1. A single dual-emissive nanofluorophore test paper for highly sensitive colorimetry-based quantification of blood glucose
- Author
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Yujie Zhou, Bianhua Liu, Cui Liu, Ming-Yong Han, Liying Zhang, Shuhu Du, Ruilong Zhang, Zhongping Zhang, and Xiaoyan Huang
- Subjects
Blood Glucose ,Paper ,Brightness ,Silicon dioxide ,Biomedical Engineering ,Biophysics ,Analytical chemistry ,Biosensing Techniques ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,chemistry.chemical_compound ,Electron transfer ,Limit of Detection ,Quantum Dots ,Cadmium Compounds ,Electrochemistry ,Humans ,Colorimetry ,Fluorescent Dyes ,Detection limit ,Chemistry ,General Medicine ,Silicon Dioxide ,021001 nanoscience & nanotechnology ,Fluorescence ,0104 chemical sciences ,Spectrometry, Fluorescence ,Covalent bond ,Quantum dot ,Tellurium ,0210 nano-technology ,Biotechnology - Abstract
Fluorescent test papers are promising for the wide applications in the assays of diagnosis, environments and foods, but unlike classical dye-absorption-based pH test paper, they are usually limited in the qualitative yes/no type of detection by fluorescent brightness, and the colorimetry-based quantification remains a challenging task. Here, we report a single dual-emissive nanofluorophore probe to achieve the consecutive color variations from blue to red for the quantification of blood glucose on its as-prepared test papers. Red quantum dots were embedded into silica nanoparticles as a stable internal standard emission, and blue carbon dots (CDs) were further covalently linked onto the surface of silica, in which the ratiometric fluorescence intensity of blue to red is controlled at 5:1. While the oxidation of glucose induced the formation of Fe(3+) ions, the blue emission of CDs was thus quenched by the electron transfer from CDs to Fe(3+), displaying a serial of consecutive color variations from blue to red with the dosage of glucose. The high-quality test papers printed by the probe ink exhibited a dosage-sensitive allochromatic capability with the clear differentiations of ~5, 7, 9, 11mM glucose in human serum (normal: 3-8mM). The blood glucose determined by the test paper was almost in accordance with that measured by a standard glucometer. The method reported here opens a window to the wide applications of fluorescent test paper in biological assays.
- Published
- 2016
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