1. Differential effects of high and low strength magnetic fields on mouse embryonic development and vasculogenesis of embryonic stem cells
- Author
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Nabil K. El-Fiky, Ibrahim K. El-Shourbagy, Andreas Finkensieper, Mohamed M. Bekhite, Maria Wartenberg, Heinrich Sauer, Khaled M. Omar, and Fouad A. Abou-Zaid
- Subjects
Vascular Endothelial Growth Factor A ,0301 basic medicine ,Embryonic Development ,Neovascularization, Physiologic ,macromolecular substances ,Embryoid body ,Biology ,Eye ,Weight Gain ,Toxicology ,Crown-Rump Length ,Cell Line ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,Vasculogenesis ,Pregnancy ,medicine ,Animals ,skin and connective tissue diseases ,Lung ,Embryoid Bodies ,Mice, Inbred BALB C ,Fetus ,Embryogenesis ,Mouse Embryonic Stem Cells ,Embryo, Mammalian ,Embryonic stem cell ,Cell biology ,Vascular endothelial growth factor ,Vascular endothelial growth factor A ,Magnetic Fields ,030104 developmental biology ,medicine.anatomical_structure ,Liver ,chemistry ,embryonic structures ,Immunology ,Embryo Loss ,Female ,Reactive Oxygen Species ,Blood vessel - Abstract
Man-made magnetic fields (MFs) may exert adverse effects on mammalian embryonic development. Herein, we analysed the effect of 10mT 50Hz sinusoidal (AC) or static (DC) MFs versus 1mT MFs on embryonic development of mice. Exposure for 20days during gestation to 10mT MFs increased resorptions and dead fetuses, decreased crown-rump length and fresh weight, reduced blood vessel differentiation and caused histological changes, accompanied with diminished vascular endothelial growth factor (VEGF) protein expression in several organs. In embryonic stem (ES) cell-derived embryoid bodies exposure towards 10mT MFs increased reactive oxygen species (ROS), decreased vascular marker as well as VEGF expression and enhanced apoptosis. In conclusion, our combined data from in vivo and in vitro experiments identified VEGF as an important mediator during embryonic development that can be influenced by high strength MFs, which in consequence leads to severe abnormalities in fetus organs and blood vessel formation.
- Published
- 2016
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