1. Functional insights into the core-TFIIH from a comparative survey
- Author
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Odile Lecompte, Florence Bedez, Julie D. Thompson, Raymond Ripp, Benjamin Linard, Olivier Poch, Xavier Brochet, Dino Moras, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), and Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Transcription, Genetic ,Sequence analysis ,Biology ,Splicing ,[SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy ,Phylogenetic distribution ,CDK-activating kinase ,Evolution, Molecular ,03 medical and health sciences ,Transcription (biology) ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Genetics ,Animals ,Humans ,MESH: Animals ,Comparative genomic analysis ,MESH: Phylogeny ,MESH: Evolution, Molecular ,Phylogeny ,030304 developmental biology ,0303 health sciences ,MESH: Humans ,TFIIH ,MESH: Transcription Factor TFIIH ,MESH: Transcription, Genetic ,030302 biochemistry & molecular biology ,MESH: Multiprotein Complexes ,P34 ,MESH: Protein Subunits ,Cyclin-Dependent Kinases ,DNA-Binding Proteins ,Protein Subunits ,MESH: Cyclin-Dependent Kinases ,Multiprotein Complexes ,RNA splicing ,Proteome ,P34 2 ,Transcription factor II H ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,[INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM] ,Transcription ,Transcription Factor TFIIH ,Repair ,MESH: DNA-Binding Proteins - Abstract
International audience; TFIIH is a eukaryotic complex composed of two subcomplexes, the CAK (Cdk activating kinase) and the core-TFIIH. The core-TFIIH, composed of seven subunits (XPB, XPD, P62, P52, P44, P34, and P8), plays a crucial role in transcription and repair. Here, we performed an extended sequence analysis to establish the accurate phy-logenetic distribution of the core-TFIIH in 63 eukaryotic organisms. In spite of the high conservation of the seven subunits at the sequence and genomic levels, the non-enzymatic P8, P34, P52 and P62 are absent from one or a few unicellular species. To gain insight into their respective roles, we undertook a comparative genomic analysis of the whole proteome to identify the gene sets sharing similar presence/absence patterns. While little information was inferred for P8 and P62, our studies confirm the known role of P52 in repair and suggest for the first time the implication of the core TFIIH in mRNA splicing via P34.
- Published
- 2013
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