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Your search keyword '"Bartholomew J. Naughton"' showing total 2 results
Start Over Author "Bartholomew J. Naughton" Publisher elsevier bv
2 results on '"Bartholomew J. Naughton"'

1. Analysis of programmed death-ligand 1 (PD-L1) expression, transforming growth factor (TGF)-β gene expression signatures (GES) and tumor-infiltrating immune cells (IC) in hepatocellular carcinoma (HCC): Rationale for targeting PD-L1- and TGF-β

Author

Eveline Frick-Krieger, Isabelle Dussault, Yue Zhang, Luigi Terracciano, Bartholomew J. Naughton, Christian Ihling, and P.A. Rolfe

Subjects
Hematology, Biology, medicine.disease, Ligand (biochemistry), Immune system, Oncology, Hepatocellular carcinoma, PD-L1, Gene expression, Cancer research, medicine, biology.protein, Pd l1 expression, Programmed death, Transforming growth factor
Published
2017

2. Fluorescence-based evaluation of shRNA efficacy

Author

Dawn D. Han, Howard H. Gu, and Bartholomew J. Naughton

Subjects
Molecular Sequence Data, Biophysics, CHO Cells, Biology, Biochemistry, Fluorescence, Article, Small hairpin RNA, Cricetulus, Genes, Reporter, RNA interference, Cricetinae, Gene Knockdown Techniques, Animals, Gene silencing, RNA, Messenger, RNA, Small Interfering, Molecular Biology, Gene, Messenger RNA, Gene knockdown, Base Sequence, RNA, Cell Biology, Molecular biology, Cell biology, Evaluation Studies as Topic
Abstract

RNA interference is a cellular mechanism regulating levels of mRNAs. It has been widely exploited to knock down specific protein targets. The selected interfering RNA sequence greatly influences its ability to knock down the target. Here we present a method for constructing multiple testing plasmids which express small hairpin RNAs (shRNA) targeting different regions of an mRNA. A simple fluorescence test in cultured cells allows convenient evaluation of mRNA knockdown by many different shRNAs on 96-well plates. We show that software predicted shRNAs have varying efficacies and only 2 of the 7 tested shRNAs significantly knocked down their targets.

Published
2011

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