1. A polyphenol-enriched fraction of Cyclopia intermedia decreases lipid content in 3T3-L1 adipocytes and reduces body weight gain of obese db/db mice
- Author
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Elizabeth Joubert, Johan Louw, Christiaan J. Malherbe, Barbara Huisamen, Babalwa Jack, Kwazi B. Gabuza, Alexandra E. Schulze, Christo J. F. Muller, Carmen Pheiffer, and Sithandiwe E. Mazibuko-Mbeje
- Subjects
0301 basic medicine ,Aqueous solution ,biology ,Chemistry ,food and beverages ,Fraction (chemistry) ,3T3-L1 ,Plant Science ,Cyclopia ,medicine.disease ,biology.organism_classification ,03 medical and health sciences ,030104 developmental biology ,Nutraceutical ,Biochemistry ,Polyphenol ,medicine ,Viability assay ,Food science ,Cyclopia intermedia - Abstract
Extracts of Cyclopia species, indigenous South African fynbos plants used for the production of honeybush tea, have potential as anti-obesity nutraceutical ingredients. Previously, we demonstrated that aqueous extracts of C. maculata and C. subternata exhibited anti-obesity effects in 3T3-L1 adipocytes. In this study, we further explored these anti-obesity effects of C. maculata and C. subternata as well as C. intermedia for the first time. Extracts were prepared using a 40% methanol–water mixture (40% MeOH) in order to enhance the polyphenolic content of the extracts. Moreover, these extracts were separated into aqueous and organic fractions using liquid–liquid partitioning with n-butanol and water to further enrich the polyphenol content of the organic fractions. Extracts of all three Cyclopia species decreased the lipid content in 3T3-L1 adipocytes, although differences in bioactivity of their aqueous and organic fractions were observed. The organic fraction of C. intermedia was further investigated. This fraction dose-dependently decreased the lipid content in 3T3-L1 adipocytes without affecting cell viability, while increasing mRNA expression of HSL (1.57-fold, P
- Published
- 2017
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