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RAPTOR, Resonance ionization spectroscopy And Purification Traps for Optimized spectRoscopy, is a new collinear resonance ionization spectroscopy device constructed at the Ion Guide Isotope Separator On-Line (IGISOL) facility at the University of Jyv\"askyl\"a, Finland. By operating at beam energies of under 10 keV, the footprint of the experiment is reduced compared to more traditional collinear laser spectroscopy beamlines. In addition, RAPTOR is coupled to the JYFLTRAP Penning trap mass spectrometer, opening a window to laser-assisted nuclear-state selective purification, serving not only the mass measurement program, but also supporting post-trap decay spectroscopy experiments. Finally, the low-energy ion beams used for RAPTOR will enable high-precision laser-radiofrequency double-resonance experiments, resulting in spectroscopy with linewidths below 1 MHz. In this contribution, the technical layout of RAPTOR and a selection of ion-beam optical simulations for the device are presented, along with a discussion of the current status of the commissioning experiments., Comment: EMIS 2022 conference, 4 figures

Exploiting gene delivery from the surfaces of bio-functionalized implants is a unique strategy to facilitate tissue regeneration and integration. However, it has been challenging, due to the difficulty for incorporation and delivey of sufficient payloads of plasmid DNA (pDNA) to cells from the implant surface allowing efficient gene expression. Herein, we describe a novel and simple coating approach using nanostructured silicate substituted calcium phosphate pDNA complexes termed as NanoSiCaPs, that preferentially adsorb onto titanium (Ti) substrates coated with poly (diallyldimethylammonium chloride) (PDADMAC). The Ti-polyelectrolyte NanoSiCaPs assemblies aptly called PNA, deliver pDNA to MC3T3-E1 cells (pre-osteoblast cell line), induce protein production, without eliciting any cytotoxicity, while simultaneously encouraging cell attachment on the substrate.

Mutations in glycosyltransferases, such as protein O-mannose N-acetylglucosaminyltransferase 1 (POMGnT1), causes disruptions of basement membranes (BMs) that results in neuronal ectopias and muscular dystrophy. While the mutations diminish dystroglycan-mediated cell-ECM interactions, the cause and mechanism of BM disruptions remain unclear. In this study, we established an in vitro model to measure BM assembly on the surface of neural stem cells. Compared to control cells, the rate of BM assembly on POMGnT1 knockout neural stem cells was significantly reduced. Further, immunofluorescence staining and quantitative proteomic analysis of the inner limiting membrane (ILM), a BM of the retina, revealed that laminin-111 and nidogen-1 were reduced in POMGnT1 knockout mice. Finally, atomic force microscopy showed that the ILM from POMGnT1 knockout mice was thinner with an altered surface topography. The results combined demonstrate that reduced levels of key BM components cause physical changes that weaken the BM in POMGnT1 knockout mice. These changes are caused by a reduced rate of BM assembly during the developmental expansion of the neural tissue.

Basement membranes (BMs) are physiologically insoluble extracellular matrix sheets present in all multicellular organisms. They play an important role in providing mechanical strength to tissues and regulating cell behavior. Proteomic analysis of BM proteins is challenged by their high molecular weights and extensive post-translational modifications. Here, we describe the direct analysis of an in vivo BM system using a mass spectrometry (MS) based proteomics approach. Retinal BMs were isolated from embryonic chick eyes. The BM macromolecules were deglycosylated and separated by low percentage gradient SDS PAGE, in-gel digested and analyzed by LC-MS/MS. This identified over 27 extracellular matrix proteins in the retinal BM. A semi-quantitative measure of protein abundance distinguished, nidogens-1 and -2, laminin subunits α1, α5, β2, and γ1, agrin, collagen XVIII, perlecan, FRAS1 and FREM2 as the most abundant BM protein components. Laminin subunits α3, β1, γ2, γ3 and collagen IV subunits α5 and α6 were minor constituents. To examine binding interactions that contribute to the stability of the retinal BM, we applied the LC-MS/MS based approach to detect potential BM complexes from the vitreous. Affinity-captured nidogen- and heparin-binding proteins from the vitreous contained >10 and >200 proteins respectively. Comparison of these protein lists with the retinal BM proteome reveals that glycosaminoglycan and nidogen binding interactions play a central role in the internal structure and formation of the retinal BM. In addition, we studied the biomechanical qualities of the retinal BM before and after deglycosylation using atomic force microscopy. These results show that the glycosaminoglycan side chains of the proteoglycans play a dominant role in regulating the thickness and elasticity of the BMs by binding water to the extracellular matrix. To our knowledge, this is the first large-scale investigation of an in vivo BM system using MS-based proteomics.

Basement membranes (BMs) are considered to be uniform, approximately 100 nm-thin extracellular matrix sheets that serve as a substrate for epithelial cells, endothelial cells and myotubes. To find out whether BMs maintain their ultrastructure, protein composition and biophysical properties throughout life the natural aging history of the human inner limiting membranes (ILM) was investigated. The ILM is a BM at the vitreal surface of the retina that connects the retina with the vitreous. Transmission electron microscopy (TEM) showed that the ILM steadily increases in thickness from 70 nm at fetal stages to several microns at age 90. By the age of 20, the ILM loses its laminated structure to become an amorphous and very irregular extracellular matrix layer. Atomic force microscopy (AFM) showed that the native, hydrated ILMs are on average 4-fold thicker than the dehydrated ILMs as seen by TEM and that their thickness is prominently determined by its water-binding proteoglycans. The morphological changes are accompanied by age-related changes in the biochemical composition, whereby the relative concentrations of collagen IV and agrin increase, and the concentration of laminin decreases with age. Force-indentation measurements by AFM also showed that ILMs become increasingly stiffer with advancing age. The data suggest that BMs from other human tissues may undergo similar age-related changes.

The $N=1$, $D=10$ Supergravity--Super--Yang--Mills (SUGRA-SYM) theory is plagued by ABBJ gauge and Lorentz anomalies which are cancelled via the Green-Schwarz anomaly cancellation mechanism. Due to the fact that the ABBJ anomalies are not invariant under supersymmetry (SUSY) transformations one concludes that the theory is plagued also by a SUSY anomaly. For the gauge groups $SO(32)$ and $E_8\times E_8$ we compute this SUSY anomaly, by solving a coupled cohomology problem, and we show that it can be cancelled by subtracting from the action the known Green--Schwarz counterterm, the same which cancels also the ABBJ anomaly, the expected result. Finally, we argue that the corresponding mechanism does not apply in the dual SUGRA-SYM, related to the heterotic five-brane., DFPD/94/TH/01, 11 pages, one reference corrected

It is nearly twenty years that there exist computer programs to reduce products of Lie algebra irreps. This is a contribution in the field that uses a modern computer language (``C'') in a highly structured and object-oriented way. This gives the benefits of high portability, efficiency, and makes it easier to include the functions in user programs. Characteristic of this set of routines is the {\it all-dynamic} approach for the use of memory, so that the package only uses the memory resources as needed., Comment: 18 pages

A novel X-ray imaging technology (AlluraClarity, Philips Healthcare, Best, The Netherlands) enables patient radiation dose reduction by a combination of advanced image noise reduction algorithms with state-of-the-art hardware and an anatomy-specific optimized full acquisition chain. The main purpose