1. Pathogenesis of Enterohemorrhagic Escherichia coli O157:H7 is mediated by the cytochrome P450 family in Caenorhabditis elegans animal model
- Author
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Hye Jin Choi, Bohyun Yun, Minho Song, Sangdon Ryu, Sangnam Oh, Mi Ri Park, Woong Ji Lee, Mi-Hwa Oh, Younghoon Kim, and Nam Su Oh
- Subjects
Transgene ,010401 analytical chemistry ,Virulence ,04 agricultural and veterinary sciences ,Biology ,medicine.disease_cause ,biology.organism_classification ,040401 food science ,01 natural sciences ,0104 chemical sciences ,law.invention ,Microbiology ,0404 agricultural biotechnology ,Immune system ,RNA interference ,law ,medicine ,Gene ,Escherichia coli ,Polymerase chain reaction ,Caenorhabditis elegans ,Food Science ,Biotechnology - Abstract
Foodborne pathogens, including enterohemorrhagic Escherichia coli (EHEC) O157:H7, may enter from the farm environment and foods via several different vectors and influence human health. Here, we employed Caenorhabditis elegans as a host model system and compared specific host responses during EHEC O157:H7 infection using whole-transcriptome analysis. To elucidate the immune pathways stimulated by EHEC O157:H7, we employed quantitative real-time polymerase chain reaction (qRT-PCR), transgenic worms, and RNAi. Whole-transcriptome analysis revealed that genes encoding the cytochrome P450 (CYP450) family were induced more than 10-fold during EHEC O157:H7 infection in C. elegans host models. Importantly, C. elegans mutants lacking CYP450 genes were highly susceptible to EHEC O157:H7 infection compared with wild-type N2 worms. Consistent with susceptibility tests, qRT-PCR results indicated that CYP450 loss-of-function mutations significantly affected the transcriptional induction of antimicrobial peptide genes, such as clec-60. Together, our results provide critical insights into host strategies for avoiding EHEC O157:H7 pathogenesis in the gastrointestinal tract via the cytochrome P450 family and highlights potential molecular targets for preventing the virulence of EHEC O157:H7 in foods.
- Published
- 2019