SMIMs exhibited excellent recognition performance in aqueous media. • The SPE integrates the selective enrichment, separation and detection for CAP. • The study provides a facile and effective method for bio-analysis of trace drugs. A novel water-compatible surface molecularly imprinted polymer microspheres (SMIMs) based on boronate affinity and hydrophilic coating were developed for the specific recognition and extraction of capecitabine (CAP). The boronic acid-functionalized polymer microspheres (BPMs) were first prepared as the carrier through dispersion polymerization using methyl methacrylate, ethylene glycol dimethacrylate, and 4-vinylphenylboronic acid as the polymerization monomers. Subsequently, the CAP template was immobilized onto the surface of the BPMs by virtue of boronate affinity. Finally, a thin and adherent imprinting layer was coated onto the surface of the BPMs via in-water self-polymerization of dopamine (DA) and polyethyleneimine (PEI). The resulting SMIMs exhibited strong hydrophilicity, high adsorption capacity (151.52 μmol/g) and a great imprinting factor (5.99) for CAP in aqueous media. The adsorption behavior of CAP onto the SMIMs was in good agreement with the Langmuir model and the pseudo-second-order equation. Selective adsorption indicated that the SMIMs exhibited a higher specific recognition for CAP compared to other structural analogs. In addition, the SMIMs showed excellent reproducibility and reusability, and could be utilized as an adsorbent in solid-phase extraction (SPE) for the selective pre-concentration, purification, and detection of trace amounts of CAP in spiked urine samples in combination with HPLC. The elution recovery of CAP for the various spiked levels ranged from 91.7 % to 104.9 %, with a relative standard deviation (RSD) of less than 5.8 % (n = 3). [ABSTRACT FROM AUTHOR]

Development of ultra–sensitive and selective fluorescent probes exclusively targeting CH 3 Hg+, especially against Hg2+, in real ecosystems like groundwater remains a critical yet formidable challenge. In this contribution, we present a series of fluorescent carbazole boronic acids (probes 1 – 4) designed for the highly sensitive and selective detection of Hg2+ and CH 3 Hg+ using an innovative 3–in–1 strategy. Notably, probe 4 exhibited exceptional performance in mercury detection in real groundwater, achieving a limit of detection concentration as low as 1.0 ppb for both Hg2+ and CH 3 Hg+, even in the presence of elevated levels of interfering cations or anions. Subsequently, a new fluorescent probe system was established by embedding probe 4 into cationic micelles, employing a microphase separation protocol. This resulted in exclusive selectivity towards CH 3 Hg+ over Hg2+ and other interfering ions with sensitivity as low as 10.0 ppb. This advancement not only addresses a critical gap in environmental monitoring but also demonstrates the potential for broader applications of these probes in sensitive detection methodologies. • A conceptionally new 3–in–1 strategy for crafting fluorescent probes featuring ultrahigh sensitivity and selectivity. • The fluorescent probe has been successfully applied to the detection of Hg2+ and CH 3 Hg+ in groundwater. • The microphase separation strategy significantly enhanced the probe's ability to selectively detect CH 3 Hg + over Hg2+. [ABSTRACT FROM AUTHOR]

As an endogenous gasotransmitter, CO has achieved tremendous advances in cancer treatment through selectively killing cancer cells. However, the application of CO in tumor immunotherapy has not been reported and the tumor targeting delivery is still a tremendous challenge. Herein, thermosensitive boronic acid group-containing CO prodrug was synthesized and fabricated with tannic acid (TA) and iron (Fe) to form metal-phenolic networks, and then loaded with near-infrared (NIR) photothermal agent IR820 to form FeCO-IR820@FeIIITA for combinational therapy of CO and photothermal therapy. Ferroptosis can also be enhanced due to the Fe3+ incorporation. After TA reduced Fe3+ into Fe2+, Fe2+ might lead to intracellular Fenton reaction. Furthermore, in combination with CTLA-4 blockade immunotherapy, FeCO-IR820@FeIIITA remarkably inhibited breast tumor growth, suppressed the lung metastasis and improved the antitumor immune response. To summarize, FeCO-IR820@FeIIITA provides a potential novel option for CO/photothermal/immune synergistic therapy with enhanced ferroptosis through simple compositions and facile synthesis process. [Display omitted] [ABSTRACT FROM AUTHOR]

Si undergoes significant volume change over cycles which degrades the structural integrity and stability of the electrode. This volume change is the primary barrier to the commercialization of Si anodes, and it is more pronounced for Si particle sizes over 150 nm. In this study, a crosslinked polymer binder is developed using poly(acrylic acid- co -acrylamide) (PAAM) with enhanced elasticity compared to the widely used poly(acrylic acid). PAAM is further grafted with boronic acid and dopamine, yielding PAAM-B-D, a binder with improved adhesion due to its 3D crosslinked network. Additionally, 350-nm Si is coated with cyclized polyacrylonitrile (cPAN) and heat-treated to form a conjugated structure. The cPAN-coated Si (cSi) exhibits enhanced conductivity and mechanical stiffness and is used as an active material. The developed Si anode effectively combines cPAN-coated Si with the crosslinked network formed in the PAAM-B-D polymer for enhanced adhesion. The cSi@PAAM-B-D electrode sufficiently maintains its structural integrity and mitigates the Si volume change even with the large-sized 350-nm Si. The cSi@PAAM-B-D exhibits a high initial Coulombic efficiency of 86.5 %, at a Si mass loading of 2 mg cm−2. It also shows a capacity retention of 83.6 % and a high areal capacity of 3 mAh cm−2 after 50 cycles. [Display omitted] • Si anode operation beyond critical size (350 nm Si) through dual strategy. • Coating the Si particles with a rigid polymer helped suppress volume change. • A polymer binder with improved elasticity relieved stress during volume expansion. • The cSi@PAAM-B-D shows high cycle performance and rate capability. [ABSTRACT FROM AUTHOR]

