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We have developed a method, based on micellar liquid chromatography, to determine albendazole and ivermectin in dairy products and biological waste from bovine, as well as edible tissues from poultry. Anthelmitics were resolved in less than 10 min using a C18 column and a mobile phase of 0.15 mol/L sodium dodecyl sulfate – 6% 1-pentanol at buffered at pH 7 with a 0.01 M phosphate salt, running under isocratic mode at 1 mL/min. Detection was by absorbance at 292 nm. Method was successfully validated following official validation guidelines, in terms of: selectivity, sensitivity, calibration range (0.0125−0.5 mg/kg to 25−50 mg/kg), linearity (r2 > 9990), trueness (86.3–105.6%), precision (

A Micellar Liquid Chromatographic method is described to determine Rifampicin and Rifabutin in plasma from Tuberculosis patients. Samples were diluted in mobile phase and then directly injected, avoiding long and tedious extraction steps. The analytes were resolved from the matrix without interferences from endogenous compounds using a mobile phase of sodium dodecyl sulfate 0.15 mol L-1–6%(v/v) 1-pentanol and phosphate buffer at pH 3, running at 1 mL min−1 through a C18 column at 25 °C. Detection was carried out by UV absorbance at 270 nm. Under these conditions, the final chromatographic analysis time was 22 min. The analytical methodology was validated following the FDA 2018 Bioanalytical Method Validation Guidance for Industry. The response of the drugs in plasma was linear in the 0.05–5 μg/mL range, with r 2 > 0.9993. Trueness and precision were

The performance of a commercially available electrochemical detector cell has been evaluated for use in capillary electrophoresis (CE). It was shown that with proper installation of the capillary, the zone broadening caused by the cell is limited by the depletion of the analytes on the electrode surface. Despite the relatively large geometric volume of the cell, its contribution to zone widths was therefore only in the order of 1–2 s. When coupled to a CE set-up, plate numbers up to 100 000 could be obtained. With a 1-mm diameter glassy carbon electrode the detector noise was 3–5 pA, depending on the applied separation voltage. With this noise level detection limits of 10−8 mol l−1 could be obtained. The cell has been used for the determination of catecholamines, chlorophenols and o-phthalaldehyde derivatives of amino acids.