1. Germline variants in DNA repair genes are associated with young-onset head and neck cancer
- Author
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Luiz Paulo Kowalski, Luisa Matos do Canto, Clovis Antonio Lopes Pinto, Mads M. Aagaard, Priscila M Miranda, Silvia Regina Rogatto, Sarah Santiloni Cury, Fabio Albuquerque Marchi, Annabeth Høgh Petersen, Thiago Celestino Chulam, University of Southern Denmark, Universidade Estadual Paulista (UNESP), CIPE – A.C.Camargo Cancer Center, and A.C.Camargo Cancer Center
- Subjects
Adult ,Cancer Research ,DNA Copy Number Variations ,DNA Repair ,Somatic cell ,Biology ,Germline ,Cancer predisposition ,Oral cavity carcinomas ,stomatognathic system ,FANCG ,CDKN2A ,medicine ,Genetic predisposition ,Humans ,Genetic Predisposition to Disease ,Copy-number variation ,neoplasms ,Germ-Line Mutation ,Exome sequencing ,Squamous Cell Carcinoma of Head and Neck ,Head and neck cancer ,Oropharynx carcinomas ,Middle Aged ,medicine.disease ,Early-onset cancer ,stomatognathic diseases ,Germ Cells ,Risk factors ,Oncology ,Head and Neck Neoplasms ,Whole-exome sequencing ,Cancer research ,Oral Surgery - Abstract
Made available in DSpace on 2022-04-28T19:45:14Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-11-01 The genetic predisposition to head and neck carcinomas (HNSCC) and how the known risk factors (papillomavirus infection, alcohol, and tobacco consumption) contribute to the early-onset disease are barely explored. Although HNSCC at early onset is rare, its frequency is increasing in recent years. Germline and somatic variants were assessed to build a comprehensive genetic influence pattern in HNSCC predisposition and patient outcome. Whole-exome sequencing was performed in 45 oral and oropharynx carcinomas paired with normal samples of young adults (≤49 years). We found FANCG, CDKN2A, and TPP germline variants previously associated with HNSCC risk. At least one germline variant in DNA repair pathway genes was detected in 67% of cases. Germline and somatic variants (including copy number variations) in FAT1 gene were identified in 9 patients (20%) and 12 tumors (30%), respectively. Somatic variants were found in HNSCC associated genes, such as TP53, CDKN2A, and PIK3CA. To date, 55 of 521 cases from the large cohort of TCGA presented < 49 years old. A comparison between the somatic alterations of TCGA-HNSCC at early onset and our dataset revealed strong similarities. Protein-protein interaction analysis between somatic and germline altered genes revealed a central role of TP53. Altogether, germline alterations in DNA repair genes potentially contribute to an increased risk of developing HNSCC at early-onset, while FAT1 could impact the prognosis. Department of Clinical Genetics University Hospital of Southern Denmark Vejle Institute of Regional Health Research University of Southern Denmark Department of Structural and Functional Biology São Paulo State University (UNESP) International Research Center CIPE – A.C.Camargo Cancer Center Department of Head and Neck Surgery and Otorhinolaryngology A.C.Camargo Cancer Center Department of Pathology A.C.Camargo Cancer Center Department of Structural and Functional Biology São Paulo State University (UNESP)
- Published
- 2021
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