[Display omitted] • A new fluorescent probe R4B as theranostic in colorectal cancer cells was designed. • High selectivity, sensitivity and anti-interference for sialic acid. • Selective cytotoxicity against HCT 116 and HT-29 colorectal cancer cell lines. • The probe induced time-dependent mitochondrial-mediated apoptosis in HT-29 cells. • The probe detected intracellular sialic acid in HT-29 cells. A new probe, 4-(((3′,6′-bis(diethylamino)-3-oxospiro[isoindoline-1,9′-xanthen]-2-yl)imino)methyl)phenyl)boronic acid (R4B) was prepared by facile condensation of 4-formylphenylboronic acid and rhodamine B hydrazide. R4B was characterized by spectroscopic methods and single crystal X-ray diffraction. The sensor R4B solution turned pink and emitted orange fluorescence only in the presence of sialic acid but remained colorless and non-fluorescent otherwise. The sugar recognition performance was investigated via UV–vis and fluorescence spectroscopic studies. Our results revealed that R4B has good affinity and selectivity for sialic acid over common monosaccharides, with a detection limit as low as 10−7 M. Furthermore, R4B selectively inhibited growth of human colorectal adenocarcinoma HT-29 (IC 50 <20 µM) without significant cytotoxicity to normal human colon fibroblasts CCD-18Co. Treatment with R4B suppressed HT-29 colony formation via mitochondrial apoptosis in a time-dependent manner. Cellular imaging studies also revealed the ability of R4B as a fluorescence dye to detect intracellular sialic acid and showed mitochondria-tracking ability in HT-29 cells. In summary, R4B is a potential theranostic for the detection of intracellular sialic acid during the early incubation period, followed by induction of cancer apoptotic cell death at a later treatment point. [ABSTRACT FROM AUTHOR]

• Facile assembly of a small library of N-heteroarylated quercetin derivatives via Suzuki-Miyaura reaction are realized • 28 N-heteroarylated quercetin derivatives were tested against 3 types of human cancer cell lines (MGC-803, HCC827, and OVCAR-3) in vitro • quinolin-7-yl substituted compound 2m exhibited best activity (IC 50 = 3.20 µmol/L) against HCC827 cancer cell lines at µmol/L level • The preliminary structure-activity relationship of pyridyl and quinolinyl substituted quercetin was disclosed. The synthesis of a small library of N -heteroaryl substituted quercetin derivatives (28 compounds) at the C8 position applying Suzuki-Miyaura cross-coupling reaction between 8-iodoquercetin methyl ether and various pyridylboronic acids or quinolylboronic acids is reported. The obtained quercetin derivatives 2a - 2n and 3a - 3n were well characterized by the 1H NMR, 13C NMR and HRMS data. Preliminary biological evaluations showed that some of the synthesized 8-heteroarylated quercetin derivatives possessed very high anti-cancer cell proliferation activity in HCC827 cancer cell line (IC 50 < 5.0 µmol/L) in vitro. Among these synthesized compounds, quinolin-7-yl substituted compound 2m exhibited best activity in HCC827 (IC 50 = 3.20 µmol/L), while quinolin-3-yl substituted compound 2i showed best activity in MGC-803 cells (IC 50 = 14.92 µmol/L). The 3-pyridyl compound 3c with ortho -fluorine showed best activity in OVCAR-3 cells (IC 50 = 10.67 µmol/L). The disclosed structure-activity relationship provides hints that large steric hindrance at the C8 position of quercetin alter the anticancer activities and also offers new examples for new application of electronic and steric modifications as versatile tools in drug discovery. The synthesis of a small library of N -heteroaryl substituted quercetin derivatives (28 compounds) at the C8 position applying Suzuki-Miyaura cross-coupling reaction between 8-iodoquercetin methyl ether and various pyridylboronic acids or quinolylboronic acids is reported. The obtained quercetin derivatives 2a - 2n and 3a - 3n were well characterized by the 1H NMR, 13C NMR and HRMS data. Preliminary biological evaluations showed that some of the synthesized 8-heteroarylated quercetin derivatives possessed very high anti-cancer cell proliferation activity in HCC827 cancer cell line (IC 50 < 5.0 µmol/L) in vitro. Among these synthesized compounds, quinolin-7-yl substituted compound 2m exhibited best activity in HCC827 (IC 50 = 3.20 µmol/L), while quinolin-3-yl substituted compound 2i showed best activity in MGC-803 cells (IC 50 = 14.92 µmol/L). The 3-pyridyl compound 3c with ortho -fluorine showed best activity in OVCAR-3 cells (IC 50 = 10.67 µmol/L). The disclosed structure-activity relationship provides hints that large steric hindrance at the C8 position of quercetin alter the anticancer activities and also offers new examples for new application of electronic and steric modifications as versatile tools in drug discovery. [Display omitted] [ABSTRACT FROM AUTHOR]

Rapid and selective separation, enrichment and detection of trace residue all-in-one in complex samples is a major challenge. Hydrogels with molecular sieve properties can selectively separate and enrich target analytes, and the combination with high sensitivity detection of surface-enhanced Raman scattering (SERS) is expected to achieve the above all-in-one detection. Herein, the core-shell structured Au@poly(N-isopropylacrylamide)-phenylboronic acid hydrogel (Au@PNIP-VBA) with boronic acid ester groups was prepared by thermally initiated polymerization. The boronic acid ester groups in hydrogel are selectively hydrolyzed by hydrogen peroxide (H 2 O 2) to hydroxyl structures, leading to a reduction in SERS signals. The Au@PNIP-VBA hydrogel has molecular sieve properties and high SERS activity, making it suitable for separation, enrichment, hydrolysis and detection of H 2 O 2 all-in-one. A rapid SERS method was developed for analysis of H 2 O 2 based on the Au@PNIP-VBA hydrogel with the linear range of 8.5 × 10−2-6.8 mg L−1 and the detection limit of 33 μg L−1. The method was successfully applied to the determination of H 2 O 2 residue in fresh milk, pure milk, yogurt and camel milk, with the recoveries were in the range of 82.2%–109.3% and the relative standard deviations were 2.8%–8.3%. This efficient all-in-one strategy has the advantages of simple sample pre-treatment, rapid analysis (30 min) and high sensitivity, making it highly promising for food quality and safety analysis. The core-shell structured Au@PNIP-VBA hydrogel with boronic acid ester group was prepared with high SERS activity and molecular sieves properties, which was used effective separation, enrichment, selective hydrolysis and rapid detection of hydrogen peroxide residue in dairy products all-in-one. [Display omitted] • A novel core-shell structured hydrogel SERS substrate (Au@PNIP-VBA) fabricated. • The hydrogel for separation, enrichment, hydrolysis and detection of H 2 O 2 all-in-one. • A SERS method for analysis of H 2 O 2 by Au@PNIP-VBA hydrogel developed. • The SERS method applied for rapid detection of H 2 O 2 residue in dairy products. [ABSTRACT FROM AUTHOR]

