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23 results on '"Ballerini, Laura"'

7. Exploiting natural polysaccharides to enhance in vitro bio-constructs of primary neurons and progenitor cells

Author

Medelin, Manuela, primary, Porrelli, Davide, additional, Aurand, Emily Rose, additional, Scaini, Denis, additional, Travan, Andrea, additional, Borgogna, Massimiliano Antonio, additional, Cok, Michela, additional, Donati, Ivan, additional, Marsich, Eleonora, additional, Scopa, Chiara, additional, Scardigli, Raffaella, additional, Paoletti, Sergio, additional, and Ballerini, Laura, additional

Published
2018
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13. TEMPERATURE-RESPONSIVE MATERIALS MAY ACT AS EXTRACELLULAR MATRIX AND CELL VEHICLE FOR CARDIAC TISSUE ENGINEERING

Author

Castellanos, Brisa Pena, primary, Park, Daewon, additional, Long, Carlin S., additional, Martinelli, Valentina, additional, Bosi, Susanna, additional, Ballerini, Laura, additional, Prato, Maurizio, additional, Sucharov, Carmen, additional, Jeong, Mark, additional, Taylor, Matthew R.G., additional, Shandas, Robin, additional, and Mestroni, Luisa, additional

Published
2015
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14. Injectable Reverse Thermal Gel Biopolymers may Act as an Extracellular Matrix and Cell Vehicle for Cardiac Tissue Engineering

Author

Pena Castellanos, Brisa M., primary, Park, Daewon, additional, Long, Carlin S.S., additional, Martinelli, Valentina, additional, Bosi, Susanna, additional, Ballerini, Laura, additional, Prato, Maurizio, additional, Sucharov, Carmen, additional, Jeong, Mark, additional, Taylor, Matthew R.G., additional, Shandas, Robin, additional, and Mestroni, Luisa, additional

Published
2015
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16. CARBON NANOTUBES INSTRUCT PHYSIOLOGICAL GROWTH AND FUNCTIONALLY MATURE SYNCYTIA: NON-GENETIC ENGINEERING OF CARDIAC MYOCYTES

Author

Mestroni, Luisa, primary, Martinelli, Valentina, additional, Cellot, Giada, additional, Toma, Francesca M., additional, Long, Carlin, additional, Caldwell, John, additional, Zentilin, Lorena, additional, Giacca, Mauro, additional, Turco, Antonio, additional, Prato, Maurizio, additional, and Ballerini, Laura, additional

Published
2013
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21. Carbon based substrates for interfacing neurons: Comparing pristine with functionalized carbon nanotubes effects on cultured neuronal networks

Author

Maurizio Prato, Laura Ballerini, Cristina Cantarutti, Marko Mihajlovic, Alessandra Fabbro, Susanna Bosi, Bosi, Susanna, Fabbro, Alessandra, Cantarutti, Cristina, Mihajlovic, Marko, Ballerini, Laura, and Prato, Maurizio

Subjects
Materials science, Biocompatibility, Carbon nanotubes, chemistry.chemical_element, Nanotechnology, 02 engineering and technology, Carbon nanotube, 010402 general chemistry, Settore BIO/09 - Fisiologia, 01 natural sciences, Carbon nanotubes, functionalization, neuronal networks, law.invention, Neuronal signaling, law, General Materials Science, functionalization, neuronal networks, Cultured neuronal network, Substrate (chemistry), General Chemistry, 021001 nanoscience & nanotechnology, 0104 chemical sciences, chemistry, Nerve cells, Surface modification, 0210 nano-technology, Carbon
Abstract

Pristine (as prepared) carbon nanotube (CNT) based substrates have been widely used to grow and interface neurons in culture. Nerve cells normally differentiate on CNTs and the synaptic networks, newly formed at the interface with this material, usually show an improved robustness in signal transfer. However manipulation of pristine CNTs is often prevented by their low dispersibility and tendency to aggregate in most solvents. This issue can be at least partially solved by adding solubilizing groups to the surface of CNT, which also helps improving their biocompatibility. It becomes therefore of crucial importance to determine whether chemically manipulated CNTs may maintain their performance in improving nerve signaling. Here we study and compare the impact in vitro on neuronal signaling of two classes of chemically modified multiwalled CNTs in reference to pristine CNTs, which are known to be a substrate able to boost neuronal growth and communication. We found that the extent of functionalization and the nature of the functional groups on MWNT sidewalls affect the conductivity and the biological effects of the final derivatives. This information is important for the future design of biointegrated devices.

