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The present study evaluates porcine pancreatic lipase (PPL) inhibitory potential of a phenolic fraction from the fermentation extract of endophytic fungus D. arengae. In absence of inhibitor (phenolic fraction), the initial K m and V max of PPL were found to be 3.83±0.94 µM and 11.54±0.54 µmole/min, respectively. While, in its presence the K m app and V max app was recorded as 7.48 µM and 10.6 µmole/min, respectively and were comparable to those obtained with standard, orlistat. Increase in the K m app and no change in the V max app in the presence of inhibitor (phenolic fraction) resulted in decrease in the specificity constant ( K cat / K m ). The inhibition constant ( K i ) for the phenol-rich fraction was recorded as 13.47±5.01 µg/ml, compared to 10.65±3.81 µM obtained for Orlistat. Thus, the phenol-rich fraction from endophytic D. arengae is able to inhibit PPL competitively as like Orlistat and holds promise as the potential drug candidate.

Present study evaluates antioxidative and erythrocyte membrane stabilizing potential of methanol extract of Carissa carandas leaves Cc(L)M and its purified fraction CM 1. Phytochemically Cc(L)M revealed presence of alkaloid, steroids, saponins and tanins. The results of antioxidant assays of Cc(L)M and CM 1 found to be dose dependent. However, in TLC bioautography, CM 1 showed yellow coloured scavenged band of DPPH reagent. The results revealed that CM 1 has significant (p≤0.05) free radical scavenging potential compared to Cc(L)M. The purified fraction CM 1 was found to be most potent with lowest EC 50 values for DPPH (546.4 µg/ml), OH - (498.5 µg/ml) radical scavenging and 57.48 µg/ml for reducing power assay. It also erythrocytes membrane stabilization effect with lowest EC 50 1.31 mg/ml as compared to Cc(L)M (2.56 mg/ml) and standard diclofenac sodium (1.00 mg/ml). The FT-IR and GC-HRMS analysis of CM 1 reveal the presence of steroid derivative 20-hydroxypregnan 18-oic acid.