• Phenyl boronic acid was first introduced to NCM/Si-C full cell system. • PBA could be preferentially oxidized and reduced on cathode and anode. • The electrode interfacial property was improved by the robust and B-rich SEI/CEI film. Electrolyte additive engineering is one of the most useful approaches to improve the interfacial property of electrode material in Li-ion batteries (LIBs), which plays a vital role in the mass transport during the electrochemical process. Boron-based electrolyte additives have a good application prospect, however exploring new additives with lower-cost and higher efficiency is still needed. In this study, Phenyl boric acid (PBA), acting as a two-sided interface modifier, is introduced to LiNi 0.5 Co 0.2 Mn 0.3 O 2 /SiC batteries. Through both theoretical calculations and experimental measurements, it is found that PBA could be preferentially reduced on the SiC anode and induce the formation of B-rich solid electrolyte interphase (SEI), which efficiently protects the anode material from the parasitic reaction. Besides, PBA can be sacrificially oxidized before other salt/solvent decomposition, achieving a highly-reversible lithiation/delithiation process at the cathode. Benefiting from these synergistic effects, the target Si-C/Li half-cells achieve a 2.5 % improvement in average coulomb efficiency of 100 cycles, while the capacity retention rate of NCM/Li half-cells and NCM/Si-C full cells increase by 21.4 % and 15.8 %, respectively. This work provides an effective approach to ameliorating the electrode interface chemistry of LIBs employing Si-based anodes Ni-rich cathodes and another analogous battery systems. [ABSTRACT FROM AUTHOR]

[Display omitted] • Fluorescent carbon quantum dots (CQDs) were fabricated via pulsed laser fragmentation in liquid (PLFL). • Further treatment of CQDs through PLFL with an oxidizing agent enhanced their water solubility. • The functionalization of oxidized CQDs with boronic acid (CQDs-APBA) increased their fluorescence emission. • The CQDs-APBA exhibited a fluorescence-off response to glucose at increasing concentrations. Pulsed laser fragmentation in liquid (PLFL) is one of the synthesis routes for producing high-purity carbon quantum dots (CQDs) with less toxic by-products in a straightforward system. The limited literature regarding the application of PLFL-synthesized CQDs motivated this study to exploit them as fluorescent probes in glucose sensing. The design of a fluorescent-based non-enzymatic glucose sensor was achieved by implementing CQDs and 3-aminophenylboronic acid (APBA) as fluorescent probes and glucose receptors, respectively. The CQDs were fabricated by PLFL through an Nd:YAG nanosecond laser, starting from the carbon powder dispersion in ethanol. Then, a laser-assisted post-modification process with oxidizing acid was implemented to produce water-dispersible CQDs for easy functionalization. Functionalizing these CQDs with APBA (CQDs-APBA) resulted in a blue-shifted fluorescence emission with a 35% quantum yield and high photostability. The CQDs-APBA exhibited a turn-off response at increasing glucose concentrations. This method offers good sensitivity with a linear detection range of glucose with a concentration of 0.165 to 8 mM and a detection limit of 165 µM. The applicability of this sensor to real analytes such as saliva obtained a satisfactory result with good reproducibility. This work proves that CQDs synthesized from PLFL can also be an alternative fluorescent probe for fluorescent-based sensing applications. [ABSTRACT FROM AUTHOR]

The design of an intrinsically stabilized ether-free fluoropoly(aryl pyridine) followed by a phosphoric acid pre-swelling strategy provides a high-performance acid-doped membrane for vanadium flow batteries. [Display omitted] • An intrinsically stabilized ether-free fluoropoly(aryl pyridine) was synthesized. • Acid-doped membranes were prepared using a phosphoric acid pre-swelling strategy. • Trade-off between ion conductivity and selectivity was effectively overcome. • Acid-doped membranes exhibit excellent stability and low cost. • Assembled VFB achieves high efficiency and 1500 stable cycles at 120 mA cm−2. Vanadium flow batteries (VFBs) have promising applications for grid-scale energy storage. Unfortunately, the widespread integration of VFBs into large-scale energy storage applications is hindered by the lack of low-cost ion-conducting membranes (ICMs) with high ion selectivity and excellent stability. Herein, we propose an intrinsically stabilized ether-free fluoropoly(aryl pyridine) (PFNP) and provide a high-performance acid-doped membrane via a phosphoric acid pre-swelling strategy for the battery. Effective acid-doping enables the formation of discrete hydrophilic nano-channels within the PFNP matrix, and the extensive internal hydrogen-bonding network synergistically facilitates charge carrier transport. Simultaneously, the close stacking of PFNP chains and the Donnan effect of protonated piperidinium groups within the membrane effectively prevent vanadium ions permeation. Consequently, this innovative strategy allows the acid-doped membrane to achieve nearly perfect ion selectivity (3.64 × 105 S min cm−3), outperforming the Nafion 212 membrane (3.01 × 104 S min cm−3). Moreover, the fluorinated ether-free structure and non-weak-bond pyridine structure impart excellent mechanical and chemical stability to the acid-doped membrane, ensuring long-term membrane operation. As expected, the VFBs assembled with the acid-doped membrane achieve high energy efficiency (83.1 %) at high current density (200 mA cm−2), offer an ultra-low capacity decay rate of 0.08 % per cycle and retain outstanding stability after 1500 cycles at 120 mA cm−2. This study provides a potential material system for commercial VFB membranes by achieving high performance at low cost. [ABSTRACT FROM AUTHOR]