Published
2016

22. Graphene Oxide Nanosheets and Neural System: From Synaptic Modulation to Neuroinflammation

Author

Loredana Casalis, Maurizio Prato, Rossana Rauti, Laura Ballerini, Neus Lozano Valdes, Mattia Musto, Kostas Kostarelos, Clara Ballerini, Musto, Mattia, Rauti, Rossana, Lozano Valdes, Neu, Ballerini, Clara, Casalis, Loredana, Prato, Maurizio, Kostarelos, Kosta, and Ballerini, Laura

Subjects
Materials science, Graphene, Biophysics, Oxide, law.invention, Synaptic modulation, chemistry.chemical_compound, chemistry, law, Neural system, Neuroinflammation
Published
2018

23. Early signs of motoneuron vulnerability in a disease model system: Characterization of transverse slice cultures of spinal cord isolated from embryonic ALS mice

Author

F. Mazzarol, M. Zatta, Micaela Grandolfo, Laura Ballerini, Daniela Avossa, Avossa, D, Grandolfo, M, Mazzarol, F, Zatta, M, and Ballerini, Laura

Subjects
Pathology, medicine.medical_specialty, Neurotoxins, Central nervous system, Excitotoxicity, Mice, Transgenic, Nerve Tissue Proteins, Biology, medicine.disease_cause, Settore BIO/09 - Fisiologia, Choline O-Acetyltransferase, Mice, Myelin, Organ Culture Techniques, SOD1 mutant, Microscopy, Electron, Transmission, Excitatory Amino Acid Agonists, selective vulnerability, medicine, Animals, Humans, Genetic Predisposition to Disease, Receptors, AMPA, Motor Neurons, electron microscopy, Glial fibrillary acidic protein, General Neuroscience, Amyotrophic Lateral Sclerosis, Calcium-Binding Proteins, ALS, excitotoxicity, organotypic culture, Wild type, Drug Tolerance, Motor neuron, Spinal cord, Immunohistochemistry, Mitochondria, Disease Models, Animal, medicine.anatomical_structure, Spinal Cord, Nerve Degeneration, Synapses, biology.protein, Immunostaining
Abstract

Mutations in the SOD1 gene are associated with familial amyotrophic lateral sclerosis. The mechanisms by which these mutations lead to cell loss within the spinal cord ventral horns are unknown. In the present report we used the G93A transgenic mouse model of amyotrophic lateral sclerosis to develop and characterize an in vitro tool for the investigation of subtle alterations of spinal tissue prior to frank neuronal degeneration. To this aim, we developed organotypic slice cultures from wild type and G93A embryonic spinal cords. We combined immunocytochemistry and electron microscopy techniques to compare wild type and G93A spinal cord tissues after 14 days of growth under standard in vitro conditions. By SMI32 and choline acetyl transferase immunostaining, the distribution and morphology of motoneurons were compared in the two culture groups. Wild type and mutant cultures displayed no differences in the analyzed parameters as well as in the number of motoneurons. Similar results were observed when glial fibrillary acidic protein and myelin basic protein-positive cells were examined. Cell types within the G93A slice underwent maturation and slices could be maintained in culture for at least 3 weeks when prepared from embryos. Electron microscopy investigation confirmed the absence of early signs of mitochondria vacuolization or protein aggregate formation in G93A ventral horns. However, a significantly different ratio between inhibitory and excitatory synapses was present in G93A cultures, when compared with wild type ones, suggesting the expression of subtle synaptic dysfunction in G93A cultured tissue. When compared with controls, G93A motoneurons exhibited increased vulnerability to AMPA glutamate receptor-mediated excitotoxic stress prior to clear disease appearance. This in vitro disease model may thus represent a valuable tool to test early mechanisms contributing to motoneuron degeneration and potential therapeutic molecular interventions.

Published
2006

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