1. ROS and Oxidative Stress Are Elevated in Mitosis during Asynchronous Cell Cycle Progression and Are Exacerbated by Mitotic Arrest
- Author
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Brian A. Joughin, Jesse C. Patterson, Douglas A. Lauffenburger, Bert van de Kooij, Daniel Lim, Michael B. Yaffe, Massachusetts Institute of Technology. Department of Biology, Massachusetts Institute of Technology. Department of Biological Engineering, and Koch Institute for Integrative Cancer Research at MIT
- Subjects
chemistry.chemical_classification ,Reactive oxygen species ,Histology ,Cell Cycle ,Mitosis ,Cell Biology ,Cell cycle ,Mitotic arrest ,medicine.disease_cause ,Article ,8-Oxoguanine ,Pathology and Forensic Medicine ,Cell biology ,Oxidative Stress ,chemistry.chemical_compound ,chemistry ,Cancer cell ,medicine ,Humans ,Reactive Oxygen Species ,Oxidative stress ,Cysteine - Abstract
Although elevated levels of reactive oxygen species (ROS) have been observed in cancer cells and cancer cells aberrantly proliferate, it is not known whether the level of reactive oxygen species and the accumulation of oxidative damage to macromolecules vary across the cell cycle. Here, we measure the prevalence of reactive oxygen species and of biomolecule oxidation across the cell cycle in freely cycling cancer cells. We report that reactive oxygen species vary during the cell cycle and peak in mitosis, resulting in mitotic accumulation of oxidized protein cysteine residues. Prolonged mitotic arrest further increased the levels of ROS and the abundance of oxidatively damaged biomolecules, including cysteine-sulfenic-acid-containing proteins and 8-oxoguanine. These finding suggest that mitotic arrest agents may enhance the effects of ROS-dependent anticancer therapies. We studied connections between ROS and the cell cycle in unsynchronized cancer cells and using multiple independent assays found that ROS and oxidative damage to biomolecules are highest in mitosis and can be further enhanced by mitotic arrest., National Institutes of Health (U.S.) (Grant R01-GM104047), National Institutes of Health (U.S.) (Grant R01-ES015339), National Institutes of Health (U.S.) (Grant R35-ES028374), National Institutes of Health (U.S.) (Grant U54-CA112967), National Institutes of Health (U.S.) (Grant U54-CA217377), United States. Department of Defense. Peer-Reviewed Medical Research Program (Contract W81XWH-16-1-0464), Koch Institute Support Grant (P30-CA14051), Center for Environmental Health and Injury Control (U.S.) (Support Grant P30-ES002109)
- Published
- 2019