[Display omitted] • As the concentration of the solution increases, boric species undergo polymerization reactions and mutual transformations. • Boric acid molecules coexist with six-membered cyclic triboric acid molecules and cyclic chain tetraboric acid molecules in boric acid solution. • Cyclic tetraboric acid molecules and pentaboric acid molecules are not found in boric acid solution. • Solution pH and cation type have an important influence on the structure of the polymerization products. Boric acid is the common species in borate chemistry and is widely found in nature and Salt Lake brines. It undergoes polymerization reactions to form a variety of borate minerals. However, there are few reports on the polymerization reaction of boric acid molecules in solution to generate polymeric boric acid molecules. In this work infrared spectroscopy technology and quantitative calculation methods were used to study the structure and interconversion of polymeric boric acid molecules in boric acid solution. Research shows that boric acid molecules, six-membered cyclic triboric acid molecules, and ring-chain tetraboric acid molecules exist in boric acid solutions, and as the concentration of the solution increases, these species undergo polymerization reactions and mutual transformations; high degree of polymerization boric species such as bicyclic tetrameric boric acid molecules and pentameric boric acid molecules don't exist in boric acid solutions; Chain dimer boronic acid molecules does not detected in the infrared spectrum. This work provides a new understanding of boron-containing species and their mutual transformation in boric acid solution. [ABSTRACT FROM AUTHOR]

• Binding constant and thermodynamic data of phenylboronic acid derivates and structurally similar linear polyols were investigated through three methods. • The numbers and relative positions of the dihydroxyl group in polyols affected the affinity of the boronic acid group to it. • Intermolecular hydrogen bonding improved the thermodynamic stability of phenylboronate. The separation of 1,2,4-butanetriol (BT) through phenylboronate anion reactive extraction in fermentation broth has been proposed based on the process where the boronic acid groups specifically recognize the cis -dihydroxyl groups. To fully elucidate the affinity process of boronic acid group and polyols, here we have investigated the binding constant (K a) and thermodynamic data of four phenylboronic acid derivates (PBADs) and four structurally similar linear polyols (BT, 1,2-butanediol (1,2-BD), 1,3-butanediol (1,3-BD), and 1,4-butanediol (1,4-BD)) through fluorescence spectroscopy, isothermal titration calorimetry (ITC), and computational chemistry. It can be concluded that the substituent group of boronic acid, the numbers and relative positions of the hydroxyl group in polyols affected the affinity of the boronic acid group to it. The ionized boronic acid group preferentially reacts with the 1,2-dihydroxy group to form a pentacyclic phenylboronate. Compared with other three butanediols, phenylboronic acid (PBA) has a larger binding constant and shows a smaller Gibbs free energy change with BT. [ABSTRACT FROM AUTHOR]

[Display omitted] The immunoproteasome subunit LMP7 (β5i)/LMP2 (β1i) dual blockade has been reported to suppress B cell differentiation and activation, suggesting that the dual inhibition of LMP7/LMP2 is a promising approach for treating autoimmune diseases. In contrast, the inhibition of the constitutive proteasome subunit β5c correlates with cytotoxicity against non-immune cells. Therefore, LMP7/LMP2 dual inhibitors with high selectivity over β5c may be desirable for treating autoimmune diseases. In this study, we present the optimization and discovery of α-amido boronic acids using cryo-electron microscopy (cryo-EM). The exploitation of structural differences between the proteasome subunits led to the identification of a highly selective LMP7/LMP2 dual inhibitor 19. Molecular dynamics simulation based on cryo-EM structures of the proteasome subunits complexed with 19 explained the inhibitory activity profile. In mice immunized with 4-hydroxy-3-nitrophenylacetyl conjugated to ovalbumin, results indicate that 19 is orally bioavailable and shows promise as potential treatment for autoimmune diseases. [ABSTRACT FROM AUTHOR]

Aryl triflate is a type of facile and readily available compounds, which could be used as an alternative substrate for Suzuki cross coupling. In this manuscript, a novel ppm-Pd-Fe/C catalyst has been developed by reduction of palladium acetate and iron trichloride with aqueous hydrazine in situ and supported on activated charcoal. In combined with Sphos, and metal zinc as additives, this catalyst, with 300 ppm palladium loading, could be applied in the Suzuki cross coupling between aryl triflates with aryl neopentylglycolboronates. A highly efficient catalytic aerobic oxidation of alcohols has been developed by using Cu0 nanoparticles in combined with pyridine. With molecular oxygen as terminal oxidant., variety alcohols are oxidized to corresponding ketones or aldehydes under mild conditions. [Display omitted] [ABSTRACT FROM AUTHOR]

• Synthesis of 3,3′-bis(indolyl)methanes from indoles and arylacetic acids in presence of K 2 S 2 O 8 and glucose in water • In-situ oxidation of arylacetic acid to their corresponding carbonyl compounds and coupling with indoles • Metal-free condition with wide substrate scope and good functional group tolerance. An efficient and eco-friendly method has been developed for the preparation of 3,3′-bis(indolyl)methanes from indoles and aryl acetic acid is reported. The in-situ conversion of aryl acetic acid to their corresponding carbonyl compounds, and subsequent condensation with indoles was prompted by K 2 S 2 O 8 in presence of glucose in water. The metal-free reaction with room temperature conditions, and wide substrate scope with good functional group tolerance are the salient features of the developed methodology. [Display omitted] [ABSTRACT FROM AUTHOR]

We prepared the magnetic chitosan carbon quantum dot nanoparticles (Fe 3 O 4 @CQD NPs) via the hydrothermal treatment of chitosan biopolymer and then its magnetization with Fe 3 O 4 nanoparticles. (4-Acetylphenyl)boronic acid compound was utilized for the modification of surface of Fe 3 O 4 @CQD nanoparticles via the covalent imine bond formation between NH 2 groups of chitosan quantum dot with carbonyl functional of acetyl-substituted arylboronic acid. The synthesized Fe 3 O 4 @CQD@AP-B(OH) 2 was characterized by FE-SEM, EDS, XRD, VSM and ICP-OES analysis and its fluorescence property was studied. This magnetic multifunctional nanoplatform sensor has shown high potential sensitivity for Cu2+ ions (in the range of 1.0–30.0 μM with limit of detection 0.3 μM) through interaction of cupric ions with the boronic-acid moiety. [ABSTRACT FROM AUTHOR]

