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Acrylic coatings were prepared by UV-induced crosslinking of two different diacrylates monomers having the same molecular weight but containing either a polyethyleneoxide chain or an alkylic chain. The fouling behavior of the UV-cured coatings was assessed by a simple set-up, monitoring the growth of Rhodotorula mucilaginosa and the adhesion of the film formed by the pigmented yeast. The effect of the monomers, the photoinitiator and the polymers on the microorganism growth was studied. The monomers partially inhibited the yeast growth, the coatings showed fouling release activity: yeast could grow on both coatings but with different morphology, they detached easily by washing depending on the type of coating. By examining the physico-chemical and mechanical properties of the polymers, the fouling release behavior was found mostly dependent on the surface tension and the water uptake of the coatings. [ABSTRACT FROM AUTHOR]

• Fusion of Pc4CL1 and VvSTS facilitated the accumulation of resveratrol. • Redirecting metabolic flux of the precursor increased resveratrol production. • Multi-copy integration of key genes and knockout of DGA1 facilitated the process. • Y. lipolytica performed best for resveratrol production in the yeast morphology. • The highest titer for microbially produced resveratrol (22.5 g/L) reported to date. Resveratrol is a polyphenol with numerous applications in food, pharma, and cosmetics. Lack of precursors and low titer are the main problems hindering industrial scale resveratrol production. Based on previous prescreening, expressing the combination of FjTAL , Pc4CL1 and VvSTS achieved the best resveratrol titer. This was further improved to 235.1 mg/L through engineering the shikimic acid pathway, applying a modular enzyme assembly of Pc4CL1 and VvSTS , enhancing p -coumaric acid supply and diverting glycolytic flux toward erythrose-4-phosphate. The titer was increased to 819.1 mg/L following two rounds of multicopy integration of resveratrol biosynthesis and malonyl-CoA supply, respectively. The titer reached 22.5 g/L with a yield on glucose of 65.5 mg/g using an optimum fed-batch strategy in a 5 L bioreactor with morphology control. This research is the highest report on the de novo production of resveratrol in Yarrowia lipolytica and the findings lay a solid foundation for other producing polyphenols. [ABSTRACT FROM AUTHOR]