1. Synthesis and characterisation of new fluorescent Na+ and K+ indicators
- Author
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Georges J. Hoornaert, Ashty Saleh, Els Cielen, Noël Boens, Abdellah Tahri, and Katja Ver Heyen
- Subjects
Fluorophore ,Aqueous solution ,Organic Chemistry ,Inorganic chemistry ,Ether ,Pyrazoline ,Biochemistry ,Fluorescence ,Dissociation constant ,chemistry.chemical_compound ,chemistry ,Drug Discovery ,Qualitative inorganic analysis ,Carboxylate - Abstract
The synthesis of new fluorescent indicators for Na+, Benz-15-5 or Diaz-15-5, and for K+, Benz-18-6 or Diaz-18-6, respectively, is reported. They consist of a benzocrown or a diazacrown ether linked to a pyrazoline fluorophore substituted with carboxylate groups to ensure water solubility. They were obtained via reaction of an α,β-unsaturated ketone with an aryl hydrazine. The fluorescence intensity of Benz-15-5 is nearly insensitive to Na+. Benz-18-6 displays a four-fold selectivity for K+ over Na+, with a dissociation constant of 3.6 mM for the K+: indicator complex. Diaz-15-5 and Diaz-18-6 have only a weak affinity for monovalent cations, so that they only can be used for the determination of high Na+ and K+ concentrations.
- Published
- 1999
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