[Display omitted] • NiBr 2 ·DME catalyzed Suzuki-Miyaura coupling reaction of (hetero)aryl halides. • 1,4-bis(2-methylpyridin-4-yl)benzene was used as ligand. • 43 examples of products in 33% to 98% yields. A nickel-catalyzed Suzuki-Miyaura coupling reaction facilitated by pyridine derivative ligands is reported. A wide range of (hetero) aryl halides and (hetero) aryl boronic acids were compatible, which led to the corresponding products in moderate to good yields. A gram-scale reaction proceeded well and afforded an efficient route to prepare methyl [1,1′-biphenyl]-4-carboxylate. [ABSTRACT FROM AUTHOR]

[Display omitted] • NH 2 @Pt with AgVO 3 /PG electrode was fabricated. • 3-Mercaptophenylboronic acid was used as a recognition molecule for glucose. • The reaction mechanism of boronate ester formation and glucose oxidation were scrutinized by DFT calculation. • Insulin-resistant macrophage phenotypes were studied by glucose analysis. • The linkage between insulin resistance and IL-1β release was studied. Glucose is a known energy source that gives insights into cellular metabolism by glycolysis, biosynthesis and glycogen for storage. Investigation of glucose uptake in immune cells requires highly expensive tags and imaging techniques, however, we developed an amine (NH 2) nobbled Platinum (Pt)-Silver vanadate (AgVO 3) sensor platform combined with 3-mercapto phenylboronic acid (MPBA) recognition element for an enzyme-free glucose sensor. Introducing the amine groups into the Pt surface protects the stability of the Pt and extends the longevity of the electrode. A slightly acidic pH-driven boronate ester formation between glucose and boronic acid mechanism and the glucose combined electronic structure of MPBA/NH 2 @Pt/AgVO 3 with its adsorption energy (−2.1 eV) has been scrutinized via density functional theory. Accelerated electron transfer (k s = 4.69 s−1) driven electrochemical oxidation of glucose sensor showed the higher detection limit (LOD) of 61.3 μM and the linear dynamic range from 0.05 to 22.0 mM. Glucose uptake assay of macrophage phenotypes between the healthy and insulin-resistant types provides an in-depth understanding of how glucose impairment affects the macrophage phenotypes. We also observed the M1-type macrophages have uptaken significantly larger amounts of glucose than the others (M0 and M2). [ABSTRACT FROM AUTHOR]

One-step surface functionalization method utilizing boronic acid grafted polyacrylate (PSA-BA). [Display omitted] • Material-independent method for functionalizing biomaterial surfaces. • Coating with boronic acid-grafted polyacrylate offers multifunctionality to surfaces. • Proof-of-concept studies to demonstrate the potential of polyacrylate-based boronic acid functionalization in the field of biomaterials. • Methodology poised to enhance the biomaterial field by offering a superior alternative for surface functionalization. Most biomaterial surfaces are non-functional, which inevitably requires additional functionalization steps. However, these steps are typically material-dependent; only a few limited methods, such as catechol functionalization using polydopamine coating, have been reported as material-independent for surface modification. In this study, we developed a one-step boronic acid surface functionalization method utilizing a polyacrylate that contains both butyl and boronic acid groups. The butyl group exhibits strong interfacial adhesion due to its low glass transition temperature (T g), which maintains its softness and tackiness, thereby facilitating attachment to various substrates. Meanwhile, the boronic acid acts as a functional group for surface modification. Various substrates, including polymers, metals, and ceramics widely used in implants, were successfully coated with the polyacrylate, bestowing boronic acid functionality on the surface. Given that boronic acid is one of the most widely applied functional groups in the biomaterials field, we anticipate that our methodology will be applicable in developing various biomedical applications such as antifouling coatings, biosensors, and bioadhesives. [ABSTRACT FROM AUTHOR]

• DFT calculations show the mechanism details of the palladium-catalyzed defluorinative arylation of gem -difluorocyclopropanes with phenylboronic acids. • The regioselectivity of the reaction originates from electronic effects, while the Z/E -selectivity comes from steric effects. • The chemoselectity is due to the stronger electron-withdrawing capability of the F substituent. DFT calculations were conducted to understand the mechanism details and the Z / E -, regio-, and chemoselectivities observed in the synthesis of 2-fluorinated allylic scaffolds through PdII-catalyzed defluorinative arylation of gem -difluorocyclopropanes with arylboronic acids. The reaction is determined to proceed sequentially through oxidative addition, β -F elimination, two consecutive transmetalations, and reductive elimination. The β -F elimination dictates the Z / E -selectivity, primarily influenced by steric effects, while the reductive elimination step controls the regioselectivities, driven by electronic effects. The second transmetalation process serves as the bottleneck process with a free energy barrier of 26.5 kcal/mol. The chemoselective preference for gem -difluorocyclopropane over its corresponding chloride and its unsubstituted parent is attributed to the stronger electron-withdrawing capability of the F substituent compared to Cl and H, which polarizes the C 1 -C 3 bond more than chlorine or hydrogen, making it easier to break. [Display omitted] [ABSTRACT FROM AUTHOR]

A bioinspired flower-shaped hierarchical teamed boronate affinity (TBA)-imprinted Pt-free micromotor is introduced based on waste rape pollen as a biotemplate with tailor-made specific binding sites. The micromotor exhibits autonomic motor behavior powdered by oxygen bubbles generated by the decomposition of H 2 O 2 for rapid recognition and selective separation of shikimic acid (SA). [Display omitted] • TBA-imprinted Pt-free micromotor is prepared for SA selective adsorption. • The hierarchical flower-shaped structure affords more accessible binding sites. • The micromotor achieves 129.51 mg g−1 with H 2 O 2 under neutral condition. • Self-propelled move behavior and TBA imprinted strategy facilitate the separation. Self-propelled nano/micromotors are the frontier of separation materials because they are capable of converting external energy in the surrounding environment into kinetic energy for their autonomous movement. In this work, a bioinspired flower-shaped hierarchical teamed boronate affinity (TBA)-imprinted Pt-free micromotor is introduced based on waste rape pollen as a biotemplate due to its merits of sustainability, rich source and low price. Such micromotor, composed of MnO 2 nanosheets as a catalytic medium, Mg-Al layered double hydroxides (LDH) nanosheets as large surface area substrate and functional surface imprinted polymers as identification subject, exhibits autonomic motor behavior powdered by oxygen bubbles generated by the decomposition of H 2 O 2 and selective recognition and separation for shikimic acid (SA). The hierarchical flower-shaped structure affords more accessible recognition sites for SA, which thus facilitates the separation process. Moreover, in combination with the TBA strategy, the micromotor can precisely recognize the target via boronate affinity between boronic acids and cis -diols from SA while showing strong binding capacity under neutral conditions. Benefiting from the pluripotent role of pioneering boronate affinity-covalent imprinted technique, hydrogen bonds interaction and nanoconfinement effect, the resulting affinity towards SA is evidently enhanced with maximal adsorption capacity of 129.51 mg g−1 at neutral pH in the presence of H 2 O 2. After five capture/release cycles, the adsorption capacity remains above 85 %. This proposed flower-shaped hierarchical micromotor expands the scope of potential materials for the adsorption of SA and provides a new and promising direction for fabricating adsorbents applied in the separation and purification of natural products. [ABSTRACT FROM AUTHOR]

