1. Silencing of CDC42 inhibits neuroblastoma cell proliferation and transformation
- Author
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Brian T. Craig, Jingbo Qiao, Sora Lee, Dai H. Chung, and Carmelle V. Romain
- Subjects
Cancer Research ,Programmed cell death ,CDC42 ,Biology ,Article ,Malignant transformation ,Proto-Oncogene Proteins c-myc ,Neuroblastoma ,Cell Line, Tumor ,medicine ,Humans ,Gene silencing ,cdc42 GTP-Binding Protein ,Cell Proliferation ,Oncogene Proteins ,N-Myc Proto-Oncogene Protein ,Cell growth ,Nuclear Proteins ,medicine.disease ,Cell biology ,Cell Transformation, Neoplastic ,Oncology ,Cell culture ,Cancer research ,Undifferentiated Neuroblastoma - Abstract
Cell division cycle 42 (CDC42), a small GTPase of the Rho-subfamily, regulates diverse cellular functions including proliferation, cytoskeletal rearrangement and even promotes malignant transformation. Here, we found that increased expression of CDC42 correlated with undifferentiated neuroblastoma as compared to a more benign phenotype. CDC42 inhibition decreased cell growth and soft agar colony formation, and increased cell death in BE(2)-C and BE(2)-M17 cell lines, but not in SK-N-AS. In addition, silencing of CDC42 decreased expression of N-myc in BE(2)-C and BE(2)-M17 cells. Our findings suggest that CDC42 may play a role in the regulation of aggressive neuroblastoma behavior.
- Published
- 2014
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