The utilization of environmentally friendly biopolymer poly(3-hydroxybutyrate) (PHB) as a support for metal catalysts is an unexplored area. Herein, exploring a stable and recyclable heterogeneous palladium (Pd) catalyst based on PHB for the Suzuki cross-coupling reaction is an attractive topic. In this study, a novel hydrophobic and hydrophilic core-shell bead supported Pd catalyst was developed by using PHB particle as a hydrophobic rigid framework and hydrophilic chitosan (CS) as the surface of the supporting matrix to fix Pd nanoparticles (NPs). The results of structural characterization showed that the Pd NPs with a small average size of 2.3±0.82 nm were uniformly distributed on the surface of the PHB-CS bead with a Pd content of 0.12 wt%. This demonstrated that the PHB-CS bead created a stable coordinated environment for Pd fixation, preventing Pd leaching. Furthermore, the Pd@PHB-CS catalyst exhibited high catalytic efficiency with 99% conversion in the Suzuki reaction under mild conditions, using a solvent mixture of EtOH and H 2 O (1:1) for 0.5 h. It is worth noting that the catalyst exhibits excellent stability, and reusability. It can be reused five times without a significant reduction in activity while maintaining its structural integrity. The successful construction of Pd@PHB-CS will further expand the application of PHB in the field of metal catalysts and may help establish a green and environmentally friendly multifunctional platform for the development of highly recyclable heterogeneous catalysts containing metal nanoparticles. [Display omitted] • A novel hydrophobic/hydrophilic core-shell bead supported-Pd catalyst was easily fabricated. • PHB serves as a hydrophobic core to stabilize the catalyst and prevent water from damaging its structure. • Chitosan serves as a hydrophilic surface to immobilize Pd, making the catalyst highly dispersed. • The catalyst exhibits excellent stability, catalytic efficiency, and reusability. [ABSTRACT FROM AUTHOR]

Human carbonic anhydrases (hCAs) play a central role in various physiological processes in the human body. HCAs catalyze the reversible hydration of CO 2 into HCO 3 −, and hence maintains the fluid and pH balance. Overexpression of CA II is associated with diseases, such as glaucoma, and epilepsy. Therefore, CAs are important clinical targets and inhibition of different isoforms, especially hCA II is used in treatment of glaucoma, altitude sickness, and epilepsy. Therapeutically used CA inhibitors (CAI) are sulfonamide-based, such as acetazolamide, dichlorphenamide, methazolamide, ethoxzolamide, etc. However, they exhibit several undesirable effects such as numbness, tingling of extremities, malaise, metallic taste, fatigue, renal calculi, and metabolic acidosis. Therefore, there is an urgent need to identify safe and effective inhibitors of the hCAs. In this study, different phenyl boronic acids 1 – 5 were evaluated against bovine (bCA II) and hCA II. Among all, compound 1 (4-acetylphenyl boronic acid) was found to be active against bCAII and hCA II with IC 50 values of 246 ± 0.48 and 281.40 ± 2.8 μ M, respectively, while the remaining compounds were found in-active. Compound 1 was identified as competitive inhibitor of hCA II enzyme (K i = 283.7 ± 0.002 μM). Additionally, compound 1 was found to be non-toxic against BJ Human fibroblast cell line. The X-ray crystal structure for hCA II in-complex with compound 1 was evaluated to a resolution of 2.6 Å. In fact, this the first structural analysis of a phenyl boron-based inhibitor bound to hCA II, allowing an additional structure-activity analysis of the compounds. Compound 1 was found to be directly bound in the active site of hCA II by interacting with His94, His119, and Thr199 residues. In addition, a bond of 3.11 Å between the zinc ion and coordinated boron atom of the boronic acid moiety of compound 1 was also observed, contributing to binding affinity of compound 1 for hCA II. 8IGF. [ABSTRACT FROM AUTHOR]

Effect-directed analysis (EDA) has been increasingly used for screening toxic contaminants in the environment, but conventional EDA procedures are often time-consuming and labor-extensive. This challenges the use of EDA for toxicant identification in the scenarios when quick answers are demanded. Herein, a solid phase microextraction ligand fishing (SPME-LF) strategy has been proposed as a rapid EDA approach for identifying acetylcholinesterase (AChE) active compounds in water. The feasibility of ligand fishing techniques for screening AChE active chemicals from environmental mixtures was first verified by a membrane separation method. Then, SPME fibers were prepared through self-assembly of boronic acid groups with AChE via co-bonding and applied for SPME-LF. As AChE coated SPME fibers selectively enriched AChE-active compounds from water, comparing sorbing compounds by the SPME fibers with and without AChE coating can quickly distinguish AChE toxicants in mixtures. Compared with conventional EDA, SPME-LF does not require repeating sample separations and bioassays, endowing SPME-LF with the merits of low-cost, labor-saving, and user-friendly. It is believed that cost-efficient and easy-to-use SPME-LF strategy can potentially be a rapid EDA method for screening receptor-specific toxicants in aquatic environment, especially applicable in time-sensitive screening. [Display omitted] • Ligand fishing is potentially used as a rapid effect-based analytical method. • AChE was immobilized on SPME fibers by self-assembly of boronic acid groups. • SPME-based ligand fishing method selectively adsorbed AChE active contaminants in water. [ABSTRACT FROM AUTHOR]

• Non-symmetric Pd(II) NNS and NNSe pincers are highly robust in DMF/water mixtures at 110 °C. • The catalytic activity of Pd(II) NNS pincers in S M coupling is boosted by Micro-wave activation. • NNS Pd(II) pincer including a SMe pending ligand selectively catalyzed nitrile hydration of 4-bromonitrobenzene in mild conditions. • NNSe pincers decompose under microwave irradiation in S M catalytic conditions. • Iminophosphorane based Pd(II) NNS pincers are more robust and efficient catalysts than related orthometalated CNS pincers. In this work, four non-symmetric NNS and NNSe Pd(II) pincer complexes (1 – 4) supported by tridentate iminophosphorane ligands bearing both the indole heterocycle and a varying chalcogenoether pending arm [XR = SPh (1), SePh (2), SMe (3), SeMe (4)], were tested in the Suzuki–Miyaura cross coupling reaction between phenyl boronic acid and aryl bromides. The modest catalytic activity of complex 3 under conventional heating (110 °C), was boosted under microwave irradiation, leading to excellent yields of coupling under remarkably mild conditions: 5 min of reaction time at 70 °C under aerobic conditions. The complexes supported by NNS ligands proved to be more robust and superior catalysts than those supported by NNSe ligands, which tended to decompose under catalytic conditions. Among the four complexes, pincer 3 also demonstrated the best catalytic performance and was tested in the coupling of a variety of p -substituted bromobenzenes. Notably, the catalytic performance of complex 3 was unaffected by the mercury drop test, and with most substrates in the series, the percentage of conversion reflected the aryl bromides' Hammett parameter. However, little to no coupling occurred with p -bromobenzonitrile and the reaction with p -nitrobenzene resulted in only limited conversion yields. In fact, when activated using microwave irradiation, complex 3 unexpectedly catalyzed the nitrile hydration of p -bromobenzonitrile, yielding p -bromobenzamide under remarkably mild Suzuki–Miyaura coupling conditions. Non-symmetric Pd(II) NNS pincer supported by a tridentate iminophosphorane ligand, bearing the indole and a SeMe pending arm, is both an excellent catalyst in S M cross-coupling and selective hydration of 4-bromonitrobenzene in mild conditions under MW irradiation. [Display omitted] [ABSTRACT FROM AUTHOR]

• Nickel catalyst outperforms palladium in terms of the activation barrier for both oxidative addition and transmetalation steps. • The rate-determining step of the reaction is the transmetalation step, with an activation energy of 65.22 kJ/mol for nickel catalysts and 74.17 kJ/mol for palladium catalysts. • Findings suggest that single-atom nickel can effectively replace palladium as a catalyst for Suzuki-Miyaura cross-coupling reaction in biphenyl synthesis. [Display omitted] The present study employed density-functional theory (DFT) calculations to investigate the Suzuki-Miyaura Cross-Coupling reaction using single atom catalysis (SAC) via palladium or nickel. The reaction barriers for chloride bond cleavage in phenyl chloride were found to be 34.68 kJ/mol and 24.24 kJ/mol for palladium and nickel catalysts, respectively. During the transmetalation process, the energy barrier for boronic acid release from phenyl trihydroxyboronate using SAC via palladium and SAC via nickel was calculated to be 74.17 kJ/mol and 65.22 kJ/mol, respectively. The results indicated that in the oxidative addition and transmetalation reactions, the SAC via nickel outperforms the SAC via palladium, while the SAC via palladium excels in the reductive elimination reaction. These findings suggest the effectiveness of a SAC via nickel as a substitute for SAC via palladium in the synthesis of biphenyls in the Suzuki-Miyaura cross-coupling reaction. [ABSTRACT FROM AUTHOR]

[Display omitted] • First report on synthesis of stereoenriched bis glyco-cyclohexylamines via dual Petasis Reaction. • Plausible mechanism; OH directed anti -diastereoselection; N,N'-chelate organoboron complex assisted syn -diastereoselection. • Structures elucidated using HMBC, HSQC NMR experiments and X-ray single crystallographic analysis. • An efficient and simple catalyst free one pot synthesis using readily available inexpensive compounds was done. • All new compounds have been documented with good yields along with high de/ee. A one pot dual Petasis reaction of sugars, styrene boronic acid and cis / trans 1,2-cyclohexyldiamines was done for the diastereoselective synthesis of bis glyco-cyclohexylamines, which have multiple stereogenic centres and coordinating heteroatoms. The products were isolated and characterized by HMBC, HSQC, 1H, 13C NMR experiments, and X-ray single crystallographic analysis. A plausible mechanism for the dual Petasis reaction was proposed that involves hydroxyl directed borylation for the anti- diastereoselectivity along with N,N'-chelate tetracoordinated organoboron complex assisted syn- diastereoselectivity based on the experimental findings. [ABSTRACT FROM AUTHOR]

A Cu/hydrazone catalysis has been applied in the oxidative coupling of arylboronic acids and aromatic thiols for the synthesis of asymmetric diaryl sulfides. The reaction features mild conditions and readily available hydrazone ligand. It works for a wide range of arylboronic acids and thiols with good to excellent yields, exhibiting great potential of such hydrazone ligands in organic synthesis. [Display omitted] [ABSTRACT FROM AUTHOR]

Highly toxic alpha emitting radionuclide, astatine-211 (211At) labelled compounds have great potential as therapeutic radiopharmaceuticals in the targeted alpha therapy (TAT). Previously we have reported the usefulness of copper (Cu)-mediated radiobromination and radioiodination via boronic acid precursors for the synthesis of 77Br/125I-labelled prostate-specific membrane antigen (PSMA) inhibitors which are applicable as imaging probes. This background led us to investigate the Cu-mediated astatination of PSMA inhibitors as a therapeutic agent for TAT. 211At-labelled PSMA inhibitors [211At] m AtB-PS were synthesised from a boronic acid precursor with tert -butoxycarbonyl groups by Cu-mediated astatination in the presence or absence of NaOH and K 2 CO 3 in order to stabilize the chemical form of At followed by deprotection of the tert -butoxycarbonyl group under acidic condition. We confirmed that [211At] m AtB-PS 1a was synthesised in 64.4–87.3% of overall radiochemical yields (RCYs). These yields are considered acceptable regardless of basic salts existed. However, RCYs of the astatination step differed depending on the base (NaOH: 22.0–31.9%; K 2 CO 3 ; 69.0–82.3%; without base: 60.1%). We speculated that astatination also occurred during the deprotection of tert -butoxycarbonyl groups. This could be attributed to an electrophilic deboronation reaction involving electrophilic astatine species, resulting in compensation for the overall RCYs. Cu-mediated astatination via boronic precursors was found to be very effective for the synthesis of 211At-labelled PSMA inhibitors for the targeted alpha therapy. 211At-labelled PSMA inhibitor [211At] m AtB-PS was synthesised via the copper-mediated astatination followed by deprotection of tert -butoxycarbonyl groups with acid in good to excellent radiochemical yields (RCYs). [Display omitted] [ABSTRACT FROM AUTHOR]

Boronic ester is a kind of well-known and active dynamic covalent bond. Yet preparing an even boronic ester crosslinked network from boronic acid containing polymers and diol containing polymers remains challenging because the reaction between boronic acid and diol is so fast that by now blocking boronic acid/diol (in the form of boronic ester) or special mixing procedures are required. In this work, a series of storage-stable and self-crosslinkable combinations of boronic acid (2%, 4% and 8%, in mol%) containing polymer latices and diol (2%, 4% and 8%, in mol%) containing polymer latices were achieved by simply mixing the two types of latex dispersions, without using any blocking agents or special mixing procedure. In the combined dispersions, water as the dispersion medium isolates boronic acids and diol groups, while the combined latices can undergo self-crosslinking via the encounters of boronic acid and diols during the latex film formation. Target synthetic products were confirmed by NMR, UV–vis absorption, FT-IR and Raman spectra. The combined latices have simultaneously ensured the even mixing, storage stability (60 °C for 1 month or room temperature for 6 months) and self-crosslinking (examined by DMA, Soxhlet extractions, up to 97% gel content). The method of respectively preparing and then combining the two types of latices is better in polymerization stability, toughness, and wettability, compared to the method of preparing crosslinked polymer with the comparable divinyl boronic ester crosslinker (1%, 2% and 4%, in mol%). With these advantages, the method of combining such highly reactive polymers by utilizing the features of latex systems is inspirational in the fields of colloid science and reactive polymers. [Display omitted] • Direct mixing of boronic acid containing polymers and diol containing polymers. • The VBA/GMMA latex combinations possess excellent storage stability. • The VBA/GMMA latex combinations establish self-crosslinking while being filmed. [ABSTRACT FROM AUTHOR]

• One-pot hydrothermal strategy was used to prepared magnetic adsorbent. • The MNC contained abundant functional groups and presented good magnetic properties. • Multiple forces co-contributed to the capture of MNC/MSPE towards Se(IV)/OPA complex. • Sensitive and reliable method for the speciation of Se(IV) and Se(VI) was developed. Speciation of Se(IV) and Se(VI) is essential due to their significant differences in reactivity, toxicity and bioavailability. Efficient extraction is the pivotal step in the quantification of inorganic selenium species. In this work, a new magnetic nano-composite (MNC) containing boronic acid group and aminated multwalled carbon nanotubes was facilely fabricated by means of one-pot hydrothermal strategy. The prepared MNC contained abundant functional groups and satisfactory magnetic saturation value. Combining with magnetic solid phase extraction (MSPE) format, the MNC displayed satisfactory capture performance towards the complex formed by the coordination of Se(IV) and o -phenylenediamine (OPA). Adsorption isotherm and adsorption kinetics were studied in detail to investigate the adsorption procedure of Se(IV)/OPA complex on MNC. Under the optimal preparation conditions of MNC and extraction parameters, the MNC/MSPE was connected with HPLC equipped with a diode array detector (DAD) to quantify trace Se(IV) and Se(VI) species in water and milk samples. Se(VI) was reduced to Se(IV) and then the total inorganic Se was quantified by the developed method. Subtraction method was used to measure the concentration of Se(VI). The achieved limits of detection were in the ranges of 0.0082–0.013 μg/L and 0.041–0.13 μg/kg for water and milk samples, respectively. Recoveries in actual samples spiked with different amounts of analytes varied from 81.0 % and 117 %, and the RSDs for repeatability varied from 1.0 % to 10 %. In comparison with existing studies based on MSPE, the established method presents some merits such as greenness in the preparation of magnetic adsorbent, rapid extraction procedure, low cost and satisfactory sensitivity in the speciation of inorganic Se species. [ABSTRACT FROM AUTHOR]

Dynamic fluctuation of circulating tumor cells (CTCs) can serve as an indicator of tumor progression. However, the sensitive isolation of CTCs remains extremely challenging due to their rarity and heterogeneity. Against this dilemma, dendritic boronic acid-modified magnetic nanoparticles (MNPs) were prepared in this study, and polyethyleneimine (PEI) was utilized as a scaffold to significantly increase the number of boronic acid moieties. Then the novel developed material was applied to monitor the number of CTCs in mice with metastatic breast cancer to evaluate the therapeutic effects of matrine (Mat), doxorubicin (Dox), and Mat in combination with Dox. Compared to the low binding capacity of a single boronic acid ligand, dendritic boronic acid shows enhanced sensitivity in binding to sialic acid (SA), which is overexpressed in CTCs. The results showed that the capture efficiency of this modified material could achieve 94.7% and successfully captured CTCs in blood samples from mice with metastatic breast cancer. The CTC counts were consistent with the results of the pathologic examination, demonstrating the reliability and utility of the method. The dendritic boronic acid nanomaterials prepared in this study showed high specificity, sensitivity, and accuracy for cancer cell capture. The approach is expected to provide new insights into cancer diagnosis, personalized therapy, and optimization of treatment regimens. [Display omitted] • A simple CTC isolation material with high capture efficiency, specificity, sensitivity, and accuracy was developed. • Multilocus-boronic acid functionalized MNPs substantially enhance the capture efficiency of CTCs. • The CTC count is consistent with the results of pathological examination and is an accurate and practical method. • The CTCs count can assess the efficacy of drugs and provide new insights into their clinical application. [ABSTRACT FROM AUTHOR]